Restriction map for
pFN21AB7794
(Circular) (Six-base) MAPSORT of: pFN21AB7794.seq Check: 8204 from: 1 to: 6262
pFN21AB7794 6262 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3411 3411
Size: 6262
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3246 3605 1937
Size: 1309 359 4594
Fragments arranged by size:
4594 1309 359
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4325 5709 5977 1632
Size: 2693 1384 268 1917
Fragments arranged by size:
2693 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 5161 6138 1203
Size: 3958 977 1327
Fragments arranged by size:
3958 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3163 3344 3347 1184
Size: 1979 181 3 4099
Fragments arranged by size:
4099 1979 181 3
AlwNI CAG_nnn'CTG
Cuts at: 3052 3070 4005 5577 3052
Size: 18 935 1572 3737
Fragments arranged by size:
3737 1572 935 18
ApaI G_GGCC'C
Cuts at: 3539 3539
Size: 6262
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 3571 3797 3949 6058 1493
Size: 2078 226 152 2109 1697
Fragments arranged by size:
2109 2078 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2524 3188 3368 3393 3587 1870
Size: 73 581 664 180 25 194 4545
Fragments arranged by size:
4545 664 581 194 180 73 25
BamHI G'GATC_C
Cuts at: 2051 3592 3959 2051
Size: 1541 367 4354
Fragments arranged by size:
4354 1541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3482 3583 5038 618
Size: 325 217 627 1695 101 1455 1842
Fragments arranged by size:
1842 1695 1455 627 325 217 101
BanII G_rGCy'C
Cuts at: 1391 2210 2300 2405 3170 3345 3539 3581 1391
Size: 819 90 105 765 175 194 42 4072
Fragments arranged by size:
4072 819 765 194 175 105 90 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3228 928
Size: 971 1329 3962
Fragments arranged by size:
3962 1329 971
BbvCI CC'TCA_GC
Cuts at: 2486 2820 2486
Size: 334 5928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 2550 3412 1548
Size: 1002 862 4398
Fragments arranged by size:
4398 1002 862
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2438 2462 4873
Size: 107 122 71 631 605 766 24 2411
Cuts at: 4873 136
Size: 1525
Fragments arranged by size:
2411 1525 766 631 605 122 107 71
24
BglII A'GATC_T
Cuts at: 2907 3084 6257 2907
Size: 177 3173 2912
Fragments arranged by size:
3173 2912 177
BlpI GC'TnA_GC
Cuts at: 3679 3679
Size: 6262
Bme1580I G_kGCm'C
Cuts at: 1790 2310 3180 3539 4323 5479 1790
Size: 520 870 359 784 1156 2573
Fragments arranged by size:
2573 1156 870 784 520 359
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3129 3993 4951
Size: 416 53 83 399 24 1712 864 958
Cuts at: 4951 442
Size: 1753
Fragments arranged by size:
1753 1712 958 864 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 3214 2170
Size: 1044 5218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 2671 3029 3041 3047 3073 4922 1239
Size: 1349 83 358 12 6 26 1849 2579
Fragments arranged by size:
2579 1849 1349 358 83 26 12 6
Bpu10I CC'TnA_GC
Cuts at: 2115 2273 2486 2820 2115
Size: 158 213 334 5557
Fragments arranged by size:
5557 334 213 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4387 5252 5550 4387
Size: 865 298 5099
Fragments arranged by size:
5099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3008 3428 4925 882
Size: 617 1509 420 1497 2219
Fragments arranged by size:
2219 1509 1497 617 420
BsaAI yAC'GTr
Cuts at: 493 1204 4053 493
Size: 711 2849 2702
Fragments arranged by size:
2849 2702 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2624 3243 4069
Size: 53 83 186 936 477 614 619 826
Cuts at: 4069 4451 5086 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 826 635 619 614 477 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2596 2886 4695 5093 5367 5514 5925 2596
Size: 290 1809 398 274 147 411 2933
Fragments arranged by size:
2933 1809 411 398 290 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2252 2282 3071 3101 1178
Size: 30 165 30 849 30 789 30 4339
Fragments arranged by size:
4339 849 789 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2704 2732 2810 2813 2879 3187 1186
Size: 1518 28 78 3 66 308 4261
Fragments arranged by size:
4261 1518 308 78 66 28 3
BseYI C'CCAG_C
Cuts at: 2359 2479 2630 3163 3354 5465 5961 2359
Size: 120 151 533 191 2111 496 2660
Fragments arranged by size:
2660 2111 533 496 191 151 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2630 1163
Size: 573 894 4795
Fragments arranged by size:
4795 894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2457 3712 4473 5501 1540
Size: 275 186 33 423 1255 761 1028 2301
Fragments arranged by size:
2301 1255 1028 761 423 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2121 2262 2378 4323 4408 5479 2121
Size: 141 116 1945 85 1071 2904
Fragments arranged by size:
2904 1945 1071 141 116 85
BsmI GAATG_Cn'
Cuts at: 3031 3773 3866 3031
Size: 742 93 5427
Fragments arranged by size:
5427 742 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2210 2262 2300 2310 2378 2405
Size: 399 331 89 52 38 10 68 27
Cuts at: 2405 3170 3180 3345 3539 3581 4323 4408 5479
Size: 765 10 165 194 42 742 85 1071
Cuts at: 5479 1391
Size: 2174
Fragments arranged by size:
2174 1071 765 742 399 331 194 165
89 85 68 52 42 38 27 10
10
BspEI T'CCGG_A
Cuts at: 2886 5925 2886
Size: 3039 3223
BspHI T'CATG_A
Cuts at: 4151 5881 4151
Size: 1730 4532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 3411 3617 844
Size: 2290 277 206 3489
Fragments arranged by size:
3489 2290 277 206
BsrBI CCG'CTC
Cuts at: 1394 1823 2277 2673 4149 1394
Size: 429 454 396 1476 3507
Fragments arranged by size:
3507 1476 454 429 396
BsrDI GCAATG_nn'
Cuts at: 65 1248 4757 4931 65
Size: 1183 3509 174 1396
Fragments arranged by size:
3509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3376 4906 5093 1700
Size: 110 1566 1530 187 2869
Fragments arranged by size:
2869 1566 1530 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6262
BssHII G'CGCG_C
Cuts at: 1925 2708 1925
Size: 783 5479
BssSI C'ACGA_G
Cuts at: 3173 3431 4322 5334 3173
Size: 258 891 1012 4101
Fragments arranged by size:
4101 1012 891 258
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 3073 1184
Size: 1889 4373
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2317 3354 2062
Size: 255 1037 4970
Fragments arranged by size:
4970 1037 255
BstYI r'GATC_y
Cuts at: 1972 2051 2074 2907 3084 3529 3592 3959 4344
Size: 79 23 833 177 445 63 367 385
Cuts at: 4344 4361 5813 5899 5911 6257 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 833 445 385 367 346 177
86 79 63 23 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2415 2442 2451 2460 2638 2703
Size: 552 939 411 27 9 9 178 65
Cuts at: 2703 2718 3336 513
Size: 15 618 3439
Fragments arranged by size:
3439 939 618 552 411 178 65 27
15 9 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 2650 2745 2780 3778 4572 4592 2650
Size: 95 35 998 794 20 4320
Fragments arranged by size:
4320 998 794 95 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2439 2454
Size: 54 1425 50 275 18 201 408 15
Cuts at: 2454 2463 2721 3105 3231 3252 3316 3709 4598
Size: 9 258 384 126 21 64 393 889
Cuts at: 4598 6227 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 408 393 384 275 258
201 126 64 54 50 43 21 18
15 9
EagI C'GGCC_G
Cuts at: 1537 1812 2031 2454 3709 1537
Size: 275 219 423 1255 4090
Fragments arranged by size:
4090 1255 423 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2873 4192 1353
Size: 845 675 1319 3423
Fragments arranged by size:
3423 1319 845 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 4849 5223 5369 387
Size: 1064 138 681 2579 374 146 1280
Fragments arranged by size:
2579 1280 1064 681 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2588 2671 3029 3041 3047 3073 4325
Size: 393 956 83 358 12 6 26 1252
Cuts at: 4325 4922 5709 5977 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1252 956 787 597 393 358 268
83 26 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2241 2497 2792 3452 2241
Size: 256 295 660 5051
Fragments arranged by size:
5051 660 295 256
EcoO109I rG'GnC_Cy
Cuts at: 2521 2602 3036 3123 3536 2521
Size: 81 434 87 413 5247
Fragments arranged by size:
5247 434 413 87 81
EcoRI G'AATT_C
Cuts at: 3571 3949 3571
Size: 378 5884
FspI TGC'GCA
Cuts at: 2106 4768 2106
Size: 2662 3600
FspAI rTGC'GCAy
Cuts at: 2106 2106
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 2291 2360 5409 1678
Size: 613 69 3049 2531
Fragments arranged by size:
3049 2531 613 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4903 4935 4977 5009 1373
Size: 32 135 32 3331 32 42 32 2626
Fragments arranged by size:
3331 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3606 3852 669
Size: 988 281 1668 246 3079
Fragments arranged by size:
3079 1668 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 2645 5376 5560 1305
Size: 762 578 2731 184 2007
Fragments arranged by size:
2731 2007 762 578 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 10
Size: 54 1425 343 633 3807
Fragments arranged by size:
3807 1425 633 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2384 2643 4220 4579 4738 518
Size: 648 1149 69 259 1577 359 159 2042
Fragments arranged by size:
2042 1577 1149 648 359 259 159 69
MspA1I CmG'CkG
Cuts at: 1606 2453 2476 2542 3052 3388 3678 4355 5503
Size: 847 23 66 510 336 290 677 1148
Cuts at: 5503 5748 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 847 677 510 336 290 245
66 23
NaeI GCC'GGC
Cuts at: 3378 3378
Size: 6262
NcoI C'CATG_G
Cuts at: 513 1065 2004 2442 2460 2638 2703 3336 513
Size: 552 939 438 18 178 65 633 3439
Fragments arranged by size:
3439 939 633 552 438 178 65 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NgoMIV G'CCGG_C
Cuts at: 3376 3376
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 2454 3709 2454
Size: 1255 5007
NspI r_CATG'y
Cuts at: 1132 1229 3620 5165 1132
Size: 97 2391 1545 2229
Fragments arranged by size:
2391 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3070 3513 1108
Size: 1962 443 3857
Fragments arranged by size:
3857 1962 443
PfoI T'CCnGG_A
Cuts at: 2061 2061
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 4331 4363 5844 5876 2894
Size: 32 1405 32 1481 32 3280
Fragments arranged by size:
3280 1481 1405 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2602 3036 3123 2602
Size: 434 87 5741
Fragments arranged by size:
5741 434 87
PshAI GACnn'nnGTC
Cuts at: 1613 3071 1613
Size: 1458 4804
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 3535 3535
Size: 6262
PstI C_TGCA'G
Cuts at: 830 3057 3408 3614 830
Size: 2227 351 206 3478
Fragments arranged by size:
3478 2227 351 206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 2542 3052 2542
Size: 510 5752
SacII CC_GC'GG
Cuts at: 2454 2454
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SapI GCTCTTCn'nnn_
Cuts at: 2198 2198
Size: 6262
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SexAI A'CCwGG_T
Cuts at: 2604 2604
Size: 6262
SfcI C'TryA_G
Cuts at: 826 1045 3053 3404 3610 4745 5426 5617 826
Size: 219 2008 351 206 1135 681 191 1471
Fragments arranged by size:
2008 1471 1135 681 351 219 206 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2462 2462
Size: 6262
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 2526 3370 3395 3589 1872
Size: 654 844 25 194 4545
Fragments arranged by size:
4545 844 654 194 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4366 5267 5529 820
Size: 197 926 2423 901 262 1553
Fragments arranged by size:
2423 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 2873 3458 1517
Size: 1356 585 4321
Fragments arranged by size:
4321 1356 585
StyI C'CwwG_G
Cuts at: 513 1065 2004 2442 2460 2638 2703 3336 3507
Size: 552 939 438 18 178 65 633 171
Cuts at: 3507 3701 513
Size: 194 3074
Fragments arranged by size:
3074 939 633 552 438 194 178 171
65 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2345 3128 3443 4289 4306 4459 4644
Size: 1255 765 783 315 846 17 153 185
Cuts at: 4644 5089 325
Size: 445 1498
Fragments arranged by size:
1498 1255 846 783 765 445 315 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4508 6220 96
Size: 274 80 33 51 1443 2531 1712 138
Fragments arranged by size:
2531 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 6222 6254 2252
Size: 32 3938 32 2260
Fragments arranged by size:
3938 2260 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2012 3242 1316
Size: 696 1230 4336
Fragments arranged by size:
4336 1230 696
XbaI T'CTAG_A
Cuts at: 2956 3598 2956
Size: 642 5620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 2524 3368 3393 3587 1870
Size: 654 844 25 194 4545
Fragments arranged by size:
4545 844 654 194 25
XmnI GAAnn'nnTTC
Cuts at: 2579 4391 2579
Size: 1812 4450
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SanDI SfoI SrfI SwaI