Restriction map for
pFN21AB7818
(Circular) (Six-base) MAPSORT of: pFN21AB7818.seq Check: 7979 from: 1 to: 6499
pFN21AB7818 6499 bp
With 182 enzymes: SgfI *
February 20, 2021 22:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2328 2734 2328
Size: 406 6093
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2223 4309 278
Size: 53 83 186 936 687 2086 2468
Fragments arranged by size:
2468 2086 936 687 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3123 3842 1937
Size: 1186 719 4594
Fragments arranged by size:
4594 1186 719
Acc65I G'GTAC_C
Cuts at: 3820 3820
Size: 6499
AclI AA'CG_TT
Cuts at: 1563 4626 4999 1563
Size: 3063 373 3063
Fragments arranged by size:
3063 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 4562 5946 6214 1632
Size: 535 2395 1384 268 1917
Fragments arranged by size:
2395 1917 1384 535 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6302
AflIII A'CryG_T
Cuts at: 1203 2721 2895 5398 6375 1203
Size: 1518 174 2503 977 1327
Fragments arranged by size:
2503 1518 1327 977 174
AgeI A'CCGG_T
Cuts at: 5330 5330
Size: 6499
AhdI GACnn_n'nnGTC
Cuts at: 5228 5228
Size: 6499
AleI CACnn'nnGTG
Cuts at: 1166 2561 2744 1166
Size: 1395 183 4921
Fragments arranged by size:
4921 1395 183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2901 1184
Size: 1717 4782
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3330 3362 3330
Size: 32 6467
AlwNI CAG_nnn'CTG
Cuts at: 4242 5814 4242
Size: 1572 4927
ApaI G_GGCC'C
Cuts at: 2595 3118 2595
Size: 523 5976
ApaLI G'TGCA_C
Cuts at: 4556 5712 4556
Size: 1156 5343
ApoI r'AATT_y
Cuts at: 1493 3467 3808 4034 4186 6295 1493
Size: 1974 341 226 152 2109 1697
Fragments arranged by size:
2109 1974 1697 341 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5053 160
Size: 872 4021 1606
Fragments arranged by size:
4021 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
AvaI C'yCGr_G
Cuts at: 1870 1943 2401 2440 2701 3824 1870
Size: 73 458 39 261 1123 4545
Fragments arranged by size:
4545 1123 458 261 73 39
BamHI G'GATC_C
Cuts at: 3829 4196 3829
Size: 367 6132
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 2746 3748 3820 5275
Size: 325 217 627 768 191 1002 72 1455
Cuts at: 5275 618
Size: 1842
Fragments arranged by size:
1842 1455 1002 768 627 325 217 191
72
BanII G_rGCy'C
Cuts at: 1391 2595 2622 2771 3059 3069 3118 3558 3818
Size: 1204 27 149 288 10 49 440 260
Cuts at: 3818 1391
Size: 4072
Fragments arranged by size:
4072 1204 440 288 260 149 49 27
10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2289 3747 928
Size: 971 390 1458 3680
Fragments arranged by size:
3680 1458 971 390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3413 3447 3488 3522 4690
Size: 34 50 34 1392 34 41 34 1168
Cuts at: 4690 4724 1903
Size: 34 3678
Fragments arranged by size:
3678 1392 1168 50 41 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4391 5601 4391
Size: 1210 5289
BclI T'GATC_A
Cuts at: 1548 3096 1548
Size: 1548 4951
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5110 136
Size: 107 122 71 631 605 3438 1525
Fragments arranged by size:
3438 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6494 6494
Size: 6499
BlpI GC'TnA_GC
Cuts at: 3916 3916
Size: 6499
Bme1580I G_kGCm'C
Cuts at: 1790 2595 2667 2751 3106 3118 4560 5716 1790
Size: 805 72 84 355 12 1442 1156 2573
Fragments arranged by size:
2573 1442 1156 805 355 84 72 12
BmgBI CAC'GTC
Cuts at: 1318 2602 1318
Size: 1284 5215
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 2915 3368
Size: 416 53 83 399 24 906 592 453
Cuts at: 3368 4230 5188 442
Size: 862 958 1753
Fragments arranged by size:
1753 958 906 862 592 453 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 5159 1239
Size: 858 3062 2579
Fragments arranged by size:
3062 2579 858
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2025 3542 4624 5489 5787 2025
Size: 1517 1082 865 298 2737
Fragments arranged by size:
2737 1517 1082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5162 882
Size: 617 3663 2219
Fragments arranged by size:
3663 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2473 2638 2896 3619 4290 493
Size: 711 1269 165 258 723 671 2702
Fragments arranged by size:
2702 1269 723 711 671 258 165
BsaBI GATnn'nnATC
Cuts at: 6493 6493
Size: 6499
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2220 2390 4306 4688
Size: 53 83 186 936 687 170 1916 382
Cuts at: 4688 5323 275
Size: 635 1451
Fragments arranged by size:
1916 1451 936 687 635 382 186 170
83 53
BsaWI w'CCGG_w
Cuts at: 2255 4932 5330 5604 5751 6162 2255
Size: 2677 398 274 147 411 2592
Fragments arranged by size:
2677 2592 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2360 2390 3019 3049 1178
Size: 30 165 30 957 30 629 30 4628
Fragments arranged by size:
4628 957 629 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2368 3463 1186
Size: 1165 17 1095 4222
Fragments arranged by size:
4222 1165 1095 17
BseYI C'CCAG_C
Cuts at: 2177 3204 3261 5702 6198 2177
Size: 1027 57 2441 496 2478
Fragments arranged by size:
2478 2441 1027 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 2582 1163
Size: 573 699 147 5080
Fragments arranged by size:
5080 699 573 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3949 4710 5738 1540
Size: 275 186 1948 761 1028 2301
Fragments arranged by size:
2301 1948 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4560 4645 5716 4560
Size: 85 1071 5343
Fragments arranged by size:
5343 1071 85
BsmI GAATG_Cn'
Cuts at: 4010 4103 4010
Size: 93 6406
BsmBI CGTCTCn'nnnn_
Cuts at: 2730 2730
Size: 6499
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2595 2622 2667 2751 2771 3059 3069
Size: 399 805 27 45 84 20 288 10
Cuts at: 3069 3106 3118 3558 3818 4560 4645 5716 1391
Size: 37 12 440 260 742 85 1071 2174
Fragments arranged by size:
2174 1071 805 742 440 399 288 260
85 84 45 37 27 20 12 10
BspEI T'CCGG_A
Cuts at: 2255 6162 2255
Size: 3907 2592
BspHI T'CATG_A
Cuts at: 4388 6118 4388
Size: 1730 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2328 2734 3300 3321 3854 844
Size: 1484 406 566 21 533 3489
Fragments arranged by size:
3489 1484 566 533 406 21
BsrBI CCG'CTC
Cuts at: 1394 1823 4386 1394
Size: 429 2563 3507
Fragments arranged by size:
3507 2563 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3406 3431 3519 4994 5168 65
Size: 1183 2158 25 88 1475 174 1396
Fragments arranged by size:
2158 1475 1396 1183 174 88 25
BsrFI r'CCGG_y
Cuts at: 1700 1810 2624 5143 5330 1700
Size: 110 814 2519 187 2869
Fragments arranged by size:
2869 2519 814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6499
BssSI C'ACGA_G
Cuts at: 4559 5571 4559
Size: 1012 5487
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3421 794
Size: 2627 3872
BstBI TT'CG_AA
Cuts at: 5303 5303
Size: 6499
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6499
BstXI CCAn_nnnn'nTGG
Cuts at: 3376 3574 3670 3376
Size: 198 96 6205
Fragments arranged by size:
6205 198 96
BstYI r'GATC_y
Cuts at: 1972 2444 3273 3829 4196 4581 4598 6050 6136
Size: 472 829 556 367 385 17 1452 86
Cuts at: 6136 6148 6494 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 829 556 472 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2691 3243 513
Size: 552 1626 552 3769
Fragments arranged by size:
3769 1626 552 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5760
Fragments arranged by size:
5760 683 56
BtsI GCAGTG_nn'
Cuts at: 2157 4015 4809 4829 2157
Size: 1858 794 20 3827
Fragments arranged by size:
3827 1858 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2217 2252 529
Size: 35 1653 35 4776
Fragments arranged by size:
4776 1653 35 35
DraI TTT'AAA
Cuts at: 3018 3803 3018
Size: 785 5714
DraIII CAC_nnn'GTG
Cuts at: 2638 2641 2638
Size: 3 6496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5506 809
Size: 507 4190 1802
Fragments arranged by size:
4190 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2475 2716 3946
Size: 54 1425 50 275 18 645 241 1230
Cuts at: 3946 4835 6464 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1230 889 645 275 241 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3946 1537
Size: 275 2134 4090
Fragments arranged by size:
4090 2134 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2086 2534 3047 4429 1353
Size: 733 448 513 1382 3423
Fragments arranged by size:
3423 1382 733 513 448
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5086 5460 5606 387
Size: 1064 138 3497 374 146 1280
Fragments arranged by size:
3497 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2167 4562 5159 5946 6214 1239
Size: 393 465 70 2395 597 787 268 1524
Fragments arranged by size:
2395 1524 787 597 465 393 268 70
EcoO109I rG'GnC_Cy
Cuts at: 2031 2592 3114 2031
Size: 561 522 5416
Fragments arranged by size:
5416 561 522
EcoRI G'AATT_C
Cuts at: 3467 3808 4186 3467
Size: 341 378 5780
Fragments arranged by size:
5780 378 341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3332 3332
Size: 6499
FspI TGC'GCA
Cuts at: 2837 5005 2837
Size: 2168 4331
FspAI rTGC'GCAy
Cuts at: 2837 2837
Size: 6499
HaeII r_GCGC'y
Cuts at: 1678 5646 1678
Size: 3968 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 3540 3572 5140
Size: 32 135 32 857 32 1079 32 1568
Cuts at: 5140 5172 5214 5246 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1568 1079 857 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3124 3843 4089 669
Size: 988 281 1186 719 246 3079
Fragments arranged by size:
3079 1186 988 719 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6499
HpaI GTT'AAC
Cuts at: 4089 4089
Size: 6499
KpnI G_GTAC'C
Cuts at: 3824 3824
Size: 6499
MfeI C'AATT_G
Cuts at: 4098 4098
Size: 6499
MluI A'CGCG_T
Cuts at: 2721 6375 2721
Size: 3654 2845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2802 3153 3393 3501 5613 5797 1305
Size: 1497 351 240 108 2112 184 2007
Fragments arranged by size:
2112 2007 1497 351 240 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 10
Size: 54 1425 343 645 4032
Fragments arranged by size:
4032 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2561 2744 4457 4816 4975 518
Size: 648 1125 270 183 1713 359 159 2042
Fragments arranged by size:
2042 1713 1125 648 359 270 183 159
MspA1I CmG'CkG
Cuts at: 1606 2731 3915 4592 5740 5985 1606
Size: 1125 1184 677 1148 245 2120
Fragments arranged by size:
2120 1184 1148 1125 677 245
NaeI GCC'GGC
Cuts at: 2626 2626
Size: 6499
NcoI C'CATG_G
Cuts at: 513 1065 2691 3243 513
Size: 552 1626 552 3769
Fragments arranged by size:
3769 1626 552 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6499
NgoMIV G'CCGG_C
Cuts at: 2624 2624
Size: 6499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6499
NotI GC'GGCC_GC
Cuts at: 3946 3946
Size: 6499
NspI r_CATG'y
Cuts at: 1132 1229 3857 5402 1132
Size: 97 2628 1545 2229
Fragments arranged by size:
2628 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6499
PciI A'CATG_T
Cuts at: 5398 5398
Size: 6499
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2638 2641 1108
Size: 1530 3 4966
Fragments arranged by size:
4966 1530 3
PfoI T'CCnGG_A
Cuts at: 3780 3780
Size: 6499
PmeI GTTT'AAAC
Cuts at: 3803 3803
Size: 6499
PmlI CAC'GTG
Cuts at: 2638 3619 2638
Size: 981 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4568 4600 6081 6113 4568
Size: 32 1481 32 4954
Fragments arranged by size:
4954 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2031 2031
Size: 6499
PshAI GACnn'nnGTC
Cuts at: 1613 2263 2931 1613
Size: 650 668 5181
Fragments arranged by size:
5181 668 650
PsiI TTA'TAA
Cuts at: 4069 4069
Size: 6499
PspOMI G'GGCC_C
Cuts at: 2591 3114 2591
Size: 523 5976
PstI C_TGCA'G
Cuts at: 830 2712 3851 830
Size: 1882 1139 3478
Fragments arranged by size:
3478 1882 1139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6499
PvuII CAG'CTG
Cuts at: 2731 2731
Size: 6499
SalI G'TCGA_C
Cuts at: 1936 3122 3841 1936
Size: 1186 719 4594
Fragments arranged by size:
4594 1186 719
SapI GCTCTTCn'nnn_
Cuts at: 3047 3047
Size: 6499
SbfI CC_TGCA'GG
Cuts at: 3851 3851
Size: 6499
ScaI AGT'ACT
Cuts at: 2954 4747 6459 2954
Size: 1793 1712 2994
Fragments arranged by size:
2994 1793 1712
SfcI C'TryA_G
Cuts at: 826 1045 2068 2203 2708 3713 3847 4982 5663
Size: 219 1023 135 505 1005 134 1135 681
Cuts at: 5663 5854 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1023 1005 681 505 219 191
135 134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6499
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6499
SmaI CCC'GGG
Cuts at: 1872 2403 2442 2703 3826 1872
Size: 531 39 261 1123 4545
Fragments arranged by size:
4545 1123 531 261 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2040 3557 4603 5504 5766 820
Size: 197 926 97 1517 1046 901 262 1553
Fragments arranged by size:
1553 1517 1046 926 901 262 197 97
SnaBI TAC'GTA
Cuts at: 493 4290 493
Size: 3797 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6499
SphI G_CATG'C
Cuts at: 1132 3857 1132
Size: 2725 3774
SspI AAT'ATT
Cuts at: 5 52 4423 5
Size: 47 4371 2081
Fragments arranged by size:
4371 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6499
StyI C'CwwG_G
Cuts at: 513 1065 2691 3243 3938 513
Size: 552 1626 552 695 3074
Fragments arranged by size:
3074 1626 695 552 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3218 4526 4543 4696 4881 5326 325
Size: 1255 1638 1308 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1308 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2952 4745 6457
Size: 274 80 33 51 1443 975 1793 1712
Cuts at: 6457 96
Size: 138
Fragments arranged by size:
1793 1712 1443 975 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6459 6491 6459
Size: 32 6467
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6499
XbaI T'CTAG_A
Cuts at: 3835 3835
Size: 6499
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6499
XmaI C'CCGG_G
Cuts at: 1870 2401 2440 2701 3824 1870
Size: 531 39 261 1123 4545
Fragments arranged by size:
4545 1123 531 261 39
XmnI GAAnn'nnTTC
Cuts at: 2286 3000 4628 2286
Size: 714 1628 4157
Fragments arranged by size:
4157 1628 714
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2221 4307 276
Size: 53 83 186 936 687 2086 2468
Fragments arranged by size:
2468 2086 936 687 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI