Restriction map for
pFN21AB7816
(Circular) (Six-base) MAPSORT of: pFN21AB7816.seq Check: 6033 from: 1 to: 6493
pFN21AB7816 6493 bp
With 182 enzymes: SgfI *
March 28, 2012 12:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3836 1937
Size: 1899 4594
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3486 3488 4556 5940 6208 1632
Size: 1854 2 1068 1384 268 1917
Fragments arranged by size:
1917 1854 1384 1068 268 2
AfeI AGC'GCT
Cuts at: 3764 3764
Size: 6493
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 2833 5392 6369 1203
Size: 1630 2559 977 1327
Fragments arranged by size:
2559 1630 1327 977
AgeI A'CCGG_T
Cuts at: 2580 5324 2580
Size: 2744 3749
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2297 3373 1184
Size: 1113 1076 4304
Fragments arranged by size:
4304 1113 1076
AlwNI CAG_nnn'CTG
Cuts at: 2467 2564 4236 5808 2467
Size: 97 1672 1572 3152
Fragments arranged by size:
3152 1672 1572 97
ApaI G_GGCC'C
Cuts at: 2538 2538
Size: 6493
ApaLI G'TGCA_C
Cuts at: 2378 4550 5706 2378
Size: 2172 1156 3165
Fragments arranged by size:
3165 2172 1156
ApoI r'AATT_y
Cuts at: 1493 3056 3802 4028 4180 6289 1493
Size: 1563 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 1563 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 2210 2543 3818 1870
Size: 73 222 45 333 1275 4545
Fragments arranged by size:
4545 1275 333 222 73 45
BamHI G'GATC_C
Cuts at: 3823 4190 3823
Size: 367 6126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2061 2434 3814 5269 618
Size: 325 217 627 274 373 1380 1455 1842
Fragments arranged by size:
1842 1455 1380 627 373 325 274 217
BanII G_rGCy'C
Cuts at: 1391 2211 2217 2538 3812 1391
Size: 820 6 321 1274 4072
Fragments arranged by size:
4072 1274 820 321 6
BbeI G_GCGC'C
Cuts at: 2065 2065
Size: 6493
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3660 928
Size: 971 1761 3761
Fragments arranged by size:
3761 1761 971
BbvCI CC'TCA_GC
Cuts at: 2896 2896
Size: 6493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2889 2923 4684 4718 1903
Size: 34 952 34 1761 34 3678
Fragments arranged by size:
3678 1761 952 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6493
BfrBI ATG'CAT
Cuts at: 2716 2716
Size: 6493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2408 2417
Size: 107 122 71 631 605 337 399 9
Cuts at: 2417 2533 5104 136
Size: 116 2571 1525
Fragments arranged by size:
2571 1525 631 605 399 337 122 116
107 71 9
BglII A'GATC_T
Cuts at: 6488 6488
Size: 6493
BlpI GC'TnA_GC
Cuts at: 2156 3405 3910 2156
Size: 1249 505 4739
Fragments arranged by size:
4739 1249 505
Bme1580I G_kGCm'C
Cuts at: 1790 2382 2439 2538 3206 4554 5710 1790
Size: 592 57 99 668 1348 1156 2573
Fragments arranged by size:
2573 1348 1156 668 592 99 57
BmgBI CAC'GTC
Cuts at: 1318 3745 1318
Size: 2427 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2672 4224 5182
Size: 416 53 83 399 24 1255 1552 958
Cuts at: 5182 442
Size: 1753
Fragments arranged by size:
1753 1552 1255 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3285 3642 1056
Size: 2229 357 3907
Fragments arranged by size:
3907 2229 357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2762 3311 3657 5153 1239
Size: 1211 312 549 346 1496 2579
Fragments arranged by size:
2579 1496 1211 549 346 312
Bpu10I CC'TnA_GC
Cuts at: 2896 2896
Size: 6493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 4618 5483 5781 2369
Size: 2249 865 298 3081
Fragments arranged by size:
3081 2249 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5156 882
Size: 617 3657 2219
Fragments arranged by size:
3657 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3581 4284 493
Size: 711 2377 703 2702
Fragments arranged by size:
2702 2377 711 703
BsaBI GATnn'nnATC
Cuts at: 3729 6487 3729
Size: 2758 3735
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2062 4300 4682 5317
Size: 53 83 186 936 529 2238 382 635
Cuts at: 5317 275
Size: 1451
Fragments arranged by size:
2238 1451 936 635 529 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2580 4926 5324 5598 5745 6156 2580
Size: 2346 398 274 147 411 2917
Fragments arranged by size:
2917 2346 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6268
Fragments arranged by size:
6268 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2857 1186
Size: 1671 4822
BseYI C'CCAG_C
Cuts at: 2097 2284 2816 5696 6192 2097
Size: 187 532 2880 496 2398
Fragments arranged by size:
2880 2398 532 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3536 1163
Size: 573 1800 4120
Fragments arranged by size:
4120 1800 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2107 2424 3943 4704 5732 1540
Size: 275 186 106 317 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 317 275 186 106
BsiHKAI G_wGCw'C
Cuts at: 2217 2382 3477 3693 4554 4639 5710 2217
Size: 165 1095 216 861 85 1071 3000
Fragments arranged by size:
3000 1095 1071 861 216 165 85
BsmI GAATG_Cn'
Cuts at: 2718 3151 4004 4097 2718
Size: 433 853 93 5114
Fragments arranged by size:
5114 853 433 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2217 2382 2439 2538 3206 3477
Size: 399 421 6 165 57 99 668 271
Cuts at: 3477 3693 3812 4554 4639 5710 1391
Size: 216 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 421 399 271 216
165 119 99 85 57 6
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 4382 6112 4382
Size: 1730 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3848 844
Size: 3004 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2088 2137 2326 4380 1394
Size: 429 265 49 189 2054 3507
Fragments arranged by size:
3507 2054 429 265 189 49
BsrDI GCAATG_nn'
Cuts at: 65 1248 2323 4988 5162 65
Size: 1183 1075 2665 174 1396
Fragments arranged by size:
2665 1396 1183 1075 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2308 2423 2580 5137 5324 1700
Size: 110 498 115 157 2557 187 2869
Fragments arranged by size:
2869 2557 498 187 157 115 110
BsrGI T'GTAC_A
Cuts at: 96 2830 96
Size: 2734 3759
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 2375 4553 5565 2375
Size: 2178 1012 3303
Fragments arranged by size:
3303 2178 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6493
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 2797 1184
Size: 1613 4880
BstYI r'GATC_y
Cuts at: 1972 3823 4190 4575 4592 6044 6130 6142 6488
Size: 1851 367 385 17 1452 86 12 346
Cuts at: 6488 1972
Size: 1977
Fragments arranged by size:
1977 1851 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2107 2529 513
Size: 552 939 103 422 4477
Fragments arranged by size:
4477 939 552 422 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5754
Fragments arranged by size:
5754 683 56
BtsI GCAGTG_nn'
Cuts at: 2501 2807 2988 3007 4009 4803 4823 2501
Size: 306 181 19 1002 794 20 4171
Fragments arranged by size:
4171 1002 794 306 181 20 19
ClaI AT'CG_AT
Cuts at: 1554 2127 1554
Size: 573 5920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3797 3797
Size: 6493
DraIII CAC_nnn'GTG
Cuts at: 3547 3547
Size: 6493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2104 2421 3940
Size: 54 1425 50 275 18 274 317 1519
Cuts at: 3940 4829 6458 8
Size: 889 1629 43
Fragments arranged by size:
1629 1519 1425 889 317 275 274 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2104 2421 3940 1537
Size: 275 292 317 1519 4090
Fragments arranged by size:
4090 1519 317 292 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 2222 3001 4423 1353
Size: 791 78 779 1422 3423
Fragments arranged by size:
3423 1422 791 779 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2237 2342 2476 5080 5454 5600
Size: 1064 138 648 105 134 2604 374 146
Cuts at: 5600 387
Size: 1280
Fragments arranged by size:
2604 1280 1064 648 374 146 138 134
105
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 6493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2762 3311 3486 3488 3657 4556
Size: 393 818 312 549 175 2 169 899
Cuts at: 4556 5153 5940 6208 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 899 818 787 597 549 393 312
268 175 169 2
EcoNI CCTnn'n_nnAGG
Cuts at: 2658 2658
Size: 6493
EcoO109I rG'GnC_Cy
Cuts at: 2534 2535 2846 3632 2534
Size: 1 311 786 5395
Fragments arranged by size:
5395 786 311 1
EcoRI G'AATT_C
Cuts at: 3802 4180 3802
Size: 378 6115
FseI GG_CCGG'CC
Cuts at: 2427 2427
Size: 6493
FspI TGC'GCA
Cuts at: 4999 4999
Size: 6493
HaeII r_GCGC'y
Cuts at: 1678 2015 2065 2392 2403 3766 5640 1678
Size: 337 50 327 11 1363 1874 2531
Fragments arranged by size:
2531 1874 1363 337 327 50 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 3308 3340 5134
Size: 32 135 32 1016 32 688 32 1794
Cuts at: 5134 5166 5208 5240 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1794 1016 688 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3837 4083 669
Size: 988 281 1899 246 3079
Fragments arranged by size:
3079 1899 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6493
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KasI G'GCGC_C
Cuts at: 2061 2061
Size: 6493
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 3070 4092 3070
Size: 1022 5471
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2813 2948 3316 5607 5791 1305
Size: 1508 135 368 2291 184 2007
Fragments arranged by size:
2291 2007 1508 368 184 135
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4671
Fragments arranged by size:
4671 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2961 4451 4810 4969 518
Size: 648 1795 1490 359 159 2042
Fragments arranged by size:
2042 1795 1490 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2109 2371 2464 2531 2930 3289 3909
Size: 491 12 262 93 67 399 359 620
Cuts at: 3909 4586 5734 5979 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 620 491 399 359 262
245 93 67 12
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 6493
NarI GG'CG_CC
Cuts at: 2062 2062
Size: 6493
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5002
Fragments arranged by size:
5002 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6493
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 6493
NheI G'CTAG_C
Cuts at: 1052 3281 3638 1052
Size: 2229 357 3907
Fragments arranged by size:
3907 2229 357
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NsiI A_TGCA'T
Cuts at: 2718 2718
Size: 6493
NspI r_CATG'y
Cuts at: 1132 1229 2837 3851 5396 1132
Size: 97 1608 1014 1545 2229
Fragments arranged by size:
2229 1608 1545 1014 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6493
PciI A'CATG_T
Cuts at: 2833 5392 2833
Size: 2559 3934
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2058 2097 1108
Size: 950 39 5504
Fragments arranged by size:
5504 950 39
PfoI T'CCnGG_A
Cuts at: 2719 2719
Size: 6493
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PmlI CAC'GTG
Cuts at: 3581 3581
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 6075 6107 4562
Size: 32 1481 32 4948
Fragments arranged by size:
4948 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2846 2846
Size: 6493
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PspOMI G'GGCC_C
Cuts at: 2534 2534
Size: 6493
PstI C_TGCA'G
Cuts at: 830 2508 2814 3083 3442 3845 830
Size: 1678 306 269 359 403 3478
Fragments arranged by size:
3478 1678 403 359 306 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2371 2930 3289 2371
Size: 559 359 5575
Fragments arranged by size:
5575 559 359
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 6493
SacII CC_GC'GG
Cuts at: 2110 2532 2110
Size: 422 6071
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SapI GCTCTTCn'nnn_
Cuts at: 2144 2222 2144
Size: 78 6415
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 2647 2906 3085 4741 6453 2647
Size: 259 179 1656 1712 2687
Fragments arranged by size:
2687 1712 1656 259 179
SexAI A'CCwGG_T
Cuts at: 3543 3543
Size: 6493
SfcI C'TryA_G
Cuts at: 826 1045 2504 2565 2810 2936 3079 3306 3438
Size: 219 1459 61 245 126 143 227 132
Cuts at: 3438 3841 4976 5657 5848 826
Size: 403 1135 681 191 1471
Fragments arranged by size:
1471 1459 1135 681 403 245 227 219
191 143 132 126 61
SfoI GGC'GCC
Cuts at: 2063 2063
Size: 6493
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 2167 3820 1872
Size: 295 1653 4545
Fragments arranged by size:
4545 1653 295
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 4597 5498 5760 820
Size: 197 926 441 2213 901 262 1553
Fragments arranged by size:
2213 1553 926 901 441 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 3851 1132
Size: 2719 3774
SrfI GCCC'GGGC
Cuts at: 2167 2167
Size: 6493
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6493
StyI C'CwwG_G
Cuts at: 513 1065 2004 2707 3932 513
Size: 552 939 703 1225 3074
Fragments arranged by size:
3074 1225 939 703 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4520 4537 4690 4875 5320 325
Size: 1255 2940 17 153 185 445 1498
Fragments arranged by size:
2940 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2645 2773 2830
Size: 274 80 33 51 1443 668 128 57
Cuts at: 2830 2904 3083 3381 4739 6451 96
Size: 74 179 298 1358 1712 138
Fragments arranged by size:
1712 1443 1358 668 298 274 179 138
128 80 74 57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6453 6485 6453
Size: 32 6461
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6493
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 2165 3818 1870
Size: 295 1653 4545
Fragments arranged by size:
4545 1653 295
XmnI GAAnn'nnTTC
Cuts at: 4622 4622
Size: 6493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoRV FalI FspAI NruI PacI
PspXI PsrI RsrII SanDI SfiI SwaI XcmI