Restriction map for
pFN21AB7861
(Circular) (Six-base) MAPSORT of: pFN21AB7861.seq Check: 6846 from: 1 to: 6367
pFN21AB7861 6367 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3107 3107
Size: 6367
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4177 278
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3710 1937
Size: 1773 4594
Acc65I G'GTAC_C
Cuts at: 2116 3688 2116
Size: 1572 4795
AclI AA'CG_TT
Cuts at: 1563 4494 4867 1563
Size: 2931 373 3063
Fragments arranged by size:
3063 2931 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2482 2650 2938 3106 3358 3442 3526
Size: 474 376 168 288 168 252 84 84
Cuts at: 3526 4430 5814 6082 1632
Size: 904 1384 268 1917
Fragments arranged by size:
1917 1384 904 474 376 288 268 252
168 168 84 84
AfeI AGC'GCT
Cuts at: 2346 3035 3203 2346
Size: 689 168 5510
Fragments arranged by size:
5510 689 168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6170
AflIII A'CryG_T
Cuts at: 1203 3180 5266 6243 1203
Size: 1977 2086 977 1327
Fragments arranged by size:
2086 1977 1327 977
AgeI A'CCGG_T
Cuts at: 2928 5198 2928
Size: 2270 4097
AhdI GACnn_n'nnGTC
Cuts at: 5096 5096
Size: 6367
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2355 1184
Size: 1171 5196
AlwNI CAG_nnn'CTG
Cuts at: 4110 5682 4110
Size: 1572 4795
ApaI G_GGCC'C
Cuts at: 2518 3594 2518
Size: 1076 5291
ApaLI G'TGCA_C
Cuts at: 2593 4424 5580 2593
Size: 1831 1156 3380
Fragments arranged by size:
3380 1831 1156
ApoI r'AATT_y
Cuts at: 1493 3676 3902 4054 6163 1493
Size: 2183 226 152 2109 1697
Fragments arranged by size:
2183 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4921 160
Size: 872 3889 1606
Fragments arranged by size:
3889 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
AvaI C'yCGr_G
Cuts at: 1870 1943 2307 2406 2545 3356 3692 1870
Size: 73 364 99 139 811 336 4545
Fragments arranged by size:
4545 811 364 336 139 99 73
BamHI G'GATC_C
Cuts at: 2292 3697 4064 2292
Size: 1405 367 4595
Fragments arranged by size:
4595 1405 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2116 2210 2224 2867 3239
Size: 325 217 627 329 94 14 643 372
Cuts at: 3239 3251 3688 5143 618
Size: 12 437 1455 1842
Fragments arranged by size:
1842 1455 643 627 437 372 329 325
217 94 14 12
BanII G_rGCy'C
Cuts at: 1391 2163 2206 2278 2518 2552 2738 2823 3302
Size: 772 43 72 240 34 186 85 479
Cuts at: 3302 3594 3640 3686 1391
Size: 292 46 46 4072
Fragments arranged by size:
4072 772 479 292 240 186 85 72
46 46 43 34
BbeI G_GCGC'C
Cuts at: 2871 3255 2871
Size: 384 5983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4558 4592 1903
Size: 34 2621 34 3678
Fragments arranged by size:
3678 2621 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4259 5469 4259
Size: 1210 5157
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4978 136
Size: 107 122 71 631 605 3306 1525
Fragments arranged by size:
3306 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6362 6362
Size: 6367
BlpI GC'TnA_GC
Cuts at: 2769 3784 2769
Size: 1015 5352
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2518 2597 3242 3272 3356 3594 4428
Size: 437 291 79 645 30 84 238 834
Cuts at: 4428 5584 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 834 645 437 291 238 84
79 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6367
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2495 2802 3342
Size: 416 53 83 399 24 1078 307 540
Cuts at: 3342 4098 5056 442
Size: 756 958 1753
Fragments arranged by size:
1753 1078 958 756 540 416 399 307
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 6367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2536 5027 1239
Size: 1297 2491 2579
Fragments arranged by size:
2579 2491 1297
Bpu10I CC'TnA_GC
Cuts at: 2737 2737
Size: 6367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2190 2505 4492 5357 5655 2190
Size: 315 1987 865 298 2902
Fragments arranged by size:
2902 1987 865 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2319 5030 882
Size: 617 820 2711 2219
Fragments arranged by size:
2711 2219 820 617
BsaAI yAC'GTr
Cuts at: 493 1204 3181 4158 493
Size: 711 1977 977 2702
Fragments arranged by size:
2702 1977 977 711
BsaBI GATnn'nnATC
Cuts at: 6361 6361
Size: 6367
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2697 2868 3252 4174
Size: 53 83 186 936 1164 171 384 922
Cuts at: 4174 4556 5191 275
Size: 382 635 1451
Fragments arranged by size:
1451 1164 936 922 635 384 382 186
171 83 53
BsaWI w'CCGG_w
Cuts at: 2928 3164 4800 5198 5472 5619 6030 2928
Size: 236 1636 398 274 147 411 3265
Fragments arranged by size:
3265 1636 411 398 274 236 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6142
Fragments arranged by size:
6142 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2426 2870 2873 2900 1186
Size: 1153 87 444 3 27 4653
Fragments arranged by size:
4653 1153 444 87 27 3
BseYI C'CCAG_C
Cuts at: 2104 2220 3616 5570 6066 2104
Size: 116 1396 1954 496 2405
Fragments arranged by size:
2405 1954 1396 496 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2645 3001 3545 1163
Size: 573 393 516 356 544 3985
Fragments arranged by size:
3985 573 544 516 393 356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 3043 3190 3817 4578 5606
Size: 275 186 730 312 147 627 761 1028
Cuts at: 5606 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 730 627 312 275 186
147
BsiHKAI G_wGCw'C
Cuts at: 2597 2738 3302 4428 4513 5584 2597
Size: 141 564 1126 85 1071 3380
Fragments arranged by size:
3380 1126 1071 564 141 85
BsmI GAATG_Cn'
Cuts at: 3878 3971 3878
Size: 93 6274
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2206 2227 2278 2518 2552 2597
Size: 399 373 43 21 51 240 34 45
Cuts at: 2597 2738 2823 3242 3272 3302 3356 3594 3640
Size: 141 85 419 30 30 54 238 46
Cuts at: 3640 3686 4428 4513 5584 1391
Size: 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 399 373 240 238
141 85 85 54 51 46 46 45
43 34 30 30 21
BspEI T'CCGG_A
Cuts at: 6030 6030
Size: 6367
BspHI T'CATG_A
Cuts at: 4256 5986 4256
Size: 1730 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 3567 3722 844
Size: 2263 460 155 3489
Fragments arranged by size:
3489 2263 460 155
BsrBI CCG'CTC
Cuts at: 1394 1823 3216 3659 4254 1394
Size: 429 1393 443 595 3507
Fragments arranged by size:
3507 1393 595 443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4862 5036 65
Size: 1183 3614 174 1396
Fragments arranged by size:
3614 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 2556 2743 2764 2928 2982 3546
Size: 110 518 228 187 21 164 54 564
Cuts at: 3546 3564 5011 5198 1700
Size: 18 1447 187 2869
Fragments arranged by size:
2869 1447 564 518 228 187 187 164
110 54 21 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6367
BssHII G'CGCG_C
Cuts at: 1925 2709 1925
Size: 784 5583
BssSI C'ACGA_G
Cuts at: 3569 4427 5439 3569
Size: 858 1012 4497
Fragments arranged by size:
4497 1012 858
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2623 3024 794
Size: 1829 401 4137
Fragments arranged by size:
4137 1829 401
BstBI TT'CG_AA
Cuts at: 5171 5171
Size: 6367
BstEII G'GTnAC_C
Cuts at: 1184 3226 1184
Size: 2042 4325
BstXI CCAn_nnnn'nTGG
Cuts at: 3601 3601
Size: 6367
BstYI r'GATC_y
Cuts at: 1972 2292 3576 3697 4064 4449 4466 5918 6004
Size: 320 1284 121 367 385 17 1452 86
Cuts at: 6004 6016 6362 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1284 385 367 346 320 121
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2637 2814 3499 513
Size: 552 1572 177 685 3381
Fragments arranged by size:
3381 1572 685 552 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2172 3379 487
Size: 683 56 946 1207 3475
Fragments arranged by size:
3475 1207 946 683 56
BtsI GCAGTG_nn'
Cuts at: 2141 2352 2711 2945 3433 3883 4677 4697 2141
Size: 211 359 234 488 450 794 20 3811
Fragments arranged by size:
3811 794 488 450 359 234 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6332
DraI TTT'AAA
Cuts at: 3671 3671
Size: 6367
DraIII CAC_nnn'GTG
Cuts at: 2974 2974
Size: 6367
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5374 809
Size: 507 4058 1802
Fragments arranged by size:
4058 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2728 2745 3040
Size: 54 1425 50 275 18 898 17 295
Cuts at: 3040 3603 3642 3814 4703 6332 8
Size: 563 39 172 889 1629 43
Fragments arranged by size:
1629 1425 898 889 563 295 275 172
54 50 43 39 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2728 3040 3814 1537
Size: 275 916 312 774 4090
Fragments arranged by size:
4090 916 774 312 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 2264 2915 3110 4297 1353
Size: 807 104 651 195 1187 3423
Fragments arranged by size:
3423 1187 807 651 195 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2034 2286 2787 4954 5328 5474
Size: 1064 138 445 252 501 2167 374 146
Cuts at: 5474 387
Size: 1280
Fragments arranged by size:
2167 1280 1064 501 445 374 252 146
138
EcoICRI GAG'CTC
Cuts at: 2736 3300 2736
Size: 564 5803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2482 2536 2650 2938 3106 3358
Size: 393 474 376 54 114 288 168 252
Cuts at: 3358 3442 3526 4430 5027 5814 6082 1239
Size: 84 84 904 597 787 268 1524
Fragments arranged by size:
1524 904 787 597 474 393 376 288
268 252 168 114 84 84 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2500 2500
Size: 6367
EcoO109I rG'GnC_Cy
Cuts at: 2515 2890 3598 2515
Size: 375 708 5284
Fragments arranged by size:
5284 708 375
EcoRI G'AATT_C
Cuts at: 3676 4054 3676
Size: 378 5989
FseI GG_CCGG'CC
Cuts at: 2747 2747
Size: 6367
FspI TGC'GCA
Cuts at: 4873 4873
Size: 6367
HaeII r_GCGC'y
Cuts at: 1678 2348 2871 3037 3205 3255 3308 3553 5514
Size: 670 523 166 168 50 53 245 1961
Cuts at: 5514 1678
Size: 2531
Fragments arranged by size:
2531 1961 670 523 245 168 166 53
50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 5008 5040 5082
Size: 32 135 32 599 32 2805 32 42
Cuts at: 5082 5114 1373
Size: 32 2626
Fragments arranged by size:
2805 2626 599 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3711 3957 669
Size: 988 281 1773 246 3079
Fragments arranged by size:
3079 1773 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6367
HpaI GTT'AAC
Cuts at: 3957 3957
Size: 6367
KasI G'GCGC_C
Cuts at: 2867 3251 2867
Size: 384 5983
KpnI G_GTAC'C
Cuts at: 2120 3692 2120
Size: 1572 4795
MfeI C'AATT_G
Cuts at: 3966 3966
Size: 6367
MluI A'CGCG_T
Cuts at: 6243 6243
Size: 6367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3411 3579 5481 5665 1305
Size: 2106 168 1902 184 2007
Fragments arranged by size:
2106 2007 1902 184 168
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3605 3644 10
Size: 54 1425 343 1773 39 2733
Fragments arranged by size:
2733 1773 1425 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2153 3611 4325 4684 4843 518
Size: 648 987 1458 714 359 159 2042
Fragments arranged by size:
2042 1458 987 714 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2958 3192 3783 4460 5608 5853 1606
Size: 1352 234 591 677 1148 245 2120
Fragments arranged by size:
2120 1352 1148 677 591 245 234
NaeI GCC'GGC
Cuts at: 2558 2745 2766 3548 2558
Size: 187 21 782 5377
Fragments arranged by size:
5377 782 187 21
NarI GG'CG_CC
Cuts at: 2868 3252 2868
Size: 384 5983
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5815
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6367
NgoMIV G'CCGG_C
Cuts at: 2556 2743 2764 3546 2556
Size: 187 21 782 5377
Fragments arranged by size:
5377 782 187 21
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6367
NotI GC'GGCC_GC
Cuts at: 3814 3814
Size: 6367
NspI r_CATG'y
Cuts at: 1132 1229 3725 5270 1132
Size: 97 2496 1545 2229
Fragments arranged by size:
2496 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6367
PciI A'CATG_T
Cuts at: 5266 5266
Size: 6367
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2072 1108
Size: 964 5403
PfoI T'CCnGG_A
Cuts at: 2028 2838 2028
Size: 810 5557
PmeI GTTT'AAAC
Cuts at: 3671 3671
Size: 6367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 5949 5981 4436
Size: 32 1481 32 4822
Fragments arranged by size:
4822 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2808 1613
Size: 1195 5172
PsiI TTA'TAA
Cuts at: 3937 3937
Size: 6367
PspOMI G'GGCC_C
Cuts at: 2514 3590 2514
Size: 1076 5291
PstI C_TGCA'G
Cuts at: 830 2014 2344 2466 3719 830
Size: 1184 330 122 1253 3478
Fragments arranged by size:
3478 1253 1184 330 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6367
PvuII CAG'CTG
Cuts at: 2958 2958
Size: 6367
SacI G_AGCT'C
Cuts at: 2738 3302 2738
Size: 564 5803
SalI G'TCGA_C
Cuts at: 1936 3709 1936
Size: 1773 4594
SapI GCTCTTCn'nnn_
Cuts at: 2160 2915 3110 2160
Size: 755 195 5417
Fragments arranged by size:
5417 755 195
SbfI CC_TGCA'GG
Cuts at: 3719 3719
Size: 6367
ScaI AGT'ACT
Cuts at: 4615 6327 4615
Size: 1712 4655
SexAI A'CCwGG_T
Cuts at: 2970 2970
Size: 6367
SfcI C'TryA_G
Cuts at: 826 1045 2010 2340 2449 2462 3715 4850 5531
Size: 219 965 330 109 13 1253 1135 681
Cuts at: 5531 5722 826
Size: 191 1471
Fragments arranged by size:
1471 1253 1135 965 681 330 219 191
109 13
SfoI GGC'GCC
Cuts at: 2869 3253 2869
Size: 384 5983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
SgrAI Cr'CCGG_yG
Cuts at: 1700 2982 1700
Size: 1282 5085
SmaI CCC'GGG
Cuts at: 1872 2408 3694 1872
Size: 536 1286 4545
Fragments arranged by size:
4545 1286 536
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2205 2520 4471 5372 5634 820
Size: 197 926 262 315 1951 901 262 1553
Fragments arranged by size:
1951 1553 926 901 315 262 262 197
SnaBI TAC'GTA
Cuts at: 493 4158 493
Size: 3665 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6367
SphI G_CATG'C
Cuts at: 1132 3725 1132
Size: 2593 3774
SspI AAT'ATT
Cuts at: 5 52 4291 5
Size: 47 4239 2081
Fragments arranged by size:
4239 2081 47
StuI AGG'CCT
Cuts at: 1517 2506 2543 1517
Size: 989 37 5341
Fragments arranged by size:
5341 989 37
StyI C'CwwG_G
Cuts at: 513 1065 2647 3594 3806 513
Size: 552 1582 947 212 3074
Fragments arranged by size:
3074 1582 947 552 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 3245 3257 3329 3341 3509 4394
Size: 1255 471 1194 12 72 12 168 885
Cuts at: 4394 4411 4564 4749 5194 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1194 885 471 445 185 168
153 72 17 12 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4613 6325 96
Size: 274 80 33 51 1443 2636 1712 138
Fragments arranged by size:
2636 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6327 6359 6327
Size: 32 6335
Tth111I GACn'n_nGTC
Cuts at: 1316 3224 1316
Size: 1908 4459
XbaI T'CTAG_A
Cuts at: 3703 3703
Size: 6367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3613 3613
Size: 6367
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6367
XmaI C'CCGG_G
Cuts at: 1870 2406 3692 1870
Size: 536 1286 4545
Fragments arranged by size:
4545 1286 536
XmnI GAAnn'nnTTC
Cuts at: 2663 3035 3119 4496 2663
Size: 372 84 1377 4534
Fragments arranged by size:
4534 1377 372 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4175 276
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FalI FspAI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI