Restriction map for
pFN21AB7873
(Circular) (Six-base) MAPSORT of: pFN21AB7873.seq Check: 986 from: 1 to: 6415
pFN21AB7873 6415 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2089 2870 3050 3059 2089
Size: 781 180 9 5445
Fragments arranged by size:
5445 781 180 9
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 3736 3736
Size: 6415
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2377 2660 3300 4478 5862 6130 1632
Size: 745 283 640 1178 1384 268 1917
Fragments arranged by size:
1917 1384 1178 745 640 283 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 2827 5314 6291 1203
Size: 1624 2487 977 1327
Fragments arranged by size:
2487 1624 1327 977
AgeI A'CCGG_T
Cuts at: 5246 5246
Size: 6415
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 2664 2754 1166
Size: 1498 90 4827
Fragments arranged by size:
4827 1498 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6415
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2751 2783 2751
Size: 32 6383
AlwNI CAG_nnn'CTG
Cuts at: 3051 3364 4158 5730 3051
Size: 313 794 1572 3736
Fragments arranged by size:
3736 1572 794 313
ApaI G_GGCC'C
Cuts at: 3295 3295
Size: 6415
ApaLI G'TGCA_C
Cuts at: 3017 4472 5628 3017
Size: 1455 1156 3804
Fragments arranged by size:
3804 1455 1156
ApoI r'AATT_y
Cuts at: 1493 3724 3950 4102 6211 1493
Size: 2231 226 152 2109 1697
Fragments arranged by size:
2231 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4969 160
Size: 872 3937 1606
Fragments arranged by size:
3937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2992 3740 1870
Size: 73 168 881 748 4545
Fragments arranged by size:
4545 881 748 168 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2638 2671 2638
Size: 33 6382
BamHI G'GATC_C
Cuts at: 3745 4112 3745
Size: 367 6048
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 3323 3482 3736 5191
Size: 325 217 627 1172 364 159 254 1455
Cuts at: 5191 618
Size: 1842
Fragments arranged by size:
1842 1455 1172 627 364 325 254 217
159
BanII G_rGCy'C
Cuts at: 1391 2569 2874 3295 3734 1391
Size: 1178 305 421 439 4072
Fragments arranged by size:
4072 1178 439 421 305
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3357 928
Size: 971 1458 3986
Fragments arranged by size:
3986 1458 971
BbvCI CC'TCA_GC
Cuts at: 3531 3531
Size: 6415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4307 5517 4307
Size: 1210 5205
BclI T'GATC_A
Cuts at: 1548 3309 1548
Size: 1761 4654
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3266 3437 3659
Size: 107 122 71 631 605 1594 171 222
Cuts at: 3659 5026 136
Size: 1367 1525
Fragments arranged by size:
1594 1525 1367 631 605 222 171 122
107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 3832 3832
Size: 6415
Bme1580I G_kGCm'C
Cuts at: 1790 2687 2777 3021 3106 3295 3328 4476 5632
Size: 897 90 244 85 189 33 1148 1156
Cuts at: 5632 1790
Size: 2573
Fragments arranged by size:
2573 1156 1148 897 244 189 90 85
33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6415
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3414 4146 5104
Size: 416 53 83 399 24 1997 732 958
Cuts at: 5104 442
Size: 1753
Fragments arranged by size:
1997 1753 958 732 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6415
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2438 3137 3635 3707 5075 1239
Size: 1199 699 498 72 1368 2579
Fragments arranged by size:
2579 1368 1199 699 498 72
Bpu10I CC'TnA_GC
Cuts at: 3531 3531
Size: 6415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3185 3523 4540 5405 5703 3185
Size: 338 1017 865 298 3897
Fragments arranged by size:
3897 1017 865 338 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2718 5078 882
Size: 617 1219 2360 2219
Fragments arranged by size:
2360 2219 1219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2920 3013 4206 493
Size: 711 1716 93 1193 2702
Fragments arranged by size:
2702 1716 1193 711 93
BsaBI GATnn'nnATC
Cuts at: 6409 6409
Size: 6415
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2524 4222 4604 5239
Size: 53 83 186 936 991 1698 382 635
Cuts at: 5239 275
Size: 1451
Fragments arranged by size:
1698 1451 991 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4848 5246 5520 5667 6078 4848
Size: 398 274 147 411 5185
Fragments arranged by size:
5185 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6190
Fragments arranged by size:
6190 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 3143 3200 3650 1186
Size: 1519 438 57 450 3951
Fragments arranged by size:
3951 1519 450 438 57
BseYI C'CCAG_C
Cuts at: 2249 2603 3256 3327 3390 5618 6114 2249
Size: 354 653 71 63 2228 496 2550
Fragments arranged by size:
2550 2228 653 496 354 71 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2006 2210 2418 2719 3629 1163
Size: 573 270 204 208 301 910 3949
Fragments arranged by size:
3949 910 573 301 270 208 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 4626 5654 1540
Size: 275 186 1864 761 1028 2301
Fragments arranged by size:
2301 1864 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2330 2569 3021 4476 4561 5632 2330
Size: 239 452 1455 85 1071 3113
Fragments arranged by size:
3113 1455 1071 452 239 85
BsmI GAATG_Cn'
Cuts at: 2495 3926 4019 2495
Size: 1431 93 4891
Fragments arranged by size:
4891 1431 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2531 2531
Size: 6415
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2569 2687 2777 2874 3021 3106
Size: 399 540 239 118 90 97 147 85
Cuts at: 3106 3295 3328 3734 4476 4561 5632 1391
Size: 189 33 406 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 406 399 239 189
147 118 97 90 85 85 33
BspEI T'CCGG_A
Cuts at: 6078 6078
Size: 6415
BspHI T'CATG_A
Cuts at: 2443 4304 6034 2443
Size: 1861 1730 2824
Fragments arranged by size:
2824 1861 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2089 2736 2870 3050 3059 3322 3363 3770
Size: 1245 647 134 180 9 263 41 407
Cuts at: 3770 844
Size: 3489
Fragments arranged by size:
3489 1245 647 407 263 180 134 41
9
BsrBI CCG'CTC
Cuts at: 1394 1823 2540 2796 4302 1394
Size: 429 717 256 1506 3507
Fragments arranged by size:
3507 1506 717 429 256
BsrDI GCAATG_nn'
Cuts at: 65 1248 3600 4910 5084 65
Size: 1183 2352 1310 174 1396
Fragments arranged by size:
2352 1396 1310 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3066 5059 5246 1700
Size: 110 1256 1993 187 2869
Fragments arranged by size:
2869 1993 1256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 4475 5487 4475
Size: 1012 5403
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3051 794
Size: 2257 4158
BstBI TT'CG_AA
Cuts at: 5219 5219
Size: 6415
BstEII G'GTnAC_C
Cuts at: 1184 2029 2576 3385 1184
Size: 845 547 809 4214
Fragments arranged by size:
4214 845 809 547
BstXI CCAn_nnnn'nTGG
Cuts at: 2257 3382 3649 2257
Size: 1125 267 5023
Fragments arranged by size:
5023 1125 267
BstYI r'GATC_y
Cuts at: 1972 3745 4112 4497 4514 5966 6052 6064 6410
Size: 1773 367 385 17 1452 86 12 346
Cuts at: 6410 1972
Size: 1977
Fragments arranged by size:
1977 1773 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2719 2719
Size: 6415
BtgI C'CryG_G
Cuts at: 513 1065 2004 2884 3106 3123 3465 513
Size: 552 939 880 222 17 342 3463
Fragments arranged by size:
3463 939 880 552 342 222 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3284 487
Size: 683 56 2058 3618
Fragments arranged by size:
3618 2058 683 56
BtsI GCAGTG_nn'
Cuts at: 2286 2412 3025 3106 3612 3654 3931 4725 4745
Size: 126 613 81 506 42 277 794 20
Cuts at: 4745 2286
Size: 3956
Fragments arranged by size:
3956 794 613 506 277 126 81 42
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6415
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2500 2535 529
Size: 35 1936 35 4409
Fragments arranged by size:
4409 1936 35 35
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DraIII CAC_nnn'GTG
Cuts at: 2029 2143 2308 3013 3016 2029
Size: 114 165 705 3 5428
Fragments arranged by size:
5428 705 165 114 3
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3697 5422 809
Size: 507 2381 1725 1802
Fragments arranged by size:
2381 1802 1725 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2337 2791 2881
Size: 54 1425 50 275 18 507 454 90
Cuts at: 2881 3084 3438 3579 3651 3660 3862 4751 6380
Size: 203 354 141 72 9 202 889 1629
Cuts at: 6380 8
Size: 43
Fragments arranged by size:
1629 1425 889 507 454 354 275 203
202 141 90 72 54 50 43 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2636 3234 3558 4345 1353
Size: 1283 598 324 787 3423
Fragments arranged by size:
3423 1283 787 598 324
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3183 5002 5376 5522 387
Size: 1064 138 1594 1819 374 146 1280
Fragments arranged by size:
1819 1594 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2567 2567
Size: 6415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2377 2438 2660 3137 3300 3635 3707
Size: 393 745 61 222 477 163 335 72
Cuts at: 3707 4478 5075 5862 6130 1239
Size: 771 597 787 268 1524
Fragments arranged by size:
1524 787 771 745 597 477 393 335
268 222 163 72 61
EcoNI CCTnn'n_nnAGG
Cuts at: 3227 3227
Size: 6415
EcoO109I rG'GnC_Cy
Cuts at: 2365 2853 3142 3291 3292 3361 2365
Size: 488 289 149 1 69 5419
Fragments arranged by size:
5419 488 289 149 69 1
EcoRI G'AATT_C
Cuts at: 3724 4102 3724
Size: 378 6037
FspI TGC'GCA
Cuts at: 2834 2924 4921 2834
Size: 90 1997 4328
Fragments arranged by size:
4328 1997 90
FspAI rTGC'GCAy
Cuts at: 2834 2924 2834
Size: 90 6325
HaeII r_GCGC'y
Cuts at: 1678 2901 3477 5562 1678
Size: 1223 576 2085 2531
Fragments arranged by size:
2531 2085 1223 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3237 3269 5056 5088 5130
Size: 32 135 32 1665 32 1787 32 42
Cuts at: 5130 5162 1373
Size: 32 2626
Fragments arranged by size:
2626 1787 1665 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3759 4005 669
Size: 988 281 1821 246 3079
Fragments arranged by size:
3079 1821 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2084 748
Size: 1336 5079
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KpnI G_GTAC'C
Cuts at: 3740 3740
Size: 6415
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2293 2464 3681 5529 5713 1305
Size: 988 171 1217 1848 184 2007
Fragments arranged by size:
2007 1848 1217 988 184 171
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3086 3440 3581 3653 10
Size: 54 1425 343 1254 354 141 72 2772
Fragments arranged by size:
2772 1425 1254 354 343 141 72 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 2590 2664 2680 2754 3380 3605
Size: 648 1408 16 74 16 74 626 225
Cuts at: 3605 3647 4373 4732 4891 518
Size: 42 726 359 159 2042
Fragments arranged by size:
2042 1408 726 648 626 359 225 159
74 74 42 16 16
MspA1I CmG'CkG
Cuts at: 1606 2194 2502 2607 2886 3613 3831 4508 5656
Size: 588 308 105 279 727 218 677 1148
Cuts at: 5656 5901 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 727 677 588 308 279 245
218 105
NcoI C'CATG_G
Cuts at: 513 1065 2004 3106 3465 513
Size: 552 939 1102 359 3463
Fragments arranged by size:
3463 1102 939 552 359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6415
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 2741 2831 3773 5318 1132
Size: 97 1512 90 942 1545 2229
Fragments arranged by size:
2229 1545 1512 942 97 90
PasI CC'CwG_GG
Cuts at: 1348 3364 1348
Size: 2016 4399
PciI A'CATG_T
Cuts at: 2827 5314 2827
Size: 2487 3928
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2308 1108
Size: 1200 5215
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PmlI CAC'GTG
Cuts at: 2920 3013 2920
Size: 93 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 5997 6029 4484
Size: 32 1481 32 4870
Fragments arranged by size:
4870 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2365
Size: 6415
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 3985 3985
Size: 6415
PspOMI G'GGCC_C
Cuts at: 3291 3291
Size: 6415
PspXI vC'TCGA_Gb
Cuts at: 2111 2992 2111
Size: 881 5534
PstI C_TGCA'G
Cuts at: 830 2733 3047 3181 3360 3767 830
Size: 1903 314 134 179 407 3478
Fragments arranged by size:
3478 1903 407 314 179 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
PvuII CAG'CTG
Cuts at: 2194 2607 3613 2194
Size: 413 1006 4996
Fragments arranged by size:
4996 1006 413
SacI G_AGCT'C
Cuts at: 2569 2569
Size: 6415
SacII CC_GC'GG
Cuts at: 2887 2887
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SapI GCTCTTCn'nnn_
Cuts at: 2636 2636
Size: 6415
SbfI CC_TGCA'GG
Cuts at: 3360 3767 3360
Size: 407 6008
ScaI AGT'ACT
Cuts at: 2990 4663 6375 2990
Size: 1673 1712 3030
Fragments arranged by size:
3030 1712 1673
SexAI A'CCwGG_T
Cuts at: 2139 2139
Size: 6415
SfcI C'TryA_G
Cuts at: 826 1045 2729 3043 3177 3356 3763 4898 5579
Size: 219 1684 314 134 179 407 1135 681
Cuts at: 5579 5770 826
Size: 191 1471
Fragments arranged by size:
1684 1471 1135 681 407 314 219 191
179 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 3659 3659
Size: 6415
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 2992 3164 3538 4519 5420
Size: 197 926 168 881 172 374 981 901
Cuts at: 5420 5682 820
Size: 262 1553
Fragments arranged by size:
1553 981 926 901 881 374 262 197
172 168
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 3773 1132
Size: 2641 3774
SspI AAT'ATT
Cuts at: 5 52 4339 5
Size: 47 4287 2081
Fragments arranged by size:
4287 2081 47
StuI AGG'CCT
Cuts at: 1517 2618 2708 1517
Size: 1101 90 5224
Fragments arranged by size:
5224 1101 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 3081 3106 3465 3854 513
Size: 552 939 1077 25 359 389 3074
Fragments arranged by size:
3074 1077 939 552 389 359 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 2852 3214 3404 3500 4442 4459
Size: 1255 665 607 362 190 96 942 17
Cuts at: 4459 4612 4797 5242 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 942 665 607 445 362 190
185 153 96 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2988 4661 6373
Size: 274 80 33 51 1443 1011 1673 1712
Cuts at: 6373 96
Size: 138
Fragments arranged by size:
1712 1673 1443 1011 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6375 6407 6375
Size: 32 6383
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6415
XbaI T'CTAG_A
Cuts at: 3751 3751
Size: 6415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2114 2276 2011
Size: 103 162 6150
Fragments arranged by size:
6150 162 103
XhoI C'TCGA_G
Cuts at: 1943 2111 2992 1943
Size: 168 881 5366
Fragments arranged by size:
5366 881 168
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 2978 4544 2978
Size: 1566 4849
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PsrI RsrII SanDI SfoI SrfI SwaI