Restriction map for
pFN21AB7850
(Circular) (Six-base) MAPSORT of: pFN21AB7850.seq Check: 9192 from: 1 to: 6574
pFN21AB7850 6574 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 3917 1937
Size: 187 1793 4594
Fragments arranged by size:
4594 1793 187
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2018 2034 2744 3440 3621 3633 3843 4637
Size: 386 16 710 696 181 12 210 794
Cuts at: 4637 6021 6289 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 794 710 696 386 268 210
181 16 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 2770 3380 3382 3619 5473 6450 1203
Size: 1567 610 2 237 1854 977 1327
Fragments arranged by size:
1854 1567 1327 977 610 237 2
AgeI A'CCGG_T
Cuts at: 2097 5405 2097
Size: 3308 3266
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 3543 1166
Size: 2377 4197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AlwNI CAG_nnn'CTG
Cuts at: 2498 3755 4317 5889 2498
Size: 1257 562 1572 3183
Fragments arranged by size:
3183 1572 1257 562
ApaLI G'TGCA_C
Cuts at: 3527 4631 5787 3527
Size: 1104 1156 4314
Fragments arranged by size:
4314 1156 1104
ApoI r'AATT_y
Cuts at: 1493 2186 2982 3591 3883 4109 4261 6370 1493
Size: 693 796 609 292 226 152 2109 1697
Fragments arranged by size:
2109 1697 796 693 609 292 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2285 5128 160
Size: 872 1253 2843 1606
Fragments arranged by size:
2843 1606 1253 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 3899 1870
Size: 73 1956 4545
Fragments arranged by size:
4545 1956 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3600 3633 3600
Size: 33 6541
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2528 3895 5350 618
Size: 325 217 627 741 1367 1455 1842
Fragments arranged by size:
1842 1455 1367 741 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3893 1391
Size: 2502 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5603
BbvCI CC'TCA_GC
Cuts at: 3759 3789 3759
Size: 30 6544
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4765 4799 1903
Size: 34 2828 34 3678
Fragments arranged by size:
3678 2828 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5185 136
Size: 107 122 71 631 605 337 3176 1525
Fragments arranged by size:
3176 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3505 6569 3505
Size: 3064 3510
BlpI GC'TnA_GC
Cuts at: 3991 3991
Size: 6574
Bme1580I G_kGCm'C
Cuts at: 1790 3531 4635 5791 1790
Size: 1741 1104 1156 2573
Fragments arranged by size:
2573 1741 1156 1104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2982 4305 5263
Size: 416 53 83 399 24 1565 1323 958
Cuts at: 5263 442
Size: 1753
Fragments arranged by size:
1753 1565 1323 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3165 3270 3497 5234 1239
Size: 1926 105 227 1737 2579
Fragments arranged by size:
2579 1926 1737 227 105
Bpu10I CC'TnA_GC
Cuts at: 3759 3789 3759
Size: 30 6544
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4699 5564 5862 4699
Size: 865 298 5411
Fragments arranged by size:
5411 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3794 5237 882
Size: 617 2295 1443 2219
Fragments arranged by size:
2295 2219 1443 617
BsaAI yAC'GTr
Cuts at: 493 1204 3383 3703 4365 493
Size: 711 2179 320 662 2702
Fragments arranged by size:
2702 2179 711 662 320
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4381 4763 5398 275
Size: 53 83 186 936 2848 382 635 1451
Fragments arranged by size:
2848 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2097 5007 5405 5679 5826 6237 2097
Size: 2910 398 274 147 411 2434
Fragments arranged by size:
2910 2434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3722 3752 1178
Size: 30 165 30 2319 30 4000
Fragments arranged by size:
4000 2319 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3641 3743 1186
Size: 2455 102 4017
Fragments arranged by size:
4017 2455 102
BseYI C'CCAG_C
Cuts at: 3213 5777 6273 3213
Size: 2564 496 3514
Fragments arranged by size:
3514 2564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6001
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2071 4024 4785 5813 1540
Size: 275 186 70 1953 761 1028 2301
Fragments arranged by size:
2301 1953 1028 761 275 186 70
BsiHKAI G_wGCw'C
Cuts at: 2612 2841 3531 4635 4720 5791 2612
Size: 229 690 1104 85 1071 3395
Fragments arranged by size:
3395 1104 1071 690 229 85
BsmI GAATG_Cn'
Cuts at: 2033 3188 4085 4178 2033
Size: 1155 897 93 4429
Fragments arranged by size:
4429 1155 897 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2012 2012
Size: 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2612 2841 3531 3893 4635 4720 5791
Size: 399 822 229 690 362 742 85 1071
Cuts at: 5791 1391
Size: 2174
Fragments arranged by size:
2174 1071 822 742 690 399 362 229
85
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 3088 3717 4463 6193 3088
Size: 629 746 1730 3469
Fragments arranged by size:
3469 1730 746 629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3550 3929 844
Size: 2706 379 3489
Fragments arranged by size:
3489 2706 379
BsrBI CCG'CTC
Cuts at: 1394 1823 4461 1394
Size: 429 2638 3507
Fragments arranged by size:
3507 2638 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2474 3449 5069 5243 65
Size: 1183 1226 975 1620 174 1396
Fragments arranged by size:
1620 1396 1226 1183 975 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2097 5218 5405 1700
Size: 110 287 3121 187 2869
Fragments arranged by size:
3121 2869 287 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6574
BssSI C'ACGA_G
Cuts at: 2722 4634 5646 2722
Size: 1912 1012 3650
Fragments arranged by size:
3650 1912 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3755 794
Size: 2961 3613
BstBI TT'CG_AA
Cuts at: 3046 5378 3046
Size: 2332 4242
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstXI CCAn_nnnn'nTGG
Cuts at: 2539 2539
Size: 6574
BstYI r'GATC_y
Cuts at: 1972 3505 3904 4271 4656 4673 6125 6211 6223
Size: 1533 399 367 385 17 1452 86 12
Cuts at: 6223 6569 1972
Size: 346 1977
Fragments arranged by size:
1977 1533 1452 399 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5083
Fragments arranged by size:
5083 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 3129 4090 4884 4904 3129
Size: 961 794 20 4799
Fragments arranged by size:
4799 961 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 2815 3034 3878 2815
Size: 219 844 5511
Fragments arranged by size:
5511 844 219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4021 4910 6539
Size: 54 1425 50 275 18 2191 889 1629
Cuts at: 6539 8
Size: 43
Fragments arranged by size:
2191 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 4504 1353
Size: 657 2494 3423
Fragments arranged by size:
3423 2494 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5161 5535 5681 387
Size: 1064 138 3572 374 146 1280
Fragments arranged by size:
3572 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2018 2034 2744 3165 3270 3440 3497
Size: 393 386 16 710 421 105 170 57
Cuts at: 3497 3621 3633 3843 4637 5234 6021 6289 1239
Size: 124 12 210 794 597 787 268 1524
Fragments arranged by size:
1524 794 787 710 597 421 393 386
268 210 170 124 105 57 16 12
EcoRI G'AATT_C
Cuts at: 3591 3883 4261 3591
Size: 292 378 5904
Fragments arranged by size:
5904 378 292
EcoRV GAT'ATC
Cuts at: 2894 2894
Size: 6574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3059 3383 3059
Size: 324 6250
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 5721 1678
Size: 4043 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3722 3754 5215 5247 5289
Size: 32 135 32 2150 32 1461 32 42
Cuts at: 5289 5321 1373
Size: 32 2626
Fragments arranged by size:
2626 2150 1461 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2125 3918 4164 669
Size: 988 281 187 1793 246 3079
Fragments arranged by size:
3079 1793 988 281 246 187
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5688 5872 1305
Size: 4383 184 2007
Fragments arranged by size:
4383 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4752
Fragments arranged by size:
4752 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 3057 3321 3543 3783 4532 4891
Size: 648 1371 520 264 222 240 749 359
Cuts at: 4891 5050 518
Size: 159 2042
Fragments arranged by size:
2042 1371 749 648 520 359 264 240
222 159
MspA1I CmG'CkG
Cuts at: 1606 2764 3213 3990 4667 5815 6060 1606
Size: 1158 449 777 677 1148 245 2120
Fragments arranged by size:
2120 1158 1148 777 677 449 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5083
Fragments arranged by size:
5083 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2774 3138 3443 3623 3638 3932 5477
Size: 97 1545 364 305 180 15 294 1545
Cuts at: 5477 1132
Size: 2229
Fragments arranged by size:
2229 1545 1545 364 305 294 180 97
15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6574
PciI A'CATG_T
Cuts at: 2770 3619 5473 2770
Size: 849 1854 3871
Fragments arranged by size:
3871 1854 849
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6574
PfoI T'CCnGG_A
Cuts at: 3513 3513
Size: 6574
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PmlI CAC'GTG
Cuts at: 3383 3703 3383
Size: 320 6254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2631 4643 4675 6156 6188 2599
Size: 32 2012 32 1481 32 2985
Fragments arranged by size:
2985 2012 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PstI C_TGCA'G
Cuts at: 830 2488 3926 830
Size: 1658 1438 3478
Fragments arranged by size:
3478 1658 1438
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2764 3213 2764
Size: 449 6125
SalI G'TCGA_C
Cuts at: 1936 2123 3916 1936
Size: 187 1793 4594
Fragments arranged by size:
4594 1793 187
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 2999 4822 6534 2999
Size: 1823 1712 3039
Fragments arranged by size:
3039 1823 1712
SfcI C'TryA_G
Cuts at: 826 1045 2484 3922 5057 5738 5929 826
Size: 219 1439 1438 1135 681 191 1471
Fragments arranged by size:
1471 1439 1438 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 3901 1872
Size: 2029 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4678 5579 5841 820
Size: 197 926 2735 901 262 1553
Fragments arranged by size:
2735 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3138 3932 1132
Size: 2006 794 3774
Fragments arranged by size:
3774 2006 794
SspI AAT'ATT
Cuts at: 5 52 3337 4498 5
Size: 47 3285 1161 2081
Fragments arranged by size:
3285 2081 1161 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 2004 4013 513
Size: 552 939 2009 3074
Fragments arranged by size:
3074 2009 939 552
SwaI ATTT'AAAT
Cuts at: 2815 2815
Size: 6574
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 4601 4618 4771 4956 5401 325
Size: 1255 505 2516 17 153 185 445 1498
Fragments arranged by size:
2516 1498 1255 505 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2598 2997 3269
Size: 274 80 33 51 1443 621 399 272
Cuts at: 3269 3616 3667 4820 6532 96
Size: 347 51 1153 1712 138
Fragments arranged by size:
1712 1443 1153 621 399 347 274 272
138 80 51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6534 6566 6534
Size: 32 6542
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3577 3682 3910 3577
Size: 105 228 6241
Fragments arranged by size:
6241 228 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3811 3811
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 3899 1870
Size: 2029 4545
XmnI GAAnn'nnTTC
Cuts at: 2031 3042 3363 4703 2031
Size: 1011 321 1340 3902
Fragments arranged by size:
3902 1340 1011 321
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI