Restriction map for
pFN21AB7810
(Circular) (Six-base) MAPSORT of: pFN21AB7810.seq Check: 9083 from: 1 to: 6490
pFN21AB7810 6490 bp
With 182 enzymes: SgfI *
April 15, 2010 09:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2374 2374
Size: 6490
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2220 3833 1937
Size: 283 1613 4594
Fragments arranged by size:
4594 1613 283
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2565 2998 3422 4553 5937 6205 1632
Size: 933 433 424 1131 1384 268 1917
Fragments arranged by size:
1917 1384 1131 933 433 424 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 5389 6366 1203
Size: 4186 977 1327
Fragments arranged by size:
4186 1327 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AlwNI CAG_nnn'CTG
Cuts at: 3514 4233 5805 3514
Size: 719 1572 4199
Fragments arranged by size:
4199 1572 719
ApaLI G'TGCA_C
Cuts at: 2683 4547 5703 2683
Size: 1864 1156 3470
Fragments arranged by size:
3470 1864 1156
ApoI r'AATT_y
Cuts at: 1493 2461 3799 4025 4177 6286 1493
Size: 968 1338 226 152 2109 1697
Fragments arranged by size:
2109 1697 1338 968 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 2249 3815 1870
Size: 73 225 81 1566 4545
Fragments arranged by size:
4545 1566 225 81 73
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2386 3094 3604 3811
Size: 325 217 627 387 212 708 510 207
Cuts at: 3811 5266 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 708 627 510 387 325 217
212 207
BanII G_rGCy'C
Cuts at: 1391 2079 2324 2412 2976 3722 3809 1391
Size: 688 245 88 564 746 87 4072
Fragments arranged by size:
4072 746 688 564 245 88 87
BbeI G_GCGC'C
Cuts at: 2178 2178
Size: 6490
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3076 3110 4681 4715 1903
Size: 34 1139 34 1571 34 3678
Fragments arranged by size:
3678 1571 1139 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2760 4382 5592 2760
Size: 1622 1210 3658
Fragments arranged by size:
3658 1622 1210
BclI T'GATC_A
Cuts at: 1548 2895 3075 3124 1548
Size: 1347 180 49 4914
Fragments arranged by size:
4914 1347 180 49
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2484 5101 136
Size: 107 122 71 631 605 812 2617 1525
Fragments arranged by size:
2617 1525 812 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3273 6485 3273
Size: 3212 3278
BlpI GC'TnA_GC
Cuts at: 2970 3907 2970
Size: 937 5553
Bme1580I G_kGCm'C
Cuts at: 1790 2391 2687 3609 4551 5707 1790
Size: 601 296 922 942 1156 2573
Fragments arranged by size:
2573 1156 942 922 601 296
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3091 4221 5179
Size: 416 53 83 399 24 1674 1130 958
Cuts at: 5179 442
Size: 1753
Fragments arranged by size:
1753 1674 1130 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2129 2420 2874 3393 3647 5150 1239
Size: 854 36 291 454 519 254 1503 2579
Fragments arranged by size:
2579 1503 854 519 454 291 254 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 4615 5480 5778 2081
Size: 2534 865 298 2793
Fragments arranged by size:
2793 2534 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3179 5153 882
Size: 617 1680 1974 2219
Fragments arranged by size:
2219 1974 1680 617
BsaAI yAC'GTr
Cuts at: 493 1204 4281 493
Size: 711 3077 2702
Fragments arranged by size:
3077 2702 711
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 2476 3639 4297
Size: 53 83 186 936 642 301 1163 658
Cuts at: 4297 4679 5314 275
Size: 382 635 1451
Fragments arranged by size:
1451 1163 936 658 642 635 382 301
186 83 53
BsaWI w'CCGG_w
Cuts at: 4923 5321 5595 5742 6153 4923
Size: 398 274 147 411 5260
Fragments arranged by size:
5260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3326 3356 3563 3593 1178
Size: 30 165 30 1923 30 207 30 4075
Fragments arranged by size:
4075 1923 207 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2799 3347 3515 3518 3701 1186
Size: 1613 548 168 3 183 3975
Fragments arranged by size:
3975 1613 548 183 168 3
BseYI C'CCAG_C
Cuts at: 2411 3519 5693 6189 2411
Size: 1108 2174 496 2712
Fragments arranged by size:
2712 2174 1108 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2580 1163
Size: 573 585 259 5073
Fragments arranged by size:
5073 585 573 259
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2202 2940 3789 3940 4701 5729
Size: 275 186 201 738 849 151 761 1028
Cuts at: 5729 1540
Size: 2301
Fragments arranged by size:
2301 1028 849 761 738 275 201 186
151
BsiHKAI G_wGCw'C
Cuts at: 2687 3198 4551 4636 5707 2687
Size: 511 1353 85 1071 3470
Fragments arranged by size:
3470 1353 1071 511 85
BsmI GAATG_Cn'
Cuts at: 2447 3733 4001 4094 2447
Size: 1286 268 93 4843
Fragments arranged by size:
4843 1286 268 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3683 3683
Size: 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2324 2391 2412 2687 2976 3198
Size: 399 289 245 67 21 275 289 222
Cuts at: 3198 3609 3722 3809 4551 4636 5707 1391
Size: 411 113 87 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 289 289 275
245 222 113 87 85 67 21
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 2892 4379 6109 2892
Size: 1487 1730 3273
Fragments arranged by size:
3273 1730 1487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2206 2374 3627 3845 844
Size: 1362 168 1253 218 3489
Fragments arranged by size:
3489 1362 1253 218 168
BsrBI CCG'CTC
Cuts at: 1394 1823 2113 2890 4377 1394
Size: 429 290 777 1487 3507
Fragments arranged by size:
3507 1487 777 429 290
BsrDI GCAATG_nn'
Cuts at: 65 1248 2575 4985 5159 65
Size: 1183 1327 2410 174 1396
Fragments arranged by size:
2410 1396 1327 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2231 2435 2443 2724 5134 5321 1700
Size: 110 421 204 8 281 2410 187 2869
Fragments arranged by size:
2869 2410 421 281 204 187 110 8
BsrGI T'GTAC_A
Cuts at: 96 2157 3378 3579 96
Size: 2061 1221 201 3007
Fragments arranged by size:
3007 2061 1221 201
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6490
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3591 3739 794
Size: 2797 148 3545
Fragments arranged by size:
3545 2797 148
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2284
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 2055 2100 3159 3273 3820 4187 4572 4589
Size: 83 45 1059 114 547 367 385 17
Cuts at: 4589 6041 6127 6139 6485 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1059 547 385 367 346 114
86 83 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2196 2374 3189 3667 3681 3783 513
Size: 552 1131 178 815 478 14 102 3220
Fragments arranged by size:
3220 1131 815 552 478 178 102 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3714 487
Size: 683 56 2488 3263
Fragments arranged by size:
3263 2488 683 56
BtsI GCAGTG_nn'
Cuts at: 2568 3711 4006 4800 4820 2568
Size: 1143 295 794 20 4238
Fragments arranged by size:
4238 1143 794 295 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6455
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2948 5497 809
Size: 507 1632 2549 1802
Fragments arranged by size:
2549 1802 1632 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2377 2499
Size: 54 1425 50 275 18 369 178 122
Cuts at: 2499 2879 2937 3474 3708 3937 4826 6455 8
Size: 380 58 537 234 229 889 1629 43
Fragments arranged by size:
1629 1425 889 537 380 369 275 234
229 178 122 58 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2199 2937 3937 1537
Size: 275 387 738 1000 4090
Fragments arranged by size:
4090 1000 738 387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2702 2776 3083 3408 4420 1353
Size: 1349 74 307 325 1012 3423
Fragments arranged by size:
3423 1349 1012 325 307 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5077 5451 5597 387
Size: 1064 138 3488 374 146 1280
Fragments arranged by size:
3488 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2129 2420 2565 2874 2998 3393
Size: 393 461 36 291 145 309 124 395
Cuts at: 3393 3422 3647 4553 5150 5937 6205 1239
Size: 29 225 906 597 787 268 1524
Fragments arranged by size:
1524 906 787 597 461 395 393 309
291 268 225 145 124 36 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2123
Size: 6490
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 6490
FseI GG_CCGG'CC
Cuts at: 2439 2439
Size: 6490
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 2178 2812 3187 5637 1678
Size: 500 634 375 2450 2531
Fragments arranged by size:
2531 2450 634 500 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3157 3189 5131 5163 5205
Size: 32 135 32 1585 32 1942 32 42
Cuts at: 5205 5237 1373
Size: 32 2626
Fragments arranged by size:
2626 1942 1585 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3021 748
Size: 2273 4217
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KasI G'GCGC_C
Cuts at: 2174 2174
Size: 6490
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2301 2943 5604 5788 1305
Size: 996 642 2661 184 2007
Fragments arranged by size:
2661 2007 996 642 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2501 2881 3476 3710 10
Size: 54 1425 343 669 380 595 234 2790
Fragments arranged by size:
2790 1425 669 595 380 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4448 4807 4966 518
Size: 648 843 2439 359 159 2042
Fragments arranged by size:
2439 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2198 2326 2353 2415 3511 3906 4583 5731
Size: 592 128 27 62 1096 395 677 1148
Cuts at: 5731 5976 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1096 677 592 395 245 128
62 27
NaeI GCC'GGC
Cuts at: 2437 2445 2726 2437
Size: 8 281 6201
Fragments arranged by size:
6201 281 8
NarI GG'CG_CC
Cuts at: 2175 2175
Size: 6490
NcoI C'CATG_G
Cuts at: 513 1065 3189 513
Size: 552 2124 3814
Fragments arranged by size:
3814 2124 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NgoMIV G'CCGG_C
Cuts at: 2435 2443 2724 2435
Size: 8 281 6201
Fragments arranged by size:
6201 281 8
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 2199 3937 2199
Size: 1738 4752
NspI r_CATG'y
Cuts at: 1132 1229 3286 3656 3848 5393 1132
Size: 97 2057 370 192 1545 2229
Fragments arranged by size:
2229 2057 1545 370 192 97
PasI CC'CwG_GG
Cuts at: 1348 2119 3200 1348
Size: 771 1081 4638
Fragments arranged by size:
4638 1081 771
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2877 3472 3514 1108
Size: 1769 595 42 4084
Fragments arranged by size:
4084 1769 595 42
PfoI T'CCnGG_A
Cuts at: 2033 3438 2033
Size: 1405 5085
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 6072 6104 4559
Size: 32 1481 32 4945
Fragments arranged by size:
4945 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PstI C_TGCA'G
Cuts at: 830 2370 3327 3498 3842 830
Size: 1540 957 171 344 3478
Fragments arranged by size:
3478 1540 957 344 171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2353 2353
Size: 6490
SacII CC_GC'GG
Cuts at: 2199 2199
Size: 6490
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SapI GCTCTTCn'nnn_
Cuts at: 2702 2702
Size: 6490
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SfcI C'TryA_G
Cuts at: 826 1045 2366 2452 3323 3494 3838 4973 5654
Size: 219 1321 86 871 171 344 1135 681
Cuts at: 5654 5845 826
Size: 191 1471
Fragments arranged by size:
1471 1321 1135 871 681 344 219 191
171 86
SfoI GGC'GCC
Cuts at: 2176 2176
Size: 6490
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 2251 3817 1872
Size: 379 1566 4545
Fragments arranged by size:
4545 1566 379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 4594 5495 5757 820
Size: 197 926 153 2498 901 262 1553
Fragments arranged by size:
2498 1553 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 2624 2720 3486 1517
Size: 1107 96 766 4521
Fragments arranged by size:
4521 1107 766 96
StyI C'CwwG_G
Cuts at: 513 1065 2059 2541 3189 3477 3929 513
Size: 552 994 482 648 288 452 3074
Fragments arranged by size:
3074 994 648 552 482 452 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2793 3213 4517 4534 4687 4872 5317
Size: 1255 1213 420 1304 17 153 185 445
Cuts at: 5317 325
Size: 1498
Fragments arranged by size:
1498 1304 1255 1213 445 420 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 3378 3579
Size: 274 80 33 51 1443 180 1221 201
Cuts at: 3579 4736 6448 96
Size: 1157 1712 138
Fragments arranged by size:
1712 1443 1221 1157 274 201 180 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 6450 6482 3326
Size: 32 3092 32 3334
Fragments arranged by size:
3334 3092 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3139 3826 3139
Size: 687 5803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2819 2819
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 2249 3815 1870
Size: 379 1566 4545
Fragments arranged by size:
4545 1566 379
XmnI GAAnn'nnTTC
Cuts at: 4619 4619
Size: 6490
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FspAI NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI
SwaI