(Circular) (Six-base) MAPSORT of: pFN21AB7863.seq Check: 5230 from: 1 to: 6367 pFN21AB7863 6367 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4177 278 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2643 3710 1937 Size: 706 1067 4594 Fragments arranged by size: 4594 1067 706 Acc65I G'GTAC_C Cuts at: 3688 3688 Size: 6367 AclI AA'CG_TT Cuts at: 1563 4494 4867 1563 Size: 2931 373 3063 Fragments arranged by size: 3063 2931 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2351 2452 2605 2705 3270 3442 4430 5814 Size: 719 101 153 100 565 172 988 1384 Cuts at: 5814 6082 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 988 719 565 268 172 153 101 100 AflII C'TTAA_G Cuts at: 820 1017 2486 820 Size: 197 1469 4701 Fragments arranged by size: 4701 1469 197 AflIII A'CryG_T Cuts at: 1203 3545 5266 6243 1203 Size: 2342 1721 977 1327 Fragments arranged by size: 2342 1721 1327 977 AgeI A'CCGG_T Cuts at: 5198 5198 Size: 6367 AhdI GACnn_n'nnGTC Cuts at: 5096 5096 Size: 6367 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6367 AlwNI CAG_nnn'CTG Cuts at: 2015 4110 5682 2015 Size: 2095 1572 2700 Fragments arranged by size: 2700 2095 1572 ApaLI G'TGCA_C Cuts at: 4424 5580 4424 Size: 1156 5211 ApoI r'AATT_y Cuts at: 1493 3396 3564 3660 3676 3902 4054 6163 1493 Size: 1903 168 96 16 226 152 2109 1697 Fragments arranged by size: 2109 1903 1697 226 168 152 96 16 AseI AT'TA_AT Cuts at: 160 1032 2706 4921 160 Size: 872 1674 2215 1606 Fragments arranged by size: 2215 1674 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 AvaI C'yCGr_G Cuts at: 1870 1943 3049 3692 1870 Size: 73 1106 643 4545 Fragments arranged by size: 4545 1106 643 73 BamHI G'GATC_C Cuts at: 3697 4064 3697 Size: 367 6000 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3688 5143 618 Size: 325 217 627 1901 1455 1842 Fragments arranged by size: 1901 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3686 1391 Size: 2295 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2458 3655 928 Size: 971 559 1197 3640 Fragments arranged by size: 3640 1197 971 559 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4558 4592 1903 Size: 34 2621 34 3678 Fragments arranged by size: 3678 2621 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2397 3306 4259 5469 2397 Size: 909 953 1210 3295 Fragments arranged by size: 3295 1210 953 909 BclI T'GATC_A Cuts at: 1548 1548 Size: 6367 BfrBI ATG'CAT Cuts at: 3064 3064 Size: 6367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4978 136 Size: 107 122 71 631 605 3306 1525 Fragments arranged by size: 3306 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6362 6362 Size: 6367 BlpI GC'TnA_GC Cuts at: 3784 3784 Size: 6367 Bme1580I G_kGCm'C Cuts at: 1790 3337 4428 5584 1790 Size: 1547 1091 1156 2573 Fragments arranged by size: 2573 1547 1156 1091 BmgBI CAC'GTC Cuts at: 1318 2212 1318 Size: 894 5473 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2045 2262 2347 Size: 416 53 83 399 24 628 217 85 Cuts at: 2347 2922 4098 5056 442 Size: 575 1176 958 1753 Fragments arranged by size: 1753 1176 958 628 575 416 399 217 85 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6367 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2339 2590 2771 2339 Size: 251 181 5935 Fragments arranged by size: 5935 251 181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 3018 3186 3438 3522 5027 1239 Size: 1001 778 168 252 84 1505 2579 Fragments arranged by size: 2579 1505 1001 778 252 168 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4492 5357 5655 4492 Size: 865 298 5204 Fragments arranged by size: 5204 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5030 882 Size: 617 3531 2219 Fragments arranged by size: 3531 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2497 3546 4158 493 Size: 711 1293 1049 612 2702 Fragments arranged by size: 2702 1293 1049 711 612 BsaBI GATnn'nnATC Cuts at: 2115 6361 2115 Size: 4246 2121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4174 4556 5191 275 Size: 53 83 186 936 2641 382 635 1451 Fragments arranged by size: 2641 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4800 5198 5472 5619 6030 4800 Size: 398 274 147 411 5137 Fragments arranged by size: 5137 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6142 Fragments arranged by size: 6142 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2331 1186 Size: 994 151 5222 Fragments arranged by size: 5222 994 151 BseYI C'CCAG_C Cuts at: 2878 5570 6066 2878 Size: 2692 496 3179 Fragments arranged by size: 3179 2692 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5794 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3817 4578 5606 1540 Size: 275 186 1816 761 1028 2301 Fragments arranged by size: 2301 1816 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4428 4513 5584 4428 Size: 85 1071 5211 Fragments arranged by size: 5211 1071 85 BsmI GAATG_Cn' Cuts at: 3524 3878 3971 3524 Size: 354 93 5920 Fragments arranged by size: 5920 354 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3337 3686 4428 4513 5584 1391 Size: 399 1547 349 742 85 1071 2174 Fragments arranged by size: 2174 1547 1071 742 399 349 85 BspEI T'CCGG_A Cuts at: 6030 6030 Size: 6367 BspHI T'CATG_A Cuts at: 2062 2524 3568 4256 5986 2062 Size: 462 1044 688 1730 2443 Fragments arranged by size: 2443 1730 1044 688 462 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3722 844 Size: 2878 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4254 1394 Size: 429 2431 3507 Fragments arranged by size: 3507 2431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2459 3617 4862 5036 65 Size: 1183 1211 1158 1245 174 1396 Fragments arranged by size: 1396 1245 1211 1183 1158 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5011 5198 1700 Size: 110 3201 187 2869 Fragments arranged by size: 3201 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6367 BssSI C'ACGA_G Cuts at: 4427 5439 4427 Size: 1012 5355 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6367 BstBI TT'CG_AA Cuts at: 3646 5171 3646 Size: 1525 4842 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6367 BstYI r'GATC_y Cuts at: 1972 3697 4064 4449 4466 5918 6004 6016 6362 Size: 1725 367 385 17 1452 86 12 346 Cuts at: 6362 1972 Size: 1977 Fragments arranged by size: 1977 1725 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2644 2644 Size: 6367 BtgI C'CryG_G Cuts at: 513 1065 2004 2026 513 Size: 552 939 22 4854 Fragments arranged by size: 4854 939 552 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5628 Fragments arranged by size: 5628 683 56 BtsI GCAGTG_nn' Cuts at: 3883 4677 4697 3883 Size: 794 20 5553 Fragments arranged by size: 5553 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3511 3546 529 Size: 35 2947 35 3350 Fragments arranged by size: 3350 2947 35 35 DraI TTT'AAA Cuts at: 3671 3671 Size: 6367 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5374 809 Size: 507 4058 1802 Fragments arranged by size: 4058 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 3814 4703 Size: 54 1425 50 275 18 408 1576 889 Cuts at: 4703 6332 8 Size: 1629 43 Fragments arranged by size: 1629 1576 1425 889 408 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3814 1537 Size: 275 2002 4090 Fragments arranged by size: 4090 2002 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 3246 3582 4297 1353 Size: 936 957 336 715 3423 Fragments arranged by size: 3423 957 936 715 336 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4954 5328 5474 387 Size: 1064 138 3365 374 146 1280 Fragments arranged by size: 3365 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2351 2452 2605 2705 3018 3186 Size: 393 608 111 101 153 100 313 168 Cuts at: 3186 3270 3438 3442 3522 4430 5027 5814 6082 Size: 84 168 4 80 908 597 787 268 Cuts at: 6082 1239 Size: 1524 Fragments arranged by size: 1524 908 787 608 597 393 313 268 168 168 153 111 101 100 84 80 4 EcoO109I rG'GnC_Cy Cuts at: 2047 2047 Size: 6367 EcoRI G'AATT_C Cuts at: 3564 3676 4054 3564 Size: 112 378 5877 Fragments arranged by size: 5877 378 112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2570 2570 Size: 6367 FspI TGC'GCA Cuts at: 4873 4873 Size: 6367 HaeII r_GCGC'y Cuts at: 1678 5514 1678 Size: 3836 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 5008 5040 5082 Size: 32 135 32 669 32 2735 32 42 Cuts at: 5082 5114 1373 Size: 32 2626 Fragments arranged by size: 2735 2626 669 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3711 3957 669 Size: 988 281 1773 246 3079 Fragments arranged by size: 3079 1773 988 281 246 HindIII A'AGCT_T Cuts at: 748 3621 748 Size: 2873 3494 HpaI GTT'AAC Cuts at: 3957 3957 Size: 6367 KpnI G_GTAC'C Cuts at: 3692 3692 Size: 6367 MfeI C'AATT_G Cuts at: 2408 3966 2408 Size: 1558 4809 MluI A'CGCG_T Cuts at: 6243 6243 Size: 6367 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 2988 5481 5665 1305 Size: 960 723 2493 184 2007 Fragments arranged by size: 2493 2007 960 723 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2240 10 Size: 54 1425 343 408 4137 Fragments arranged by size: 4137 1425 408 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2677 2929 4325 4684 4843 518 Size: 648 1511 252 1396 359 159 2042 Fragments arranged by size: 2042 1511 1396 648 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2012 3783 4460 5608 5853 1606 Size: 406 1771 677 1148 245 2120 Fragments arranged by size: 2120 1771 1148 677 406 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2026 513 Size: 552 939 22 4854 Fragments arranged by size: 4854 939 552 22 NdeI CA'TA_TG Cuts at: 387 2730 2926 387 Size: 2343 196 3828 Fragments arranged by size: 3828 2343 196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6367 NotI GC'GGCC_GC Cuts at: 3814 3814 Size: 6367 NsiI A_TGCA'T Cuts at: 3066 3066 Size: 6367 NspI r_CATG'y Cuts at: 1132 1229 2902 3068 3322 3406 3725 5270 1132 Size: 97 1673 166 254 84 319 1545 2229 Fragments arranged by size: 2229 1673 1545 319 254 166 97 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6367 PciI A'CATG_T Cuts at: 5266 5266 Size: 6367 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6367 PmeI GTTT'AAAC Cuts at: 3671 3671 Size: 6367 PmlI CAC'GTG Cuts at: 2497 3546 2497 Size: 1049 5318 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4436 4468 5949 5981 4436 Size: 32 1481 32 4822 Fragments arranged by size: 4822 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2047 Size: 6367 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6367 PsiI TTA'TAA Cuts at: 3937 3937 Size: 6367 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2494 2526 2494 Size: 32 6335 PstI C_TGCA'G Cuts at: 830 3719 830 Size: 2889 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6367 PvuII CAG'CTG Cuts at: 2012 2012 Size: 6367 SalI G'TCGA_C Cuts at: 1936 3709 1936 Size: 1773 4594 SanDI GG'GwC_CC Cuts at: 2047 2047 Size: 6367 SbfI CC_TGCA'GG Cuts at: 3719 3719 Size: 6367 ScaI AGT'ACT Cuts at: 3047 4615 6327 3047 Size: 1568 1712 3087 Fragments arranged by size: 3087 1712 1568 SfcI C'TryA_G Cuts at: 826 1045 2203 2869 3715 4850 5531 5722 826 Size: 219 1158 666 846 1135 681 191 1471 Fragments arranged by size: 1471 1158 1135 846 681 666 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6367 SmaI CCC'GGG Cuts at: 1872 3694 1872 Size: 1822 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 4471 5372 5634 820 Size: 197 926 543 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 4158 493 Size: 3665 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6367 SphI G_CATG'C Cuts at: 1132 3725 1132 Size: 2593 3774 SspI AAT'ATT Cuts at: 5 52 2638 4291 5 Size: 47 2586 1653 2081 Fragments arranged by size: 2586 2081 1653 47 StuI AGG'CCT Cuts at: 1517 3260 3455 1517 Size: 1743 195 4429 Fragments arranged by size: 4429 1743 195 StyI C'CwwG_G Cuts at: 513 1065 2004 2026 2326 3806 513 Size: 552 939 22 300 1480 3074 Fragments arranged by size: 3074 1480 939 552 300 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 3401 4394 4411 4564 4749 5194 Size: 1255 594 1227 993 17 153 185 445 Cuts at: 5194 325 Size: 1498 Fragments arranged by size: 1498 1255 1227 993 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2510 3045 4613 Size: 274 80 33 51 1443 533 535 1568 Cuts at: 4613 6325 96 Size: 1712 138 Fragments arranged by size: 1712 1568 1443 535 533 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 6327 6359 2076 Size: 32 4219 32 2084 Fragments arranged by size: 4219 2084 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6367 XbaI T'CTAG_A Cuts at: 3703 3703 Size: 6367 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6367 XmaI C'CCGG_G Cuts at: 1870 3692 1870 Size: 1822 4545 XmnI GAAnn'nnTTC Cuts at: 2951 3035 3119 3539 3660 4496 2951 Size: 84 84 420 121 836 4822 Fragments arranged by size: 4822 836 420 121 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4175 276 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI