Restriction map for
pFN21AB7806
(Circular) (Six-base) MAPSORT of: pFN21AB7806.seq Check: 1002 from: 1 to: 6484
pFN21AB7806 6484 bp
With 182 enzymes: SgfI *
March 28, 2012 12:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 1937
Size: 1890 4594
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4547 5931 6199 1632
Size: 2915 1384 268 1917
Fragments arranged by size:
2915 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 5383 6360 1203
Size: 4180 977 1327
Fragments arranged by size:
4180 1327 977
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6484
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 6452
AlwNI CAG_nnn'CTG
Cuts at: 2065 2083 2495 3233 3290 4227 5799 2065
Size: 18 412 738 57 937 1572 2750
Fragments arranged by size:
2750 1572 937 738 412 57 18
ApaI G_GGCC'C
Cuts at: 2122 2259 2486 2122
Size: 137 227 6120
Fragments arranged by size:
6120 227 137
ApaLI G'TGCA_C
Cuts at: 3432 4541 5697 3432
Size: 1109 1156 4219
Fragments arranged by size:
4219 1156 1109
ApoI r'AATT_y
Cuts at: 1493 2732 3793 4019 4171 6280 1493
Size: 1239 1061 226 152 2109 1697
Fragments arranged by size:
2109 1697 1239 1061 226 152
AscI GG'CGCG_CC
Cuts at: 2054 2054
Size: 6484
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 2150 2286 2309 2411 3809 1870
Size: 73 178 29 136 23 102 1398 4545
Fragments arranged by size:
4545 1398 178 136 102 73 29 23
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2507 3054 3539 3805 5260
Size: 325 217 627 720 547 485 266 1455
Cuts at: 5260 618
Size: 1842
Fragments arranged by size:
1842 1455 720 627 547 485 325 266
217
BanII G_rGCy'C
Cuts at: 1391 2122 2151 2176 2259 2306 2486 3018 3803
Size: 731 29 25 83 47 180 532 785
Cuts at: 3803 1391
Size: 4072
Fragments arranged by size:
4072 785 731 532 180 83 47 29
25
BbeI G_GCGC'C
Cuts at: 3058 3058
Size: 6484
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2412 928
Size: 971 513 5000
Fragments arranged by size:
5000 971 513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2081 2115 4675 4709 1903
Size: 34 144 34 2560 34 3678
Fragments arranged by size:
3678 2560 144 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 2201 2241
Size: 107 122 71 631 605 517 12 40
Cuts at: 2241 2490 3401 3461 3739 5095 136
Size: 249 911 60 278 1356 1525
Fragments arranged by size:
1525 1356 911 631 605 517 278 249
122 107 71 60 40 12
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3612 3901 3612
Size: 289 6195
Bme1580I G_kGCm'C
Cuts at: 1790 2122 2161 2259 2486 2510 3103 3436 3542
Size: 332 39 98 227 24 593 333 106
Cuts at: 3542 4545 5701 1790
Size: 1003 1156 2573
Fragments arranged by size:
2573 1156 1003 593 333 332 227 106
98 39 24
BmgBI CAC'GTC
Cuts at: 1318 2919 1318
Size: 1601 4883
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4215 5173 442
Size: 416 53 83 399 24 2798 958 1753
Fragments arranged by size:
2798 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 3002 3383 3422 5144 1239
Size: 848 915 381 39 1722 2579
Fragments arranged by size:
2579 1722 915 848 381 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4609 5474 5772 4609
Size: 865 298 5321
Fragments arranged by size:
5321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5147 882
Size: 617 3648 2219
Fragments arranged by size:
3648 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 2833 4275 493
Size: 711 1431 198 1442 2702
Fragments arranged by size:
2702 1442 1431 711 198
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3055 4291 4673 5308
Size: 53 83 186 936 1522 1236 382 635
Cuts at: 5308 275
Size: 1451
Fragments arranged by size:
1522 1451 1236 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3379 3707 4917 5315 5589 5736 6147 3379
Size: 328 1210 398 274 147 411 3716
Fragments arranged by size:
3716 1210 411 398 328 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 2342 2372 3155
Size: 30 165 30 615 30 294 30 783
Cuts at: 3155 3185 3420 3450 3625 3655 1178
Size: 30 235 30 175 30 4007
Fragments arranged by size:
4007 783 615 294 235 175 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2288 2351 2402 2405 2408 2438 2441
Size: 1099 3 63 51 3 3 30 3
Cuts at: 2441 2444 2447 2450 2468 2471 2474 2477 2486
Size: 3 3 3 18 3 3 3 9
Cuts at: 2486 2696 3106 3218 1186
Size: 210 410 112 4452
Fragments arranged by size:
4452 1099 410 210 112 63 51 30
18 9 3 3 3 3 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2227 2479 2535 2578 3465 3728 5687 6183 2227
Size: 252 56 43 887 263 1959 496 2528
Fragments arranged by size:
2528 1959 887 496 263 252 56 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2088 2952 3309 1163
Size: 573 352 864 357 4338
Fragments arranged by size:
4338 864 573 357 352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 2597 3694 3934 4695 5723
Size: 275 186 115 481 1097 240 761 1028
Cuts at: 5723 1540
Size: 2301
Fragments arranged by size:
2301 1097 1028 761 481 275 240 186
115
BsiHKAI G_wGCw'C
Cuts at: 2343 3018 3436 4545 4630 5701 2343
Size: 675 418 1109 85 1071 3126
Fragments arranged by size:
3126 1109 1071 675 418 85
BsmI GAATG_Cn'
Cuts at: 3480 3995 4088 3480
Size: 515 93 5876
Fragments arranged by size:
5876 515 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2021 2021
Size: 6484
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2151 2161 2176 2259 2306 2343
Size: 399 332 29 10 15 83 47 37
Cuts at: 2343 2486 2510 3018 3103 3436 3542 3803 4545
Size: 143 24 508 85 333 106 261 742
Cuts at: 4545 4630 5701 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 333 332 261
143 106 85 85 83 47 37 29
24 15 10
BspEI T'CCGG_A
Cuts at: 3379 6147 3379
Size: 2768 3716
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2919 3839 844
Size: 2075 920 3489
Fragments arranged by size:
3489 2075 920
BsrBI CCG'CTC
Cuts at: 1394 1823 2593 4371 1394
Size: 429 770 1778 3507
Fragments arranged by size:
3507 1778 770 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4979 5153 65
Size: 1183 3731 174 1396
Fragments arranged by size:
3731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3051 3619 5128 5315 1700
Size: 110 1241 568 1509 187 2869
Fragments arranged by size:
2869 1509 1241 568 187 110
BsrGI T'GTAC_A
Cuts at: 96 3591 96
Size: 3495 2989
BssHII G'CGCG_C
Cuts at: 1925 2054 1925
Size: 129 6355
BssSI C'ACGA_G
Cuts at: 2858 4544 5556 2858
Size: 1686 1012 3786
Fragments arranged by size:
3786 1686 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2083 794
Size: 1289 5195
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 3065 2578
Size: 487 5997
BstYI r'GATC_y
Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479
Size: 1842 367 385 17 1452 86 12 346
Cuts at: 6479 1972
Size: 1977
Fragments arranged by size:
1977 1842 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2511 2554 2662 3456 513
Size: 552 1446 43 108 794 3541
Fragments arranged by size:
3541 1446 794 552 108 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2574 487
Size: 683 56 1348 4397
Fragments arranged by size:
4397 1348 683 56
BtsI GCAGTG_nn'
Cuts at: 4000 4794 4814 4000
Size: 794 20 5670
Fragments arranged by size:
5670 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 3268 3268
Size: 6484
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2181 2594
Size: 54 1425 50 275 18 283 68 413
Cuts at: 2594 2799 3150 3235 3402 3453 3691 3721 3931
Size: 205 351 85 167 51 238 30 210
Cuts at: 3931 4820 6449 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 413 351 283 275 238
210 205 167 85 68 54 51 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 2594 3691 3931 1537
Size: 275 301 481 1097 240 4090
Fragments arranged by size:
4090 1097 481 301 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 4414 1353
Size: 3061 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5071 5445 5591 387
Size: 1064 138 3482 374 146 1280
Fragments arranged by size:
3482 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3016 3016
Size: 6484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 3002 3383 3422 4547 5144 5931
Size: 393 455 915 381 39 1125 597 787
Cuts at: 5931 6199 1239
Size: 268 1524
Fragments arranged by size:
1524 1125 915 787 597 455 393 381
268 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2414 2861 2414
Size: 447 6037
EcoO109I rG'GnC_Cy
Cuts at: 2256 2483 2492 2600 2716 2809 2902 2995 2256
Size: 227 9 108 116 93 93 93 5745
Fragments arranged by size:
5745 227 116 108 93 93 93 9
EcoRI G'AATT_C
Cuts at: 2732 3793 4171 2732
Size: 1061 378 5045
Fragments arranged by size:
5045 1061 378
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 3058 5631 1678
Size: 1380 2573 2531
Fragments arranged by size:
2573 2531 1380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 5125 5157 5199
Size: 32 135 32 770 32 2751 32 42
Cuts at: 5199 5231 1373
Size: 32 2626
Fragments arranged by size:
2751 2626 770 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3828 4074 669
Size: 988 281 1890 246 3079
Fragments arranged by size:
3079 1890 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KasI G'GCGC_C
Cuts at: 3054 3054
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 5598 5782 1305
Size: 1080 3213 184 2007
Fragments arranged by size:
3213 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2801 3455 10
Size: 54 1425 343 969 654 3039
Fragments arranged by size:
3039 1425 969 654 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3563 3569 4442 4801 4960 518
Size: 648 2397 6 873 359 159 2042
Fragments arranged by size:
2397 2042 873 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2041 2050 2083 2170 2210 2556 2647 3290
Size: 435 9 33 87 40 346 91 643
Cuts at: 3290 3317 3335 3349 3469 3717 3737 3900 4577
Size: 27 18 14 120 248 20 163 677
Cuts at: 4577 5725 5970 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 643 435 346 248 245
163 120 91 87 40 33 27 20
18 14 9
NaeI GCC'GGC
Cuts at: 3053 3621 3053
Size: 568 5916
NarI GG'CG_CC
Cuts at: 3055 3055
Size: 6484
NcoI C'CATG_G
Cuts at: 513 1065 3456 513
Size: 552 2391 3541
Fragments arranged by size:
3541 2391 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6484
NgoMIV G'CCGG_C
Cuts at: 3051 3619 3051
Size: 568 5916
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 3112 3568 3842 5387 1132
Size: 97 1883 456 274 1545 2229
Fragments arranged by size:
2229 1883 1545 456 274 97
PasI CC'CwG_GG
Cuts at: 1348 2571 3142 1348
Size: 1223 571 4690
Fragments arranged by size:
4690 1223 571
PciI A'CATG_T
Cuts at: 5383 5383
Size: 6484
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 3268 1108
Size: 957 1203 4324
Fragments arranged by size:
4324 1203 957
PfoI T'CCnGG_A
Cuts at: 2736 2736
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2716 2809 2716
Size: 93 6391
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2118 2255 2482 2118
Size: 137 227 6120
Fragments arranged by size:
6120 227 137
PstI C_TGCA'G
Cuts at: 830 2209 2652 3373 3836 830
Size: 1379 443 721 463 3478
Fragments arranged by size:
3478 1379 721 463 443
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2083 2210 2647 3290 3317 3335 3349 3469 2083
Size: 127 437 643 27 18 14 120 5098
Fragments arranged by size:
5098 643 437 127 120 27 18 14
SacI G_AGCT'C
Cuts at: 3018 3018
Size: 6484
SacII CC_GC'GG
Cuts at: 2557 2557
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SanDI GG'GwC_CC
Cuts at: 2716 2716
Size: 6484
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 2012 2205 2648 3369 3832 4967 5648
Size: 219 967 193 443 721 463 1135 681
Cuts at: 5648 5839 826
Size: 191 1471
Fragments arranged by size:
1471 1135 967 721 681 463 443 219
193 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2490 3401 3461 2490
Size: 911 60 5513
Fragments arranged by size:
5513 911 60
SfoI GGC'GCC
Cuts at: 3056 3056
Size: 6484
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 2123 2288 2311 3811 1872
Size: 251 165 23 1500 4545
Fragments arranged by size:
4545 1500 251 165 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4588 5489 5751 820
Size: 197 926 2645 901 262 1553
Fragments arranged by size:
2645 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3112 3842 1132
Size: 1980 730 3774
Fragments arranged by size:
3774 1980 730
SrfI GCCC'GGGC
Cuts at: 2123 2123
Size: 6484
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 2549 1517
Size: 1032 5452
StyI C'CwwG_G
Cuts at: 513 1065 2898 3456 3923 513
Size: 552 1833 558 467 3074
Fragments arranged by size:
3074 1833 558 552 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2241 3136 4511 4528 4681 4866 5311
Size: 1255 661 895 1375 17 153 185 445
Cuts at: 5311 325
Size: 1498
Fragments arranged by size:
1498 1375 1255 895 661 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3591 4730 6442
Size: 274 80 33 51 1443 1614 1139 1712
Cuts at: 6442 96
Size: 138
Fragments arranged by size:
1712 1614 1443 1139 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6444 6476 6444
Size: 32 6452
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3065 3065
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 2121 2286 2309 3809 1870
Size: 251 165 23 1500 4545
Fragments arranged by size:
4545 1500 251 165 23
XmnI GAAnn'nnTTC
Cuts at: 2732 4613 2732
Size: 1881 4603
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SapI SexAI SwaI