Restriction map for
pFN21AB7823
(Circular) (Six-base) MAPSORT of: pFN21AB7823.seq Check: 796 from: 1 to: 6313
pFN21AB7823 6313 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3263 3263
Size: 6313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3454 3656 1937
Size: 1517 202 4594
Fragments arranged by size:
4594 1517 202
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 2301 3128 4376 5760 6028 1632
Size: 453 216 827 1248 1384 268 1917
Fragments arranged by size:
1917 1384 1248 827 453 268 216
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 3375 5212 6189 1203
Size: 2172 1837 977 1327
Fragments arranged by size:
2172 1837 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6313
AlwNI CAG_nnn'CTG
Cuts at: 3125 4056 5628 3125
Size: 931 1572 3810
Fragments arranged by size:
3810 1572 931
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 2655 2851 3288 3622 3848 4000 6109 1493
Size: 1162 196 437 334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1162 437 334 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 2646 4867 160
Size: 872 1614 2221 1606
Fragments arranged by size:
2221 1614 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 2661 3638 1870
Size: 73 718 977 4545
Fragments arranged by size:
4545 977 718 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2147 2180 2147
Size: 33 6280
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3634 5089 618
Size: 325 217 627 1847 1455 1842
Fragments arranged by size:
1847 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3632 1391
Size: 2241 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2132 2148 2229 4205 5415 2132
Size: 16 81 1976 1210 3030
Fragments arranged by size:
3030 1976 1210 81 16
BclI T'GATC_A
Cuts at: 1548 3304 1548
Size: 1756 4557
BfrBI ATG'CAT
Cuts at: 2695 2695
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2864 4924 136
Size: 107 122 71 631 605 1192 2060 1525
Fragments arranged by size:
2060 1525 1192 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 2923 4374 5530 1790
Size: 1133 1451 1156 2573
Fragments arranged by size:
2573 1451 1156 1133
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2209 3348 4044
Size: 416 53 83 399 24 792 1139 696
Cuts at: 4044 5002 442
Size: 958 1753
Fragments arranged by size:
1753 1139 958 792 696 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2595 3334 4973 1239
Size: 1356 739 1639 2579
Fragments arranged by size:
2579 1639 1356 739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2078 4438 5303 5601 2078
Size: 2360 865 298 2790
Fragments arranged by size:
2790 2360 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2113 4976 882
Size: 617 614 2863 2219
Fragments arranged by size:
2863 2219 617 614
BsaAI yAC'GTr
Cuts at: 493 1204 3053 4104 493
Size: 711 1849 1051 2702
Fragments arranged by size:
2702 1849 1051 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4120 4502 5137 275
Size: 53 83 186 936 2587 382 635 1451
Fragments arranged by size:
2587 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2142 3102 4746 5144 5418 5565 5976 2142
Size: 960 1644 398 274 147 411 2479
Fragments arranged by size:
2479 1644 960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3307 3337 1178
Size: 30 165 30 1904 30 4154
Fragments arranged by size:
4154 1904 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2101 2667 3616 1186
Size: 915 566 949 3883
Fragments arranged by size:
3883 949 915 566
BseYI C'CCAG_C
Cuts at: 3011 5516 6012 3011
Size: 2505 496 3312
Fragments arranged by size:
3312 2505 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2013 1163
Size: 573 277 5463
Fragments arranged by size:
5463 573 277
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4374 4459 5530 4374
Size: 85 1071 5157
Fragments arranged by size:
5157 1071 85
BsiWI C'GTAC_G
Cuts at: 2133 2133
Size: 6313
BsmI GAATG_Cn'
Cuts at: 2260 2386 2564 2695 3824 3917 2260
Size: 126 178 131 1129 93 4656
Fragments arranged by size:
4656 1129 178 131 126 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2923 3632 4374 4459 5530 1391
Size: 399 1133 709 742 85 1071 2174
Fragments arranged by size:
2174 1133 1071 742 709 399 85
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 4202 5932 4202
Size: 1730 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2035 2406 3263 3668 844
Size: 1191 371 857 405 3489
Fragments arranged by size:
3489 1191 857 405 371
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2880 4808 4982 65
Size: 1183 1632 1928 174 1396
Fragments arranged by size:
1928 1632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 2898 3581 4373 5385 2898
Size: 683 792 1012 3826
Fragments arranged by size:
3826 1012 792 683
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6313
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 3256 2272
Size: 984 5329
BstYI r'GATC_y
Cuts at: 1972 2078 2202 2617 3493 3643 4010 4395 4412
Size: 106 124 415 876 150 367 385 17
Cuts at: 4412 5864 5950 5962 6308 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 876 415 385 367 346 150
124 106 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4822
Fragments arranged by size:
4822 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 3829 4623 4643 3829
Size: 794 20 5499
Fragments arranged by size:
5499 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2717 2752 529
Size: 35 2153 35 4090
Fragments arranged by size:
4090 2153 35 35
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 3760 4649
Size: 54 1425 50 275 18 432 1498 889
Cuts at: 4649 6278 8
Size: 1629 43
Fragments arranged by size:
1629 1498 1425 889 432 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2113 2815 2961 3001 3242 3593 4243 1353
Size: 760 702 146 40 241 351 650 3423
Fragments arranged by size:
3423 760 702 650 351 241 146 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4900 5274 5420 387
Size: 1064 138 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2301 2595 3128 3334 4376 4973
Size: 393 453 216 294 533 206 1042 597
Cuts at: 4973 5760 6028 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1042 787 597 533 453 393 294
268 216 206
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
EcoRV GAT'ATC
Cuts at: 3166 3166
Size: 6313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2990 2990
Size: 6313
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 2012 5460 1678
Size: 334 3448 2531
Fragments arranged by size:
3448 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3000 3007 3032 3039 3119
Size: 32 135 32 1428 7 25 7 80
Cuts at: 3119 3151 4954 4986 5028 5060 1373
Size: 32 1803 32 42 32 2626
Fragments arranged by size:
2626 1803 1428 135 80 42 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6313
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 2304 3912 2304
Size: 1608 4705
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 5611 1305
Size: 4122 184 2007
Fragments arranged by size:
4122 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 4059
Fragments arranged by size:
4059 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2844 3585 4271 4630 4789 518
Size: 648 1104 574 741 686 359 159 2042
Fragments arranged by size:
2042 1104 741 686 648 574 359 159
MspA1I CmG'CkG
Cuts at: 1606 2507 3729 4406 5554 5799 1606
Size: 901 1222 677 1148 245 2120
Fragments arranged by size:
2120 1222 1148 901 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4822
Fragments arranged by size:
4822 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NsiI A_TGCA'T
Cuts at: 2697 2697
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 3379 3671 5216 1132
Size: 97 2150 292 1545 2229
Fragments arranged by size:
2229 2150 1545 292 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 3375 5212 3375
Size: 1837 4476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PmlI CAC'GTG
Cuts at: 3053 3053
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2802 1613
Size: 1189 5124
PsiI TTA'TAA
Cuts at: 3294 3883 3294
Size: 589 5724
PstI C_TGCA'G
Cuts at: 830 3665 830
Size: 2835 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 2507 2507
Size: 6313
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SapI GCTCTTCn'nnn_
Cuts at: 3001 3242 3001
Size: 241 6072
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SexAI A'CCwGG_T
Cuts at: 2358 2358
Size: 6313
SfcI C'TryA_G
Cuts at: 826 1045 2828 3661 4796 5477 5668 826
Size: 219 1783 833 1135 681 191 1471
Fragments arranged by size:
1783 1471 1135 833 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 2663 3640 1872
Size: 791 977 4545
Fragments arranged by size:
4545 977 791
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2057 4417 5318 5580 820
Size: 197 926 114 2360 901 262 1553
Fragments arranged by size:
2360 1553 926 901 262 197 114
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6313
StyI C'CwwG_G
Cuts at: 513 1065 2004 2633 3249 3573 3752 513
Size: 552 939 629 616 324 179 3074
Fragments arranged by size:
3074 939 629 616 552 324 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4340 4357 4510 4695 5140 325
Size: 1255 2760 17 153 185 445 1498
Fragments arranged by size:
2760 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2836 4559 6271
Size: 274 80 33 51 1443 859 1723 1712
Cuts at: 6271 96
Size: 138
Fragments arranged by size:
1723 1712 1443 859 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2884 2916 6273 6305 2884
Size: 32 3357 32 2892
Fragments arranged by size:
3357 2892 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2333 1316
Size: 1017 5296
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3361 3361
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 2661 3638 1870
Size: 791 977 4545
Fragments arranged by size:
4545 977 791
XmnI GAAnn'nnTTC
Cuts at: 4442 4442
Size: 6313
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI