Restriction map for
pFN21AB7801
(Circular) (Six-base) MAPSORT of: pFN21AB7801.seq Check: 4253 from: 1 to: 6262
pFN21AB7801 6262 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2607 3143 2607
Size: 536 5726
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2219 4072 278
Size: 53 83 186 936 683 1853 2468
Fragments arranged by size:
2468 1853 936 683 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2351 3605 1937
Size: 414 1254 4594
Fragments arranged by size:
4594 1254 414
Acc65I G'GTAC_C
Cuts at: 2343 3583 2343
Size: 1240 5022
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2665 2906 3038 3056 4325 5709 5977 1632
Size: 1033 241 132 18 1269 1384 268 1917
Fragments arranged by size:
1917 1384 1269 1033 268 241 132 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 5161 6138 1203
Size: 3958 977 1327
Fragments arranged by size:
3958 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 2634 1166
Size: 1468 4794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AlwNI CAG_nnn'CTG
Cuts at: 2158 3487 4005 5577 2158
Size: 1329 518 1572 2843
Fragments arranged by size:
2843 1572 1329 518
ApaI G_GGCC'C
Cuts at: 2278 2728 3201 2278
Size: 450 473 5339
Fragments arranged by size:
5339 473 450
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 2892 3571 3797 3949 6058 1493
Size: 1399 679 226 152 2109 1697
Fragments arranged by size:
2109 1697 1399 679 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2271 2721 3331 3587 1870
Size: 73 132 196 450 610 256 4545
Fragments arranged by size:
4545 610 450 256 196 132 73
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2224 2343 3131 3583 5038
Size: 325 217 627 437 119 788 452 1455
Cuts at: 5038 618
Size: 1842
Fragments arranged by size:
1842 1455 788 627 452 437 325 217
119
BanII G_rGCy'C
Cuts at: 1391 2076 2278 2728 3201 3255 3338 3581 1391
Size: 685 202 450 473 54 83 243 4072
Fragments arranged by size:
4072 685 473 450 243 202 83 54
BbeI G_GCGC'C
Cuts at: 2228 2228
Size: 6262
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5291
BbvCI CC'TCA_GC
Cuts at: 2829 2829
Size: 6262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4873 136
Size: 107 122 71 631 605 3201 1525
Fragments arranged by size:
3201 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6257 6257
Size: 6262
BlpI GC'TnA_GC
Cuts at: 2057 2241 3679 2057
Size: 184 1438 4640
Fragments arranged by size:
4640 1438 184
Bme1580I G_kGCm'C
Cuts at: 1790 2278 2728 3201 4323 5479 1790
Size: 488 450 473 1122 1156 2573
Fragments arranged by size:
2573 1156 1122 488 473 450
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2272 2502 2582
Size: 416 53 83 399 24 855 230 80
Cuts at: 2582 3993 4951 442
Size: 1411 958 1753
Fragments arranged by size:
1753 1411 958 855 416 399 230 83
80 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2558 2692 2725 2987 3047 3416 3473
Size: 797 522 134 33 262 60 369 57
Cuts at: 3473 3524 4922 1239
Size: 51 1398 2579
Fragments arranged by size:
2579 1398 797 522 369 262 134 60
57 51 33
Bpu10I CC'TnA_GC
Cuts at: 2829 2925 3380 2829
Size: 96 455 5711
Fragments arranged by size:
5711 455 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4387 5252 5550 4387
Size: 865 298 5099
Fragments arranged by size:
5099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3437 4925 882
Size: 617 1938 1488 2219
Fragments arranged by size:
2219 1938 1488 617
BsaAI yAC'GTr
Cuts at: 493 1204 4053 493
Size: 711 2849 2702
Fragments arranged by size:
2849 2702 711
BsaBI GATnn'nnATC
Cuts at: 3414 6256 3414
Size: 2842 3420
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2216 2225 4069 4451
Size: 53 83 186 936 683 9 1844 382
Cuts at: 4451 5086 275
Size: 635 1451
Fragments arranged by size:
1844 1451 936 683 635 382 186 83
53 9
BsaWI w'CCGG_w
Cuts at: 4695 5093 5367 5514 5925 4695
Size: 398 274 147 411 5032
Fragments arranged by size:
5032 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2237 2267 2690 2720 3248
Size: 30 165 30 834 30 423 30 528
Cuts at: 3248 3278 1178
Size: 30 4162
Fragments arranged by size:
4162 834 528 423 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 2466 2744 2754 2819 2822 2888 3332
Size: 1093 187 278 10 65 3 66 444
Cuts at: 3332 1186
Size: 4116
Fragments arranged by size:
4116 1093 444 278 187 66 65 10
3
BseYI C'CCAG_C
Cuts at: 3230 3341 3349 3357 5465 5961 3230
Size: 111 8 8 2108 496 3531
Fragments arranged by size:
3531 2108 496 111 8 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 2700 1163
Size: 573 921 43 4725
Fragments arranged by size:
4725 921 573 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 4323 4408 5479 2247
Size: 2076 85 1071 3030
Fragments arranged by size:
3030 2076 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3238 3773 3866 3170
Size: 68 535 93 5566
Fragments arranged by size:
5566 535 93 68
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2247 2278 2728 3201 3255 3338
Size: 399 286 171 31 450 473 54 83
Cuts at: 3338 3581 4323 4408 5479 1391
Size: 243 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 473 450 399 286 243
171 85 83 54 31
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 2545 4151 5881 2545
Size: 1606 1730 2926
Fragments arranged by size:
2926 1730 1606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 3113 3143 3294 3617 844
Size: 1763 506 30 151 323 3489
Fragments arranged by size:
3489 1763 506 323 151 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2022 4149 1394
Size: 429 199 2127 3507
Fragments arranged by size:
3507 2127 429 199
BsrDI GCAATG_nn'
Cuts at: 65 1248 4757 4931 65
Size: 1183 3509 174 1396
Fragments arranged by size:
3509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4906 5093 1700
Size: 110 3096 187 2869
Fragments arranged by size:
3096 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 2506 4322 5334 2506
Size: 1816 1012 3434
Fragments arranged by size:
3434 1816 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2035 3244 794
Size: 1241 1209 3812
Fragments arranged by size:
3812 1241 1209
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 3220 2458
Size: 762 5500
BstYI r'GATC_y
Cuts at: 1972 3275 3415 3592 3959 4344 4361 5813 5899
Size: 1303 140 177 367 385 17 1452 86
Cuts at: 5899 5911 6257 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1303 385 367 346 177 140
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3267 513
Size: 552 939 1263 3508
Fragments arranged by size:
3508 1263 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 2048 2641 3778 4572 4592 2048
Size: 593 1137 794 20 3718
Fragments arranged by size:
3718 1137 794 593 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2181 2216 529
Size: 35 1617 35 4575
Fragments arranged by size:
4575 1617 35 35
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3709 4598 6227
Size: 54 1425 50 275 18 1879 889 1629
Cuts at: 6227 8
Size: 43
Fragments arranged by size:
1879 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 2807 3538 4192 1353
Size: 824 630 731 654 3423
Fragments arranged by size:
3423 824 731 654 630
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2722 4849 5223 5369 387
Size: 1064 138 1133 2127 374 146 1280
Fragments arranged by size:
2127 1280 1133 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2558 2665 2692 2725 2906 2987
Size: 393 404 522 107 27 33 181 81
Cuts at: 2987 3038 3047 3056 3416 3473 3524 4325 4922
Size: 51 9 9 360 57 51 801 597
Cuts at: 4922 5709 5977 1239
Size: 787 268 1524
Fragments arranged by size:
1524 801 787 597 522 404 393 360
268 181 107 81 57 51 51 33
27 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2801 2261
Size: 540 5722
EcoO109I rG'GnC_Cy
Cuts at: 2089 2725 2786 2817 2881 3198 2089
Size: 636 61 31 64 317 5153
Fragments arranged by size:
5153 636 317 64 61 31
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 2228 3498 5409 1678
Size: 550 1270 1911 2531
Fragments arranged by size:
2531 1911 1270 550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2908 2940 3308 3340 4903
Size: 32 135 32 1336 32 368 32 1563
Cuts at: 4903 4935 4977 5009 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1563 1336 368 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3068 748
Size: 2320 3942
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KasI G'GCGC_C
Cuts at: 2224 2224
Size: 6262
KpnI G_GTAC'C
Cuts at: 2347 3587 2347
Size: 1240 5022
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 5376 5560 1305
Size: 1134 2937 184 2007
Fragments arranged by size:
2937 2007 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4440
Fragments arranged by size:
4440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2634 3146 3218 4220 4579 4738
Size: 648 843 625 512 72 1002 359 159
Cuts at: 4738 518
Size: 2042
Fragments arranged by size:
2042 1002 843 648 625 512 359 159
72
MspA1I CmG'CkG
Cuts at: 1606 3678 4355 5503 5748 1606
Size: 2072 677 1148 245 2120
Fragments arranged by size:
2120 2072 1148 677 245
NarI GG'CG_CC
Cuts at: 2225 2225
Size: 6262
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4771
Fragments arranged by size:
4771 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 3620 5165 1132
Size: 97 2391 1545 2229
Fragments arranged by size:
2391 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 2817
Size: 6262
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6262
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 2274 2724 3197 2274
Size: 450 473 5339
Fragments arranged by size:
5339 473 450
PstI C_TGCA'G
Cuts at: 830 2043 2778 3249 3480 3614 830
Size: 1213 735 471 231 134 3478
Fragments arranged by size:
3478 1213 735 471 231 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3249 3614 3249
Size: 365 5897
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SexAI A'CCwGG_T
Cuts at: 3486 3486
Size: 6262
SfcI C'TryA_G
Cuts at: 826 1045 2039 2352 2774 3245 3476 3610 4745
Size: 219 994 313 422 471 231 134 1135
Cuts at: 4745 5426 5617 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 994 681 471 422 313 231
219 191 134
SfoI GGC'GCC
Cuts at: 2226 2226
Size: 6262
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 2077 2723 3333 3589 1872
Size: 205 646 610 256 4545
Fragments arranged by size:
4545 646 610 256 205
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4366 5267 5529 820
Size: 197 926 2423 901 262 1553
Fragments arranged by size:
2423 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SrfI GCCC'GGGC
Cuts at: 3333 3333
Size: 6262
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 2004 3701 513
Size: 552 939 1697 3074
Fragments arranged by size:
3074 1697 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 4289 4306 4459 4644 5089 325
Size: 1255 726 1983 17 153 185 445 1498
Fragments arranged by size:
1983 1498 1255 726 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4508 6220 96
Size: 274 80 33 51 1443 2531 1712 138
Fragments arranged by size:
2531 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2237 2269 2690 2722 6222 6254 2237
Size: 32 421 32 3500 32 2245
Fragments arranged by size:
3500 2245 421 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6262
XbaI T'CTAG_A
Cuts at: 3028 3598 3028
Size: 570 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3409 2011
Size: 1398 4864
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 2075 2721 3331 3587 1870
Size: 205 646 610 256 4545
Fragments arranged by size:
4545 646 610 256 205
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2217 4070 276
Size: 53 83 186 936 683 1853 2468
Fragments arranged by size:
2468 1853 936 683 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SwaI