(Circular) (Six-base) MAPSORT of: pFN21AB7861.seq Check: 6846 from: 1 to: 6367 pFN21AB7861 6367 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3107 3107 Size: 6367 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4177 278 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3710 1937 Size: 1773 4594 Acc65I G'GTAC_C Cuts at: 2116 3688 2116 Size: 1572 4795 AclI AA'CG_TT Cuts at: 1563 4494 4867 1563 Size: 2931 373 3063 Fragments arranged by size: 3063 2931 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2482 2650 2938 3106 3358 3442 3526 Size: 474 376 168 288 168 252 84 84 Cuts at: 3526 4430 5814 6082 1632 Size: 904 1384 268 1917 Fragments arranged by size: 1917 1384 904 474 376 288 268 252 168 168 84 84 AfeI AGC'GCT Cuts at: 2346 3035 3203 2346 Size: 689 168 5510 Fragments arranged by size: 5510 689 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6170 AflIII A'CryG_T Cuts at: 1203 3180 5266 6243 1203 Size: 1977 2086 977 1327 Fragments arranged by size: 2086 1977 1327 977 AgeI A'CCGG_T Cuts at: 2928 5198 2928 Size: 2270 4097 AhdI GACnn_n'nnGTC Cuts at: 5096 5096 Size: 6367 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2355 1184 Size: 1171 5196 AlwNI CAG_nnn'CTG Cuts at: 4110 5682 4110 Size: 1572 4795 ApaI G_GGCC'C Cuts at: 2518 3594 2518 Size: 1076 5291 ApaLI G'TGCA_C Cuts at: 2593 4424 5580 2593 Size: 1831 1156 3380 Fragments arranged by size: 3380 1831 1156 ApoI r'AATT_y Cuts at: 1493 3676 3902 4054 6163 1493 Size: 2183 226 152 2109 1697 Fragments arranged by size: 2183 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4921 160 Size: 872 3889 1606 Fragments arranged by size: 3889 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 AvaI C'yCGr_G Cuts at: 1870 1943 2307 2406 2545 3356 3692 1870 Size: 73 364 99 139 811 336 4545 Fragments arranged by size: 4545 811 364 336 139 99 73 BamHI G'GATC_C Cuts at: 2292 3697 4064 2292 Size: 1405 367 4595 Fragments arranged by size: 4595 1405 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2116 2210 2224 2867 3239 Size: 325 217 627 329 94 14 643 372 Cuts at: 3239 3251 3688 5143 618 Size: 12 437 1455 1842 Fragments arranged by size: 1842 1455 643 627 437 372 329 325 217 94 14 12 BanII G_rGCy'C Cuts at: 1391 2163 2206 2278 2518 2552 2738 2823 3302 Size: 772 43 72 240 34 186 85 479 Cuts at: 3302 3594 3640 3686 1391 Size: 292 46 46 4072 Fragments arranged by size: 4072 772 479 292 240 186 85 72 46 46 43 34 BbeI G_GCGC'C Cuts at: 2871 3255 2871 Size: 384 5983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5396 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4558 4592 1903 Size: 34 2621 34 3678 Fragments arranged by size: 3678 2621 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4259 5469 4259 Size: 1210 5157 BclI T'GATC_A Cuts at: 1548 1548 Size: 6367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4978 136 Size: 107 122 71 631 605 3306 1525 Fragments arranged by size: 3306 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6362 6362 Size: 6367 BlpI GC'TnA_GC Cuts at: 2769 3784 2769 Size: 1015 5352 Bme1580I G_kGCm'C Cuts at: 1790 2227 2518 2597 3242 3272 3356 3594 4428 Size: 437 291 79 645 30 84 238 834 Cuts at: 4428 5584 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 834 645 437 291 238 84 79 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6367 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 2802 3342 Size: 416 53 83 399 24 1078 307 540 Cuts at: 3342 4098 5056 442 Size: 756 958 1753 Fragments arranged by size: 1753 1078 958 756 540 416 399 307 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6367 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 6367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2536 5027 1239 Size: 1297 2491 2579 Fragments arranged by size: 2579 2491 1297 Bpu10I CC'TnA_GC Cuts at: 2737 2737 Size: 6367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2190 2505 4492 5357 5655 2190 Size: 315 1987 865 298 2902 Fragments arranged by size: 2902 1987 865 315 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2319 5030 882 Size: 617 820 2711 2219 Fragments arranged by size: 2711 2219 820 617 BsaAI yAC'GTr Cuts at: 493 1204 3181 4158 493 Size: 711 1977 977 2702 Fragments arranged by size: 2702 1977 977 711 BsaBI GATnn'nnATC Cuts at: 6361 6361 Size: 6367 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2697 2868 3252 4174 Size: 53 83 186 936 1164 171 384 922 Cuts at: 4174 4556 5191 275 Size: 382 635 1451 Fragments arranged by size: 1451 1164 936 922 635 384 382 186 171 83 53 BsaWI w'CCGG_w Cuts at: 2928 3164 4800 5198 5472 5619 6030 2928 Size: 236 1636 398 274 147 411 3265 Fragments arranged by size: 3265 1636 411 398 274 236 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6142 Fragments arranged by size: 6142 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2426 2870 2873 2900 1186 Size: 1153 87 444 3 27 4653 Fragments arranged by size: 4653 1153 444 87 27 3 BseYI C'CCAG_C Cuts at: 2104 2220 3616 5570 6066 2104 Size: 116 1396 1954 496 2405 Fragments arranged by size: 2405 1954 1396 496 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2645 3001 3545 1163 Size: 573 393 516 356 544 3985 Fragments arranged by size: 3985 573 544 516 393 356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 3043 3190 3817 4578 5606 Size: 275 186 730 312 147 627 761 1028 Cuts at: 5606 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 730 627 312 275 186 147 BsiHKAI G_wGCw'C Cuts at: 2597 2738 3302 4428 4513 5584 2597 Size: 141 564 1126 85 1071 3380 Fragments arranged by size: 3380 1126 1071 564 141 85 BsmI GAATG_Cn' Cuts at: 3878 3971 3878 Size: 93 6274 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2206 2227 2278 2518 2552 2597 Size: 399 373 43 21 51 240 34 45 Cuts at: 2597 2738 2823 3242 3272 3302 3356 3594 3640 Size: 141 85 419 30 30 54 238 46 Cuts at: 3640 3686 4428 4513 5584 1391 Size: 46 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 419 399 373 240 238 141 85 85 54 51 46 46 45 43 34 30 30 21 BspEI T'CCGG_A Cuts at: 6030 6030 Size: 6367 BspHI T'CATG_A Cuts at: 4256 5986 4256 Size: 1730 4637 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3107 3567 3722 844 Size: 2263 460 155 3489 Fragments arranged by size: 3489 2263 460 155 BsrBI CCG'CTC Cuts at: 1394 1823 3216 3659 4254 1394 Size: 429 1393 443 595 3507 Fragments arranged by size: 3507 1393 595 443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4862 5036 65 Size: 1183 3614 174 1396 Fragments arranged by size: 3614 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2328 2556 2743 2764 2928 2982 3546 Size: 110 518 228 187 21 164 54 564 Cuts at: 3546 3564 5011 5198 1700 Size: 18 1447 187 2869 Fragments arranged by size: 2869 1447 564 518 228 187 187 164 110 54 21 18 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6367 BssHII G'CGCG_C Cuts at: 1925 2709 1925 Size: 784 5583 BssSI C'ACGA_G Cuts at: 3569 4427 5439 3569 Size: 858 1012 4497 Fragments arranged by size: 4497 1012 858 BstAPI GCAn_nnn'nTGC Cuts at: 794 2623 3024 794 Size: 1829 401 4137 Fragments arranged by size: 4137 1829 401 BstBI TT'CG_AA Cuts at: 5171 5171 Size: 6367 BstEII G'GTnAC_C Cuts at: 1184 3226 1184 Size: 2042 4325 BstXI CCAn_nnnn'nTGG Cuts at: 3601 3601 Size: 6367 BstYI r'GATC_y Cuts at: 1972 2292 3576 3697 4064 4449 4466 5918 6004 Size: 320 1284 121 367 385 17 1452 86 Cuts at: 6004 6016 6362 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1284 385 367 346 320 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2637 2814 3499 513 Size: 552 1572 177 685 3381 Fragments arranged by size: 3381 1572 685 552 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2172 3379 487 Size: 683 56 946 1207 3475 Fragments arranged by size: 3475 1207 946 683 56 BtsI GCAGTG_nn' Cuts at: 2141 2352 2711 2945 3433 3883 4677 4697 2141 Size: 211 359 234 488 450 794 20 3811 Fragments arranged by size: 3811 794 488 450 359 234 211 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6332 DraI TTT'AAA Cuts at: 3671 3671 Size: 6367 DraIII CAC_nnn'GTG Cuts at: 2974 2974 Size: 6367 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5374 809 Size: 507 4058 1802 Fragments arranged by size: 4058 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2728 2745 3040 Size: 54 1425 50 275 18 898 17 295 Cuts at: 3040 3603 3642 3814 4703 6332 8 Size: 563 39 172 889 1629 43 Fragments arranged by size: 1629 1425 898 889 563 295 275 172 54 50 43 39 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2728 3040 3814 1537 Size: 275 916 312 774 4090 Fragments arranged by size: 4090 916 774 312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 2264 2915 3110 4297 1353 Size: 807 104 651 195 1187 3423 Fragments arranged by size: 3423 1187 807 651 195 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2034 2286 2787 4954 5328 5474 Size: 1064 138 445 252 501 2167 374 146 Cuts at: 5474 387 Size: 1280 Fragments arranged by size: 2167 1280 1064 501 445 374 252 146 138 EcoICRI GAG'CTC Cuts at: 2736 3300 2736 Size: 564 5803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2482 2536 2650 2938 3106 3358 Size: 393 474 376 54 114 288 168 252 Cuts at: 3358 3442 3526 4430 5027 5814 6082 1239 Size: 84 84 904 597 787 268 1524 Fragments arranged by size: 1524 904 787 597 474 393 376 288 268 252 168 114 84 84 54 EcoNI CCTnn'n_nnAGG Cuts at: 2500 2500 Size: 6367 EcoO109I rG'GnC_Cy Cuts at: 2515 2890 3598 2515 Size: 375 708 5284 Fragments arranged by size: 5284 708 375 EcoRI G'AATT_C Cuts at: 3676 4054 3676 Size: 378 5989 FseI GG_CCGG'CC Cuts at: 2747 2747 Size: 6367 FspI TGC'GCA Cuts at: 4873 4873 Size: 6367 HaeII r_GCGC'y Cuts at: 1678 2348 2871 3037 3205 3255 3308 3553 5514 Size: 670 523 166 168 50 53 245 1961 Cuts at: 5514 1678 Size: 2531 Fragments arranged by size: 2531 1961 670 523 245 168 166 53 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 5008 5040 5082 Size: 32 135 32 599 32 2805 32 42 Cuts at: 5082 5114 1373 Size: 32 2626 Fragments arranged by size: 2805 2626 599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3711 3957 669 Size: 988 281 1773 246 3079 Fragments arranged by size: 3079 1773 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6367 HpaI GTT'AAC Cuts at: 3957 3957 Size: 6367 KasI G'GCGC_C Cuts at: 2867 3251 2867 Size: 384 5983 KpnI G_GTAC'C Cuts at: 2120 3692 2120 Size: 1572 4795 MfeI C'AATT_G Cuts at: 3966 3966 Size: 6367 MluI A'CGCG_T Cuts at: 6243 6243 Size: 6367 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3411 3579 5481 5665 1305 Size: 2106 168 1902 184 2007 Fragments arranged by size: 2106 2007 1902 184 168 MscI TGG'CCA Cuts at: 10 64 1489 1832 3605 3644 10 Size: 54 1425 343 1773 39 2733 Fragments arranged by size: 2733 1773 1425 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2153 3611 4325 4684 4843 518 Size: 648 987 1458 714 359 159 2042 Fragments arranged by size: 2042 1458 987 714 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2958 3192 3783 4460 5608 5853 1606 Size: 1352 234 591 677 1148 245 2120 Fragments arranged by size: 2120 1352 1148 677 591 245 234 NaeI GCC'GGC Cuts at: 2558 2745 2766 3548 2558 Size: 187 21 782 5377 Fragments arranged by size: 5377 782 187 21 NarI GG'CG_CC Cuts at: 2868 3252 2868 Size: 384 5983 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5815 NdeI CA'TA_TG Cuts at: 387 387 Size: 6367 NgoMIV G'CCGG_C Cuts at: 2556 2743 2764 3546 2556 Size: 187 21 782 5377 Fragments arranged by size: 5377 782 187 21 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6367 NotI GC'GGCC_GC Cuts at: 3814 3814 Size: 6367 NspI r_CATG'y Cuts at: 1132 1229 3725 5270 1132 Size: 97 2496 1545 2229 Fragments arranged by size: 2496 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6367 PciI A'CATG_T Cuts at: 5266 5266 Size: 6367 PflMI CCAn_nnn'nTGG Cuts at: 1108 2072 1108 Size: 964 5403 PfoI T'CCnGG_A Cuts at: 2028 2838 2028 Size: 810 5557 PmeI GTTT'AAAC Cuts at: 3671 3671 Size: 6367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4436 4468 5949 5981 4436 Size: 32 1481 32 4822 Fragments arranged by size: 4822 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2808 1613 Size: 1195 5172 PsiI TTA'TAA Cuts at: 3937 3937 Size: 6367 PspOMI G'GGCC_C Cuts at: 2514 3590 2514 Size: 1076 5291 PstI C_TGCA'G Cuts at: 830 2014 2344 2466 3719 830 Size: 1184 330 122 1253 3478 Fragments arranged by size: 3478 1253 1184 330 122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6367 PvuII CAG'CTG Cuts at: 2958 2958 Size: 6367 SacI G_AGCT'C Cuts at: 2738 3302 2738 Size: 564 5803 SalI G'TCGA_C Cuts at: 1936 3709 1936 Size: 1773 4594 SapI GCTCTTCn'nnn_ Cuts at: 2160 2915 3110 2160 Size: 755 195 5417 Fragments arranged by size: 5417 755 195 SbfI CC_TGCA'GG Cuts at: 3719 3719 Size: 6367 ScaI AGT'ACT Cuts at: 4615 6327 4615 Size: 1712 4655 SexAI A'CCwGG_T Cuts at: 2970 2970 Size: 6367 SfcI C'TryA_G Cuts at: 826 1045 2010 2340 2449 2462 3715 4850 5531 Size: 219 965 330 109 13 1253 1135 681 Cuts at: 5531 5722 826 Size: 191 1471 Fragments arranged by size: 1471 1253 1135 965 681 330 219 191 109 13 SfoI GGC'GCC Cuts at: 2869 3253 2869 Size: 384 5983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6367 SgrAI Cr'CCGG_yG Cuts at: 1700 2982 1700 Size: 1282 5085 SmaI CCC'GGG Cuts at: 1872 2408 3694 1872 Size: 536 1286 4545 Fragments arranged by size: 4545 1286 536 SmlI C'TyrA_G Cuts at: 820 1017 1943 2205 2520 4471 5372 5634 820 Size: 197 926 262 315 1951 901 262 1553 Fragments arranged by size: 1951 1553 926 901 315 262 262 197 SnaBI TAC'GTA Cuts at: 493 4158 493 Size: 3665 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6367 SphI G_CATG'C Cuts at: 1132 3725 1132 Size: 2593 3774 SspI AAT'ATT Cuts at: 5 52 4291 5 Size: 47 4239 2081 Fragments arranged by size: 4239 2081 47 StuI AGG'CCT Cuts at: 1517 2506 2543 1517 Size: 989 37 5341 Fragments arranged by size: 5341 989 37 StyI C'CwwG_G Cuts at: 513 1065 2647 3594 3806 513 Size: 552 1582 947 212 3074 Fragments arranged by size: 3074 1582 947 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2051 3245 3257 3329 3341 3509 4394 Size: 1255 471 1194 12 72 12 168 885 Cuts at: 4394 4411 4564 4749 5194 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1194 885 471 445 185 168 153 72 17 12 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4613 6325 96 Size: 274 80 33 51 1443 2636 1712 138 Fragments arranged by size: 2636 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6327 6359 6327 Size: 32 6335 Tth111I GACn'n_nGTC Cuts at: 1316 3224 1316 Size: 1908 4459 XbaI T'CTAG_A Cuts at: 3703 3703 Size: 6367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3613 3613 Size: 6367 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6367 XmaI C'CCGG_G Cuts at: 1870 2406 3692 1870 Size: 536 1286 4545 Fragments arranged by size: 4545 1286 536 XmnI GAAnn'nnTTC Cuts at: 2663 3035 3119 4496 2663 Size: 372 84 1377 4534 Fragments arranged by size: 4534 1377 372 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4175 276 Size: 53 83 186 936 2641 2468 Fragments arranged by size: 2641 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI