Restriction map for
pFN21AB7787
(Circular) (Six-base) MAPSORT of: pFN21AB7787.seq Check: 2869 from: 1 to: 6079
pFN21AB7787 6079 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3306 3422 1937
Size: 1369 116 4594
Fragments arranged by size:
4594 1369 116
Acc65I G'GTAC_C
Cuts at: 2166 3400 2166
Size: 1234 4845
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2349 2384 2497 3085 4142 5526 5794 1632
Size: 717 35 113 588 1057 1384 268 1917
Fragments arranged by size:
1917 1384 1057 717 588 268 113 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 2399 4978 5955 1203
Size: 1196 2579 977 1327
Fragments arranged by size:
2579 1327 1196 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 2302 4808 2302
Size: 2506 3573
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 3822 5394 3822
Size: 1572 4507
ApaI G_GGCC'C
Cuts at: 2116 2116
Size: 6079
ApaLI G'TGCA_C
Cuts at: 2066 4136 5292 2066
Size: 2070 1156 2853
Fragments arranged by size:
2853 2070 1156
ApoI r'AATT_y
Cuts at: 1493 2140 2322 3051 3135 3356 3388 3614 3766
Size: 647 182 729 84 221 32 226 152
Cuts at: 3766 5875 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 729 647 226 221 182 152
84 32
AseI AT'TA_AT
Cuts at: 160 1032 2312 4633 160
Size: 872 1280 2321 1606
Fragments arranged by size:
2321 1606 1280 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 3404 1870
Size: 73 1461 4545
Fragments arranged by size:
4545 1461 73
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2622 3042 3400 4855
Size: 325 217 627 379 456 420 358 1455
Cuts at: 4855 618
Size: 1842
Fragments arranged by size:
1842 1455 627 456 420 379 358 325
217
BanII G_rGCy'C
Cuts at: 1391 2116 3398 1391
Size: 725 1282 4072
Fragments arranged by size:
4072 1282 725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3144 928
Size: 971 1245 3863
Fragments arranged by size:
3863 1245 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 2700 1548
Size: 1152 4927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 4690 136
Size: 107 122 71 631 605 439 2579 1525
Fragments arranged by size:
2579 1525 631 605 439 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 2070 2116 4140 5296 1790
Size: 280 46 2024 1156 2573
Fragments arranged by size:
2573 2024 1156 280 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6079
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2165 2565 2817
Size: 416 53 83 399 24 748 400 252
Cuts at: 2817 3385 3810 4768 442
Size: 568 425 958 1753
Fragments arranged by size:
1753 958 748 568 425 416 400 399
252 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2391 2391
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2661 2913 3249 3333 4739 1239
Size: 1422 252 336 84 1406 2579
Fragments arranged by size:
2579 1422 1406 336 252 84
Bpu10I CC'TnA_GC
Cuts at: 3108 3108
Size: 6079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3317 4204 5069 5367 3317
Size: 887 865 298 4029
Fragments arranged by size:
4029 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 2641 4742 882
Size: 617 780 362 2101 2219
Fragments arranged by size:
2219 2101 780 617 362
BsaAI yAC'GTr
Cuts at: 493 1204 2402 3870 493
Size: 711 1198 1468 2702
Fragments arranged by size:
2702 1468 1198 711
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4512 4910 5184 5331 5742 4512
Size: 398 274 147 411 4849
Fragments arranged by size:
4849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5854
Fragments arranged by size:
5854 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2678 3077 1186
Size: 1324 168 399 4188
Fragments arranged by size:
4188 1324 399 168
BseYI C'CCAG_C
Cuts at: 2248 3282 5282 5778 2248
Size: 1034 2000 496 2549
Fragments arranged by size:
2549 2000 1034 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2169 1163
Size: 573 433 5073
Fragments arranged by size:
5073 573 433
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3529 4290 5318 1540
Size: 275 186 1528 761 1028 2301
Fragments arranged by size:
2301 1528 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 3109 4140 4225 5296 2070
Size: 1039 1031 85 1071 2853
Fragments arranged by size:
2853 1071 1039 1031 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2116 3109 3398 4140 4225 5296
Size: 399 280 46 993 289 742 85 1071
Cuts at: 5296 1391
Size: 2174
Fragments arranged by size:
2174 1071 993 742 399 289 280 85
46
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2191 3434 844
Size: 1347 1243 3489
Fragments arranged by size:
3489 1347 1243
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4723 4910 1700
Size: 110 2913 187 2869
Fragments arranged by size:
2913 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2744 96
Size: 2648 3431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 2044 3290 1184
Size: 860 1246 3973
Fragments arranged by size:
3973 1246 860
BstYI r'GATC_y
Cuts at: 1972 3409 3776 4161 4178 5630 5716 5728 6074
Size: 1437 367 385 17 1452 86 12 346
Cuts at: 6074 1972
Size: 1977
Fragments arranged by size:
1977 1452 1437 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 3595 4389 4409 3595
Size: 794 20 5265
Fragments arranged by size:
5265 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3526 4415 6044
Size: 54 1425 50 275 18 1696 889 1629
Cuts at: 6044 8
Size: 43
Fragments arranged by size:
1696 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 4009 1353
Size: 1007 1649 3423
Fragments arranged by size:
3423 1649 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2384 2497 2661 2913 3085 3249
Size: 393 717 35 113 164 252 172 164
Cuts at: 3249 3333 4142 4739 5526 5794 1239
Size: 84 809 597 787 268 1524
Fragments arranged by size:
1524 809 787 717 597 393 268 252
172 164 164 113 84 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2473 2473
Size: 6079
EcoO109I rG'GnC_Cy
Cuts at: 2112 2113 2224 2112
Size: 1 111 5967
Fragments arranged by size:
5967 111 1
EcoRI G'AATT_C
Cuts at: 3051 3135 3388 3766 3051
Size: 84 253 378 5364
Fragments arranged by size:
5364 378 253 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2499 2499
Size: 6079
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2638 2670 2692
Size: 32 135 32 716 32 318 32 22
Cuts at: 2692 2724 2932 2964 4720 4752 4794 4826 1373
Size: 32 208 32 1756 32 42 32 2626
Fragments arranged by size:
2626 1756 716 318 208 135 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3423 3669 669
Size: 988 281 1485 246 3079
Fragments arranged by size:
3079 1485 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2844 2929 748
Size: 2096 85 3898
Fragments arranged by size:
3898 2096 85
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 2170 3404 2170
Size: 1234 4845
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2115 5193 5377 1305
Size: 810 3078 184 2007
Fragments arranged by size:
3078 2007 810 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4257
Fragments arranged by size:
4257 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4037 4396 4555 518
Size: 648 2871 359 159 2042
Fragments arranged by size:
2871 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2362 3495 4172 5320 5565 1606
Size: 756 1133 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 756 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 2489 387
Size: 2102 3977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 3305 3437 4982 1132
Size: 97 2076 132 1545 2229
Fragments arranged by size:
2229 2076 1545 132 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 1108
Size: 933 5146
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3336 1613
Size: 1723 4356
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 2112 2112
Size: 6079
PstI C_TGCA'G
Cuts at: 830 3431 830
Size: 2601 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2362 2362
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 4327 6039 4327
Size: 1712 4367
SfcI C'TryA_G
Cuts at: 826 1045 3427 4562 5243 5434 826
Size: 219 2382 1135 681 191 1471
Fragments arranged by size:
2382 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3296 4183 5084 5346 820
Size: 197 926 1353 887 901 262 1553
Fragments arranged by size:
1553 1353 926 901 887 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 3437 1132
Size: 2305 3774
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 3266 1517
Size: 1749 4330
StyI C'CwwG_G
Cuts at: 513 1065 2004 2446 3518 513
Size: 552 939 442 1072 3074
Fragments arranged by size:
3074 1072 939 552 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4106 4123 4276 4461 4906 325
Size: 1255 2526 17 153 185 445 1498
Fragments arranged by size:
2526 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2744 4325 6037
Size: 274 80 33 51 1443 767 1581 1712
Cuts at: 6037 96
Size: 138
Fragments arranged by size:
1712 1581 1443 767 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6039 6071 6039
Size: 32 6047
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 2483 2510 2762 2941 4208 2483
Size: 27 252 179 1267 4354
Fragments arranged by size:
4354 1267 252 179 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI