Restriction map for
pFN21AB7791
(Circular) (Six-base) MAPSORT of: pFN21AB7791.seq Check: 8430 from: 1 to: 6082
pFN21AB7791 6082 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2588 2588
Size: 6082
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3425 1937
Size: 1488 4594
Acc65I G'GTAC_C
Cuts at: 3403 3403
Size: 6082
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 3133 4145 5529 5797 1632
Size: 544 957 1012 1384 268 1917
Fragments arranged by size:
1917 1384 1012 957 544 268
AfeI AGC'GCT
Cuts at: 2144 2978 2144
Size: 834 5248
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 4981 5958 1203
Size: 3778 977 1327
Fragments arranged by size:
3778 1327 977
AgeI A'CCGG_T
Cuts at: 3275 4913 3275
Size: 1638 4444
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 3040 3276 1166
Size: 1874 236 3972
Fragments arranged by size:
3972 1874 236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 2257 2284 3219 3352 3825 5397 2257
Size: 27 935 133 473 1572 2942
Fragments arranged by size:
2942 1572 935 473 133 27
ApaI G_GGCC'C
Cuts at: 2879 2879
Size: 6082
ApaLI G'TGCA_C
Cuts at: 3101 3269 4139 5295 3101
Size: 168 870 1156 3888
Fragments arranged by size:
3888 1156 870 168
ApoI r'AATT_y
Cuts at: 1493 3391 3617 3769 5878 1493
Size: 1898 226 152 2109 1697
Fragments arranged by size:
2109 1898 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 2332 2429 2640 2825 3407 1870
Size: 73 288 101 97 211 185 582 4545
Fragments arranged by size:
4545 582 288 211 185 101 97 73
BamHI G'GATC_C
Cuts at: 2447 3412 3779 2447
Size: 965 367 4750
Fragments arranged by size:
4750 965 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2654 2773 2782 2919 3006
Size: 325 217 627 867 119 9 137 87
Cuts at: 3006 3403 4858 618
Size: 397 1455 1842
Fragments arranged by size:
1842 1455 867 627 397 325 217 137
119 87 9
BanII G_rGCy'C
Cuts at: 1391 2211 2736 2825 2879 2993 3401 1391
Size: 820 525 89 54 114 408 4072
Fragments arranged by size:
4072 820 525 408 114 89 54
BbeI G_GCGC'C
Cuts at: 2923 2923
Size: 6082
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5111
BbvCI CC'TCA_GC
Cuts at: 2835 2835
Size: 6082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5184 3974
Size: 1210 4872
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2796 3096 3167
Size: 107 122 71 631 605 1124 300 71
Cuts at: 3167 3365 4693 136
Size: 198 1328 1525
Fragments arranged by size:
1525 1328 1124 631 605 300 198 122
107 71 71
BglII A'GATC_T
Cuts at: 6077 6077
Size: 6082
BlpI GC'TnA_GC
Cuts at: 2670 3499 2670
Size: 829 5253
Bme1580I G_kGCm'C
Cuts at: 1790 2412 2485 2657 2879 3047 3105 3273 4143
Size: 622 73 172 222 168 58 168 870
Cuts at: 4143 5299 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 870 622 222 172 168 168
73 58
BmgBI CAC'GTC
Cuts at: 1318 2806 1318
Size: 1488 4594
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2873 3813 4771
Size: 416 53 83 399 24 1456 940 958
Cuts at: 4771 442
Size: 1753
Fragments arranged by size:
1753 1456 958 940 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2294 2839 4742 1239
Size: 1040 15 545 1903 2579
Fragments arranged by size:
2579 1903 1040 545 15
Bpu10I CC'TnA_GC
Cuts at: 2835 2835
Size: 6082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4207 5072 5370 4207
Size: 865 298 4919
Fragments arranged by size:
4919 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4745 882
Size: 617 3246 2219
Fragments arranged by size:
3246 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3873 493
Size: 711 2669 2702
Fragments arranged by size:
2702 2669 711
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2920 3889 4271 4906
Size: 53 83 186 936 1387 969 382 635
Cuts at: 4906 275
Size: 1451
Fragments arranged by size:
1451 1387 969 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3275 4515 4913 5187 5334 5745 3275
Size: 1240 398 274 147 411 3612
Fragments arranged by size:
3612 1240 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2449 2479 1178
Size: 30 165 30 1046 30 4781
Fragments arranged by size:
4781 1046 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2360 2432 2843 3064 1186
Size: 1174 72 411 221 4204
Fragments arranged by size:
4204 1174 411 221 72
BseYI C'CCAG_C
Cuts at: 2103 2313 2529 2985 3369 5285 5781 2103
Size: 210 216 456 384 1916 496 2404
Fragments arranged by size:
2404 1916 496 456 384 216 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3338 1163
Size: 573 1602 3907
Fragments arranged by size:
3907 1602 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2495 2861 3532 4293 5321 1540
Size: 275 186 494 366 671 761 1028 2301
Fragments arranged by size:
2301 1028 761 671 494 366 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 3105 3273 4143 4228 5299 2211
Size: 894 168 870 85 1071 2994
Fragments arranged by size:
2994 1071 894 870 168 85
BsmI GAATG_Cn'
Cuts at: 3593 3686 3593
Size: 93 5989
BsmBI CGTCTCn'nnnn_
Cuts at: 2561 2561
Size: 6082
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2412 2485 2657 2736 2825 2879
Size: 399 421 201 73 172 79 89 54
Cuts at: 2879 2993 3047 3105 3273 3401 4143 4228 5299
Size: 114 54 58 168 128 742 85 1071
Cuts at: 5299 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 421 399 201 172 168
128 114 89 85 79 73 58 54
54
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 2182 3971 5701 2182
Size: 1789 1730 2563
Fragments arranged by size:
2563 1789 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2588 3258 3437 844
Size: 1744 670 179 3489
Fragments arranged by size:
3489 1744 670 179
BsrBI CCG'CTC
Cuts at: 1394 1823 3283 3969 1394
Size: 429 1460 686 3507
Fragments arranged by size:
3507 1460 686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4577 4751 65
Size: 1183 3329 174 1396
Fragments arranged by size:
3329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2776 2856 2916 3275 4726 4913 1700
Size: 110 966 80 60 359 1451 187 2869
Fragments arranged by size:
2869 1451 966 359 187 110 80 60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6082
BssHII G'CGCG_C
Cuts at: 1925 2889 3325 3327 1925
Size: 964 436 2 4680
Fragments arranged by size:
4680 964 436 2
BssSI C'ACGA_G
Cuts at: 4142 5154 4142
Size: 1012 5070
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3219 794
Size: 2425 3657
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstXI CCAn_nnnn'nTGG
Cuts at: 2039 2039
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 2253 2304 2447 2677 3412 3779 4164 4181
Size: 281 51 143 230 735 367 385 17
Cuts at: 4181 5633 5719 5731 6077 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 735 385 367 346 281 230
143 86 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 2599 3375 513
Size: 552 939 453 142 776 3220
Fragments arranged by size:
3220 939 776 552 453 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3322 487
Size: 683 56 2096 3247
Fragments arranged by size:
3247 2096 683 56
BtsI GCAGTG_nn'
Cuts at: 2972 3598 4392 4412 2972
Size: 626 794 20 4642
Fragments arranged by size:
4642 794 626 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2492 2858 2903
Size: 54 1425 50 275 18 662 366 45
Cuts at: 2903 3168 3529 4418 6047 8
Size: 265 361 889 1629 43
Fragments arranged by size:
1629 1425 889 662 366 361 275 265
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2492 2858 3529 1537
Size: 275 680 366 671 4090
Fragments arranged by size:
4090 680 671 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2547 2687 4012 1353
Size: 1194 140 1325 3423
Fragments arranged by size:
3423 1325 1194 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2438 4669 5043 5189 387
Size: 1064 138 849 2231 374 146 1280
Fragments arranged by size:
2231 1280 1064 849 374 146 138
EcoICRI GAG'CTC
Cuts at: 2209 2209
Size: 6082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2279 2294 2839 3133 4145 4742
Size: 393 544 103 15 545 294 1012 597
Cuts at: 4742 5529 5797 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1012 787 597 545 544 393 294
268 103 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2762 2762
Size: 6082
EcoO109I rG'GnC_Cy
Cuts at: 2035 2368 2462 2932 2035
Size: 333 94 470 5185
Fragments arranged by size:
5185 470 333 94
EcoRI G'AATT_C
Cuts at: 3391 3769 3391
Size: 378 5704
FspI TGC'GCA
Cuts at: 4588 4588
Size: 6082
HaeII r_GCGC'y
Cuts at: 1678 2146 2529 2923 2980 3162 5229 1678
Size: 468 383 394 57 182 2067 2531
Fragments arranged by size:
2531 2067 468 394 383 182 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2396 2428 4723 4755 4797
Size: 32 135 32 824 32 2295 32 42
Cuts at: 4797 4829 1373
Size: 32 2626
Fragments arranged by size:
2626 2295 824 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3426 3672 669
Size: 988 281 1488 246 3079
Fragments arranged by size:
3079 1488 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6082
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KasI G'GCGC_C
Cuts at: 2919 2919
Size: 6082
KpnI G_GTAC'C
Cuts at: 3407 3407
Size: 6082
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4260
Fragments arranged by size:
4260 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 3040 3264 3276 4040 4399 4558
Size: 648 946 928 224 12 764 359 159
Cuts at: 4558 518
Size: 2042
Fragments arranged by size:
2042 946 928 764 648 359 224 159
12
MspA1I CmG'CkG
Cuts at: 1606 2220 2674 2958 2985 3498 4175 5323 5568
Size: 614 454 284 27 513 677 1148 245
Cuts at: 5568 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 614 513 454 284 245
27
NaeI GCC'GGC
Cuts at: 2918 2918
Size: 6082
NarI GG'CG_CC
Cuts at: 2920 2920
Size: 6082
NcoI C'CATG_G
Cuts at: 513 1065 2004 2599 513
Size: 552 939 595 3996
Fragments arranged by size:
3996 939 595 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6082
NgoMIV G'CCGG_C
Cuts at: 2916 2916
Size: 6082
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 3440 4985 1132
Size: 97 2211 1545 2229
Fragments arranged by size:
2229 2211 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2039 2722 2872 1348
Size: 691 683 150 4558
Fragments arranged by size:
4558 691 683 150
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3376 1108
Size: 2268 3814
PfoI T'CCnGG_A
Cuts at: 2256 2560 2708 2816 2928 2256
Size: 304 148 108 112 5410
Fragments arranged by size:
5410 304 148 112 108
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2368 2932 2368
Size: 564 5518
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6082
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PspOMI G'GGCC_C
Cuts at: 2875 2875
Size: 6082
PstI C_TGCA'G
Cuts at: 830 2440 2766 3215 3434 830
Size: 1610 326 449 219 3478
Fragments arranged by size:
3478 1610 449 326 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
PvuII CAG'CTG
Cuts at: 2958 2958
Size: 6082
SacI G_AGCT'C
Cuts at: 2211 2211
Size: 6082
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SapI GCTCTTCn'nnn_
Cuts at: 2547 2547
Size: 6082
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SexAI A'CCwGG_T
Cuts at: 3081 3081
Size: 6082
SfcI C'TryA_G
Cuts at: 826 1045 2372 2436 2762 3211 3430 4565 5246
Size: 219 1327 64 326 449 219 1135 681
Cuts at: 5246 5437 826
Size: 191 1471
Fragments arranged by size:
1471 1327 1135 681 449 326 219 219
191 64
SfoI GGC'GCC
Cuts at: 2921 2921
Size: 6082
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 2916 3275 1700
Size: 1216 359 4507
Fragments arranged by size:
4507 1216 359
SmaI CCC'GGG
Cuts at: 1872 2334 2642 3409 1872
Size: 462 308 767 4545
Fragments arranged by size:
4545 767 462 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4186 5087 5349 820
Size: 197 926 2243 901 262 1553
Fragments arranged by size:
2243 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 2189 2840 1517
Size: 672 651 4759
Fragments arranged by size:
4759 672 651
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2244 2358 2599 3094 3521
Size: 552 939 12 228 114 241 495 427
Cuts at: 3521 513
Size: 3074
Fragments arranged by size:
3074 939 552 495 427 241 228 114
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2305 2923 4109 4126 4279 4464 4909
Size: 1255 725 618 1186 17 153 185 445
Cuts at: 4909 325
Size: 1498
Fragments arranged by size:
1498 1255 1186 725 618 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4328 6040 96
Size: 274 80 33 51 1443 2351 1712 138
Fragments arranged by size:
2351 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2449 2481 6042 6074 2449
Size: 32 3561 32 2457
Fragments arranged by size:
3561 2457 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2812 1316
Size: 1496 4586
XbaI T'CTAG_A
Cuts at: 3418 3418
Size: 6082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2111 2391 2111
Size: 280 5802
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 2332 2640 3407 1870
Size: 462 308 767 4545
Fragments arranged by size:
4545 767 462 308
XmnI GAAnn'nnTTC
Cuts at: 2055 3119 3203 4211 2055
Size: 1064 84 1008 3926
Fragments arranged by size:
3926 1064 1008 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI
SrfI SwaI