Restriction map for
pFN21AB7805
(Circular) (Six-base) MAPSORT of: pFN21AB7805.seq Check: 2217 from: 1 to: 6271
pFN21AB7805 6271 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2028 2028
Size: 6271
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 1937
Size: 1677 4594
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2328 3020 3082 3334 4334 5718 5986 1632
Size: 696 692 62 252 1000 1384 268 1917
Fragments arranged by size:
1917 1384 1000 696 692 268 252 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 3326 5170 6147 1203
Size: 2123 1844 977 1327
Fragments arranged by size:
2123 1844 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 4014 5586 4014
Size: 1572 4699
ApaI G_GGCC'C
Cuts at: 2116 2116
Size: 6271
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 2598 2628 2712 2796 3048 3132 3216 3300
Size: 1105 30 84 84 252 84 84 84
Cuts at: 3300 3384 3580 3806 3958 6067 1493
Size: 84 196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1105 252 226 196 152 84
84 84 84 84 84 30
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3596 1870
Size: 73 1653 4545
Fragments arranged by size:
4545 1653 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2768 2801 2768
Size: 33 6238
BamHI G'GATC_C
Cuts at: 3601 3968 3601
Size: 367 5904
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3592 5047 618
Size: 325 217 627 1805 1455 1842
Fragments arranged by size:
1842 1805 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2102 2116 2486 2774 3590 1391
Size: 711 14 370 288 816 4072
Fragments arranged by size:
4072 816 711 370 288 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2554 928
Size: 971 655 4645
Fragments arranged by size:
4645 971 655
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 2500 1548
Size: 952 5319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 4882 136
Size: 107 122 71 631 605 439 2771 1525
Fragments arranged by size:
2771 1525 631 605 439 122 107 71
BglII A'GATC_T
Cuts at: 6266 6266
Size: 6271
BlpI GC'TnA_GC
Cuts at: 2096 3688 2096
Size: 1592 4679
Bme1580I G_kGCm'C
Cuts at: 1790 2116 2944 4332 5488 1790
Size: 326 828 1388 1156 2573
Fragments arranged by size:
2573 1388 1156 828 326
BmgBI CAC'GTC
Cuts at: 1318 2128 1318
Size: 810 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 3318 4002
Size: 416 53 83 399 24 754 1147 684
Cuts at: 4002 4960 442
Size: 958 1753
Fragments arranged by size:
1753 1147 958 754 684 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2376 1056
Size: 1320 4951
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2444 2505 2595 2444
Size: 61 90 6120
Fragments arranged by size:
6120 90 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2259 2402 2826 2910 3078 3162 3246
Size: 917 103 143 424 84 168 84 84
Cuts at: 3246 4931 1239
Size: 1685 2579
Fragments arranged by size:
2579 1685 917 424 168 143 103 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4396 5261 5559 4396
Size: 865 298 5108
Fragments arranged by size:
5108 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 4934 882
Size: 617 662 2773 2219
Fragments arranged by size:
2773 2219 662 617
BsaAI yAC'GTr
Cuts at: 493 1204 2784 4062 493
Size: 711 1580 1278 2702
Fragments arranged by size:
2702 1580 1278 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4078 4460 5095 275
Size: 53 83 186 936 2545 382 635 1451
Fragments arranged by size:
2545 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3555 3585 1178
Size: 30 165 30 2152 30 3864
Fragments arranged by size:
3864 2152 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2764 1186
Size: 1578 4693
BseYI C'CCAG_C
Cuts at: 2196 5474 5970 2196
Size: 3278 496 2497
Fragments arranged by size:
3278 2497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2956 1163
Size: 573 1220 4478
Fragments arranged by size:
4478 1220 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2477 2486 2774 4332 4417 5488 2477
Size: 9 288 1558 85 1071 3260
Fragments arranged by size:
3260 1558 1071 288 85 9
BsmI GAATG_Cn'
Cuts at: 2828 3782 3875 2828
Size: 954 93 5224
Fragments arranged by size:
5224 954 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2116 2477 2486 2774 2944 3590
Size: 399 312 14 361 9 288 170 646
Cuts at: 3590 4332 4417 5488 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 646 399 361 312 288
170 85 14 9
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 3626 844
Size: 1184 1598 3489
Fragments arranged by size:
3489 1598 1184
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2396 3001 4766 4940 65
Size: 1183 1148 605 1765 174 1396
Fragments arranged by size:
1765 1396 1183 1148 605 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4915 5102 1700
Size: 110 3105 187 2869
Fragments arranged by size:
3105 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2118 96
Size: 2022 4249
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6271
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 2044 1184
Size: 860 5411
BstYI r'GATC_y
Cuts at: 1972 3601 3968 4353 4370 5822 5908 5920 6266
Size: 1629 367 385 17 1452 86 12 346
Cuts at: 6266 1972
Size: 1977
Fragments arranged by size:
1977 1629 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 513
Size: 552 939 24 4756
Fragments arranged by size:
4756 939 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 3787 4581 4601 3787
Size: 794 20 5457
Fragments arranged by size:
5457 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2815 2850 2983 3018 529
Size: 35 2251 35 133 35 3782
Fragments arranged by size:
3782 2251 133 35 35 35
DraI TTT'AAA
Cuts at: 2905 3575 2905
Size: 670 5601
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3718 4607 6236
Size: 54 1425 50 275 18 1888 889 1629
Cuts at: 6236 8
Size: 43
Fragments arranged by size:
1888 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2496 3115 4201 1353
Size: 723 420 619 1086 3423
Fragments arranged by size:
3423 1086 723 619 420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4858 5232 5378 387
Size: 1064 138 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2484 2772 2484
Size: 288 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2259 2328 2402 2826 2910 3020
Size: 393 524 103 69 74 424 84 110
Cuts at: 3020 3078 3082 3162 3246 3334 4334 4931 5718
Size: 58 4 80 84 88 1000 597 787
Cuts at: 5718 5986 1239
Size: 268 1524
Fragments arranged by size:
1524 1000 787 597 524 424 393 268
110 103 88 84 84 80 74 69
58 4
EcoO109I rG'GnC_Cy
Cuts at: 2112 2113 2174 2112
Size: 1 61 6209
Fragments arranged by size:
6209 61 1
EcoRI G'AATT_C
Cuts at: 2712 3048 3132 3300 3384 3580 3958 2712
Size: 336 84 168 84 196 378 5025
Fragments arranged by size:
5025 378 336 196 168 84 84
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 5418 1678
Size: 3740 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3179 3211 3445 3477 4912
Size: 32 135 32 1607 32 234 32 1435
Cuts at: 4912 4944 4986 5018 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1607 1435 234 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3615 3861 669
Size: 988 281 1677 246 3079
Fragments arranged by size:
3079 1677 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2841 3177 3513 748
Size: 2093 336 336 3506
Fragments arranged by size:
3506 2093 336 336
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5385 5569 1305
Size: 4080 184 2007
Fragments arranged by size:
4080 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4449
Fragments arranged by size:
4449 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4229 4588 4747 518
Size: 648 3063 359 159 2042
Fragments arranged by size:
3063 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2095 3687 4364 5512 5757 1606
Size: 489 1592 677 1148 245 2120
Fragments arranged by size:
2120 1592 1148 677 489 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2028 513
Size: 552 939 24 4756
Fragments arranged by size:
4756 939 552 24
NdeI CA'TA_TG
Cuts at: 387 3335 387
Size: 2948 3323
NheI G'CTAG_C
Cuts at: 1052 2372 1052
Size: 1320 4951
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 3330 3629 5174 1132
Size: 97 2101 299 1545 2229
Fragments arranged by size:
2229 2101 1545 299 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 3326 5170 3326
Size: 1844 4427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6271
PfoI T'CCnGG_A
Cuts at: 2034 2468 2034
Size: 434 5837
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2174 2174
Size: 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3156 3841 3156
Size: 685 5586
PspOMI G'GGCC_C
Cuts at: 2112 2112
Size: 6271
PstI C_TGCA'G
Cuts at: 830 3623 830
Size: 2793 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2095 2095
Size: 6271
SacI G_AGCT'C
Cuts at: 2486 2774 2486
Size: 288 5983
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 6271
SfcI C'TryA_G
Cuts at: 826 1045 2143 3619 4754 5435 5626 826
Size: 219 1098 1476 1135 681 191 1471
Fragments arranged by size:
1476 1471 1135 1098 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4375 5276 5538 820
Size: 197 926 2432 901 262 1553
Fragments arranged by size:
2432 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2784 4062 493
Size: 2291 1278 2702
Fragments arranged by size:
2702 2291 1278
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 3629 1132
Size: 2497 3774
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 2759 3095 1517
Size: 1242 336 4693
Fragments arranged by size:
4693 1242 336
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 3710 513
Size: 552 939 24 1682 3074
Fragments arranged by size:
3074 1682 939 552 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2090 4298 4315 4468 4653 5098 325
Size: 1255 510 2208 17 153 185 445 1498
Fragments arranged by size:
2208 1498 1255 510 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 3023 4517
Size: 274 80 33 51 1443 141 905 1494
Cuts at: 4517 6229 96
Size: 1712 138
Fragments arranged by size:
1712 1494 1443 905 274 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6231 6263 6231
Size: 32 6239
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 3095 3179 4400 3095
Size: 84 1221 4966
Fragments arranged by size:
4966 1221 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI