Restriction map for
pFN21AB7872
(Circular) (Six-base) MAPSORT of: pFN21AB7872.seq Check: 7600 from: 1 to: 6613
pFN21AB7872 6613 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2842 3570 2842
Size: 728 5885
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2157 2574 4423 278
Size: 53 83 186 936 621 417 1849 2468
Fragments arranged by size:
2468 1849 936 621 417 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3956 1937
Size: 2019 4594
Acc65I G'GTAC_C
Cuts at: 3934 3934
Size: 6613
AclI AA'CG_TT
Cuts at: 1563 4740 5113 1563
Size: 3177 373 3063
Fragments arranged by size:
3177 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3059 3617 3668 4676 6060 6328 1632
Size: 1427 558 51 1008 1384 268 1917
Fragments arranged by size:
1917 1427 1384 1008 558 268 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6416
AflIII A'CryG_T
Cuts at: 1203 3063 5512 6489 1203
Size: 1860 2449 977 1327
Fragments arranged by size:
2449 1860 1327 977
AgeI A'CCGG_T
Cuts at: 5444 5444
Size: 6613
AhdI GACnn_n'nnGTC
Cuts at: 2329 5342 2329
Size: 3013 3600
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6613
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 6581
AlwNI CAG_nnn'CTG
Cuts at: 2522 2787 2857 2947 3157 3571 3646 3682 4356
Size: 265 70 90 210 414 75 36 674
Cuts at: 4356 5928 2522
Size: 1572 3207
Fragments arranged by size:
3207 1572 674 414 265 210 90 75
70 36
ApaI G_GGCC'C
Cuts at: 3345 3353 3595 3345
Size: 8 242 6363
Fragments arranged by size:
6363 242 8
ApaLI G'TGCA_C
Cuts at: 3254 3761 4670 5826 3254
Size: 507 909 1156 4041
Fragments arranged by size:
4041 1156 909 507
ApoI r'AATT_y
Cuts at: 1493 2747 3922 4148 4300 6409 1493
Size: 1254 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1254 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5167 160
Size: 872 4135 1606
Fragments arranged by size:
4135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 3353 3433 3605 3938 1870
Size: 73 657 753 80 172 333 4545
Fragments arranged by size:
4545 753 657 333 172 80 73
BamHI G'GATC_C
Cuts at: 3943 4310 3943
Size: 367 6246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2338 2716 3026 3145
Size: 325 217 627 252 299 378 310 119
Cuts at: 3145 3934 5389 618
Size: 789 1455 1842
Fragments arranged by size:
1842 1455 789 627 378 325 310 299
252 217 119
BanII G_rGCy'C
Cuts at: 1391 2066 2601 2960 3005 3345 3353 3595 3932
Size: 675 535 359 45 340 8 242 337
Cuts at: 3932 1391
Size: 4072
Fragments arranged by size:
4072 675 535 359 340 337 242 45
8
BbeI G_GCGC'C
Cuts at: 2342 2342
Size: 6613
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2149 928
Size: 971 250 5392
Fragments arranged by size:
5392 971 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2071 2105 4804 4838 1903
Size: 34 134 34 2699 34 3678
Fragments arranged by size:
3678 2699 134 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4505 5715 4505
Size: 1210 5403
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6613
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2531 3813 5224
Size: 107 122 71 631 605 859 1282 1411
Cuts at: 5224 136
Size: 1525
Fragments arranged by size:
1525 1411 1282 859 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6608 6608
Size: 6613
BlpI GC'TnA_GC
Cuts at: 2207 4030 2207
Size: 1823 4790
Bme1580I G_kGCm'C
Cuts at: 1790 2042 2286 2721 3258 3345 3353 3595 3765
Size: 252 244 435 537 87 8 242 170
Cuts at: 3765 4674 5830 1790
Size: 909 1156 2573
Fragments arranged by size:
2573 1156 909 537 435 252 244 242
170 87 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6613
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2005 2095 2188
Size: 416 53 83 399 24 588 90 93
Cuts at: 2188 3415 4344 5302 442
Size: 1227 929 958 1753
Fragments arranged by size:
1753 1227 958 929 588 416 399 93
90 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2186 2616 2855 3149 5273 1239
Size: 841 106 430 239 294 2124 2579
Fragments arranged by size:
2579 2124 841 430 294 239 106
Bpu10I CC'TnA_GC
Cuts at: 3724 3724
Size: 6613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4738 5603 5901 4738
Size: 865 298 5450
Fragments arranged by size:
5450 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3436 5276 882
Size: 617 1937 1840 2219
Fragments arranged by size:
2219 1937 1840 617
BsaAI yAC'GTr
Cuts at: 493 1204 2563 3066 4404 493
Size: 711 1359 503 1338 2702
Fragments arranged by size:
2702 1359 1338 711 503
BsaBI GATnn'nnATC
Cuts at: 6607 6607
Size: 6613
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 2339 2571 4420
Size: 53 83 186 936 621 185 232 1849
Cuts at: 4420 4802 5437 275
Size: 382 635 1451
Fragments arranged by size:
1849 1451 936 635 621 382 232 186
185 83 53
BsaWI w'CCGG_w
Cuts at: 5046 5444 5718 5865 6276 5046
Size: 398 274 147 411 5383
Fragments arranged by size:
5383 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2942 2972 1178
Size: 30 165 30 1539 30 4819
Fragments arranged by size:
4819 1539 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3902 1186
Size: 2716 3897
BseYI C'CCAG_C
Cuts at: 2309 2857 3476 5816 6312 2309
Size: 548 619 2340 496 2610
Fragments arranged by size:
2610 2340 619 548 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3430 1163
Size: 573 1694 4346
Fragments arranged by size:
4346 1694 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 4063 4824 5852 1540
Size: 275 186 907 1155 761 1028 2301
Fragments arranged by size:
2301 1155 1028 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2055 2066 2271 2562 2601 2960 3258 3765 4674
Size: 11 205 291 39 359 298 507 909
Cuts at: 4674 4759 5830 2055
Size: 85 1071 2838
Fragments arranged by size:
2838 1071 909 507 359 298 291 205
85 39 11
BsmI GAATG_Cn'
Cuts at: 4124 4217 4124
Size: 93 6520
BsmBI CGTCTCn'nnnn_
Cuts at: 3048 3048
Size: 6613
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2055 2066 2271 2286 2562 2601
Size: 399 252 13 11 205 15 276 39
Cuts at: 2601 2721 2960 3005 3258 3345 3353 3595 3765
Size: 120 239 45 253 87 8 242 170
Cuts at: 3765 3932 4674 4759 5830 1391
Size: 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 253 252 242
239 205 170 167 120 87 85 45
39 15 13 11 8
BspEI T'CCGG_A
Cuts at: 6276 6276
Size: 6613
BspHI T'CATG_A
Cuts at: 4502 6232 4502
Size: 1730 4883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2842 3570 3858 3968 844
Size: 1998 728 288 110 3489
Fragments arranged by size:
3489 1998 728 288 110
BsrBI CCG'CTC
Cuts at: 1394 1823 2443 3690 4500 1394
Size: 429 620 1247 810 3507
Fragments arranged by size:
3507 1247 810 620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5108 5282 65
Size: 1183 3860 174 1396
Fragments arranged by size:
3860 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2335 2696 2805 2865 3029 3229 5257
Size: 110 525 361 109 60 164 200 2028
Cuts at: 5257 5444 1700
Size: 187 2869
Fragments arranged by size:
2869 2028 525 361 200 187 164 110
109 60
BsrGI T'GTAC_A
Cuts at: 96 2763 96
Size: 2667 3946
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6613
BssSI C'ACGA_G
Cuts at: 2594 4673 5685 2594
Size: 2079 1012 3522
Fragments arranged by size:
3522 2079 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3682 794
Size: 2888 3725
BstBI TT'CG_AA
Cuts at: 5417 5417
Size: 6613
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6613
BstYI r'GATC_y
Cuts at: 1972 3102 3600 3906 3943 4310 4695 4712 6164
Size: 1130 498 306 37 367 385 17 1452
Cuts at: 6164 6250 6262 6608 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1130 498 385 367 346 306
86 37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2217 3345 3370 3426 3799 513
Size: 552 939 213 1128 25 56 373 3327
Fragments arranged by size:
3327 1128 939 552 373 213 56 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5874
Fragments arranged by size:
5874 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 3387 3442 4129 4923 4943 2520
Size: 867 55 687 794 20 4190
Fragments arranged by size:
4190 867 794 687 55 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6578
DraI TTT'AAA
Cuts at: 3917 3917
Size: 6613
DraIII CAC_nnn'GTG
Cuts at: 3382 3382
Size: 6613
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5620 809
Size: 507 4304 1802
Fragments arranged by size:
4304 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2247 2412 2899
Size: 54 1425 50 275 18 417 165 487
Cuts at: 2899 3373 3702 4060 4949 6578 8
Size: 474 329 358 889 1629 43
Fragments arranged by size:
1629 1425 889 487 474 417 358 329
275 165 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4060 1537
Size: 275 2248 4090
Fragments arranged by size:
4090 2248 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4543 1353
Size: 3190 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2148 2950 3441 3848 5200 5574
Size: 1064 138 559 802 491 407 1352 374
Cuts at: 5574 5720 387
Size: 146 1280
Fragments arranged by size:
1352 1280 1064 802 559 491 407 374
146 138
EcoICRI GAG'CTC
Cuts at: 2064 2599 2958 2064
Size: 535 359 5719
Fragments arranged by size:
5719 535 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2186 2616 2855 3059 3149 3617
Size: 393 448 106 430 239 204 90 468
Cuts at: 3617 3668 4676 5273 6060 6328 1239
Size: 51 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 787 597 468 448 430 393
268 239 204 106 90 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3650 3650
Size: 6613
EcoO109I rG'GnC_Cy
Cuts at: 2944 3341 3350 3438 3583 3591 3592 3634 3643
Size: 397 9 88 145 8 1 42 9
Cuts at: 3643 2944
Size: 5914
Fragments arranged by size:
5914 397 145 88 42 9 9 8
1
EcoRI G'AATT_C
Cuts at: 2747 3922 4300 2747
Size: 1175 378 5060
Fragments arranged by size:
5060 1175 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 6613
FspI TGC'GCA
Cuts at: 5119 5119
Size: 6613
HaeII r_GCGC'y
Cuts at: 1678 2342 3420 3742 5760 1678
Size: 664 1078 322 2018 2531
Fragments arranged by size:
2531 2018 1078 664 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 3187 3219 5254
Size: 32 135 32 743 32 840 32 2035
Cuts at: 5254 5286 5328 5360 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2035 840 743 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3286 3957 4203 669
Size: 988 281 1348 671 246 3079
Fragments arranged by size:
3079 1348 988 671 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6613
HpaI GTT'AAC
Cuts at: 4203 4203
Size: 6613
KasI G'GCGC_C
Cuts at: 2338 2338
Size: 6613
KpnI G_GTAC'C
Cuts at: 3938 3938
Size: 6613
MfeI C'AATT_G
Cuts at: 4212 4212
Size: 6613
MluI A'CGCG_T
Cuts at: 6489 6489
Size: 6613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2379 5727 5911 1305
Size: 1074 3348 184 2007
Fragments arranged by size:
3348 2007 1074 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3375 10
Size: 54 1425 343 1543 3248
Fragments arranged by size:
3248 1543 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 4571 4930 5089 518
Size: 648 1485 1920 359 159 2042
Fragments arranged by size:
2042 1920 1485 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2787 3292 3682 3745 3801 4029 4706 5854
Size: 1181 505 390 63 56 228 677 1148
Cuts at: 5854 6099 1606
Size: 245 2120
Fragments arranged by size:
2120 1181 1148 677 505 390 245 228
63 56
NaeI GCC'GGC
Cuts at: 2337 2337
Size: 6613
NarI GG'CG_CC
Cuts at: 2339 2339
Size: 6613
NcoI C'CATG_G
Cuts at: 513 1065 2004 2217 3370 3426 513
Size: 552 939 213 1153 56 3700
Fragments arranged by size:
3700 1153 939 552 213 56
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6613
NgoMIV G'CCGG_C
Cuts at: 2335 2335
Size: 6613
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6613
NotI GC'GGCC_GC
Cuts at: 4060 4060
Size: 6613
NspI r_CATG'y
Cuts at: 1132 1229 2481 3971 5516 1132
Size: 97 1252 1490 1545 2229
Fragments arranged by size:
2229 1545 1490 1252 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6613
PciI A'CATG_T
Cuts at: 5512 5512
Size: 6613
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3295 3427 1108
Size: 2187 132 4294
Fragments arranged by size:
4294 2187 132
PmeI GTTT'AAAC
Cuts at: 3917 3917
Size: 6613
PmlI CAC'GTG
Cuts at: 2563 2563
Size: 6613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 4682 4714 6195 6227 2942
Size: 32 1708 32 1481 32 3328
Fragments arranged by size:
3328 1708 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3438 3583 3643 3438
Size: 145 60 6408
Fragments arranged by size:
6408 145 60
PshAI GACnn'nnGTC
Cuts at: 1613 2570 1613
Size: 957 5656
PsiI TTA'TAA
Cuts at: 4183 4183
Size: 6613
PspOMI G'GGCC_C
Cuts at: 3341 3349 3591 3341
Size: 8 242 6363
Fragments arranged by size:
6363 242 8
PstI C_TGCA'G
Cuts at: 830 3567 3642 3738 3965 830
Size: 2737 75 96 227 3478
Fragments arranged by size:
3478 2737 227 96 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6613
PvuII CAG'CTG
Cuts at: 2787 3292 3682 2787
Size: 505 390 5718
Fragments arranged by size:
5718 505 390
SacI G_AGCT'C
Cuts at: 2066 2601 2960 2066
Size: 535 359 5719
Fragments arranged by size:
5719 535 359
SacII CC_GC'GG
Cuts at: 3802 3802
Size: 6613
SalI G'TCGA_C
Cuts at: 1936 3955 1936
Size: 2019 4594
SanDI GG'GwC_CC
Cuts at: 3438 3438
Size: 6613
SbfI CC_TGCA'GG
Cuts at: 3642 3965 3642
Size: 323 6290
ScaI AGT'ACT
Cuts at: 4861 6573 4861
Size: 1712 4901
SfcI C'TryA_G
Cuts at: 826 1045 3191 3209 3563 3638 3664 3734 3961
Size: 219 2146 18 354 75 26 70 227
Cuts at: 3961 5096 5777 5968 826
Size: 1135 681 191 1471
Fragments arranged by size:
2146 1471 1135 681 354 227 219 191
75 70 26 18
SfoI GGC'GCC
Cuts at: 2340 2340
Size: 6613
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
SgrAI Cr'CCGG_yG
Cuts at: 1700 2335 1700
Size: 635 5978
SmaI CCC'GGG
Cuts at: 1872 3355 3940 1872
Size: 1483 585 4545
Fragments arranged by size:
4545 1483 585
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4717 5618 5880 820
Size: 197 926 2774 901 262 1553
Fragments arranged by size:
2774 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4404 493
Size: 3911 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6613
SphI G_CATG'C
Cuts at: 1132 3971 1132
Size: 2839 3774
SspI AAT'ATT
Cuts at: 5 52 4537 5
Size: 47 4485 2081
Fragments arranged by size:
4485 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6613
StyI C'CwwG_G
Cuts at: 513 1065 2004 2217 3022 3370 3426 3538 4052
Size: 552 939 213 805 348 56 112 514
Cuts at: 4052 513
Size: 3074
Fragments arranged by size:
3074 939 805 552 514 348 213 112
56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 2582 2922 3346 4640 4657 4810
Size: 1255 723 279 340 424 1294 17 153
Cuts at: 4810 4995 5440 325
Size: 185 445 1498
Fragments arranged by size:
1498 1294 1255 723 445 424 340 279
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2763 4859 6571
Size: 274 80 33 51 1443 786 2096 1712
Cuts at: 6571 96
Size: 138
Fragments arranged by size:
2096 1712 1443 786 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6573 6605 6573
Size: 32 6581
Tth111I GACn'n_nGTC
Cuts at: 1316 2690 1316
Size: 1374 5239
XbaI T'CTAG_A
Cuts at: 3949 3949
Size: 6613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3583 3583
Size: 6613
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6613
XmaI C'CCGG_G
Cuts at: 1870 3353 3938 1870
Size: 1483 585 4545
Fragments arranged by size:
4545 1483 585
XmnI GAAnn'nnTTC
Cuts at: 2140 4742 2140
Size: 2602 4011
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2155 2572 4421 276
Size: 53 83 186 936 621 417 1849 2468
Fragments arranged by size:
2468 1849 936 621 417 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI
PacI PfoI PspXI PsrI RsrII SapI SexAI SfiI
SrfI SwaI