Restriction map for
pFN21AB7819
(Circular) (Six-base) MAPSORT of: pFN21AB7819.seq Check: 7406 from: 1 to: 6310
pFN21AB7819 6310 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4120 278
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3653 1937
Size: 1716 4594
Acc65I G'GTAC_C
Cuts at: 2598 3631 2598
Size: 1033 5277
AclI AA'CG_TT
Cuts at: 1563 3609 4437 4810 1563
Size: 2046 828 373 3063
Fragments arranged by size:
3063 2046 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3600 4373 5757 6025 1632
Size: 1968 773 1384 268 1917
Fragments arranged by size:
1968 1917 1384 773 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6113
AflIII A'CryG_T
Cuts at: 1203 5209 6186 1203
Size: 4006 977 1327
Fragments arranged by size:
4006 1327 977
AgeI A'CCGG_T
Cuts at: 5141 5141
Size: 6310
AhdI GACnn_n'nnGTC
Cuts at: 5039 5039
Size: 6310
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2633 1184
Size: 1449 4861
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 6278
AlwNI CAG_nnn'CTG
Cuts at: 4053 5625 4053
Size: 1572 4738
ApaI G_GGCC'C
Cuts at: 3172 3249 3335 3556 3172
Size: 77 86 221 5926
Fragments arranged by size:
5926 221 86 77
ApaLI G'TGCA_C
Cuts at: 4367 5523 4367
Size: 1156 5154
ApoI r'AATT_y
Cuts at: 1493 3619 3845 3997 6106 1493
Size: 2126 226 152 2109 1697
Fragments arranged by size:
2126 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4864 160
Size: 872 3832 1606
Fragments arranged by size:
3832 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
AvaI C'yCGr_G
Cuts at: 1870 1943 2179 2359 3174 3635 1870
Size: 73 236 180 815 461 4545
Fragments arranged by size:
4545 815 461 236 180 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3299 3332 3299
Size: 33 6277
BamHI G'GATC_C
Cuts at: 3640 4007 3640
Size: 367 5943
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2080 2087 2186 2194
Size: 325 217 627 258 35 7 99 8
Cuts at: 2194 2598 3283 3307 3631 5086 618
Size: 404 685 24 324 1455 1842
Fragments arranged by size:
1842 1455 685 627 404 325 324 258
217 99 35 24 8 7
BanII G_rGCy'C
Cuts at: 1391 2355 2541 3172 3249 3335 3556 3629 1391
Size: 964 186 631 77 86 221 73 4072
Fragments arranged by size:
4072 964 631 221 186 86 77 73
BbeI G_GCGC'C
Cuts at: 2084 2091 2190 2084
Size: 7 99 6204
Fragments arranged by size:
6204 99 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4501 4535 1903
Size: 34 2564 34 3678
Fragments arranged by size:
3678 2564 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3102 4202 5412 3102
Size: 1100 1210 4000
Fragments arranged by size:
4000 1210 1100
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3387 4921 136
Size: 107 122 71 631 605 1715 1534 1525
Fragments arranged by size:
1715 1534 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6305 6305
Size: 6310
BlpI GC'TnA_GC
Cuts at: 3727 3727
Size: 6310
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2199 3172 3249 3335 3556 4371 5527
Size: 258 151 973 77 86 221 815 1156
Cuts at: 5527 1790
Size: 2573
Fragments arranged by size:
2573 1156 973 815 258 221 151 86
77
BmgBI CAC'GTC
Cuts at: 1318 2569 1318
Size: 1251 5059
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2890 3307 3488
Size: 416 53 83 399 24 1473 417 181
Cuts at: 3488 4041 4999 442
Size: 553 958 1753
Fragments arranged by size:
1753 1473 958 553 417 416 399 181
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2555 2768 3309 3405 4970 1239
Size: 1178 138 213 541 96 1565 2579
Fragments arranged by size:
2579 1565 1178 541 213 138 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4435 5300 5598 4435
Size: 865 298 5147
Fragments arranged by size:
5147 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2175 4973 882
Size: 617 676 2798 2219
Fragments arranged by size:
2798 2219 676 617
BsaAI yAC'GTr
Cuts at: 493 1204 4101 493
Size: 711 2897 2702
Fragments arranged by size:
2897 2702 711
BsaBI GATnn'nnATC
Cuts at: 2477 6304 2477
Size: 3827 2483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2081 2088 2187 2298
Size: 53 83 186 936 548 7 99 111
Cuts at: 2298 2753 4117 4499 5134 275
Size: 455 1364 382 635 1451
Fragments arranged by size:
1451 1364 936 635 548 455 382 186
111 99 83 53 7
BsaWI w'CCGG_w
Cuts at: 4743 5141 5415 5562 5973 4743
Size: 398 274 147 411 5080
Fragments arranged by size:
5080 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2660 2690 3524 3554 1178
Size: 30 165 30 1257 30 834 30 3934
Fragments arranged by size:
3934 1257 834 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3063 3514 3529 1186
Size: 1877 451 15 3967
Fragments arranged by size:
3967 1877 451 15
BseYI C'CCAG_C
Cuts at: 3101 3163 3548 5513 6009 3101
Size: 62 385 1965 496 3402
Fragments arranged by size:
3402 1965 496 385 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2639 1163
Size: 573 903 4834
Fragments arranged by size:
4834 903 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2256 3458 3760 4521 5549 1540
Size: 275 186 255 1202 302 761 1028 2301
Fragments arranged by size:
2301 1202 1028 761 302 275 255 186
BsiHKAI G_wGCw'C
Cuts at: 2784 4371 4456 5527 2784
Size: 1587 85 1071 3567
Fragments arranged by size:
3567 1587 1071 85
BsmI GAATG_Cn'
Cuts at: 2929 3224 3821 3914 2929
Size: 295 597 93 5325
Fragments arranged by size:
5325 597 295 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2199 2355 2541 2784 3172 3249
Size: 399 258 151 156 186 243 388 77
Cuts at: 3249 3335 3556 3629 4371 4456 5527 1391
Size: 86 221 73 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 258 243 221
186 156 151 86 85 77 73
BspEI T'CCGG_A
Cuts at: 5973 5973
Size: 6310
BspHI T'CATG_A
Cuts at: 4199 5929 4199
Size: 1730 4580
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3665 844
Size: 2821 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2607 4197 1394
Size: 429 784 1590 3507
Fragments arranged by size:
3507 1590 784 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2901 4805 4979 65
Size: 1183 1653 1904 174 1396
Fragments arranged by size:
1904 1653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 4954 5141 1700
Size: 110 445 2699 187 2869
Fragments arranged by size:
2869 2699 445 187 110
BsrGI T'GTAC_A
Cuts at: 96 2997 96
Size: 2901 3409
BssHII G'CGCG_C
Cuts at: 1925 2127 2344 1925
Size: 202 217 5891
Fragments arranged by size:
5891 217 202
BssSI C'ACGA_G
Cuts at: 4370 5382 4370
Size: 1012 5298
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2701 794
Size: 1907 4403
BstBI TT'CG_AA
Cuts at: 5114 5114
Size: 6310
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6310
BstYI r'GATC_y
Cuts at: 1972 3640 4007 4392 4409 5861 5947 5959 6305
Size: 1668 367 385 17 1452 86 12 346
Cuts at: 6305 1972
Size: 1977
Fragments arranged by size:
1977 1668 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2292 2388 3369 513
Size: 552 939 288 96 981 3454
Fragments arranged by size:
3454 981 939 552 288 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5571
Fragments arranged by size:
5571 683 56
BtsI GCAGTG_nn'
Cuts at: 2627 3826 4620 4640 2627
Size: 1199 794 20 4297
Fragments arranged by size:
4297 1199 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6275
DraI TTT'AAA
Cuts at: 3614 3614
Size: 6310
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3042 5317 809
Size: 507 1726 2275 1802
Fragments arranged by size:
2275 1802 1726 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2526 3127
Size: 54 1425 50 275 18 423 273 601
Cuts at: 3127 3336 3455 3757 4646 6275 8
Size: 209 119 302 889 1629 43
Fragments arranged by size:
1629 1425 889 601 423 302 275 273
209 119 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2253 3455 3757 1537
Size: 275 441 1202 302 4090
Fragments arranged by size:
4090 1202 441 302 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4240 1353
Size: 2887 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2512 3338 4897 5271 5417 387
Size: 1064 138 923 826 1559 374 146 1280
Fragments arranged by size:
1559 1280 1064 923 826 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2555 2768 3309 3405 3600 4373
Size: 393 785 138 213 541 96 195 773
Cuts at: 4373 4970 5757 6025 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 785 773 597 541 393 268
213 195 138 96
EcoO109I rG'GnC_Cy
Cuts at: 3168 3169 3277 3419 3552 3553 3168
Size: 1 108 142 133 1 5925
Fragments arranged by size:
5925 142 133 108 1 1
EcoRI G'AATT_C
Cuts at: 3619 3997 3619
Size: 378 5932
FspI TGC'GCA
Cuts at: 4816 4816
Size: 6310
HaeII r_GCGC'y
Cuts at: 1678 2084 2091 2190 5457 1678
Size: 406 7 99 3267 2531
Fragments arranged by size:
3267 2531 406 99 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3053 3085 3524 3556 4951
Size: 32 135 32 1481 32 439 32 1395
Cuts at: 4951 4983 5025 5057 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1481 1395 439 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3010 3654 3900 669
Size: 988 281 1072 644 246 3079
Fragments arranged by size:
3079 1072 988 644 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6310
HpaI GTT'AAC
Cuts at: 3900 3900
Size: 6310
KasI G'GCGC_C
Cuts at: 2080 2087 2186 2080
Size: 7 99 6204
Fragments arranged by size:
6204 99 7
KpnI G_GTAC'C
Cuts at: 2602 3635 2602
Size: 1033 5277
MfeI C'AATT_G
Cuts at: 3909 3909
Size: 6310
MluI A'CGCG_T
Cuts at: 6186 6186
Size: 6310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2781 2889 5424 5608 1305
Size: 1476 108 2535 184 2007
Fragments arranged by size:
2535 2007 1476 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3129 10
Size: 54 1425 343 1297 3191
Fragments arranged by size:
3191 1425 1297 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2906 3186 4268 4627 4786 518
Size: 648 1740 280 1082 359 159 2042
Fragments arranged by size:
2042 1740 1082 648 359 280 159
MspA1I CmG'CkG
Cuts at: 1606 2294 2390 2941 3342 3726 4403 5551 5796
Size: 688 96 551 401 384 677 1148 245
Cuts at: 5796 1606
Size: 2120
Fragments arranged by size:
2120 1148 688 677 551 401 384 245
96
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 6310
NarI GG'CG_CC
Cuts at: 2081 2088 2187 2081
Size: 7 99 6204
Fragments arranged by size:
6204 99 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 3369 513
Size: 552 939 1365 3454
Fragments arranged by size:
3454 1365 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6310
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 6310
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6310
NotI GC'GGCC_GC
Cuts at: 3757 3757
Size: 6310
NspI r_CATG'y
Cuts at: 1132 1229 2038 2647 3668 5213 1132
Size: 97 809 609 1021 1545 2229
Fragments arranged by size:
2229 1545 1021 809 609 97
PasI CC'CwG_GG
Cuts at: 1348 3242 1348
Size: 1894 4416
PciI A'CATG_T
Cuts at: 5209 5209
Size: 6310
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6310
PfoI T'CCnGG_A
Cuts at: 2976 2976
Size: 6310
PmeI GTTT'AAAC
Cuts at: 3614 3614
Size: 6310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4379 4411 5892 5924 4379
Size: 32 1481 32 4765
Fragments arranged by size:
4765 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3277 3419 3277
Size: 142 6168
PshAI GACnn'nnGTC
Cuts at: 1613 2145 3417 1613
Size: 532 1272 4506
Fragments arranged by size:
4506 1272 532
PsiI TTA'TAA
Cuts at: 3594 3880 3594
Size: 286 6024
PspOMI G'GGCC_C
Cuts at: 3168 3245 3331 3552 3168
Size: 77 86 221 5926
Fragments arranged by size:
5926 221 86 77
PstI C_TGCA'G
Cuts at: 830 3662 830
Size: 2832 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6310
PvuII CAG'CTG
Cuts at: 2941 3342 2941
Size: 401 5909
SacII CC_GC'GG
Cuts at: 2295 2391 2295
Size: 96 6214
SalI G'TCGA_C
Cuts at: 1936 3652 1936
Size: 1716 4594
SanDI GG'GwC_CC
Cuts at: 3419 3419
Size: 6310
SbfI CC_TGCA'GG
Cuts at: 3662 3662
Size: 6310
ScaI AGT'ACT
Cuts at: 4558 6270 4558
Size: 1712 4598
SfcI C'TryA_G
Cuts at: 826 1045 2551 3658 4793 5474 5665 826
Size: 219 1506 1107 1135 681 191 1471
Fragments arranged by size:
1506 1471 1135 1107 681 219 191
SfoI GGC'GCC
Cuts at: 2082 2089 2188 2082
Size: 7 99 6204
Fragments arranged by size:
6204 99 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6310
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6310
SmaI CCC'GGG
Cuts at: 1872 2181 2361 3176 3637 1872
Size: 309 180 815 461 4545
Fragments arranged by size:
4545 815 461 309 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4414 5315 5577 820
Size: 197 926 2471 901 262 1553
Fragments arranged by size:
2471 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4101 493
Size: 3608 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6310
SphI G_CATG'C
Cuts at: 1132 2647 3668 1132
Size: 1515 1021 3774
Fragments arranged by size:
3774 1515 1021
SspI AAT'ATT
Cuts at: 5 52 4234 5
Size: 47 4182 2081
Fragments arranged by size:
4182 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6310
StyI C'CwwG_G
Cuts at: 513 1065 2004 2190 2218 3052 3369 3426 3749
Size: 552 939 186 28 834 317 57 323
Cuts at: 3749 513
Size: 3074
Fragments arranged by size:
3074 939 834 552 323 317 186 57
28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 4337 4354 4507 4692 5137 325
Size: 1255 1025 1732 17 153 185 445 1498
Fragments arranged by size:
1732 1498 1255 1025 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2997 4556 6268
Size: 274 80 33 51 1443 1020 1559 1712
Cuts at: 6268 96
Size: 138
Fragments arranged by size:
1712 1559 1443 1020 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2660 2692 6270 6302 2660
Size: 32 3578 32 2668
Fragments arranged by size:
3578 2668 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2570 1316
Size: 1254 5056
XbaI T'CTAG_A
Cuts at: 3646 3646
Size: 6310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6310
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6310
XmaI C'CCGG_G
Cuts at: 1870 2179 2359 3174 3635 1870
Size: 309 180 815 461 4545
Fragments arranged by size:
4545 815 461 309 180
XmnI GAAnn'nnTTC
Cuts at: 4439 4439
Size: 6310
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4118 276
Size: 53 83 186 936 2584 2468
Fragments arranged by size:
2584 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI
SwaI