Restriction map for
pFN21AB7789
(Circular) (Six-base) MAPSORT of: pFN21AB7789.seq Check: 7100 from: 1 to: 6079
pFN21AB7789 6079 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2316 3422 1937
Size: 379 1106 4594
Fragments arranged by size:
4594 1106 379
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2031 2244 4142 5526 5794 1632
Size: 399 213 1898 1384 268 1917
Fragments arranged by size:
1917 1898 1384 399 268 213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 4978 5955 1203
Size: 3775 977 1327
Fragments arranged by size:
3775 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 2528 1166
Size: 1362 4717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 2791 3096 3822 5394 2791
Size: 305 726 1572 3476
Fragments arranged by size:
3476 1572 726 305
ApaI G_GGCC'C
Cuts at: 2079 2893 2079
Size: 814 5265
ApaLI G'TGCA_C
Cuts at: 4136 5292 4136
Size: 1156 4923
ApoI r'AATT_y
Cuts at: 1493 2267 3388 3614 3766 5875 1493
Size: 774 1121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 774 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2078 2116 2238 2711 3404 1870
Size: 73 129 6 38 122 473 693 4545
Fragments arranged by size:
4545 693 473 129 122 73 38 6
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3340 3400 4855 618
Size: 325 217 627 1553 60 1455 1842
Fragments arranged by size:
1842 1553 1455 627 325 217 60
BanII G_rGCy'C
Cuts at: 1391 2073 2079 2394 2893 3021 3398 1391
Size: 682 6 315 499 128 377 4072
Fragments arranged by size:
4072 682 499 377 315 128 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2471 2607 928
Size: 971 572 136 4400
Fragments arranged by size:
4400 971 572 136
BbvCI CC'TCA_GC
Cuts at: 2097 3158 2097
Size: 1061 5018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2555 2589 4270 4304 1903
Size: 34 618 34 1681 34 3678
Fragments arranged by size:
3678 1681 618 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4690 136
Size: 107 122 71 631 605 337 2681 1525
Fragments arranged by size:
2681 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2721 6074 2721
Size: 3353 2726
BlpI GC'TnA_GC
Cuts at: 3080 3496 3080
Size: 416 5663
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2893 3140 3292 3343 4140 5296 1790
Size: 289 814 247 152 51 797 1156 2573
Fragments arranged by size:
2573 1156 814 797 289 247 152 51
BmgBI CAC'GTC
Cuts at: 1318 2842 1318
Size: 1524 4555
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3286 3810 4768
Size: 416 53 83 399 24 1869 524 958
Cuts at: 4768 442
Size: 1753
Fragments arranged by size:
1869 1753 958 524 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3050 3134 4739 1239
Size: 1811 84 1605 2579
Fragments arranged by size:
2579 1811 1605 84
Bpu10I CC'TnA_GC
Cuts at: 2097 2384 2406 3158 2097
Size: 287 22 752 5018
Fragments arranged by size:
5018 752 287 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2630 3317 4204 5069 5367 2630
Size: 687 887 865 298 3342
Fragments arranged by size:
3342 887 865 687 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4742 882
Size: 617 3243 2219
Fragments arranged by size:
3243 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2284 3244 3870 493
Size: 711 1080 960 626 2702
Fragments arranged by size:
2702 1080 960 711 626
BsaBI GATnn'nnATC
Cuts at: 2552 6073 2552
Size: 3521 2558
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2200 4512 4910 5184 5331 5742 2200
Size: 2312 398 274 147 411 2537
Fragments arranged by size:
2537 2312 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2909 2939 1178
Size: 30 165 30 1506 30 4318
Fragments arranged by size:
4318 1506 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3287 1186
Size: 2101 3978
BseYI C'CCAG_C
Cuts at: 5282 5778 5282
Size: 496 5583
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 1163
Size: 573 744 4762
Fragments arranged by size:
4762 744 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2201 3529 4290 5318 1540
Size: 275 186 93 107 1328 761 1028 2301
Fragments arranged by size:
2301 1328 1028 761 275 186 107 93
BsiHKAI G_wGCw'C
Cuts at: 2394 3021 3038 3279 4140 4225 5296 2394
Size: 627 17 241 861 85 1071 3177
Fragments arranged by size:
3177 1071 861 627 241 85 17
BsmI GAATG_Cn'
Cuts at: 2092 2215 3590 3683 2092
Size: 123 1375 93 4488
Fragments arranged by size:
4488 1375 123 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2131 2131
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2079 2394 2893 3021 3038 3140
Size: 399 283 6 315 499 128 17 102
Cuts at: 3140 3279 3292 3343 3398 4140 4225 5296 1391
Size: 139 13 51 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 499 399 315 283 139
128 102 85 55 51 17 13 6
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3434 844
Size: 2590 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2193 2295 2325 2880 4723 4910 1700
Size: 110 383 102 30 555 1843 187 2869
Fragments arranged by size:
2869 1843 555 383 187 110 102 30
BsrGI T'GTAC_A
Cuts at: 96 2275 2359 96
Size: 2179 84 3816
Fragments arranged by size:
3816 2179 84
BssHII G'CGCG_C
Cuts at: 1925 2144 1925
Size: 219 5860
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstXI CCAn_nnnn'nTGG
Cuts at: 2900 3268 3316 2900
Size: 368 48 5663
Fragments arranged by size:
5663 368 48
BstYI r'GATC_y
Cuts at: 1972 2547 2721 3117 3409 3776 4161 4178 5630
Size: 575 174 396 292 367 385 17 1452
Cuts at: 5630 5716 5728 6074 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 575 396 385 367 346 292
174 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2980 513
Size: 552 939 976 3612
Fragments arranged by size:
3612 976 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2986 487
Size: 683 56 1760 3580
Fragments arranged by size:
3580 1760 683 56
BtsI GCAGTG_nn'
Cuts at: 3595 4389 4409 3595
Size: 794 20 5265
Fragments arranged by size:
5265 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DraIII CAC_nnn'GTG
Cuts at: 2284 2284
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2802 3526
Size: 54 1425 50 275 18 261 711 724
Cuts at: 3526 4415 6044 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 724 711 275 261 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2091 3526 1537
Size: 275 279 1435 4090
Fragments arranged by size:
4090 1435 279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2660 4009 1353
Size: 1307 1349 3423
Fragments arranged by size:
3423 1349 1307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2121 4666 5040 5186 387
Size: 1064 138 532 2545 374 146 1280
Fragments arranged by size:
2545 1280 1064 532 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 3019 2392
Size: 627 5452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2244 3050 3134 4142 4739 5526
Size: 393 399 213 806 84 1008 597 787
Cuts at: 5526 5794 1239
Size: 268 1524
Fragments arranged by size:
1524 1008 806 787 597 399 393 268
213 84
EcoNI CCTnn'n_nnAGG
Cuts at: 2870 2870
Size: 6079
EcoO109I rG'GnC_Cy
Cuts at: 2889 3154 2889
Size: 265 5814
EcoRI G'AATT_C
Cuts at: 2267 3388 3766 2267
Size: 1121 378 4580
Fragments arranged by size:
4580 1121 378
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 2336 5226 1678
Size: 658 2890 2531
Fragments arranged by size:
2890 2531 658
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2221 2253 2767 2799 2909
Size: 32 135 32 649 32 514 32 110
Cuts at: 2909 2941 4720 4752 4794 4826 1373
Size: 32 1779 32 42 32 2626
Fragments arranged by size:
2626 1779 649 514 135 110 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3423 3669 669
Size: 988 281 1485 246 3079
Fragments arranged by size:
3079 1485 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2807 748
Size: 2059 4020
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5193 5377 1305
Size: 3888 184 2007
Fragments arranged by size:
3888 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2804 10
Size: 54 1425 343 972 3285
Fragments arranged by size:
3285 1425 972 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2744 3314 4037 4396 4555 518
Size: 648 1362 216 570 723 359 159 2042
Fragments arranged by size:
2042 1362 723 648 570 359 216 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2982 3061 3495 4172 5320 5565 1606
Size: 490 886 79 434 677 1148 245 2120
Fragments arranged by size:
2120 1148 886 677 490 434 245 79
NaeI GCC'GGC
Cuts at: 2195 2195
Size: 6079
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NgoMIV G'CCGG_C
Cuts at: 2193 2193
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 2091 3526 2091
Size: 1435 4644
NspI r_CATG'y
Cuts at: 1132 1229 3437 4982 1132
Size: 97 2208 1545 2229
Fragments arranged by size:
2229 2208 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2625 1108
Size: 1517 4562
PfoI T'CCnGG_A
Cuts at: 2814 2814
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PmlI CAC'GTG
Cuts at: 3244 3244
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3154 3154
Size: 6079
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 2075 2889 2075
Size: 814 5265
PstI C_TGCA'G
Cuts at: 830 3431 830
Size: 2601 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
SacI G_AGCT'C
Cuts at: 2394 3021 2394
Size: 627 5452
SacII CC_GC'GG
Cuts at: 2983 2983
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SapI GCTCTTCn'nnn_
Cuts at: 2660 2660
Size: 6079
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 2639 3227 4327 6039 2639
Size: 588 1100 1712 2679
Fragments arranged by size:
2679 1712 1100 588
SfcI C'TryA_G
Cuts at: 826 1045 3427 4562 5243 5434 826
Size: 219 2382 1135 681 191 1471
Fragments arranged by size:
2382 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 2074 2080 2118 2713 3406 1872
Size: 202 6 38 595 693 4545
Fragments arranged by size:
4545 693 595 202 38 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2238 2645 3332 4183 5084 5346
Size: 197 926 295 407 687 851 901 262
Cuts at: 5346 820
Size: 1553
Fragments arranged by size:
1553 926 901 851 687 407 295 262
197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SrfI GCCC'GGGC
Cuts at: 2074 2074
Size: 6079
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 2526 2913 3518 513
Size: 552 939 495 27 387 605 3074
Fragments arranged by size:
3074 939 605 552 495 387 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3094 3194 3274 3322 4106 4123 4276
Size: 1255 1514 100 80 48 784 17 153
Cuts at: 4276 4461 4906 325
Size: 185 445 1498
Fragments arranged by size:
1514 1498 1255 784 445 185 153 100
80 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2275 2359 2637
Size: 274 80 33 51 1443 298 84 278
Cuts at: 2637 2649 2832 3225 4325 6037 96
Size: 12 183 393 1100 1712 138
Fragments arranged by size:
1712 1443 1100 393 298 278 274 183
138 84 80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2928 2960 6039 6071 2928
Size: 32 3079 32 2936
Fragments arranged by size:
3079 2936 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3323 1316
Size: 2007 4072
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2812 2497
Size: 315 5764
XhoI C'TCGA_G
Cuts at: 1943 2238 1943
Size: 295 5784
XmaI C'CCGG_G
Cuts at: 1870 2072 2078 2116 2711 3404 1870
Size: 202 6 38 595 693 4545
Fragments arranged by size:
4545 693 595 202 38 6
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SfoI SwaI