Restriction map for
pFN21AB7863
(Circular) (Six-base) MAPSORT of: pFN21AB7863.seq Check: 5230 from: 1 to: 6367
pFN21AB7863 6367 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4177 278
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2643 3710 1937
Size: 706 1067 4594
Fragments arranged by size:
4594 1067 706
Acc65I G'GTAC_C
Cuts at: 3688 3688
Size: 6367
AclI AA'CG_TT
Cuts at: 1563 4494 4867 1563
Size: 2931 373 3063
Fragments arranged by size:
3063 2931 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2351 2452 2605 2705 3270 3442 4430 5814
Size: 719 101 153 100 565 172 988 1384
Cuts at: 5814 6082 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 988 719 565 268 172 153
101 100
AflII C'TTAA_G
Cuts at: 820 1017 2486 820
Size: 197 1469 4701
Fragments arranged by size:
4701 1469 197
AflIII A'CryG_T
Cuts at: 1203 3545 5266 6243 1203
Size: 2342 1721 977 1327
Fragments arranged by size:
2342 1721 1327 977
AgeI A'CCGG_T
Cuts at: 5198 5198
Size: 6367
AhdI GACnn_n'nnGTC
Cuts at: 5096 5096
Size: 6367
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6367
AlwNI CAG_nnn'CTG
Cuts at: 2015 4110 5682 2015
Size: 2095 1572 2700
Fragments arranged by size:
2700 2095 1572
ApaLI G'TGCA_C
Cuts at: 4424 5580 4424
Size: 1156 5211
ApoI r'AATT_y
Cuts at: 1493 3396 3564 3660 3676 3902 4054 6163 1493
Size: 1903 168 96 16 226 152 2109 1697
Fragments arranged by size:
2109 1903 1697 226 168 152 96 16
AseI AT'TA_AT
Cuts at: 160 1032 2706 4921 160
Size: 872 1674 2215 1606
Fragments arranged by size:
2215 1674 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
AvaI C'yCGr_G
Cuts at: 1870 1943 3049 3692 1870
Size: 73 1106 643 4545
Fragments arranged by size:
4545 1106 643 73
BamHI G'GATC_C
Cuts at: 3697 4064 3697
Size: 367 6000
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3688 5143 618
Size: 325 217 627 1901 1455 1842
Fragments arranged by size:
1901 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3686 1391
Size: 2295 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2458 3655 928
Size: 971 559 1197 3640
Fragments arranged by size:
3640 1197 971 559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4558 4592 1903
Size: 34 2621 34 3678
Fragments arranged by size:
3678 2621 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3306 4259 5469 2397
Size: 909 953 1210 3295
Fragments arranged by size:
3295 1210 953 909
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6367
BfrBI ATG'CAT
Cuts at: 3064 3064
Size: 6367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4978 136
Size: 107 122 71 631 605 3306 1525
Fragments arranged by size:
3306 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6362 6362
Size: 6367
BlpI GC'TnA_GC
Cuts at: 3784 3784
Size: 6367
Bme1580I G_kGCm'C
Cuts at: 1790 3337 4428 5584 1790
Size: 1547 1091 1156 2573
Fragments arranged by size:
2573 1547 1156 1091
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 5473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2045 2262 2347
Size: 416 53 83 399 24 628 217 85
Cuts at: 2347 2922 4098 5056 442
Size: 575 1176 958 1753
Fragments arranged by size:
1753 1176 958 628 575 416 399 217
85 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2339 2590 2771 2339
Size: 251 181 5935
Fragments arranged by size:
5935 251 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 3018 3186 3438 3522 5027 1239
Size: 1001 778 168 252 84 1505 2579
Fragments arranged by size:
2579 1505 1001 778 252 168 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4492 5357 5655 4492
Size: 865 298 5204
Fragments arranged by size:
5204 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5030 882
Size: 617 3531 2219
Fragments arranged by size:
3531 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2497 3546 4158 493
Size: 711 1293 1049 612 2702
Fragments arranged by size:
2702 1293 1049 711 612
BsaBI GATnn'nnATC
Cuts at: 2115 6361 2115
Size: 4246 2121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4174 4556 5191 275
Size: 53 83 186 936 2641 382 635 1451
Fragments arranged by size:
2641 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4800 5198 5472 5619 6030 4800
Size: 398 274 147 411 5137
Fragments arranged by size:
5137 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6142
Fragments arranged by size:
6142 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2331 1186
Size: 994 151 5222
Fragments arranged by size:
5222 994 151
BseYI C'CCAG_C
Cuts at: 2878 5570 6066 2878
Size: 2692 496 3179
Fragments arranged by size:
3179 2692 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5794
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3817 4578 5606 1540
Size: 275 186 1816 761 1028 2301
Fragments arranged by size:
2301 1816 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4428 4513 5584 4428
Size: 85 1071 5211
Fragments arranged by size:
5211 1071 85
BsmI GAATG_Cn'
Cuts at: 3524 3878 3971 3524
Size: 354 93 5920
Fragments arranged by size:
5920 354 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3337 3686 4428 4513 5584 1391
Size: 399 1547 349 742 85 1071 2174
Fragments arranged by size:
2174 1547 1071 742 399 349 85
BspEI T'CCGG_A
Cuts at: 6030 6030
Size: 6367
BspHI T'CATG_A
Cuts at: 2062 2524 3568 4256 5986 2062
Size: 462 1044 688 1730 2443
Fragments arranged by size:
2443 1730 1044 688 462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3722 844
Size: 2878 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4254 1394
Size: 429 2431 3507
Fragments arranged by size:
3507 2431 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2459 3617 4862 5036 65
Size: 1183 1211 1158 1245 174 1396
Fragments arranged by size:
1396 1245 1211 1183 1158 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5011 5198 1700
Size: 110 3201 187 2869
Fragments arranged by size:
3201 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6367
BssSI C'ACGA_G
Cuts at: 4427 5439 4427
Size: 1012 5355
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6367
BstBI TT'CG_AA
Cuts at: 3646 5171 3646
Size: 1525 4842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6367
BstYI r'GATC_y
Cuts at: 1972 3697 4064 4449 4466 5918 6004 6016 6362
Size: 1725 367 385 17 1452 86 12 346
Cuts at: 6362 1972
Size: 1977
Fragments arranged by size:
1977 1725 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2644 2644
Size: 6367
BtgI C'CryG_G
Cuts at: 513 1065 2004 2026 513
Size: 552 939 22 4854
Fragments arranged by size:
4854 939 552 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5628
Fragments arranged by size:
5628 683 56
BtsI GCAGTG_nn'
Cuts at: 3883 4677 4697 3883
Size: 794 20 5553
Fragments arranged by size:
5553 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3511 3546 529
Size: 35 2947 35 3350
Fragments arranged by size:
3350 2947 35 35
DraI TTT'AAA
Cuts at: 3671 3671
Size: 6367
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5374 809
Size: 507 4058 1802
Fragments arranged by size:
4058 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 3814 4703
Size: 54 1425 50 275 18 408 1576 889
Cuts at: 4703 6332 8
Size: 1629 43
Fragments arranged by size:
1629 1576 1425 889 408 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3814 1537
Size: 275 2002 4090
Fragments arranged by size:
4090 2002 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 3246 3582 4297 1353
Size: 936 957 336 715 3423
Fragments arranged by size:
3423 957 936 715 336
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4954 5328 5474 387
Size: 1064 138 3365 374 146 1280
Fragments arranged by size:
3365 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2351 2452 2605 2705 3018 3186
Size: 393 608 111 101 153 100 313 168
Cuts at: 3186 3270 3438 3442 3522 4430 5027 5814 6082
Size: 84 168 4 80 908 597 787 268
Cuts at: 6082 1239
Size: 1524
Fragments arranged by size:
1524 908 787 608 597 393 313 268
168 168 153 111 101 100 84 80
4
EcoO109I rG'GnC_Cy
Cuts at: 2047 2047
Size: 6367
EcoRI G'AATT_C
Cuts at: 3564 3676 4054 3564
Size: 112 378 5877
Fragments arranged by size:
5877 378 112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2570 2570
Size: 6367
FspI TGC'GCA
Cuts at: 4873 4873
Size: 6367
HaeII r_GCGC'y
Cuts at: 1678 5514 1678
Size: 3836 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 5008 5040 5082
Size: 32 135 32 669 32 2735 32 42
Cuts at: 5082 5114 1373
Size: 32 2626
Fragments arranged by size:
2735 2626 669 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3711 3957 669
Size: 988 281 1773 246 3079
Fragments arranged by size:
3079 1773 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3621 748
Size: 2873 3494
HpaI GTT'AAC
Cuts at: 3957 3957
Size: 6367
KpnI G_GTAC'C
Cuts at: 3692 3692
Size: 6367
MfeI C'AATT_G
Cuts at: 2408 3966 2408
Size: 1558 4809
MluI A'CGCG_T
Cuts at: 6243 6243
Size: 6367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 2988 5481 5665 1305
Size: 960 723 2493 184 2007
Fragments arranged by size:
2493 2007 960 723 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 10
Size: 54 1425 343 408 4137
Fragments arranged by size:
4137 1425 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2677 2929 4325 4684 4843 518
Size: 648 1511 252 1396 359 159 2042
Fragments arranged by size:
2042 1511 1396 648 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 2012 3783 4460 5608 5853 1606
Size: 406 1771 677 1148 245 2120
Fragments arranged by size:
2120 1771 1148 677 406 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2026 513
Size: 552 939 22 4854
Fragments arranged by size:
4854 939 552 22
NdeI CA'TA_TG
Cuts at: 387 2730 2926 387
Size: 2343 196 3828
Fragments arranged by size:
3828 2343 196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6367
NotI GC'GGCC_GC
Cuts at: 3814 3814
Size: 6367
NsiI A_TGCA'T
Cuts at: 3066 3066
Size: 6367
NspI r_CATG'y
Cuts at: 1132 1229 2902 3068 3322 3406 3725 5270 1132
Size: 97 1673 166 254 84 319 1545 2229
Fragments arranged by size:
2229 1673 1545 319 254 166 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6367
PciI A'CATG_T
Cuts at: 5266 5266
Size: 6367
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6367
PmeI GTTT'AAAC
Cuts at: 3671 3671
Size: 6367
PmlI CAC'GTG
Cuts at: 2497 3546 2497
Size: 1049 5318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 5949 5981 4436
Size: 32 1481 32 4822
Fragments arranged by size:
4822 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 2047
Size: 6367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6367
PsiI TTA'TAA
Cuts at: 3937 3937
Size: 6367
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2494 2526 2494
Size: 32 6335
PstI C_TGCA'G
Cuts at: 830 3719 830
Size: 2889 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6367
PvuII CAG'CTG
Cuts at: 2012 2012
Size: 6367
SalI G'TCGA_C
Cuts at: 1936 3709 1936
Size: 1773 4594
SanDI GG'GwC_CC
Cuts at: 2047 2047
Size: 6367
SbfI CC_TGCA'GG
Cuts at: 3719 3719
Size: 6367
ScaI AGT'ACT
Cuts at: 3047 4615 6327 3047
Size: 1568 1712 3087
Fragments arranged by size:
3087 1712 1568
SfcI C'TryA_G
Cuts at: 826 1045 2203 2869 3715 4850 5531 5722 826
Size: 219 1158 666 846 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 846 681 666 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6367
SmaI CCC'GGG
Cuts at: 1872 3694 1872
Size: 1822 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 4471 5372 5634 820
Size: 197 926 543 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 4158 493
Size: 3665 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6367
SphI G_CATG'C
Cuts at: 1132 3725 1132
Size: 2593 3774
SspI AAT'ATT
Cuts at: 5 52 2638 4291 5
Size: 47 2586 1653 2081
Fragments arranged by size:
2586 2081 1653 47
StuI AGG'CCT
Cuts at: 1517 3260 3455 1517
Size: 1743 195 4429
Fragments arranged by size:
4429 1743 195
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2326 3806 513
Size: 552 939 22 300 1480 3074
Fragments arranged by size:
3074 1480 939 552 300 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2174 3401 4394 4411 4564 4749 5194
Size: 1255 594 1227 993 17 153 185 445
Cuts at: 5194 325
Size: 1498
Fragments arranged by size:
1498 1255 1227 993 594 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2510 3045 4613
Size: 274 80 33 51 1443 533 535 1568
Cuts at: 4613 6325 96
Size: 1712 138
Fragments arranged by size:
1712 1568 1443 535 533 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 6327 6359 2076
Size: 32 4219 32 2084
Fragments arranged by size:
4219 2084 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6367
XbaI T'CTAG_A
Cuts at: 3703 3703
Size: 6367
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6367
XmaI C'CCGG_G
Cuts at: 1870 3692 1870
Size: 1822 4545
XmnI GAAnn'nnTTC
Cuts at: 2951 3035 3119 3539 3660 4496 2951
Size: 84 84 420 121 836 4822
Fragments arranged by size:
4822 836 420 121 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4175 276
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PspOMI PspXI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI