Restriction map for
pFN21AB7852
(Circular) (Six-base) MAPSORT of: pFN21AB7852.seq Check: 6277 from: 1 to: 6547
pFN21AB7852 6547 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3463 3463
Size: 6547
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3890 1937
Size: 1953 4594
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2121 3134 3427 3782 3831 4610 5994
Size: 474 15 1013 293 355 49 779 1384
Cuts at: 5994 6262 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1013 779 474 355 293 268
49 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 5446 6423 1203
Size: 4243 977 1327
Fragments arranged by size:
4243 1327 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 5276 5276
Size: 6547
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6547
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2439 2471 2439
Size: 32 6515
AlwNI CAG_nnn'CTG
Cuts at: 2144 2204 2855 4290 5862 2144
Size: 60 651 1435 1572 2829
Fragments arranged by size:
2829 1572 1435 651 60
ApaI G_GGCC'C
Cuts at: 3755 3755
Size: 6547
ApaLI G'TGCA_C
Cuts at: 4604 5760 4604
Size: 1156 5391
ApoI r'AATT_y
Cuts at: 1493 2480 2576 2806 3286 3856 4082 4234 6343
Size: 987 96 230 480 570 226 152 2109
Cuts at: 6343 1493
Size: 1697
Fragments arranged by size:
2109 1697 987 570 480 230 226 152
96
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 3149 3872 1870
Size: 73 1206 723 4545
Fragments arranged by size:
4545 1206 723 73
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3153 3868 5323 618
Size: 325 217 627 1366 715 1455 1842
Fragments arranged by size:
1842 1455 1366 715 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3755 3866 1391
Size: 2364 111 4072
Fragments arranged by size:
4072 2364 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5576
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4738 4772 1903
Size: 34 2801 34 3678
Fragments arranged by size:
3678 2801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4439 5649 4439
Size: 1210 5337
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6547
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5158 136
Size: 107 122 71 631 605 3486 1525
Fragments arranged by size:
3486 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6542 6542
Size: 6547
BlpI GC'TnA_GC
Cuts at: 3964 3964
Size: 6547
Bme1580I G_kGCm'C
Cuts at: 1790 3755 4608 5764 1790
Size: 1965 853 1156 2573
Fragments arranged by size:
2573 1965 1156 853
BmgBI CAC'GTC
Cuts at: 1318 2131 1318
Size: 813 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2698 3031 3758
Size: 416 53 83 399 24 1281 333 727
Cuts at: 3758 4278 5236 442
Size: 520 958 1753
Fragments arranged by size:
1753 1281 958 727 520 416 399 333
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2898 2898
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 5207 1239
Size: 1379 2589 2579
Fragments arranged by size:
2589 2579 1379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4672 5537 5835 4672
Size: 865 298 5384
Fragments arranged by size:
5384 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5210 882
Size: 617 3711 2219
Fragments arranged by size:
3711 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4338 493
Size: 711 3134 2702
Fragments arranged by size:
3134 2702 711
BsaBI GATnn'nnATC
Cuts at: 3740 6541 3740
Size: 2801 3746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4980 5378 5652 5799 6210 4980
Size: 398 274 147 411 5317
Fragments arranged by size:
5317 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6322
Fragments arranged by size:
6322 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6547
BseYI C'CCAG_C
Cuts at: 3754 5750 6246 3754
Size: 1996 496 4055
Fragments arranged by size:
4055 1996 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2130 2435 1163
Size: 573 394 305 5275
Fragments arranged by size:
5275 573 394 305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3057 3262 3997 4758 5786 1540
Size: 275 186 1056 205 735 761 1028 2301
Fragments arranged by size:
2301 1056 1028 761 735 275 205 186
BsiHKAI G_wGCw'C
Cuts at: 4608 4693 5764 4608
Size: 85 1071 5391
Fragments arranged by size:
5391 1071 85
BsmI GAATG_Cn'
Cuts at: 3793 4058 4151 3793
Size: 265 93 6189
Fragments arranged by size:
6189 265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3755 3866 4608 4693 5764 1391
Size: 399 1965 111 742 85 1071 2174
Fragments arranged by size:
2174 1965 1071 742 399 111 85
BspEI T'CCGG_A
Cuts at: 6210 6210
Size: 6547
BspHI T'CATG_A
Cuts at: 4436 6166 4436
Size: 1730 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3463 3902 844
Size: 2619 439 3489
Fragments arranged by size:
3489 2619 439
BsrBI CCG'CTC
Cuts at: 1394 1823 4434 1394
Size: 429 2611 3507
Fragments arranged by size:
3507 2611 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5042 5216 65
Size: 1183 3794 174 1396
Fragments arranged by size:
3794 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5191 5378 1700
Size: 110 3381 187 2869
Fragments arranged by size:
3381 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3382 3631 96
Size: 3286 249 3012
Fragments arranged by size:
3286 3012 249
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2855 3475 794
Size: 2061 620 3866
Fragments arranged by size:
3866 2061 620
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 2336 1184
Size: 1152 5395
BstXI CCAn_nnnn'nTGG
Cuts at: 2424 2744 2748 3176 3595 2424
Size: 320 4 428 419 5376
Fragments arranged by size:
5376 428 419 320 4
BstYI r'GATC_y
Cuts at: 1972 3117 3877 4244 4629 4646 6098 6184 6196
Size: 1145 760 367 385 17 1452 86 12
Cuts at: 6196 6542 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1145 760 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2460 3826 2460
Size: 1366 5181
BtgI C'CryG_G
Cuts at: 513 1065 2604 3175 3501 513
Size: 552 1539 571 326 3559
Fragments arranged by size:
3559 1539 571 552 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2730 487
Size: 683 56 1504 4304
Fragments arranged by size:
4304 1504 683 56
BtsI GCAGTG_nn'
Cuts at: 3550 4063 4857 4877 3550
Size: 513 794 20 5220
Fragments arranged by size:
5220 794 513 20
ClaI AT'CG_AT
Cuts at: 1554 3687 1554
Size: 2133 4414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6512
DraI TTT'AAA
Cuts at: 2869 3851 2869
Size: 982 5565
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3994 4883 6512
Size: 54 1425 50 275 18 2164 889 1629
Cuts at: 6512 8
Size: 43
Fragments arranged by size:
2164 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 3307 3446 3645 3648 4477 1353
Size: 729 1225 139 199 3 829 3423
Fragments arranged by size:
3423 1225 829 729 199 139 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5134 5508 5654 387
Size: 1064 138 3545 374 146 1280
Fragments arranged by size:
3545 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2121 2618 3134 3427 3782 3831
Size: 393 474 15 497 516 293 355 49
Cuts at: 3831 4610 5207 5994 6262 1239
Size: 779 597 787 268 1524
Fragments arranged by size:
1524 787 779 597 516 497 474 393
355 293 268 49 15
EcoO109I rG'GnC_Cy
Cuts at: 3526 3526
Size: 6547
EcoRI G'AATT_C
Cuts at: 3856 4234 3856
Size: 378 6169
EcoRV GAT'ATC
Cuts at: 3046 3046
Size: 6547
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 5694 1678
Size: 4016 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3809 3841 5188 5220 5262
Size: 32 135 32 2237 32 1347 32 42
Cuts at: 5262 5294 1373
Size: 32 2626
Fragments arranged by size:
2626 2237 1347 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2030 748
Size: 1282 5265
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2566 3584 5661 5845 1305
Size: 1261 1018 2077 184 2007
Fragments arranged by size:
2077 2007 1261 1018 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4725
Fragments arranged by size:
4725 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 2358 2994 3012 3174 3201 4505
Size: 648 1081 111 636 18 162 27 1304
Cuts at: 4505 4864 5023 518
Size: 359 159 2042
Fragments arranged by size:
2042 1304 1081 648 636 359 162 159
111 27 18
MspA1I CmG'CkG
Cuts at: 1606 2141 3963 4640 5788 6033 1606
Size: 535 1822 677 1148 245 2120
Fragments arranged by size:
2120 1822 1148 677 535 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5995
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 3662 3905 5450 1132
Size: 97 2433 243 1545 2229
Fragments arranged by size:
2433 2229 1545 243 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6547
PciI A'CATG_T
Cuts at: 5446 5446
Size: 6547
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6547
PfoI T'CCnGG_A
Cuts at: 2813 2813
Size: 6547
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2970 3002 4616 4648 6129 6161 2970
Size: 32 1614 32 1481 32 3356
Fragments arranged by size:
3356 1614 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3526 3526
Size: 6547
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6547
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspOMI G'GGCC_C
Cuts at: 3751 3751
Size: 6547
PstI C_TGCA'G
Cuts at: 830 3899 830
Size: 3069 3478
PvuI CG_AT'CG
Cuts at: 2001 3057 3262 2001
Size: 1056 205 5286
Fragments arranged by size:
5286 1056 205
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SapI GCTCTTCn'nnn_
Cuts at: 3648 3648
Size: 6547
SbfI CC_TGCA'GG
Cuts at: 3899 3899
Size: 6547
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SfcI C'TryA_G
Cuts at: 826 1045 2319 3328 3895 5030 5711 5902 826
Size: 219 1274 1009 567 1135 681 191 1471
Fragments arranged by size:
1471 1274 1135 1009 681 567 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3151 3874 1872
Size: 1279 723 4545
Fragments arranged by size:
4545 1279 723
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4651 5552 5814 820
Size: 197 926 2708 901 262 1553
Fragments arranged by size:
2708 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 2671 152
Size: 2519 4028
SphI G_CATG'C
Cuts at: 1132 3662 3905 1132
Size: 2530 243 3774
Fragments arranged by size:
3774 2530 243
SspI AAT'ATT
Cuts at: 5 52 4471 5
Size: 47 4419 2081
Fragments arranged by size:
4419 2081 47
StuI AGG'CCT
Cuts at: 1517 2507 1517
Size: 990 5557
StyI C'CwwG_G
Cuts at: 513 1065 2103 2220 2625 2785 3986 513
Size: 552 1038 117 405 160 1201 3074
Fragments arranged by size:
3074 1201 1038 552 405 160 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3013 4574 4591 4744 4929 5374 325
Size: 1255 1433 1561 17 153 185 445 1498
Fragments arranged by size:
1561 1498 1433 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3382 3631 4793
Size: 274 80 33 51 1443 1405 249 1162
Cuts at: 4793 6505 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1405 1162 274 249 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3064 3096 6507 6539 3064
Size: 32 3411 32 3072
Fragments arranged by size:
3411 3072 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3725 1316
Size: 2409 4138
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 2053
Size: 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 3149 3872 1870
Size: 1279 723 4545
Fragments arranged by size:
4545 1279 723
XmnI GAAnn'nnTTC
Cuts at: 2537 4676 2537
Size: 2139 4408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI