Restriction map for
pFN21AB7796
(Circular) (Six-base) MAPSORT of: pFN21AB7796.seq Check: 7686 from: 1 to: 6259
pFN21AB7796 6259 bp
With 182 enzymes: SgfI *
December 8, 2011 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3602 1937
Size: 1665 4594
Acc65I G'GTAC_C
Cuts at: 2026 3580 2026
Size: 1554 4705
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2657 4322 5706 5974 1632
Size: 806 219 1665 1384 268 1917
Fragments arranged by size:
1917 1665 1384 806 268 219
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 5158 6135 1203
Size: 3955 977 1327
Fragments arranged by size:
3955 1327 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2981 1184
Size: 1797 4462
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2613 2645 2613
Size: 32 6227
AlwNI CAG_nnn'CTG
Cuts at: 2983 3026 4002 5574 2983
Size: 43 976 1572 3668
Fragments arranged by size:
3668 1572 976 43
ApaLI G'TGCA_C
Cuts at: 2966 4316 5472 2966
Size: 1350 1156 3753
Fragments arranged by size:
3753 1350 1156
ApoI r'AATT_y
Cuts at: 1493 3568 3794 3946 6055 1493
Size: 2075 226 152 2109 1697
Fragments arranged by size:
2109 2075 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 3584 1870
Size: 73 1641 4545
Fragments arranged by size:
4545 1641 73
BamHI G'GATC_C
Cuts at: 3589 3956 3589
Size: 367 5892
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 3106 3184 3412 3580
Size: 325 217 627 239 1080 78 228 168
Cuts at: 3580 5035 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1080 627 325 239 228 217
168 78
BanII G_rGCy'C
Cuts at: 1391 2606 3061 3578 1391
Size: 1215 455 517 4072
Fragments arranged by size:
4072 1215 517 455
BbeI G_GCGC'C
Cuts at: 3110 3110
Size: 6259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2128 928
Size: 971 229 5059
Fragments arranged by size:
5059 971 229
BbvCI CC'TCA_GC
Cuts at: 2976 2976
Size: 6259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2020 2054 4450 4484 1903
Size: 34 83 34 2396 34 3678
Fragments arranged by size:
3678 2396 83 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3393 4870
Size: 107 122 71 631 605 337 1384 1477
Cuts at: 4870 136
Size: 1525
Fragments arranged by size:
1525 1477 1384 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 2430 2451 2970 3189 3417 4320 5476 1790
Size: 640 21 519 219 228 903 1156 2573
Fragments arranged by size:
2573 1156 903 640 519 228 219 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 3990 4948
Size: 416 53 83 399 24 1754 819 958
Cuts at: 4948 442
Size: 1753
Fragments arranged by size:
1754 1753 958 819 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2705 2714 3058 4919 1239
Size: 1466 9 344 1861 2579
Fragments arranged by size:
2579 1861 1466 344 9
Bpu10I CC'TnA_GC
Cuts at: 2976 2976
Size: 6259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4384 5249 5547 4384
Size: 865 298 5096
Fragments arranged by size:
5096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 4922 882
Size: 617 774 2649 2219
Fragments arranged by size:
2649 2219 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 4050 493
Size: 711 2846 2702
Fragments arranged by size:
2846 2702 711
BsaBI GATnn'nnATC
Cuts at: 3161 6253 3161
Size: 3092 3167
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3107 3422 4066 4448
Size: 53 83 186 936 1574 315 644 382
Cuts at: 4448 5083 275
Size: 635 1451
Fragments arranged by size:
1574 1451 936 644 635 382 315 186
83 53
BsaWI w'CCGG_w
Cuts at: 2338 4692 5090 5364 5511 5922 2338
Size: 2354 398 274 147 411 2675
Fragments arranged by size:
2675 2354 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 2613 2643 3056
Size: 30 165 30 867 30 313 30 413
Cuts at: 3056 3086 1178
Size: 30 4351
Fragments arranged by size:
4351 867 413 313 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 2516 2519 2702 2756 3138 1186
Size: 1189 141 3 183 54 382 4307
Fragments arranged by size:
4307 1189 382 183 141 54 3
BseYI C'CCAG_C
Cuts at: 2125 2394 5462 5958 2125
Size: 269 3068 496 2426
Fragments arranged by size:
3068 2426 496 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 1163
Size: 573 1065 4621
Fragments arranged by size:
4621 1065 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2045 2676 3709 4470 5498 1540
Size: 275 186 44 631 1033 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 631 275 186 44
BsiHKAI G_wGCw'C
Cuts at: 2478 2970 4320 4405 5476 2478
Size: 492 1350 85 1071 3261
Fragments arranged by size:
3261 1350 1071 492 85
BsmI GAATG_Cn'
Cuts at: 3770 3863 3770
Size: 93 6166
BsmBI CGTCTCn'nnnn_
Cuts at: 2257 2257
Size: 6259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 2451 2478 2606 2970 3061 3189
Size: 399 640 21 27 128 364 91 128
Cuts at: 3189 3417 3578 4320 4405 5476 1391
Size: 228 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 399 364 228 161
128 128 91 85 27 21
BspEI T'CCGG_A
Cuts at: 2338 5922 2338
Size: 3584 2675
BspHI T'CATG_A
Cuts at: 3093 4148 5878 3093
Size: 1055 1730 3474
Fragments arranged by size:
3474 1730 1055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2773 3614 844
Size: 1929 841 3489
Fragments arranged by size:
3489 1929 841
BsrBI CCG'CTC
Cuts at: 1394 1823 4146 1394
Size: 429 2323 3507
Fragments arranged by size:
3507 2323 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4754 4928 65
Size: 1183 3506 174 1396
Fragments arranged by size:
3506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2589 2882 2970 3347 3371 4903
Size: 110 234 545 293 88 377 24 1532
Cuts at: 4903 5090 1700
Size: 187 2869
Fragments arranged by size:
2869 1532 545 377 293 234 187 110
88 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6259
BssSI C'ACGA_G
Cuts at: 4319 5331 4319
Size: 1012 5247
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3026 794
Size: 2232 4027
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstXI CCAn_nnnn'nTGG
Cuts at: 2329 2839 3544 2329
Size: 510 705 5044
Fragments arranged by size:
5044 705 510
BstYI r'GATC_y
Cuts at: 1972 2400 3589 3956 4341 4358 5810 5896 5908
Size: 428 1189 367 385 17 1452 86 12
Cuts at: 5908 6254 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1189 428 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3150 3150
Size: 6259
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 2242 3366 513
Size: 552 939 193 45 1124 3406
Fragments arranged by size:
3406 1124 939 552 193 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5520
Fragments arranged by size:
5520 683 56
BtsI GCAGTG_nn'
Cuts at: 3775 4569 4589 3775
Size: 794 20 5445
Fragments arranged by size:
5445 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2083 2118 529
Size: 35 1519 35 4670
Fragments arranged by size:
4670 1519 35 35
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2042 2104 2673
Size: 54 1425 50 275 18 212 62 569
Cuts at: 2673 3243 3369 3385 3394 3706 4595 6224 8
Size: 570 126 16 9 312 889 1629 43
Fragments arranged by size:
1629 1425 889 570 569 312 275 212
126 62 54 50 43 18 16 9
EagI C'GGCC_G
Cuts at: 1537 1812 2042 2673 3706 1537
Size: 275 230 631 1033 4090
Fragments arranged by size:
4090 1033 631 275 230
EarI CTCTTCn'nnn_
Cuts at: 1353 4189 1353
Size: 2836 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2159 4846 5220 5366 387
Size: 1064 138 570 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 570 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2657 2705 2714 3058 4322 4919
Size: 393 806 219 48 9 344 1264 597
Cuts at: 4919 5706 5974 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1264 806 787 597 393 344 268
219 48 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2707 2707
Size: 6259
EcoO109I rG'GnC_Cy
Cuts at: 2712 3456 2712
Size: 744 5515
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 3110 5406 1678
Size: 1432 2296 2531
Fragments arranged by size:
2531 2296 1432
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2588 2600 2620
Size: 32 135 32 698 32 286 12 20
Cuts at: 2620 2632 3029 3061 4900 4932 4974 5006 1373
Size: 12 397 32 1839 32 42 32 2626
Fragments arranged by size:
2626 1839 698 397 286 135 42 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3603 3849 669
Size: 988 281 1665 246 3079
Fragments arranged by size:
3079 1665 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6259
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KasI G'GCGC_C
Cuts at: 3106 3106
Size: 6259
KpnI G_GTAC'C
Cuts at: 2030 3584 2030
Size: 1554 4705
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 2639 3138 5373 5557 1305
Size: 1293 41 499 2235 184 2007
Fragments arranged by size:
2235 2007 1293 499 184 41
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2106 10
Size: 54 1425 343 274 4163
Fragments arranged by size:
4163 1425 343 274 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2885 4217 4576 4735 518
Size: 648 1719 1332 359 159 2042
Fragments arranged by size:
2042 1719 1332 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2230 2394 2683 2839 2911 3023 3223
Size: 519 105 164 289 156 72 112 200
Cuts at: 3223 3264 3274 3675 4352 5500 5745 1606
Size: 41 10 401 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 519 401 289 245 200
164 156 112 105 72 41 10
NaeI GCC'GGC
Cuts at: 2046 3373 2046
Size: 1327 4932
NarI GG'CG_CC
Cuts at: 3107 3107
Size: 6259
NcoI C'CATG_G
Cuts at: 513 1065 2004 2197 513
Size: 552 939 193 4575
Fragments arranged by size:
4575 939 552 193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NgoMIV G'CCGG_C
Cuts at: 2044 3371 2044
Size: 1327 4932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 2389 2890 3617 5162 1132
Size: 97 1160 501 727 1545 2229
Fragments arranged by size:
2229 1545 1160 727 501 97
PasI CC'CwG_GG
Cuts at: 1348 3417 3453 1348
Size: 2069 36 4154
Fragments arranged by size:
4154 2069 36
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6259
PfoI T'CCnGG_A
Cuts at: 2403 2403
Size: 6259
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2613 2645 4328 4360 5841 5873 2613
Size: 32 1683 32 1481 32 2999
Fragments arranged by size:
2999 1683 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2712 2712
Size: 6259
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6259
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PstI C_TGCA'G
Cuts at: 830 3022 3031 3269 3611 830
Size: 2192 9 238 342 3478
Fragments arranged by size:
3478 2192 342 238 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 2125 2230 2394 2683 2839 2911 3264 2125
Size: 105 164 289 156 72 353 5120
Fragments arranged by size:
5120 353 289 164 156 105 72
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SbfI CC_TGCA'GG
Cuts at: 3611 3611
Size: 6259
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 3018 3027 3265 3337 3352 3607 4742
Size: 219 1973 9 238 72 15 255 1135
Cuts at: 4742 5423 5614 826
Size: 681 191 1471
Fragments arranged by size:
1973 1471 1135 681 255 238 219 191
72 15 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3393 3393
Size: 6259
SfoI GGC'GCC
Cuts at: 3108 3108
Size: 6259
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 3586 1872
Size: 1714 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4363 5264 5526 820
Size: 197 926 2420 901 262 1553
Fragments arranged by size:
2420 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 2890 3617 1132
Size: 1758 727 3774
Fragments arranged by size:
3774 1758 727
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 2702 3320 1517
Size: 1185 618 4456
Fragments arranged by size:
4456 1185 618
StyI C'CwwG_G
Cuts at: 513 1065 2004 2107 2197 3698 513
Size: 552 939 103 90 1501 3074
Fragments arranged by size:
3074 1501 939 552 103 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2451 2893 3346 4286 4303 4456 4641
Size: 1255 871 442 453 940 17 153 185
Cuts at: 4641 5086 325
Size: 445 1498
Fragments arranged by size:
1498 1255 940 871 453 445 442 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2364 4505 6217
Size: 274 80 33 51 1443 387 2141 1712
Cuts at: 6217 96
Size: 138
Fragments arranged by size:
2141 1712 1443 387 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 6219 6251 3056
Size: 32 3131 32 3064
Fragments arranged by size:
3131 3064 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2284 2655 1316
Size: 968 371 4920
Fragments arranged by size:
4920 968 371
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3517 3517
Size: 6259
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6259
XmaI C'CCGG_G
Cuts at: 1870 3584 1870
Size: 1714 4545
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SrfI SwaI