Restriction map for
pFN21AB7815
(Circular) (Six-base) MAPSORT of: pFN21AB7815.seq Check: 1242 from: 1 to: 6301
pFN21AB7815 6301 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3644 1937
Size: 1707 4594
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4364 5748 6016 1632
Size: 2732 1384 268 1917
Fragments arranged by size:
2732 1917 1384 268
AfeI AGC'GCT
Cuts at: 2273 2273
Size: 6301
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2996 5200 6177 1203
Size: 1793 2204 977 1327
Fragments arranged by size:
2204 1793 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 3345 1166
Size: 2179 4122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AlwNI CAG_nnn'CTG
Cuts at: 2365 4044 5616 2365
Size: 1679 1572 3050
Fragments arranged by size:
3050 1679 1572
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2534 2934 3102 3610 3836 3988 6097 1493
Size: 1041 400 168 508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1041 508 400 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3626 1870
Size: 73 1683 4545
Fragments arranged by size:
4545 1683 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3622 5077 618
Size: 325 217 627 1835 1455 1842
Fragments arranged by size:
1842 1835 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3620 1391
Size: 2229 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2999 928
Size: 971 1100 4230
Fragments arranged by size:
4230 1100 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 2257 2671 3564 1548
Size: 709 414 893 4285
Fragments arranged by size:
4285 893 709 414
BfrBI ATG'CAT
Cuts at: 2327 2686 2327
Size: 359 5942
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 4362 5518 1790
Size: 2572 1156 2573
Fragments arranged by size:
2573 2572 1156
BmgBI CAC'GTC
Cuts at: 1318 2997 1318
Size: 1679 4622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3593 4032 4990
Size: 416 53 83 399 24 2176 439 958
Cuts at: 4990 442
Size: 1753
Fragments arranged by size:
2176 1753 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3385 3385
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2288 2719 4961 1239
Size: 815 234 431 2242 2579
Fragments arranged by size:
2579 2242 815 431 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2804 4426 5291 5589 2804
Size: 1622 865 298 3516
Fragments arranged by size:
3516 1622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3089 3196 4964 882
Size: 617 1590 107 1768 2219
Fragments arranged by size:
2219 1768 1590 617 107
BsaAI yAC'GTr
Cuts at: 493 1204 2399 4092 493
Size: 711 1195 1693 2702
Fragments arranged by size:
2702 1693 1195 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3035 4108 4490 5125
Size: 53 83 186 936 1502 1073 382 635
Cuts at: 5125 275
Size: 1451
Fragments arranged by size:
1502 1451 1073 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2164 2858 4734 5132 5406 5553 5964 2164
Size: 694 1876 398 274 147 411 2501
Fragments arranged by size:
2501 1876 694 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6076
Fragments arranged by size:
6076 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 3428 1186
Size: 892 1350 4059
Fragments arranged by size:
4059 1350 892
BseYI C'CCAG_C
Cuts at: 5504 6000 5504
Size: 496 5805
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2647 3067 3507 1163
Size: 573 911 420 440 3957
Fragments arranged by size:
3957 911 573 440 420
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4362 4447 5518 4362
Size: 85 1071 5145
Fragments arranged by size:
5145 1071 85
BsmI GAATG_Cn'
Cuts at: 2798 3541 3812 3905 2798
Size: 743 271 93 5194
Fragments arranged by size:
5194 743 271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3620 4362 4447 5518 1391
Size: 399 1830 742 85 1071 2174
Fragments arranged by size:
2174 1830 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 2314 2380 4190 5920 2314
Size: 66 1810 1730 2695
Fragments arranged by size:
2695 1810 1730 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3656 844
Size: 2812 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2344 2971 4796 4970 65
Size: 1183 1096 627 1825 174 1396
Fragments arranged by size:
1825 1396 1183 1096 627 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2923 96
Size: 2827 3474
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2032
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 3631 3998 4383 4400 5852 5938 5950 6296
Size: 1659 367 385 17 1452 86 12 346
Cuts at: 6296 1972
Size: 1977
Fragments arranged by size:
1977 1659 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3493 3493
Size: 6301
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2635 2887 3055 3546 3817 4611 4631 2148
Size: 487 252 168 491 271 794 20 3818
Fragments arranged by size:
3818 794 491 487 271 252 168 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2953 2988 529
Size: 35 2389 35 3842
Fragments arranged by size:
3842 2389 35 35
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3748 4637 6266
Size: 54 1425 50 275 18 1918 889 1629
Cuts at: 6266 8
Size: 43
Fragments arranged by size:
1918 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2180 4231 1353
Size: 827 2051 3423
Fragments arranged by size:
3423 2051 827
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2288 2719 4364 4961 5748 6016
Size: 393 422 234 431 1645 597 787 268
Cuts at: 6016 1239
Size: 1524
Fragments arranged by size:
1645 1524 787 597 431 422 393 268
234
EcoO109I rG'GnC_Cy
Cuts at: 2095 2095
Size: 6301
EcoRI G'AATT_C
Cuts at: 2934 3102 3610 3988 2934
Size: 168 508 378 5247
Fragments arranged by size:
5247 508 378 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 6301
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2085 2275 5448 1678
Size: 407 190 3173 2531
Fragments arranged by size:
3173 2531 407 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4942 4974 5016 5048 1373
Size: 32 135 32 3370 32 42 32 2626
Fragments arranged by size:
3370 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 3645 3891 669
Size: 988 281 496 1211 246 3079
Fragments arranged by size:
3079 1211 988 496 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 2434 3891 2434
Size: 1457 4844
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 2208 3900 2208
Size: 1692 4609
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 2163 5415 5599 1305
Size: 792 66 3252 184 2007
Fragments arranged by size:
3252 2007 792 184 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4479
Fragments arranged by size:
4479 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2319 2943 3345 4259 4618 4777 518
Size: 648 1153 624 402 914 359 159 2042
Fragments arranged by size:
2042 1153 914 648 624 402 359 159
MspA1I CmG'CkG
Cuts at: 1606 3432 3717 4394 5542 5787 1606
Size: 1826 285 677 1148 245 2120
Fragments arranged by size:
2120 1826 1148 677 285 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5749
NdeI CA'TA_TG
Cuts at: 387 2246 387
Size: 1859 4442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NsiI A_TGCA'T
Cuts at: 2329 2688 2329
Size: 359 5942
NspI r_CATG'y
Cuts at: 1132 1229 3659 5204 1132
Size: 97 2430 1545 2229
Fragments arranged by size:
2430 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2095
Size: 6301
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PstI C_TGCA'G
Cuts at: 830 3322 3653 830
Size: 2492 331 3478
Fragments arranged by size:
3478 2492 331
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SexAI A'CCwGG_T
Cuts at: 2133 2676 2133
Size: 543 5758
SfcI C'TryA_G
Cuts at: 826 1045 2125 3318 3649 4784 5465 5656 826
Size: 219 1080 1193 331 1135 681 191 1471
Fragments arranged by size:
1471 1193 1135 1080 681 331 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 4405 5306 5568 820
Size: 197 926 876 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 876 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 2338 4225 5
Size: 47 2286 1887 2081
Fragments arranged by size:
2286 2081 1887 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 3300 3740 513
Size: 552 2235 440 3074
Fragments arranged by size:
3074 2235 552 440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4328 4345 4498 4683 5128 325
Size: 1255 2748 17 153 185 445 1498
Fragments arranged by size:
2748 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2923 4547 6259
Size: 274 80 33 51 1443 946 1624 1712
Cuts at: 6259 96
Size: 138
Fragments arranged by size:
1712 1624 1443 946 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3459 3491 6261 6293 3459
Size: 32 2770 32 3467
Fragments arranged by size:
3467 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 2517 2645 2729 4430 2517
Size: 128 84 1701 4388
Fragments arranged by size:
4388 1701 128 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI