Restriction map for
pFN21AB7853
(Circular) (Six-base) MAPSORT of: pFN21AB7853.seq Check: 8968 from: 1 to: 6352
pFN21AB7853 6352 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2867 3239 2867
Size: 372 5980
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3695 1937
Size: 1758 4594
Acc65I G'GTAC_C
Cuts at: 3673 3673
Size: 6352
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 4415 5799 6067 1632
Size: 1302 1481 1384 268 1917
Fragments arranged by size:
1917 1481 1384 1302 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6155
AflIII A'CryG_T
Cuts at: 1203 5251 6228 1203
Size: 4048 977 1327
Fragments arranged by size:
4048 1327 977
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2054 2086 2054
Size: 32 6320
AlwNI CAG_nnn'CTG
Cuts at: 2087 2272 2380 2525 4095 5667 2087
Size: 185 108 145 1570 1572 2772
Fragments arranged by size:
2772 1572 1570 185 145 108
ApaLI G'TGCA_C
Cuts at: 3316 4409 5565 3316
Size: 1093 1156 4103
Fragments arranged by size:
4103 1156 1093
ApoI r'AATT_y
Cuts at: 1493 2509 2798 2899 3262 3661 3887 4039 6148
Size: 1016 289 101 363 399 226 152 2109
Cuts at: 6148 1493
Size: 1697
Fragments arranged by size:
2109 1697 1016 399 363 289 226 152
101
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3601 3677 1870
Size: 73 1658 76 4545
Fragments arranged by size:
4545 1658 76 73
AvrII C'CTAG_G
Cuts at: 2029 2029
Size: 6352
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2485 3673 5128 618
Size: 325 217 627 698 1188 1455 1842
Fragments arranged by size:
1842 1455 1188 698 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2472 2928 3208 3602 3671 1391
Size: 1081 456 280 394 69 4072
Fragments arranged by size:
4072 1081 456 394 280 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3254 928
Size: 971 1355 4026
Fragments arranged by size:
4026 1355 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4543 4577 1903
Size: 34 50 34 2522 34 3678
Fragments arranged by size:
3678 2522 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2408 4244 5454 2408
Size: 1836 1210 3306
Fragments arranged by size:
3306 1836 1210
BclI T'GATC_A
Cuts at: 1548 2622 1548
Size: 1074 5278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3212 4963 136
Size: 107 122 71 631 605 1540 1751 1525
Fragments arranged by size:
1751 1540 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6347 6347
Size: 6352
BlpI GC'TnA_GC
Cuts at: 3769 3769
Size: 6352
Bme1580I G_kGCm'C
Cuts at: 1790 3320 4413 5569 1790
Size: 1530 1093 1156 2573
Fragments arranged by size:
2573 1530 1156 1093
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2062 2722 4083
Size: 416 53 83 399 24 645 660 1361
Cuts at: 4083 5041 442
Size: 958 1753
Fragments arranged by size:
1753 1361 958 660 645 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 3047 2122
Size: 925 5427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2092 2630 2747 3570 3616 5012 1239
Size: 853 538 117 823 46 1396 2579
Fragments arranged by size:
2579 1396 853 823 538 117 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2998 3072 3185 4477 5342 5640 2525
Size: 473 74 113 1292 865 298 3237
Fragments arranged by size:
3237 1292 865 473 298 113 74
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2111 2168 2622 2723 2752 3173 5015
Size: 617 612 57 454 101 29 421 1842
Cuts at: 5015 882
Size: 2219
Fragments arranged by size:
2219 1842 617 612 454 421 101 57
29
BsaAI yAC'GTr
Cuts at: 493 1204 4143 493
Size: 711 2939 2702
Fragments arranged by size:
2939 2702 711
BsaBI GATnn'nnATC
Cuts at: 2396 6346 2396
Size: 3950 2402
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4159 4541 5176 275
Size: 53 83 186 936 2626 382 635 1451
Fragments arranged by size:
2626 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3296 4785 5183 5457 5604 6015 3296
Size: 1489 398 274 147 411 3633
Fragments arranged by size:
3633 1489 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6127
Fragments arranged by size:
6127 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3075 3182 1186
Size: 1889 107 4356
Fragments arranged by size:
4356 1889 107
BseYI C'CCAG_C
Cuts at: 2595 2656 5555 6051 2595
Size: 61 2899 496 2896
Fragments arranged by size:
2899 2896 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5779
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3802 4563 5591 1540
Size: 275 186 1801 761 1028 2301
Fragments arranged by size:
2301 1801 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2928 3320 3602 4413 4498 5569 2928
Size: 392 282 811 85 1071 3711
Fragments arranged by size:
3711 1071 811 392 282 85
BsmI GAATG_Cn'
Cuts at: 3863 3956 3863
Size: 93 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2472 2928 3208 3320 3602 3671 4413
Size: 399 682 456 280 112 282 69 742
Cuts at: 4413 4498 5569 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 682 456 399 282 280
112 85 69
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 2170 4241 5971 2170
Size: 2071 1730 2551
Fragments arranged by size:
2551 2071 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2867 3239 3707 844
Size: 2023 372 468 3489
Fragments arranged by size:
3489 2023 468 372
BsrBI CCG'CTC
Cuts at: 1394 1823 4239 1394
Size: 429 2416 3507
Fragments arranged by size:
3507 2416 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2393 4847 5021 65
Size: 1183 1145 2454 174 1396
Fragments arranged by size:
2454 1396 1183 1145 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4996 5183 1700
Size: 110 3186 187 2869
Fragments arranged by size:
3186 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6352
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 3449 4412 5424 3449
Size: 963 1012 4377
Fragments arranged by size:
4377 1012 963
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3324 794
Size: 2530 3822
BstBI TT'CG_AA
Cuts at: 5156 5156
Size: 6352
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6352
BstYI r'GATC_y
Cuts at: 1972 2241 2250 2292 2716 2844 3623 3682 4049
Size: 269 9 42 424 128 779 59 367
Cuts at: 4049 4434 4451 5903 5989 6001 6347 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 779 424 385 367 346 269
128 86 59 42 17 12 9
Bsu36I CC'TnA_GG
Cuts at: 2490 2518 2667 3036 2490
Size: 28 149 369 5806
Fragments arranged by size:
5806 369 149 28
BtgI C'CryG_G
Cuts at: 513 1065 3210 513
Size: 552 2145 3655
Fragments arranged by size:
3655 2145 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2028 487
Size: 683 56 802 4811
Fragments arranged by size:
4811 802 683 56
BtsI GCAGTG_nn'
Cuts at: 2346 2648 3868 4662 4682 2346
Size: 302 1220 794 20 4016
Fragments arranged by size:
4016 1220 794 302 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6317
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DraIII CAC_nnn'GTG
Cuts at: 2548 3159 2548
Size: 611 5741
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317
Size: 54 1425 50 275 18 1969 889 1629
Cuts at: 6317 8
Size: 43
Fragments arranged by size:
1969 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 2802 3167 4282 1353
Size: 975 474 365 1115 3423
Fragments arranged by size:
3423 1115 975 474 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2926 3600 2926
Size: 674 5678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2092 2630 2747 2934 3570 3616 4415
Size: 393 460 538 117 187 636 46 799
Cuts at: 4415 5012 5799 6067 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 799 787 636 597 538 460 393
268 187 117 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2096 3138 3256 2096
Size: 1042 118 5192
Fragments arranged by size:
5192 1042 118
EcoO109I rG'GnC_Cy
Cuts at: 2026 2368 2522 2635 2026
Size: 342 154 113 5743
Fragments arranged by size:
5743 342 154 113
EcoRI G'AATT_C
Cuts at: 2509 3262 3661 4039 2509
Size: 753 399 378 4822
Fragments arranged by size:
4822 753 399 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 6352
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 5499 1678
Size: 3821 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2403 2408 2425 2435 2440
Size: 32 135 32 831 5 17 10 5
Cuts at: 2440 2457 2498 2530 4993 5025 5067 5099 1373
Size: 17 41 32 2463 32 42 32 2626
Fragments arranged by size:
2626 2463 831 135 42 41 32 32
32 32 32 17 17 10 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2014 3696 3942 669
Size: 988 281 76 1682 246 3079
Fragments arranged by size:
3079 1682 988 281 246 76
HindIII A'AGCT_T
Cuts at: 748 2356 2946 748
Size: 1608 590 4154
Fragments arranged by size:
4154 1608 590
HpaI GTT'AAC
Cuts at: 3942 3942
Size: 6352
KpnI G_GTAC'C
Cuts at: 3677 3677
Size: 6352
MfeI C'AATT_G
Cuts at: 3951 3951
Size: 6352
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 5466 5650 1305
Size: 936 3225 184 2007
Fragments arranged by size:
3225 2007 936 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4530
Fragments arranged by size:
4530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2835 4310 4669 4828 518
Size: 648 1669 1475 359 159 2042
Fragments arranged by size:
2042 1669 1475 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3079 3594 3768 4445 5593 5838 1606
Size: 1473 515 174 677 1148 245 2120
Fragments arranged by size:
2120 1473 1148 677 515 245 174
NcoI C'CATG_G
Cuts at: 513 1065 3210 513
Size: 552 2145 3655
Fragments arranged by size:
3655 2145 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6352
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 2936 3710 5255 1132
Size: 97 1707 774 1545 2229
Fragments arranged by size:
2229 1707 1545 774 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6352
PciI A'CATG_T
Cuts at: 5251 5251
Size: 6352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2327 3159 1108
Size: 1219 832 4301
Fragments arranged by size:
4301 1219 832
PfoI T'CCnGG_A
Cuts at: 2320 2320
Size: 6352
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2368 2635 2368
Size: 267 6085
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 3564 3922 3564
Size: 358 5994
PspXI vC'TCGA_Gb
Cuts at: 3601 3601
Size: 6352
PstI C_TGCA'G
Cuts at: 830 3236 3704 830
Size: 2406 468 3478
Fragments arranged by size:
3478 2406 468
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
PvuII CAG'CTG
Cuts at: 3079 3594 3079
Size: 515 5837
SacI G_AGCT'C
Cuts at: 2928 3602 2928
Size: 674 5678
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SanDI GG'GwC_CC
Cuts at: 2368 2368
Size: 6352
SapI GCTCTTCn'nnn_
Cuts at: 2802 2802
Size: 6352
SbfI CC_TGCA'GG
Cuts at: 3236 3704 3236
Size: 468 5884
ScaI AGT'ACT
Cuts at: 4600 6312 4600
Size: 1712 4640
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 6352
SfcI C'TryA_G
Cuts at: 826 1045 2213 3232 3700 4835 5516 5707 826
Size: 219 1168 1019 468 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 1019 681 468 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 2977 3087 3164 3601 4456
Size: 197 926 561 473 110 77 437 855
Cuts at: 4456 5357 5619 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 855 561 473 437 262
197 110 77
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 2936 3710 1132
Size: 1804 774 3774
Fragments arranged by size:
3774 1804 774
SspI AAT'ATT
Cuts at: 5 52 4276 5
Size: 47 4224 2081
Fragments arranged by size:
4224 2081 47
StuI AGG'CCT
Cuts at: 1517 2034 1517
Size: 517 5835
StyI C'CwwG_G
Cuts at: 513 1065 2029 3210 3791 513
Size: 552 964 1181 581 3074
Fragments arranged by size:
3074 1181 964 581 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2293 4379 4396 4549 4734 5179 325
Size: 1255 713 2086 17 153 185 445 1498
Fragments arranged by size:
2086 1498 1255 713 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4598 6310 96
Size: 274 80 33 51 1443 2621 1712 138
Fragments arranged by size:
2621 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6312 6344 6312
Size: 32 6320
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6352
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2159 2159
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 3601 1943
Size: 1658 4694
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 3503 3587 4481 3503
Size: 84 894 5374
Fragments arranged by size:
5374 894 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PsrI RsrII SacII SfiI SfoI SrfI SwaI