Restriction map for
pFN21AB7793
(Circular) (Six-base) MAPSORT of: pFN21AB7793.seq Check: 7284 from: 1 to: 6085
pFN21AB7793 6085 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2541 3895 278
Size: 53 83 186 936 1005 1354 2468
Fragments arranged by size:
2468 1354 1005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 1937
Size: 1491 4594
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3181 4148 5532 5800 1632
Size: 1549 967 1384 268 1917
Fragments arranged by size:
1917 1549 1384 967 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 3327 4984 5961 1203
Size: 2124 1657 977 1327
Fragments arranged by size:
2124 1657 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 2542 4814 2542
Size: 2272 3813
AleI CACnn'nnGTG
Cuts at: 1166 2097 1166
Size: 931 5154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2937 2969 3281 3313 2937
Size: 32 312 32 5709
Fragments arranged by size:
5709 312 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3828 5400 3828
Size: 1572 4513
ApaLI G'TGCA_C
Cuts at: 4142 5298 4142
Size: 1156 4929
ApoI r'AATT_y
Cuts at: 1493 2884 3101 3394 3620 3772 5881 1493
Size: 1391 217 293 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 293 226 217 152
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 2158 2379 2401 3043 3410 1870
Size: 73 161 54 221 22 642 367 4545
Fragments arranged by size:
4545 642 367 221 161 73 54 22
BamHI G'GATC_C
Cuts at: 3174 3415 3782 3174
Size: 241 367 5477
Fragments arranged by size:
5477 367 241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3221 3406 4861 618
Size: 325 217 627 1434 185 1455 1842
Fragments arranged by size:
1842 1455 1434 627 325 217 185
BanII G_rGCy'C
Cuts at: 1391 2138 3404 1391
Size: 747 1266 4072
Fragments arranged by size:
4072 1266 747
BbeI G_GCGC'C
Cuts at: 3225 3225
Size: 6085
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2541 928
Size: 971 642 4472
Fragments arranged by size:
4472 971 642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3034 3068 4276 4310 1903
Size: 34 50 34 1013 34 1208 34 3678
Fragments arranged by size:
3678 1208 1013 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6085
BfrBI ATG'CAT
Cuts at: 2798 2798
Size: 6085
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2849 6080 2849
Size: 3231 2854
BlpI GC'TnA_GC
Cuts at: 3502 3502
Size: 6085
Bme1580I G_kGCm'C
Cuts at: 1790 4146 5302 1790
Size: 2356 1156 2573
Fragments arranged by size:
2573 2356 1156
BmgBI CAC'GTC
Cuts at: 1318 2269 2449 3330 1318
Size: 951 180 881 4073
Fragments arranged by size:
4073 951 881 180
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3816 4774 442
Size: 416 53 83 399 24 2399 958 1753
Fragments arranged by size:
2399 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3185 4745 1239
Size: 1946 1560 2579
Fragments arranged by size:
2579 1946 1560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4210 5075 5373 4210
Size: 865 298 4922
Fragments arranged by size:
4922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2016 4748 882
Size: 617 517 2732 2219
Fragments arranged by size:
2732 2219 617 517
BsaAI yAC'GTr
Cuts at: 493 1204 3876 493
Size: 711 2672 2702
Fragments arranged by size:
2702 2672 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2538 3222 3892 4274
Size: 53 83 186 936 1005 684 670 382
Cuts at: 4274 4909 275
Size: 635 1451
Fragments arranged by size:
1451 1005 936 684 670 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3126 4518 4916 5190 5337 5748 3126
Size: 1392 398 274 147 411 3463
Fragments arranged by size:
3463 1392 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2267 2276 2297 2937
Size: 30 165 30 843 21 9 21 640
Cuts at: 2937 2967 3041 3071 1178
Size: 30 74 30 4192
Fragments arranged by size:
4192 843 640 165 74 30 30 30
30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2795 3383 1186
Size: 1609 588 3888
Fragments arranged by size:
3888 1609 588
BseYI C'CCAG_C
Cuts at: 2127 2131 2307 2373 2394 3152 5288 5784 2127
Size: 4 176 66 21 758 2136 496 2428
Fragments arranged by size:
2428 2136 758 496 176 66 21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2492 3038 1163
Size: 573 546 210 546 4210
Fragments arranged by size:
4210 573 546 546 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2444 3535 4296 5324 1540
Size: 275 186 443 1091 761 1028 2301
Fragments arranged by size:
2301 1091 1028 761 443 275 186
BsiHKAI G_wGCw'C
Cuts at: 4146 4231 5302 4146
Size: 85 1071 4929
Fragments arranged by size:
4929 1071 85
BsmI GAATG_Cn'
Cuts at: 2458 2792 3596 3689 2458
Size: 334 804 93 4854
Fragments arranged by size:
4854 804 334 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2761 3016 2761
Size: 255 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 3404 4146 4231 5302 1391
Size: 399 348 1266 742 85 1071 2174
Fragments arranged by size:
2174 1266 1071 742 399 348 85
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 3974 5704 3974
Size: 1730 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3060 3440 844
Size: 2216 380 3489
Fragments arranged by size:
3489 2216 380
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 3972 1394
Size: 429 410 1739 3507
Fragments arranged by size:
3507 1739 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 4580 4754 65
Size: 1183 3332 174 1396
Fragments arranged by size:
3332 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2357 2439 2681 3027 4729 4916
Size: 110 278 269 82 242 346 1702 187
Cuts at: 4916 1700
Size: 2869
Fragments arranged by size:
2869 1702 346 278 269 242 187 110
82
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 4145 5157 4145
Size: 1012 5073
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 2735 4889 2735
Size: 2154 3931
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 2849 3109 3174 3415 3782 4167 4184 5636
Size: 877 260 65 241 367 385 17 1452
Cuts at: 5636 5722 5734 6080 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 877 385 367 346 260 241
86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2470 2892 513
Size: 552 1405 422 3706
Fragments arranged by size:
3706 1405 552 422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 2294 3601 4395 4415 2294
Size: 1307 794 20 3964
Fragments arranged by size:
3964 1307 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2591 5092 809
Size: 507 1275 2501 1802
Fragments arranged by size:
2501 1802 1275 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2441 2518 3078
Size: 54 1425 50 275 18 611 77 560
Cuts at: 3078 3228 3532 4421 6050 8
Size: 150 304 889 1629 43
Fragments arranged by size:
1629 1425 889 611 560 304 275 150
77 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2441 3532 1537
Size: 275 629 1091 4090
Fragments arranged by size:
4090 1091 629 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2818 4015 1353
Size: 1465 1197 3423
Fragments arranged by size:
3423 1465 1197
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2426 2826 4672 5046 5192 387
Size: 1064 138 837 400 1846 374 146 1280
Fragments arranged by size:
1846 1280 1064 837 400 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3181 3185 4148 4745 5532 5800 1239
Size: 393 1549 4 963 597 787 268 1524
Fragments arranged by size:
1549 1524 963 787 597 393 268 4
EcoO109I rG'GnC_Cy
Cuts at: 2150 2254 2150
Size: 104 5981
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
EcoRV GAT'ATC
Cuts at: 2077 2077
Size: 6085
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3316 3316
Size: 6085
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 2166 3225 3278 5232 1678
Size: 488 1059 53 1954 2531
Fragments arranged by size:
2531 1954 1059 488 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2267 2278 2299 2414
Size: 32 135 32 674 21 11 21 115
Cuts at: 2414 2446 2486 2518 2528 2560 3041 3073 4726
Size: 32 40 32 10 32 481 32 1653
Cuts at: 4726 4758 4800 4832 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1653 674 481 135 115 42 40
32 32 32 32 32 32 32 32
21 21 11 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 2677 3429 3675 669
Size: 988 281 334 405 752 246 3079
Fragments arranged by size:
3079 988 752 405 334 281 246
HindIII A'AGCT_T
Cuts at: 748 2048 748
Size: 1300 4785
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KasI G'GCGC_C
Cuts at: 3221 3221
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2172 5199 5383 1305
Size: 867 3027 184 2007
Fragments arranged by size:
3027 2007 867 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3080 10
Size: 54 1425 343 1248 3015
Fragments arranged by size:
3015 1425 1248 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2097 4043 4402 4561 518
Size: 648 931 1946 359 159 2042
Fragments arranged by size:
2042 1946 931 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2131 2173 2335 2568 2894 3501 4178 5326
Size: 525 42 162 233 326 607 677 1148
Cuts at: 5326 5571 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 607 525 326 245 233
162 42
NaeI GCC'GGC
Cuts at: 2359 2441 2359
Size: 82 6003
NarI GG'CG_CC
Cuts at: 3222 3222
Size: 6085
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5533
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NgoMIV G'CCGG_C
Cuts at: 2357 2439 2357
Size: 82 6003
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NsiI A_TGCA'T
Cuts at: 2800 2800
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 2671 3443 4988 1132
Size: 97 1442 772 1545 2229
Fragments arranged by size:
2229 1545 1442 772 97
PasI CC'CwG_GG
Cuts at: 1348 2319 1348
Size: 971 5114
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2476 1108
Size: 1368 4717
PfoI T'CCnGG_A
Cuts at: 2524 3162 2524
Size: 638 5447
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2937 2969 4154 4186 5667 5699 2937
Size: 32 1185 32 1481 32 3323
Fragments arranged by size:
3323 1481 1185 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2150 2254 2150
Size: 104 5981
PshAI GACnn'nnGTC
Cuts at: 1613 2773 1613
Size: 1160 4925
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspXI vC'TCGA_Gb
Cuts at: 2158 2158
Size: 6085
PstI C_TGCA'G
Cuts at: 830 3258 3437 830
Size: 2428 179 3478
Fragments arranged by size:
3478 2428 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2131 2131
Size: 6085
RsrII CG'GwC_CG
Cuts at: 2434 2434
Size: 6085
SacII CC_GC'GG
Cuts at: 2895 2895
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SapI GCTCTTCn'nnn_
Cuts at: 2818 2818
Size: 6085
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 3254 3433 4568 5249 5440 826
Size: 219 2209 179 1135 681 191 1471
Fragments arranged by size:
2209 1471 1135 681 219 191 179
SfoI GGC'GCC
Cuts at: 3223 3223
Size: 6085
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 2681 1700
Size: 981 5104
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2158 4189 5090 5352 820
Size: 197 926 215 2031 901 262 1553
Fragments arranged by size:
2031 1553 926 901 262 215 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6085
StyI C'CwwG_G
Cuts at: 513 1065 2146 3524 513
Size: 552 1081 1378 3074
Fragments arranged by size:
3074 1378 1081 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 2480 4112 4129 4282 4467 4912
Size: 1255 842 58 1632 17 153 185 445
Cuts at: 4912 325
Size: 1498
Fragments arranged by size:
1632 1498 1255 842 445 185 153 58
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 4331 6043
Size: 274 80 33 51 1443 762 1592 1712
Cuts at: 6043 96
Size: 138
Fragments arranged by size:
1712 1592 1443 762 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6045 6077 6045
Size: 32 6053
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6085
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 3160
Size: 6085
XhoI C'TCGA_G
Cuts at: 1943 2158 1943
Size: 215 5870
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 2952 4214 2952
Size: 1262 4823
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2539 3893 276
Size: 53 83 186 936 1005 1354 2468
Fragments arranged by size:
2468 1354 1005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII RsrII SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI NruI PacI PmlI PspOMI PsrI SacI
SanDI SexAI SfiI SrfI SwaI