Restriction map for
pFN21AB7813
(Circular) (Six-base) MAPSORT of: pFN21AB7813.seq Check: 8278 from: 1 to: 6301
pFN21AB7813 6301 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2370 4111 278
Size: 53 83 186 936 834 1741 2468
Fragments arranged by size:
2468 1741 936 834 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3196 3644 1937
Size: 1259 448 4594
Fragments arranged by size:
4594 1259 448
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3078 3404 4364 5748 6016 1632
Size: 1446 326 960 1384 268 1917
Fragments arranged by size:
1917 1446 1384 960 326 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2054 5200 6177 1203
Size: 851 3146 977 1327
Fragments arranged by size:
3146 1327 977 851
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 2813 3274 5030 2813
Size: 461 1756 4084
Fragments arranged by size:
4084 1756 461
AleI CACnn'nnGTG
Cuts at: 1166 2427 1166
Size: 1261 5040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2424 2456 2424
Size: 32 6269
AlwNI CAG_nnn'CTG
Cuts at: 4044 5616 4044
Size: 1572 4729
ApaI G_GGCC'C
Cuts at: 2394 2394
Size: 6301
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 2164 3610 3836 3988 6097 1493
Size: 671 1446 226 152 2109 1697
Fragments arranged by size:
2109 1697 1446 671 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3626 1870
Size: 73 1683 4545
Fragments arranged by size:
4545 1683 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2794 3622 5077 618
Size: 325 217 627 1007 828 1455 1842
Fragments arranged by size:
1842 1455 1007 828 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2394 3380 3620 1391
Size: 1003 986 240 4072
Fragments arranged by size:
4072 1003 986 240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3394 928
Size: 971 1495 3835
Fragments arranged by size:
3835 1495 971
BbvCI CC'TCA_GC
Cuts at: 3012 3012
Size: 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4492 4526 1903
Size: 34 50 34 2471 34 3678
Fragments arranged by size:
3678 2471 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3349 4193 5403 3349
Size: 844 1210 4247
Fragments arranged by size:
4247 1210 844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6301
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 2949 3079 3718 2949
Size: 130 639 5532
Fragments arranged by size:
5532 639 130
Bme1580I G_kGCm'C
Cuts at: 1790 2394 4362 5518 1790
Size: 604 1968 1156 2573
Fragments arranged by size:
2573 1968 1156 604
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3062 4032 4990
Size: 416 53 83 399 24 1645 970 958
Cuts at: 4990 442
Size: 1753
Fragments arranged by size:
1753 1645 970 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2532 2672 2675 4961 1239
Size: 1293 140 3 2286 2579
Fragments arranged by size:
2579 2286 1293 140 3
Bpu10I CC'TnA_GC
Cuts at: 2216 3012 2216
Size: 796 5505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2098 4426 5291 5589 2098
Size: 2328 865 298 2810
Fragments arranged by size:
2810 2328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2800 4964 882
Size: 617 1301 2164 2219
Fragments arranged by size:
2219 2164 1301 617
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 2565 4108 4490
Size: 53 83 186 936 834 198 1543 382
Cuts at: 4490 5125 275
Size: 635 1451
Fragments arranged by size:
1543 1451 936 834 635 382 198 186
83 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3352 3382 1178
Size: 30 165 30 1949 30 4097
Fragments arranged by size:
4097 1949 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2672 1186
Size: 1486 4815
BseYI C'CCAG_C
Cuts at: 2711 3221 3322 5504 6000 2711
Size: 510 101 2182 496 3012
Fragments arranged by size:
3012 2182 510 496 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5728
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 3447 3591 4362 4447 5518 2664
Size: 783 144 771 85 1071 3447
Fragments arranged by size:
3447 1071 783 771 144 85
BsmI GAATG_Cn'
Cuts at: 3527 3812 3905 3527
Size: 285 93 5923
Fragments arranged by size:
5923 285 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2664 3380 3447 3591 3620 4362
Size: 399 604 270 716 67 144 29 742
Cuts at: 4362 4447 5518 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 604 399 270 144
85 67 29
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 2721 3148 4190 5920 2721
Size: 427 1042 1730 3102
Fragments arranged by size:
3102 1730 1042 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2707 2818 3656 844
Size: 1863 111 838 3489
Fragments arranged by size:
3489 1863 838 111
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2008 4796 4970 65
Size: 1183 760 2788 174 1396
Fragments arranged by size:
2788 1396 1183 760 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3477 96
Size: 3381 2920
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 4361 5373 4361
Size: 1012 5289
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2382 3231 794
Size: 1588 849 3864
Fragments arranged by size:
3864 1588 849
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 3490 3490
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 3631 3998 4383 4400 5852 5938 5950 6296
Size: 1659 367 385 17 1452 86 12 346
Cuts at: 6296 1972
Size: 1977
Fragments arranged by size:
1977 1659 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2582 3372 2582
Size: 790 5511
BtgI C'CryG_G
Cuts at: 513 1065 3499 513
Size: 552 2434 3315
Fragments arranged by size:
3315 2434 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 3445 3817 4611 4631 3445
Size: 372 794 20 5115
Fragments arranged by size:
5115 794 372 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2605 2640 3514 3549 529
Size: 35 2041 35 874 35 3281
Fragments arranged by size:
3281 2041 874 35 35 35
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3192 5308 809
Size: 507 1876 2116 1802
Fragments arranged by size:
2116 1876 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3748 4637 6266
Size: 54 1425 50 275 18 1918 889 1629
Cuts at: 6266 8
Size: 43
Fragments arranged by size:
1918 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 4231 1353
Size: 837 2041 3423
Fragments arranged by size:
3423 2041 837
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2672 2675 3078 3404 4364 4961
Size: 393 900 140 3 403 326 960 597
Cuts at: 4961 5748 6016 1239
Size: 787 268 1524
Fragments arranged by size:
1524 960 900 787 597 403 393 326
268 140 3
EcoO109I rG'GnC_Cy
Cuts at: 2586 3016 3429 3511 2586
Size: 430 413 82 5376
Fragments arranged by size:
5376 430 413 82
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3099 3342 3099
Size: 243 6058
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 5448 1678
Size: 3770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2799 2831 2947
Size: 32 135 32 524 32 671 32 116
Cuts at: 2947 2979 3260 3292 3551 3583 4942 4974 5016
Size: 32 281 32 259 32 1359 32 42
Cuts at: 5016 5048 1373
Size: 32 2626
Fragments arranged by size:
2626 1359 671 524 281 259 135 116
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3197 3645 3891 669
Size: 988 281 1259 448 246 3079
Fragments arranged by size:
3079 1259 988 448 281 246
HindIII A'AGCT_T
Cuts at: 748 3348 748
Size: 2600 3701
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 2100 3900 2100
Size: 1800 4501
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5415 5599 1305
Size: 4110 184 2007
Fragments arranged by size:
4110 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4479
Fragments arranged by size:
4479 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2427 3488 3520 4259 4618 4777 518
Size: 648 1261 1061 32 739 359 159 2042
Fragments arranged by size:
2042 1261 1061 739 648 359 159 32
MspA1I CmG'CkG
Cuts at: 1606 3717 4394 5542 5787 1606
Size: 2111 677 1148 245 2120
Fragments arranged by size:
2120 2111 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3499 513
Size: 552 2434 3315
Fragments arranged by size:
3315 2434 552
NdeI CA'TA_TG
Cuts at: 387 2935 2967 387
Size: 2548 32 3721
Fragments arranged by size:
3721 2548 32
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2138 3659 5204 1132
Size: 97 909 1521 1545 2229
Fragments arranged by size:
2229 1545 1521 909 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2596 1108
Size: 1488 4813
PfoI T'CCnGG_A
Cuts at: 2310 2310
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 4370 4402 5883 5915 2507
Size: 32 1831 32 1481 32 2893
Fragments arranged by size:
2893 1831 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2586 3511 2586
Size: 925 5376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3074 3871 3074
Size: 797 5504
PspOMI G'GGCC_C
Cuts at: 2390 2390
Size: 6301
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3195 3643 1936
Size: 1259 448 4594
Fragments arranged by size:
4594 1259 448
SanDI GG'GwC_CC
Cuts at: 3511 3511
Size: 6301
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 2539 2551 3538 3649 4784 5465 5656
Size: 219 1494 12 987 111 1135 681 191
Cuts at: 5656 826
Size: 1471
Fragments arranged by size:
1494 1471 1135 987 681 219 191 111
12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2113 4405 5306 5568 820
Size: 197 926 170 2292 901 262 1553
Fragments arranged by size:
2292 1553 926 901 262 197 170
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 2138 3659 1132
Size: 1006 1521 3774
Fragments arranged by size:
3774 1521 1006
SspI AAT'ATT
Cuts at: 5 52 2785 4225 5
Size: 47 2733 1440 2081
Fragments arranged by size:
2733 2081 1440 47
StuI AGG'CCT
Cuts at: 1517 3215 1517
Size: 1698 4603
StyI C'CwwG_G
Cuts at: 513 1065 3004 3283 3363 3499 3740 513
Size: 552 1939 279 80 136 241 3074
Fragments arranged by size:
3074 1939 552 279 241 136 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 4328 4345 4498 4683 5128 325
Size: 1255 557 2191 17 153 185 445 1498
Fragments arranged by size:
2191 1498 1255 557 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3477 4547 6259
Size: 274 80 33 51 1443 1500 1070 1712
Cuts at: 6259 96
Size: 138
Fragments arranged by size:
1712 1500 1443 1070 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6261 6293 6261
Size: 32 6269
Tth111I GACn'n_nGTC
Cuts at: 1316 3470 1316
Size: 2154 4147
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2401
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 4430 2522
Size: 1908 4393
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2368 4109 276
Size: 53 83 186 936 834 1741 2468
Fragments arranged by size:
2468 1741 936 834 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI