Restriction map for
pFN21AB7804
(Circular) (Six-base) MAPSORT of: pFN21AB7804.seq Check: 9135 from: 1 to: 6478
pFN21AB7804 6478 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3725 3725
Size: 6478
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4288 278
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2651 3821 1937
Size: 714 1170 4594
Fragments arranged by size:
4594 1170 714
Acc65I G'GTAC_C
Cuts at: 2891 3799 2891
Size: 908 5570
AclI AA'CG_TT
Cuts at: 1563 4605 4978 1563
Size: 3042 373 3063
Fragments arranged by size:
3063 3042 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 3227 4541 5925 6193 1632
Size: 692 903 1314 1384 268 1917
Fragments arranged by size:
1917 1384 1314 903 692 268
AfeI AGC'GCT
Cuts at: 3042 3042
Size: 6478
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6281
AflIII A'CryG_T
Cuts at: 1203 2697 5377 6354 1203
Size: 1494 2680 977 1327
Fragments arranged by size:
2680 1494 1327 977
AgeI A'CCGG_T
Cuts at: 5309 5309
Size: 6478
AhdI GACnn_n'nnGTC
Cuts at: 5207 5207
Size: 6478
AleI CACnn'nnGTG
Cuts at: 1166 3105 1166
Size: 1939 4539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6478
AlwNI CAG_nnn'CTG
Cuts at: 2493 2690 4221 5793 2493
Size: 197 1531 1572 3178
Fragments arranged by size:
3178 1572 1531 197
ApaLI G'TGCA_C
Cuts at: 4535 5691 4535
Size: 1156 5322
ApoI r'AATT_y
Cuts at: 1493 2325 2570 3313 3787 4013 4165 6274 1493
Size: 832 245 743 474 226 152 2109 1697
Fragments arranged by size:
2109 1697 832 743 474 245 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5032 160
Size: 872 4000 1606
Fragments arranged by size:
4000 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
AvaI C'yCGr_G
Cuts at: 1870 1943 2154 2809 3616 3803 1870
Size: 73 211 655 807 187 4545
Fragments arranged by size:
4545 807 655 211 187 73
BamHI G'GATC_C
Cuts at: 3808 4175 3808
Size: 367 6111
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2891 3099 3799 5254
Size: 325 217 627 315 789 208 700 1455
Cuts at: 5254 618
Size: 1842
Fragments arranged by size:
1842 1455 789 700 627 325 315 217
208
BanII G_rGCy'C
Cuts at: 1391 2161 2737 3797 1391
Size: 770 576 1060 4072
Fragments arranged by size:
4072 1060 770 576
BbeI G_GCGC'C
Cuts at: 2106 2106
Size: 6478
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4669 4703 1903
Size: 34 2732 34 3678
Fragments arranged by size:
3678 2732 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4370 5580 3467
Size: 903 1210 4365
Fragments arranged by size:
4365 1210 903
BclI T'GATC_A
Cuts at: 1548 2844 2916 1548
Size: 1296 72 5110
Fragments arranged by size:
5110 1296 72
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 5089 136
Size: 107 122 71 631 605 454 2963 1525
Fragments arranged by size:
2963 1525 631 605 454 122 107 71
BglII A'GATC_T
Cuts at: 6473 6473
Size: 6478
BlpI GC'TnA_GC
Cuts at: 3529 3895 3529
Size: 366 6112
Bme1580I G_kGCm'C
Cuts at: 1790 4539 5695 1790
Size: 2749 1156 2573
Fragments arranged by size:
2749 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2091 1318
Size: 773 5705
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4209 5167 442
Size: 416 53 83 399 24 2792 958 1753
Fragments arranged by size:
2792 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6478
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2483 2551 3444 2483
Size: 68 893 5517
Fragments arranged by size:
5517 893 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2926 5138 1239
Size: 1687 2212 2579
Fragments arranged by size:
2579 2212 1687
Bpu10I CC'TnA_GC
Cuts at: 3114 3114
Size: 6478
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3373 4603 5468 5766 3373
Size: 1230 865 298 4085
Fragments arranged by size:
4085 1230 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3655 5141 882
Size: 617 2156 1486 2219
Fragments arranged by size:
2219 2156 1486 617
BsaAI yAC'GTr
Cuts at: 493 1204 2386 2621 3415 4269 493
Size: 711 1182 235 794 854 2702
Fragments arranged by size:
2702 1182 854 794 711 235
BsaBI GATnn'nnATC
Cuts at: 6472 6472
Size: 6478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2103 2118 4285 4667
Size: 53 83 186 936 570 15 2167 382
Cuts at: 4667 5302 275
Size: 635 1451
Fragments arranged by size:
2167 1451 936 635 570 382 186 83
53 15
BsaWI w'CCGG_w
Cuts at: 4911 5309 5583 5730 6141 4911
Size: 398 274 147 411 5248
Fragments arranged by size:
5248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3434 3464 1178
Size: 30 165 30 2031 30 4192
Fragments arranged by size:
4192 2031 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2913 2940 3571 3604 1186
Size: 1727 27 631 33 4060
Fragments arranged by size:
4060 1727 631 33 27
BseYI C'CCAG_C
Cuts at: 2062 3432 5681 6177 2062
Size: 1370 2249 496 2363
Fragments arranged by size:
2363 2249 1370 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2506 2960 3703 1163
Size: 573 770 454 743 3938
Fragments arranged by size:
3938 770 743 573 454
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3219 3928 4689 5717 1540
Size: 275 186 1218 709 761 1028 2301
Fragments arranged by size:
2301 1218 1028 761 709 275 186
BsiHKAI G_wGCw'C
Cuts at: 3425 4539 4624 5695 3425
Size: 1114 85 1071 4208
Fragments arranged by size:
4208 1114 1071 85
BsmI GAATG_Cn'
Cuts at: 2611 3989 4082 2611
Size: 1378 93 5007
Fragments arranged by size:
5007 1378 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 2737 3425 3797 4539 4624 5695
Size: 399 371 576 688 372 742 85 1071
Cuts at: 5695 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 688 576 399 372 371
85
BspEI T'CCGG_A
Cuts at: 6141 6141
Size: 6478
BspHI T'CATG_A
Cuts at: 4367 6097 4367
Size: 1730 4748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3725 3833 844
Size: 2881 108 3489
Fragments arranged by size:
3489 2881 108
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 3215 4365 1394
Size: 429 316 1076 1150 3507
Fragments arranged by size:
3507 1150 1076 429 316
BsrDI GCAATG_nn'
Cuts at: 65 1248 2362 3222 3495 4973 5147 65
Size: 1183 1114 860 273 1478 174 1396
Fragments arranged by size:
1478 1396 1183 1114 860 273 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2334 5122 5309 1700
Size: 110 524 2788 187 2869
Fragments arranged by size:
2869 2788 524 187 110
BsrGI T'GTAC_A
Cuts at: 96 3237 96
Size: 3141 3337
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6478
BssSI C'ACGA_G
Cuts at: 3460 4538 5550 3460
Size: 1078 1012 4388
Fragments arranged by size:
4388 1078 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6478
BstBI TT'CG_AA
Cuts at: 5282 5282
Size: 6478
BstEII G'GTnAC_C
Cuts at: 1184 3107 1184
Size: 1923 4555
BstXI CCAn_nnnn'nTGG
Cuts at: 2036 2036
Size: 6478
BstYI r'GATC_y
Cuts at: 1972 2539 3154 3808 4175 4560 4577 6029 6115
Size: 567 615 654 367 385 17 1452 86
Cuts at: 6115 6127 6473 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 654 615 567 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2247 3573 513
Size: 552 1182 1326 3418
Fragments arranged by size:
3418 1326 1182 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5739
Fragments arranged by size:
5739 683 56
BtsI GCAGTG_nn'
Cuts at: 3994 4788 4808 3994
Size: 794 20 5664
Fragments arranged by size:
5664 794 20
ClaI AT'CG_AT
Cuts at: 1554 3060 1554
Size: 1506 4972
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6443
DraI TTT'AAA
Cuts at: 3782 3782
Size: 6478
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5485 809
Size: 507 4169 1802
Fragments arranged by size:
4169 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 3216 3333
Size: 54 1425 50 275 18 841 545 117
Cuts at: 3333 3925 4814 6443 8
Size: 592 889 1629 43
Fragments arranged by size:
1629 1425 889 841 592 545 275 117
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3216 3925 1537
Size: 275 1404 709 4090
Fragments arranged by size:
4090 1404 709 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 3203 3662 4408 1353
Size: 696 1154 459 746 3423
Fragments arranged by size:
3423 1154 746 696 459
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2743 5065 5439 5585 387
Size: 1064 138 1154 2322 374 146 1280
Fragments arranged by size:
2322 1280 1154 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2324 2926 3227 4541 5138 5925 6193
Size: 393 692 602 301 1314 597 787 268
Cuts at: 6193 1239
Size: 1524
Fragments arranged by size:
1524 1314 787 692 602 597 393 301
268
EcoO109I rG'GnC_Cy
Cuts at: 3232 3232
Size: 6478
EcoRI G'AATT_C
Cuts at: 2325 3313 3787 4165 2325
Size: 988 474 378 4638
Fragments arranged by size:
4638 988 474 378
EcoRV GAT'ATC
Cuts at: 3753 3753
Size: 6478
FspI TGC'GCA
Cuts at: 4984 4984
Size: 6478
HaeII r_GCGC'y
Cuts at: 1678 2106 3044 5625 1678
Size: 428 938 2581 2531
Fragments arranged by size:
2581 2531 938 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2625 2657 2908 2935 2940
Size: 32 135 32 1053 32 251 27 5
Cuts at: 2940 2967 3754 3786 5119 5151 5193 5225 1373
Size: 27 787 32 1333 32 42 32 2626
Fragments arranged by size:
2626 1333 1053 787 251 135 42 32
32 32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3822 4068 669
Size: 988 281 1884 246 3079
Fragments arranged by size:
3079 1884 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6478
HpaI GTT'AAC
Cuts at: 4068 4068
Size: 6478
KasI G'GCGC_C
Cuts at: 2102 2102
Size: 6478
KpnI G_GTAC'C
Cuts at: 2895 3803 2895
Size: 908 5570
MfeI C'AATT_G
Cuts at: 4077 4077
Size: 6478
MluI A'CGCG_T
Cuts at: 6354 6354
Size: 6478
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2053 2223 3624 5592 5776 1305
Size: 748 170 1401 1968 184 2007
Fragments arranged by size:
2007 1968 1401 748 184 170
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2673 10
Size: 54 1425 343 841 3815
Fragments arranged by size:
3815 1425 841 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2936 3105 3464 4436 4795 4954 518
Size: 648 1770 169 359 972 359 159 2042
Fragments arranged by size:
2042 1770 972 648 359 359 169 159
MspA1I CmG'CkG
Cuts at: 1606 2062 2070 2493 3533 3894 4571 5719 5964
Size: 456 8 423 1040 361 677 1148 245
Cuts at: 5964 1606
Size: 2120
Fragments arranged by size:
2120 1148 1040 677 456 423 361 245
8
NarI GG'CG_CC
Cuts at: 2103 2103
Size: 6478
NcoI C'CATG_G
Cuts at: 513 1065 3573 513
Size: 552 2508 3418
Fragments arranged by size:
3418 2508 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6478
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6478
NotI GC'GGCC_GC
Cuts at: 3925 3925
Size: 6478
NspI r_CATG'y
Cuts at: 1132 1229 2629 2701 2783 3836 5381 1132
Size: 97 1400 72 82 1053 1545 2229
Fragments arranged by size:
2229 1545 1400 1053 97 82 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6478
PciI A'CATG_T
Cuts at: 2697 5377 2697
Size: 2680 3798
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 2644 2680 1108
Size: 1140 396 36 4906
Fragments arranged by size:
4906 1140 396 36
PmeI GTTT'AAAC
Cuts at: 3782 3782
Size: 6478
PmlI CAC'GTG
Cuts at: 2621 2621
Size: 6478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4547 4579 6060 6092 4547
Size: 32 1481 32 4933
Fragments arranged by size:
4933 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6478
PsiI TTA'TAA
Cuts at: 4048 4048
Size: 6478
PstI C_TGCA'G
Cuts at: 830 3608 3830 830
Size: 2778 222 3478
Fragments arranged by size:
3478 2778 222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6478
PvuII CAG'CTG
Cuts at: 3533 3533
Size: 6478
SalI G'TCGA_C
Cuts at: 1936 3820 1936
Size: 1884 4594
SapI GCTCTTCn'nnn_
Cuts at: 2049 2049
Size: 6478
SbfI CC_TGCA'GG
Cuts at: 3830 3830
Size: 6478
ScaI AGT'ACT
Cuts at: 4726 6438 4726
Size: 1712 4766
SfcI C'TryA_G
Cuts at: 826 1045 2350 3604 3826 4961 5642 5833 826
Size: 219 1305 1254 222 1135 681 191 1471
Fragments arranged by size:
1471 1305 1254 1135 681 222 219 191
SfoI GGC'GCC
Cuts at: 2104 2104
Size: 6478
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6478
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6478
SmaI CCC'GGG
Cuts at: 1872 2156 3805 1872
Size: 284 1649 4545
Fragments arranged by size:
4545 1649 284
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3388 4582 5483 5745 820
Size: 197 926 1445 1194 901 262 1553
Fragments arranged by size:
1553 1445 1194 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4269 493
Size: 3776 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6478
SphI G_CATG'C
Cuts at: 1132 3836 1132
Size: 2704 3774
SrfI GCCC'GGGC
Cuts at: 2156 2156
Size: 6478
SspI AAT'ATT
Cuts at: 5 52 4402 5
Size: 47 4350 2081
Fragments arranged by size:
4350 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6478
StyI C'CwwG_G
Cuts at: 513 1065 3573 3917 513
Size: 552 2508 344 3074
Fragments arranged by size:
3074 2508 552 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3277 4505 4522 4675 4860 5305 325
Size: 1255 1697 1228 17 153 185 445 1498
Fragments arranged by size:
1697 1498 1255 1228 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3237 4724 6436
Size: 274 80 33 51 1443 1260 1487 1712
Cuts at: 6436 96
Size: 138
Fragments arranged by size:
1712 1487 1443 1260 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6438 6470 6438
Size: 32 6446
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6478
XbaI T'CTAG_A
Cuts at: 3814 3814
Size: 6478
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3571 3571
Size: 6478
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6478
XmaI C'CCGG_G
Cuts at: 1870 2154 3803 1870
Size: 284 1649 4545
Fragments arranged by size:
4545 1649 284
XmnI GAAnn'nnTTC
Cuts at: 2573 3201 3317 4607 2573
Size: 628 116 1290 4444
Fragments arranged by size:
4444 1290 628 116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4286 276
Size: 53 83 186 936 2752 2468
Fragments arranged by size:
2752 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SwaI