Restriction map for
pFN21AB7822
(Circular) (Six-base) MAPSORT of: pFN21AB7822.seq Check: 3918 from: 1 to: 6505
pFN21AB7822 6505 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3220 3220
Size: 6505
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2184 4315 278
Size: 53 83 186 936 648 2131 2468
Fragments arranged by size:
2468 2131 936 648 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3848 1937
Size: 1911 4594
Acc65I G'GTAC_C
Cuts at: 3192 3826 3192
Size: 634 5871
AclI AA'CG_TT
Cuts at: 1563 4632 5005 1563
Size: 3069 373 3063
Fragments arranged by size:
3069 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2405 3569 4568 5952 6220 1632
Size: 773 1164 999 1384 268 1917
Fragments arranged by size:
1917 1384 1164 999 773 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6308
AflIII A'CryG_T
Cuts at: 1203 5404 6381 1203
Size: 4201 977 1327
Fragments arranged by size:
4201 1327 977
AgeI A'CCGG_T
Cuts at: 5336 5336
Size: 6505
AhdI GACnn_n'nnGTC
Cuts at: 5234 5234
Size: 6505
AleI CACnn'nnGTG
Cuts at: 1166 2936 1166
Size: 1770 4735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2350 1184
Size: 1166 5339
AlwNI CAG_nnn'CTG
Cuts at: 2410 2425 2761 3055 4248 5820 2410
Size: 15 336 294 1193 1572 3095
Fragments arranged by size:
3095 1572 1193 336 294 15
ApaLI G'TGCA_C
Cuts at: 3350 4562 5718 3350
Size: 1212 1156 4137
Fragments arranged by size:
4137 1212 1156
ApoI r'AATT_y
Cuts at: 1493 3814 4040 4192 6301 1493
Size: 2321 226 152 2109 1697
Fragments arranged by size:
2321 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5059 160
Size: 872 4027 1606
Fragments arranged by size:
4027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 2701 2990 3108 3201 3636 3830
Size: 73 471 287 289 118 93 435 194
Cuts at: 3830 1870
Size: 4545
Fragments arranged by size:
4545 471 435 289 287 194 118 93
73
BamHI G'GATC_C
Cuts at: 3835 4202 3835
Size: 367 6138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 3192 3598 3826 5281
Size: 325 217 627 1151 254 406 228 1455
Cuts at: 5281 618
Size: 1842
Fragments arranged by size:
1842 1455 1151 627 406 325 254 228
217
BanII G_rGCy'C
Cuts at: 1391 2211 2852 2891 3099 3170 3416 3526 3824
Size: 820 641 39 208 71 246 110 298
Cuts at: 3824 1391
Size: 4072
Fragments arranged by size:
4072 820 641 298 246 208 110 71
39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5534
BbvCI CC'TCA_GC
Cuts at: 3237 3237
Size: 6505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 4696 4730 1903
Size: 34 1793 34 932 34 3678
Fragments arranged by size:
3678 1793 932 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4397 5607 4397
Size: 1210 5295
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2219 2619 5116
Size: 107 122 71 631 605 547 400 2497
Cuts at: 5116 136
Size: 1525
Fragments arranged by size:
2497 1525 631 605 547 400 122 107
71
BglII A'GATC_T
Cuts at: 6500 6500
Size: 6505
BlpI GC'TnA_GC
Cuts at: 3922 3922
Size: 6505
Bme1580I G_kGCm'C
Cuts at: 1790 2898 3354 4566 5722 1790
Size: 1108 456 1212 1156 2573
Fragments arranged by size:
2573 1212 1156 1108 456
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6505
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2997 3011 4236
Size: 416 53 83 399 24 1580 14 1225
Cuts at: 4236 5194 442
Size: 958 1753
Fragments arranged by size:
1753 1580 1225 958 416 399 83 53
24 14
BmtI G_CTAG'C
Cuts at: 1056 2511 1056
Size: 1455 5050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2095 2138 2387 2651 3392 5165 1239
Size: 856 43 249 264 741 1773 2579
Fragments arranged by size:
2579 1773 856 741 264 249 43
Bpu10I CC'TnA_GC
Cuts at: 3237 3473 3237
Size: 236 6269
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3050 4630 5495 5793 2168
Size: 882 1580 865 298 2880
Fragments arranged by size:
2880 1580 882 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2503 5168 882
Size: 617 1004 2665 2219
Fragments arranged by size:
2665 2219 1004 617
BsaAI yAC'GTr
Cuts at: 493 1204 4296 493
Size: 711 3092 2702
Fragments arranged by size:
3092 2702 711
BsaBI GATnn'nnATC
Cuts at: 6499 6499
Size: 6505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2181 3246 4312
Size: 53 83 186 936 488 160 1065 1066
Cuts at: 4312 4694 5329 275
Size: 382 635 1451
Fragments arranged by size:
1451 1066 1065 936 635 488 382 186
160 83 53
BsaWI w'CCGG_w
Cuts at: 2079 3258 4938 5336 5610 5757 6168 2079
Size: 1179 1680 398 274 147 411 2416
Fragments arranged by size:
2416 1680 1179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1999 2029 2681 2711 3350
Size: 30 165 30 596 30 652 30 639
Cuts at: 3350 3380 1178
Size: 30 4303
Fragments arranged by size:
4303 652 639 596 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 3121 3148 3572 1186
Size: 1033 902 27 424 4119
Fragments arranged by size:
4119 1033 902 424 27
BseYI C'CCAG_C
Cuts at: 2434 2897 3088 5708 6204 2434
Size: 463 191 2620 496 2735
Fragments arranged by size:
2735 2620 496 463 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5932
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2342 2687 3955 4716 5744 1540
Size: 275 186 341 345 1268 761 1028 2301
Fragments arranged by size:
2301 1268 1028 761 345 341 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 3354 3566 4566 4651 5722 2211
Size: 1143 212 1000 85 1071 2994
Fragments arranged by size:
2994 1143 1071 1000 212 85
BsiWI C'GTAC_G
Cuts at: 2980 2980
Size: 6505
BsmI GAATG_Cn'
Cuts at: 4016 4109 4016
Size: 93 6412
BsmBI CGTCTCn'nnnn_
Cuts at: 2028 2028
Size: 6505
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2852 2891 2898 3099 3170 3354
Size: 399 421 641 39 7 201 71 184
Cuts at: 3354 3416 3526 3566 3824 4566 4651 5722 1391
Size: 62 110 40 258 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 641 421 399 258 201
184 110 85 71 62 40 39 7
BspEI T'CCGG_A
Cuts at: 2079 6168 2079
Size: 4089 2416
BspHI T'CATG_A
Cuts at: 4394 6124 4394
Size: 1730 4775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3220 3860 844
Size: 2376 640 3489
Fragments arranged by size:
3489 2376 640
BsrBI CCG'CTC
Cuts at: 1394 1823 3271 4392 1394
Size: 429 1448 1121 3507
Fragments arranged by size:
3507 1448 1121 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2401 5000 5174 65
Size: 1183 1153 2599 174 1396
Fragments arranged by size:
2599 1396 1183 1153 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 2260 2306 2337 3477 5149 5336
Size: 110 233 217 46 31 1140 1672 187
Cuts at: 5336 1700
Size: 2869
Fragments arranged by size:
2869 1672 1140 233 217 187 110 46
31
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6505
BssHII G'CGCG_C
Cuts at: 1925 3507 1925
Size: 1582 4923
BssSI C'ACGA_G
Cuts at: 4565 5577 4565
Size: 1012 5493
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2927 794
Size: 2133 4372
BstBI TT'CG_AA
Cuts at: 3420 5309 3420
Size: 1889 4616
BstEII G'GTnAC_C
Cuts at: 1184 2456 1184
Size: 1272 5233
BstXI CCAn_nnnn'nTGG
Cuts at: 2590 3024 2590
Size: 434 6071
BstYI r'GATC_y
Cuts at: 1972 2664 3835 4202 4587 4604 6056 6142 6154
Size: 692 1171 367 385 17 1452 86 12
Cuts at: 6154 6500 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1171 692 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3160 3160
Size: 6505
BtgI C'CryG_G
Cuts at: 513 1065 2909 3615 513
Size: 552 1844 706 3403
Fragments arranged by size:
3403 1844 706 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5766
Fragments arranged by size:
5766 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 3743 4021 4815 4835 2605
Size: 1138 278 794 20 4275
Fragments arranged by size:
4275 1138 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2638 2673 529
Size: 35 2074 35 4361
Fragments arranged by size:
4361 2074 35 35
DraI TTT'AAA
Cuts at: 3809 3809
Size: 6505
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2522 5512 809
Size: 507 1206 2990 1802
Fragments arranged by size:
2990 1802 1206 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2339 2473 3683
Size: 54 1425 50 275 18 509 134 1210
Cuts at: 3683 3952 4841 6470 8
Size: 269 889 1629 43
Fragments arranged by size:
1629 1425 1210 889 509 275 269 134
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2339 3952 1537
Size: 275 527 1613 4090
Fragments arranged by size:
4090 1613 527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2381 3182 4435 1353
Size: 1028 801 1253 3423
Fragments arranged by size:
3423 1253 1028 801
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3155 5092 5466 5612 387
Size: 1064 138 1566 1937 374 146 1280
Fragments arranged by size:
1937 1566 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 2209
Size: 6505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 2138 2387 2405 2651 3392 3569
Size: 393 463 43 249 18 246 741 177
Cuts at: 3569 4568 5165 5952 6220 1239
Size: 999 597 787 268 1524
Fragments arranged by size:
1524 999 787 741 597 463 393 268
249 246 177 43 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3551 3551
Size: 6505
EcoO109I rG'GnC_Cy
Cuts at: 2786 2804 2786
Size: 18 6487
EcoRI G'AATT_C
Cuts at: 3814 4192 3814
Size: 378 6127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2162 3298 2162
Size: 1136 5369
FspI TGC'GCA
Cuts at: 5011 5011
Size: 6505
HaeII r_GCGC'y
Cuts at: 1678 2285 2452 3775 5652 1678
Size: 607 167 1323 1877 2531
Fragments arranged by size:
2531 1877 1323 607 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 3140 3172 3295
Size: 32 135 32 427 32 1109 32 123
Cuts at: 3295 3327 3350 3382 5146 5178 5220 5252 1373
Size: 32 23 32 1764 32 42 32 2626
Fragments arranged by size:
2626 1764 1109 427 135 123 42 32
32 32 32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 3217 3849 4095 669
Size: 988 281 736 543 632 246 3079
Fragments arranged by size:
3079 988 736 632 543 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6505
HpaI GTT'AAC
Cuts at: 3217 4095 3217
Size: 878 5627
KpnI G_GTAC'C
Cuts at: 3196 3830 3196
Size: 634 5871
MfeI C'AATT_G
Cuts at: 4104 4104
Size: 6505
MluI A'CGCG_T
Cuts at: 6381 6381
Size: 6505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5619 5803 1305
Size: 4314 184 2007
Fragments arranged by size:
4314 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4683
Fragments arranged by size:
4683 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 2936 4463 4822 4981 518
Size: 648 1105 665 1527 359 159 2042
Fragments arranged by size:
2042 1527 1105 665 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2422 2761 2911 3052 3586 3921 4598
Size: 406 410 339 150 141 534 335 677
Cuts at: 4598 5746 5991 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 410 406 339 335
245 150 141
NaeI GCC'GGC
Cuts at: 2262 2339 3479 2262
Size: 77 1140 5288
Fragments arranged by size:
5288 1140 77
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5953
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6505
NgoMIV G'CCGG_C
Cuts at: 2260 2337 3477 2260
Size: 77 1140 5288
Fragments arranged by size:
5288 1140 77
NheI G'CTAG_C
Cuts at: 1052 2507 1052
Size: 1455 5050
NotI GC'GGCC_GC
Cuts at: 3952 3952
Size: 6505
NspI r_CATG'y
Cuts at: 1132 1229 2609 3593 3863 5408 1132
Size: 97 1380 984 270 1545 2229
Fragments arranged by size:
2229 1545 1380 984 270 97
PasI CC'CwG_GG
Cuts at: 1348 2480 3518 1348
Size: 1132 1038 4335
Fragments arranged by size:
4335 1132 1038
PciI A'CATG_T
Cuts at: 5404 5404
Size: 6505
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3055 3681 1108
Size: 1947 626 3932
Fragments arranged by size:
3932 1947 626
PfoI T'CCnGG_A
Cuts at: 3368 3368
Size: 6505
PmeI GTTT'AAAC
Cuts at: 3809 3809
Size: 6505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 6087 6119 4574
Size: 32 1481 32 4960
Fragments arranged by size:
4960 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2804 2804
Size: 6505
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6505
PsiI TTA'TAA
Cuts at: 4075 4075
Size: 6505
PspXI vC'TCGA_Gb
Cuts at: 2990 2990
Size: 6505
PstI C_TGCA'G
Cuts at: 830 2055 2757 3857 830
Size: 1225 702 1100 3478
Fragments arranged by size:
3478 1225 1100 702
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6505
PvuII CAG'CTG
Cuts at: 2761 3586 2761
Size: 825 5680
SacI G_AGCT'C
Cuts at: 2211 2211
Size: 6505
SacII CC_GC'GG
Cuts at: 2912 2912
Size: 6505
SalI G'TCGA_C
Cuts at: 1936 3847 1936
Size: 1911 4594
SanDI GG'GwC_CC
Cuts at: 2804 2804
Size: 6505
SapI GCTCTTCn'nnn_
Cuts at: 2381 2381
Size: 6505
SbfI CC_TGCA'GG
Cuts at: 3857 3857
Size: 6505
ScaI AGT'ACT
Cuts at: 4753 6465 4753
Size: 1712 4793
SfcI C'TryA_G
Cuts at: 826 1045 2051 2753 3853 4988 5669 5860 826
Size: 219 1006 702 1100 1135 681 191 1471
Fragments arranged by size:
1471 1135 1100 1006 702 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6505
SmaI CCC'GGG
Cuts at: 1872 2416 2703 3110 3638 3832 1872
Size: 544 287 407 528 194 4545
Fragments arranged by size:
4545 544 528 407 287 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2990 3029 3201 4609 5510
Size: 197 926 204 843 39 172 1408 901
Cuts at: 5510 5772 820
Size: 262 1553
Fragments arranged by size:
1553 1408 926 901 843 262 204 197
172 39
SnaBI TAC'GTA
Cuts at: 493 4296 493
Size: 3803 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6505
SphI G_CATG'C
Cuts at: 1132 2609 3863 1132
Size: 1477 1254 3774
Fragments arranged by size:
3774 1477 1254
SrfI GCCC'GGGC
Cuts at: 2416 2416
Size: 6505
SspI AAT'ATT
Cuts at: 5 52 4429 5
Size: 47 4377 2081
Fragments arranged by size:
4377 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6505
StyI C'CwwG_G
Cuts at: 513 1065 2493 2799 2883 3135 3396 3944 513
Size: 552 1428 306 84 252 261 548 3074
Fragments arranged by size:
3074 1428 552 548 306 261 252 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2380 2493 2506 2896 4532 4549 4702
Size: 1255 800 113 13 390 1636 17 153
Cuts at: 4702 4887 5332 325
Size: 185 445 1498
Fragments arranged by size:
1636 1498 1255 800 445 390 185 153
113 17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4751 6463 96
Size: 274 80 33 51 1443 2774 1712 138
Fragments arranged by size:
2774 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2681 2713 6465 6497 2681
Size: 32 3752 32 2689
Fragments arranged by size:
3752 2689 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6505
XbaI T'CTAG_A
Cuts at: 3841 3841
Size: 6505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3376 3376
Size: 6505
XhoI C'TCGA_G
Cuts at: 1943 2990 3201 1943
Size: 1047 211 5247
Fragments arranged by size:
5247 1047 211
XmaI C'CCGG_G
Cuts at: 1870 2414 2701 3108 3636 3830 1870
Size: 544 287 407 528 194 4545
Fragments arranged by size:
4545 544 528 407 287 194
XmnI GAAnn'nnTTC
Cuts at: 4634 4634
Size: 6505
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2182 4313 276
Size: 53 83 186 936 648 2131 2468
Fragments arranged by size:
2468 2131 936 648 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BstZ17I DraIII EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspOMI PsrI RsrII SexAI
SfiI SfoI SwaI