Restriction map for
pFN21AB7828
(Circular) (Six-base) MAPSORT of: pFN21AB7828.seq Check: 5827 from: 1 to: 6520
pFN21AB7828 6520 bp
With 182 enzymes: SgfI *
April 10, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2917 2917
Size: 6520
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3863 1937
Size: 1926 4594
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 3819 4647 5020 1563
Size: 2256 828 373 3063
Fragments arranged by size:
3063 2256 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2104 2749 3153 4583 5967 6235 1632
Size: 472 645 404 1430 1384 268 1917
Fragments arranged by size:
1917 1430 1384 645 472 404 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 5419 6396 1203
Size: 4216 977 1327
Fragments arranged by size:
4216 1327 977
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 3251 1166
Size: 2085 4435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2915 3269 1184
Size: 1731 354 4435
Fragments arranged by size:
4435 1731 354
AlwNI CAG_nnn'CTG
Cuts at: 4263 5835 4263
Size: 1572 4948
ApaI G_GGCC'C
Cuts at: 2952 2952
Size: 6520
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2249 2818 2855 3017 3829 4055 4207 6316
Size: 756 569 37 162 812 226 152 2109
Cuts at: 6316 1493
Size: 1697
Fragments arranged by size:
2109 1697 812 756 569 226 162 152
37
AseI AT'TA_AT
Cuts at: 160 1032 3494 5074 160
Size: 872 2462 1580 1606
Fragments arranged by size:
2462 1606 1580 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 3845 1870
Size: 73 1902 4545
Fragments arranged by size:
4545 1902 73
BamHI G'GATC_C
Cuts at: 3850 4217 3850
Size: 367 6153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3841 5296 618
Size: 325 217 627 2054 1455 1842
Fragments arranged by size:
2054 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2465 2883 2952 3839 1391
Size: 1074 418 69 887 4072
Fragments arranged by size:
4072 1074 887 418 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2461 2793 928
Size: 971 562 332 4655
Fragments arranged by size:
4655 971 562 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5131 136
Size: 107 122 71 631 605 3459 1525
Fragments arranged by size:
3459 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2089 6515 2089
Size: 4426 2094
BlpI GC'TnA_GC
Cuts at: 2877 3937 2877
Size: 1060 5460
Bme1580I G_kGCm'C
Cuts at: 1790 2952 3157 4581 5737 1790
Size: 1162 205 1424 1156 2573
Fragments arranged by size:
2573 1424 1162 1156 205
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2459 2877 3054
Size: 416 53 83 399 24 1042 418 177
Cuts at: 3054 4251 5209 442
Size: 1197 958 1753
Fragments arranged by size:
1753 1197 1042 958 418 416 399 177
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5180 1239
Size: 3941 2579
Bpu10I CC'TnA_GC
Cuts at: 2514 2514
Size: 6520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4645 5510 5808 4645
Size: 865 298 5357
Fragments arranged by size:
5357 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5183 882
Size: 617 3684 2219
Fragments arranged by size:
3684 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3253 3509 3710 4311 493
Size: 711 2049 256 201 601 2702
Fragments arranged by size:
2702 2049 711 601 256 201
BsaBI GATnn'nnATC
Cuts at: 2088 3591 6514 2088
Size: 1503 2923 2094
Fragments arranged by size:
2923 2094 1503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4327 4709 5344 275
Size: 53 83 186 936 2794 382 635 1451
Fragments arranged by size:
2794 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4953 5351 5625 5772 6183 4953
Size: 398 274 147 411 5290
Fragments arranged by size:
5290 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6295
Fragments arranged by size:
6295 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2585 1186
Size: 1399 5121
BseYI C'CCAG_C
Cuts at: 2043 2271 5723 6219 2043
Size: 228 3452 496 2344
Fragments arranged by size:
3452 2344 496 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5947
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3970 4731 5759 1540
Size: 275 186 1969 761 1028 2301
Fragments arranged by size:
2301 1969 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3248 4581 4666 5737 3248
Size: 1333 85 1071 4031
Fragments arranged by size:
4031 1333 1071 85
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2465 2883 2952 3157 3248 3839 4581
Size: 399 675 418 69 205 91 591 742
Cuts at: 4581 4666 5737 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 675 591 418 399 205
91 85 69
BspEI T'CCGG_A
Cuts at: 6183 6183
Size: 6520
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2917 3875 844
Size: 2073 958 3489
Fragments arranged by size:
3489 2073 958
BsrBI CCG'CTC
Cuts at: 1394 1823 4407 1394
Size: 429 2584 3507
Fragments arranged by size:
3507 2584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2472 3778 5015 5189 65
Size: 1183 1224 1306 1237 174 1396
Fragments arranged by size:
1396 1306 1237 1224 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5164 5351 1700
Size: 110 3354 187 2869
Fragments arranged by size:
3354 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6520
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6520
BstYI r'GATC_y
Cuts at: 1972 2089 2297 3850 4217 4602 4619 6071 6157
Size: 117 208 1553 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1553 1452 385 367 346 208 117
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2637 513
Size: 552 1572 4396
Fragments arranged by size:
4396 1572 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5781
Fragments arranged by size:
5781 683 56
BtsI GCAGTG_nn'
Cuts at: 4036 4830 4850 4036
Size: 794 20 5706
Fragments arranged by size:
5706 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DraIII CAC_nnn'GTG
Cuts at: 3085 3085
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5527 809
Size: 507 4211 1802
Fragments arranged by size:
4211 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3967 4856 6485
Size: 54 1425 50 275 18 2137 889 1629
Cuts at: 6485 8
Size: 43
Fragments arranged by size:
2137 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 3135 4450 1353
Size: 879 903 1315 3423
Fragments arranged by size:
3423 1315 903 879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5107 5481 5627 387
Size: 1064 138 3518 374 146 1280
Fragments arranged by size:
3518 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2104 2749 3153 4583 5180 5967 6235
Size: 393 472 645 404 1430 597 787 268
Cuts at: 6235 1239
Size: 1524
Fragments arranged by size:
1524 1430 787 645 597 472 404 393
268
EcoRI G'AATT_C
Cuts at: 3017 3829 4207 3017
Size: 812 378 5330
Fragments arranged by size:
5330 812 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3503 3503
Size: 6520
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 5667 1678
Size: 3989 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 3017 3049 5161
Size: 32 135 32 992 32 421 32 2112
Cuts at: 5161 5193 5235 5267 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2112 992 421 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 2802 2836 3442 3864 4110
Size: 988 281 736 128 34 606 422 246
Cuts at: 4110 669
Size: 3079
Fragments arranged by size:
3079 988 736 606 422 281 246 128
34
HindIII A'AGCT_T
Cuts at: 748 2982 748
Size: 2234 4286
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5634 5818 1305
Size: 4329 184 2007
Fragments arranged by size:
4329 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4698
Fragments arranged by size:
4698 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 3255 4478 4837 4996 518
Size: 648 2085 4 1223 359 159 2042
Fragments arranged by size:
2085 2042 1223 648 359 159 4
MspA1I CmG'CkG
Cuts at: 1606 3936 4613 5761 6006 1606
Size: 2330 677 1148 245 2120
Fragments arranged by size:
2330 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2637 513
Size: 552 1572 4396
Fragments arranged by size:
4396 1572 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 2365 2902 3706 3878 5423 1132
Size: 97 1136 537 804 172 1545 2229
Fragments arranged by size:
2229 1545 1136 804 537 172 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6520
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6520
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PmlI CAC'GTG
Cuts at: 3253 3253
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4589 4621 6102 6134 4589
Size: 32 1481 32 4975
Fragments arranged by size:
4975 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2038 2121 1613
Size: 425 83 6012
Fragments arranged by size:
6012 425 83
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PspOMI G'GGCC_C
Cuts at: 2948 2948
Size: 6520
PstI C_TGCA'G
Cuts at: 830 3756 3872 830
Size: 2926 116 3478
Fragments arranged by size:
3478 2926 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SapI GCTCTTCn'nnn_
Cuts at: 3135 3135
Size: 6520
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SfcI C'TryA_G
Cuts at: 826 1045 2728 3411 3752 3868 5003 5684 5875
Size: 219 1683 683 341 116 1135 681 191
Cuts at: 5875 826
Size: 1471
Fragments arranged by size:
1683 1471 1135 683 681 341 219 191
116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 3847 1872
Size: 1975 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4624 5525 5787 820
Size: 197 926 2681 901 262 1553
Fragments arranged by size:
2681 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3509 4311 493
Size: 3016 802 2702
Fragments arranged by size:
3016 2702 802
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 2365 2902 3706 3878 1132
Size: 1233 537 804 172 3774
Fragments arranged by size:
3774 1233 804 537 172
SspI AAT'ATT
Cuts at: 5 52 2608 4444 5
Size: 47 2556 1836 2081
Fragments arranged by size:
2556 2081 1836 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6520
StyI C'CwwG_G
Cuts at: 513 1065 2637 3959 513
Size: 552 1572 1322 3074
Fragments arranged by size:
3074 1572 1322 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4547 4564 4717 4902 5347 325
Size: 1255 2967 17 153 185 445 1498
Fragments arranged by size:
2967 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 3193 4766
Size: 274 80 33 51 1443 369 847 1573
Cuts at: 4766 6478 96
Size: 1712 138
Fragments arranged by size:
1712 1573 1443 847 369 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6480 6512 6480
Size: 32 6488
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3856 3856
Size: 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6520
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 3845 1870
Size: 1975 4545
XmnI GAAnn'nnTTC
Cuts at: 4649 4649
Size: 6520
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI