(Circular) (Six-base) MAPSORT of: pFN21AB7852.seq Check: 6277 from: 1 to: 6547 pFN21AB7852 6547 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3463 3463 Size: 6547 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3890 1937 Size: 1953 4594 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2121 3134 3427 3782 3831 4610 5994 Size: 474 15 1013 293 355 49 779 1384 Cuts at: 5994 6262 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1013 779 474 355 293 268 49 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 5446 6423 1203 Size: 4243 977 1327 Fragments arranged by size: 4243 1327 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6547 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2439 2471 2439 Size: 32 6515 AlwNI CAG_nnn'CTG Cuts at: 2144 2204 2855 4290 5862 2144 Size: 60 651 1435 1572 2829 Fragments arranged by size: 2829 1572 1435 651 60 ApaI G_GGCC'C Cuts at: 3755 3755 Size: 6547 ApaLI G'TGCA_C Cuts at: 4604 5760 4604 Size: 1156 5391 ApoI r'AATT_y Cuts at: 1493 2480 2576 2806 3286 3856 4082 4234 6343 Size: 987 96 230 480 570 226 152 2109 Cuts at: 6343 1493 Size: 1697 Fragments arranged by size: 2109 1697 987 570 480 230 226 152 96 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 3149 3872 1870 Size: 73 1206 723 4545 Fragments arranged by size: 4545 1206 723 73 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3153 3868 5323 618 Size: 325 217 627 1366 715 1455 1842 Fragments arranged by size: 1842 1455 1366 715 627 325 217 BanII G_rGCy'C Cuts at: 1391 3755 3866 1391 Size: 2364 111 4072 Fragments arranged by size: 4072 2364 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5576 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4738 4772 1903 Size: 34 2801 34 3678 Fragments arranged by size: 3678 2801 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 1548 Size: 6547 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5158 136 Size: 107 122 71 631 605 3486 1525 Fragments arranged by size: 3486 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 3964 3964 Size: 6547 Bme1580I G_kGCm'C Cuts at: 1790 3755 4608 5764 1790 Size: 1965 853 1156 2573 Fragments arranged by size: 2573 1965 1156 853 BmgBI CAC'GTC Cuts at: 1318 2131 1318 Size: 813 5734 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2698 3031 3758 Size: 416 53 83 399 24 1281 333 727 Cuts at: 3758 4278 5236 442 Size: 520 958 1753 Fragments arranged by size: 1753 1281 958 727 520 416 399 333 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2898 2898 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 5207 1239 Size: 1379 2589 2579 Fragments arranged by size: 2589 2579 1379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4672 5537 5835 4672 Size: 865 298 5384 Fragments arranged by size: 5384 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5210 882 Size: 617 3711 2219 Fragments arranged by size: 3711 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4338 493 Size: 711 3134 2702 Fragments arranged by size: 3134 2702 711 BsaBI GATnn'nnATC Cuts at: 3740 6541 3740 Size: 2801 3746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4980 5378 5652 5799 6210 4980 Size: 398 274 147 411 5317 Fragments arranged by size: 5317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6322 Fragments arranged by size: 6322 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6547 BseYI C'CCAG_C Cuts at: 3754 5750 6246 3754 Size: 1996 496 4055 Fragments arranged by size: 4055 1996 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2130 2435 1163 Size: 573 394 305 5275 Fragments arranged by size: 5275 573 394 305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3057 3262 3997 4758 5786 1540 Size: 275 186 1056 205 735 761 1028 2301 Fragments arranged by size: 2301 1056 1028 761 735 275 205 186 BsiHKAI G_wGCw'C Cuts at: 4608 4693 5764 4608 Size: 85 1071 5391 Fragments arranged by size: 5391 1071 85 BsmI GAATG_Cn' Cuts at: 3793 4058 4151 3793 Size: 265 93 6189 Fragments arranged by size: 6189 265 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3755 3866 4608 4693 5764 1391 Size: 399 1965 111 742 85 1071 2174 Fragments arranged by size: 2174 1965 1071 742 399 111 85 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 4436 6166 4436 Size: 1730 4817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3463 3902 844 Size: 2619 439 3489 Fragments arranged by size: 3489 2619 439 BsrBI CCG'CTC Cuts at: 1394 1823 4434 1394 Size: 429 2611 3507 Fragments arranged by size: 3507 2611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5042 5216 65 Size: 1183 3794 174 1396 Fragments arranged by size: 3794 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5191 5378 1700 Size: 110 3381 187 2869 Fragments arranged by size: 3381 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3382 3631 96 Size: 3286 249 3012 Fragments arranged by size: 3286 3012 249 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 2855 3475 794 Size: 2061 620 3866 Fragments arranged by size: 3866 2061 620 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 2336 1184 Size: 1152 5395 BstXI CCAn_nnnn'nTGG Cuts at: 2424 2744 2748 3176 3595 2424 Size: 320 4 428 419 5376 Fragments arranged by size: 5376 428 419 320 4 BstYI r'GATC_y Cuts at: 1972 3117 3877 4244 4629 4646 6098 6184 6196 Size: 1145 760 367 385 17 1452 86 12 Cuts at: 6196 6542 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1145 760 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2460 3826 2460 Size: 1366 5181 BtgI C'CryG_G Cuts at: 513 1065 2604 3175 3501 513 Size: 552 1539 571 326 3559 Fragments arranged by size: 3559 1539 571 552 326 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2730 487 Size: 683 56 1504 4304 Fragments arranged by size: 4304 1504 683 56 BtsI GCAGTG_nn' Cuts at: 3550 4063 4857 4877 3550 Size: 513 794 20 5220 Fragments arranged by size: 5220 794 513 20 ClaI AT'CG_AT Cuts at: 1554 3687 1554 Size: 2133 4414 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 2869 3851 2869 Size: 982 5565 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3994 4883 6512 Size: 54 1425 50 275 18 2164 889 1629 Cuts at: 6512 8 Size: 43 Fragments arranged by size: 2164 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 3307 3446 3645 3648 4477 1353 Size: 729 1225 139 199 3 829 3423 Fragments arranged by size: 3423 1225 829 729 199 139 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5134 5508 5654 387 Size: 1064 138 3545 374 146 1280 Fragments arranged by size: 3545 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2121 2618 3134 3427 3782 3831 Size: 393 474 15 497 516 293 355 49 Cuts at: 3831 4610 5207 5994 6262 1239 Size: 779 597 787 268 1524 Fragments arranged by size: 1524 787 779 597 516 497 474 393 355 293 268 49 15 EcoO109I rG'GnC_Cy Cuts at: 3526 3526 Size: 6547 EcoRI G'AATT_C Cuts at: 3856 4234 3856 Size: 378 6169 EcoRV GAT'ATC Cuts at: 3046 3046 Size: 6547 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 5694 1678 Size: 4016 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3809 3841 5188 5220 5262 Size: 32 135 32 2237 32 1347 32 42 Cuts at: 5262 5294 1373 Size: 32 2626 Fragments arranged by size: 2626 2237 1347 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 2030 748 Size: 1282 5265 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2566 3584 5661 5845 1305 Size: 1261 1018 2077 184 2007 Fragments arranged by size: 2077 2007 1261 1018 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4725 Fragments arranged by size: 4725 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 2358 2994 3012 3174 3201 4505 Size: 648 1081 111 636 18 162 27 1304 Cuts at: 4505 4864 5023 518 Size: 359 159 2042 Fragments arranged by size: 2042 1304 1081 648 636 359 162 159 111 27 18 MspA1I CmG'CkG Cuts at: 1606 2141 3963 4640 5788 6033 1606 Size: 535 1822 677 1148 245 2120 Fragments arranged by size: 2120 1822 1148 677 535 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5995 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 3662 3905 5450 1132 Size: 97 2433 243 1545 2229 Fragments arranged by size: 2433 2229 1545 243 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6547 PciI A'CATG_T Cuts at: 5446 5446 Size: 6547 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6547 PfoI T'CCnGG_A Cuts at: 2813 2813 Size: 6547 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2970 3002 4616 4648 6129 6161 2970 Size: 32 1614 32 1481 32 3356 Fragments arranged by size: 3356 1614 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3526 3526 Size: 6547 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspOMI G'GGCC_C Cuts at: 3751 3751 Size: 6547 PstI C_TGCA'G Cuts at: 830 3899 830 Size: 3069 3478 PvuI CG_AT'CG Cuts at: 2001 3057 3262 2001 Size: 1056 205 5286 Fragments arranged by size: 5286 1056 205 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SapI GCTCTTCn'nnn_ Cuts at: 3648 3648 Size: 6547 SbfI CC_TGCA'GG Cuts at: 3899 3899 Size: 6547 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SfcI C'TryA_G Cuts at: 826 1045 2319 3328 3895 5030 5711 5902 826 Size: 219 1274 1009 567 1135 681 191 1471 Fragments arranged by size: 1471 1274 1135 1009 681 567 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3151 3874 1872 Size: 1279 723 4545 Fragments arranged by size: 4545 1279 723 SmlI C'TyrA_G Cuts at: 820 1017 1943 4651 5552 5814 820 Size: 197 926 2708 901 262 1553 Fragments arranged by size: 2708 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 2671 152 Size: 2519 4028 SphI G_CATG'C Cuts at: 1132 3662 3905 1132 Size: 2530 243 3774 Fragments arranged by size: 3774 2530 243 SspI AAT'ATT Cuts at: 5 52 4471 5 Size: 47 4419 2081 Fragments arranged by size: 4419 2081 47 StuI AGG'CCT Cuts at: 1517 2507 1517 Size: 990 5557 StyI C'CwwG_G Cuts at: 513 1065 2103 2220 2625 2785 3986 513 Size: 552 1038 117 405 160 1201 3074 Fragments arranged by size: 3074 1201 1038 552 405 160 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3013 4574 4591 4744 4929 5374 325 Size: 1255 1433 1561 17 153 185 445 1498 Fragments arranged by size: 1561 1498 1433 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3382 3631 4793 Size: 274 80 33 51 1443 1405 249 1162 Cuts at: 4793 6505 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1405 1162 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3064 3096 6507 6539 3064 Size: 32 3411 32 3072 Fragments arranged by size: 3411 3072 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3725 1316 Size: 2409 4138 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2053 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 3149 3872 1870 Size: 1279 723 4545 Fragments arranged by size: 4545 1279 723 XmnI GAAnn'nnTTC Cuts at: 2537 4676 2537 Size: 2139 4408 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI