Restriction map for
pFN21AB7826
(Circular) (Six-base) MAPSORT of: pFN21AB7826.seq Check: 9489 from: 1 to: 6517
pFN21AB7826 6517 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4327 278
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3860 1937
Size: 1923 4594
Acc65I G'GTAC_C
Cuts at: 3838 3838
Size: 6517
AclI AA'CG_TT
Cuts at: 1563 2748 4644 5017 1563
Size: 1185 1896 373 3063
Fragments arranged by size:
3063 1896 1185 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2422 2905 3325 4580 5964 6232 1632
Size: 790 483 420 1255 1384 268 1917
Fragments arranged by size:
1917 1384 1255 790 483 420 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6320
AflIII A'CryG_T
Cuts at: 1203 2822 5416 6393 1203
Size: 1619 2594 977 1327
Fragments arranged by size:
2594 1619 1327 977
AgeI A'CCGG_T
Cuts at: 5348 5348
Size: 6517
AhdI GACnn_n'nnGTC
Cuts at: 5246 5246
Size: 6517
AleI CACnn'nnGTG
Cuts at: 1166 3300 1166
Size: 2134 4383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6517
AlwNI CAG_nnn'CTG
Cuts at: 2474 4260 5832 2474
Size: 1786 1572 3159
Fragments arranged by size:
3159 1786 1572
ApaLI G'TGCA_C
Cuts at: 4574 5730 4574
Size: 1156 5361
ApoI r'AATT_y
Cuts at: 1493 2871 3291 3543 3627 3795 3826 4052 4204
Size: 1378 420 252 84 168 31 226 152
Cuts at: 4204 6313 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1378 420 252 226 168 152
84 31
AseI AT'TA_AT
Cuts at: 160 1032 5071 160
Size: 872 4039 1606
Fragments arranged by size:
4039 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
AvaI C'yCGr_G
Cuts at: 1870 1943 3842 1870
Size: 73 1899 4545
Fragments arranged by size:
4545 1899 73
BamHI G'GATC_C
Cuts at: 3847 4214 3847
Size: 367 6150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3838 5293 618
Size: 325 217 627 2051 1455 1842
Fragments arranged by size:
2051 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2685 3211 3836 1391
Size: 1294 526 625 4072
Fragments arranged by size:
4072 1294 625 526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3356 928
Size: 971 1457 4089
Fragments arranged by size:
4089 1457 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4708 4742 1903
Size: 34 2771 34 3678
Fragments arranged by size:
3678 2771 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2160 4409 5619 2160
Size: 2249 1210 3058
Fragments arranged by size:
3058 2249 1210
BclI T'GATC_A
Cuts at: 1548 2641 1548
Size: 1093 5424
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5128 136
Size: 107 122 71 631 605 3456 1525
Fragments arranged by size:
3456 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6512 6512
Size: 6517
BlpI GC'TnA_GC
Cuts at: 3934 3934
Size: 6517
Bme1580I G_kGCm'C
Cuts at: 1790 4578 5734 1790
Size: 2788 1156 2573
Fragments arranged by size:
2788 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6517
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 2513 2973
Size: 416 53 83 399 24 873 223 460
Cuts at: 2973 3057 3141 3393 3729 4248 5206 442
Size: 84 84 252 336 519 958 1753
Fragments arranged by size:
1753 958 873 519 460 416 399 336
252 223 84 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 3237 3489 5177 1239
Size: 899 1099 252 1688 2579
Fragments arranged by size:
2579 1688 1099 899 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2717 4642 5507 5805 2717
Size: 1925 865 298 3429
Fragments arranged by size:
3429 1925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2143 5180 882
Size: 617 644 3037 2219
Fragments arranged by size:
3037 2219 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 4308 493
Size: 711 3104 2702
Fragments arranged by size:
3104 2702 711
BsaBI GATnn'nnATC
Cuts at: 2247 2733 6511 2247
Size: 486 3778 2253
Fragments arranged by size:
3778 2253 486
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 4324 4706 5341
Size: 53 83 186 936 813 1978 382 635
Cuts at: 5341 275
Size: 1451
Fragments arranged by size:
1978 1451 936 813 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4950 5348 5622 5769 6180 4950
Size: 398 274 147 411 5287
Fragments arranged by size:
5287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6292
Fragments arranged by size:
6292 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 5625
BseYI C'CCAG_C
Cuts at: 5720 6216 5720
Size: 496 6021
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5944
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3967 4728 5756 1540
Size: 275 186 1966 761 1028 2301
Fragments arranged by size:
2301 1966 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2372 3211 3325 4578 4663 5734 2372
Size: 839 114 1253 85 1071 3155
Fragments arranged by size:
3155 1253 1071 839 114 85
BsmI GAATG_Cn'
Cuts at: 3080 4028 4121 3080
Size: 948 93 5476
Fragments arranged by size:
5476 948 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2372 2685 3211 3325 3836 4578 4663
Size: 399 582 313 526 114 511 742 85
Cuts at: 4663 5734 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 582 526 511 399 313
114 85
BspEI T'CCGG_A
Cuts at: 6180 6180
Size: 6517
BspHI T'CATG_A
Cuts at: 4406 6136 4406
Size: 1730 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 3872 844
Size: 1590 1438 3489
Fragments arranged by size:
3489 1590 1438
BsrBI CCG'CTC
Cuts at: 1394 1823 4404 1394
Size: 429 2581 3507
Fragments arranged by size:
3507 2581 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5012 5186 65
Size: 1183 3764 174 1396
Fragments arranged by size:
3764 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5161 5348 1700
Size: 110 3351 187 2869
Fragments arranged by size:
3351 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2100 2860 2944 3784 96
Size: 2004 760 84 840 2829
Fragments arranged by size:
2829 2004 840 760 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6517
BssSI C'ACGA_G
Cuts at: 4577 5589 4577
Size: 1012 5505
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6517
BstBI TT'CG_AA
Cuts at: 5321 5321
Size: 6517
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6517
BstYI r'GATC_y
Cuts at: 1972 2232 3847 4214 4599 4616 6068 6154 6166
Size: 260 1615 367 385 17 1452 86 12
Cuts at: 6166 6512 1972
Size: 346 1977
Fragments arranged by size:
1977 1615 1452 385 367 346 260 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 4829
Fragments arranged by size:
4829 1136 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5778
Fragments arranged by size:
5778 683 56
BtsI GCAGTG_nn'
Cuts at: 4033 4827 4847 4033
Size: 794 20 5703
Fragments arranged by size:
5703 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6482
DraI TTT'AAA
Cuts at: 2379 2575 3821 2379
Size: 196 1246 5075
Fragments arranged by size:
5075 1246 196
DraIII CAC_nnn'GTG
Cuts at: 3805 3805
Size: 6517
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5524 809
Size: 507 4208 1802
Fragments arranged by size:
4208 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3964 4853 6482
Size: 54 1425 50 275 18 2134 889 1629
Cuts at: 6482 8
Size: 43
Fragments arranged by size:
2134 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 1537
Size: 275 2152 4090
Fragments arranged by size:
4090 2152 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2270 2441 4447 1353
Size: 837 80 171 2006 3423
Fragments arranged by size:
3423 2006 837 171 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5104 5478 5624 387
Size: 1064 138 3515 374 146 1280
Fragments arranged by size:
3515 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3209 3209
Size: 6517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2422 2905 3237 3325 3489 4580
Size: 393 506 284 483 332 88 164 1091
Cuts at: 4580 5177 5964 6232 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1091 787 597 506 483 393 332
284 268 164 88
EcoNI CCTnn'n_nnAGG
Cuts at: 3343 3343
Size: 6517
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 6517
EcoRI G'AATT_C
Cuts at: 2871 3291 3543 3627 3795 3826 4204 2871
Size: 420 252 84 168 31 378 5184
Fragments arranged by size:
5184 420 378 252 168 84 31
FspI TGC'GCA
Cuts at: 5023 5023
Size: 6517
HaeII r_GCGC'y
Cuts at: 1678 5664 1678
Size: 3986 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 5158 5190 5232
Size: 32 135 32 587 32 2967 32 42
Cuts at: 5232 5264 1373
Size: 32 2626
Fragments arranged by size:
2967 2626 587 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 3861 4107 669
Size: 988 281 520 1403 246 3079
Fragments arranged by size:
3079 1403 988 520 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6517
HpaI GTT'AAC
Cuts at: 2458 4107 2458
Size: 1649 4868
KpnI G_GTAC'C
Cuts at: 3842 3842
Size: 6517
MfeI C'AATT_G
Cuts at: 4116 4116
Size: 6517
MluI A'CGCG_T
Cuts at: 6393 6393
Size: 6517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 5631 5815 1305
Size: 858 3468 184 2007
Fragments arranged by size:
3468 2007 858 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4695
Fragments arranged by size:
4695 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2562 3300 4475 4834 4993 518
Size: 648 1396 738 1175 359 159 2042
Fragments arranged by size:
2042 1396 1175 738 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2404 3190 3933 4610 5758 6003 1606
Size: 798 786 743 677 1148 245 2120
Fragments arranged by size:
2120 1148 798 786 743 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 4829
Fragments arranged by size:
4829 1136 552
NdeI CA'TA_TG
Cuts at: 387 2893 3653 387
Size: 2506 760 3251
Fragments arranged by size:
3251 2506 760
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6517
NotI GC'GGCC_GC
Cuts at: 3964 3964
Size: 6517
NspI r_CATG'y
Cuts at: 1132 1229 2826 2910 3875 5420 1132
Size: 97 1597 84 965 1545 2229
Fragments arranged by size:
2229 1597 1545 965 97 84
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 5848
PciI A'CATG_T
Cuts at: 2822 5416 2822
Size: 2594 3923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6517
PfoI T'CCnGG_A
Cuts at: 3613 3613
Size: 6517
PmeI GTTT'AAAC
Cuts at: 3821 3821
Size: 6517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4586 4618 6099 6131 4586
Size: 32 1481 32 4972
Fragments arranged by size:
4972 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2094 2094
Size: 6517
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6517
PsiI TTA'TAA
Cuts at: 2616 4087 2616
Size: 1471 5046
PstI C_TGCA'G
Cuts at: 830 2409 3869 830
Size: 1579 1460 3478
Fragments arranged by size:
3478 1579 1460
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6517
PvuII CAG'CTG
Cuts at: 2404 3190 2404
Size: 786 5731
SacI G_AGCT'C
Cuts at: 3211 3211
Size: 6517
SalI G'TCGA_C
Cuts at: 1936 3859 1936
Size: 1923 4594
SapI GCTCTTCn'nnn_
Cuts at: 2190 2190
Size: 6517
SbfI CC_TGCA'GG
Cuts at: 3869 3869
Size: 6517
ScaI AGT'ACT
Cuts at: 4765 6477 4765
Size: 1712 4805
SexAI A'CCwGG_T
Cuts at: 2133 2133
Size: 6517
SfcI C'TryA_G
Cuts at: 826 1045 2125 2405 3865 5000 5681 5872 826
Size: 219 1080 280 1460 1135 681 191 1471
Fragments arranged by size:
1471 1460 1135 1080 681 280 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6517
SmaI CCC'GGG
Cuts at: 1872 3844 1872
Size: 1972 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 4621 5522 5784 820
Size: 197 926 753 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 753 262 197
SnaBI TAC'GTA
Cuts at: 493 4308 493
Size: 3815 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6517
SphI G_CATG'C
Cuts at: 1132 2910 3875 1132
Size: 1778 965 3774
Fragments arranged by size:
3774 1778 965
SspI AAT'ATT
Cuts at: 5 52 2514 2596 4441 5
Size: 47 2462 82 1845 2081
Fragments arranged by size:
2462 2081 1845 82 47
StuI AGG'CCT
Cuts at: 1517 3170 3674 3758 1517
Size: 1653 504 84 4276
Fragments arranged by size:
4276 1653 504 84
StyI C'CwwG_G
Cuts at: 513 1065 2201 3956 513
Size: 552 1136 1755 3074
Fragments arranged by size:
3074 1755 1136 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 4544 4561 4714 4899 5344 325
Size: 1255 492 2472 17 153 185 445 1498
Fragments arranged by size:
2472 1498 1255 492 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2100 2860 2944
Size: 274 80 33 51 1443 123 760 84
Cuts at: 2944 3404 3572 3784 4763 6475 96
Size: 460 168 212 979 1712 138
Fragments arranged by size:
1712 1443 979 760 460 274 212 168
138 123 84 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6477 6509 6477
Size: 32 6485
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6517
XbaI T'CTAG_A
Cuts at: 2416 3853 2416
Size: 1437 5080
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6517
XmaI C'CCGG_G
Cuts at: 1870 3842 1870
Size: 1972 4545
XmnI GAAnn'nnTTC
Cuts at: 3086 3254 3590 3758 4646 3086
Size: 168 336 168 888 4957
Fragments arranged by size:
4957 888 336 168 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4325 276
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI