Restriction map for
pFN21AB7786
(Circular) (Six-base) MAPSORT of: pFN21AB7786.seq Check: 1368 from: 1 to: 6241
pFN21AB7786 6241 bp
With 182 enzymes: SgfI *
April 15, 2010 09:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3369 3477 4304 5688 5956 1632
Size: 1737 108 827 1384 268 1917
Fragments arranged by size:
1917 1737 1384 827 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 5140 6117 1203
Size: 3937 977 1327
Fragments arranged by size:
3937 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 2995 1166
Size: 1829 4412
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6241
AlwNI CAG_nnn'CTG
Cuts at: 2603 2871 3530 3533 3984 5556 2603
Size: 268 659 3 451 1572 3288
Fragments arranged by size:
3288 1572 659 451 268 3
ApaI G_GGCC'C
Cuts at: 2284 2284
Size: 6241
ApaLI G'TGCA_C
Cuts at: 2421 3384 4298 5454 2421
Size: 963 914 1156 3208
Fragments arranged by size:
3208 1156 963 914
ApoI r'AATT_y
Cuts at: 1493 2192 2345 3550 3776 3928 6037 1493
Size: 699 153 1205 226 152 2109 1697
Fragments arranged by size:
2109 1697 1205 699 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 3462 3566 1870
Size: 73 1519 104 4545
Fragments arranged by size:
4545 1519 104 73
BamHI G'GATC_C
Cuts at: 2206 2307 2623 3169 3571 3938 2206
Size: 101 316 546 402 367 4509
Fragments arranged by size:
4509 546 402 367 316 101
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2610 3263 3562 5017 618
Size: 325 217 627 823 653 299 1455 1842
Fragments arranged by size:
1842 1455 823 653 627 325 299 217
BanII G_rGCy'C
Cuts at: 1391 2284 2855 2979 3381 3560 1391
Size: 893 571 124 402 179 4072
Fragments arranged by size:
4072 893 571 402 179 124
BbeI G_GCGC'C
Cuts at: 2614 2614
Size: 6241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3357 928
Size: 971 1458 3812
Fragments arranged by size:
3812 1458 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BfrBI ATG'CAT
Cuts at: 3007 3007
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2232 4852 136
Size: 107 122 71 631 605 560 2620 1525
Fragments arranged by size:
2620 1525 631 605 560 122 107 71
BglII A'GATC_T
Cuts at: 6236 6236
Size: 6241
BlpI GC'TnA_GC
Cuts at: 2273 2596 3470 3658 2273
Size: 323 874 188 4856
Fragments arranged by size:
4856 874 323 188
Bme1580I G_kGCm'C
Cuts at: 1790 2284 2425 3230 3312 3388 4302 5458 1790
Size: 494 141 805 82 76 914 1156 2573
Fragments arranged by size:
2573 1156 914 805 494 141 82 76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 3375 3972
Size: 416 53 83 399 24 660 1298 597
Cuts at: 3972 4930 442
Size: 958 1753
Fragments arranged by size:
1753 1298 958 660 597 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2520 1056
Size: 1464 4777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2606 2620 3359 4901 1239
Size: 911 456 14 739 1542 2579
Fragments arranged by size:
2579 1542 911 739 456 14
Bpu10I CC'TnA_GC
Cuts at: 2849 2849
Size: 6241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2901 3201 4366 5231 5529 2901
Size: 300 1165 865 298 3613
Fragments arranged by size:
3613 1165 865 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4904 882
Size: 617 3405 2219
Fragments arranged by size:
3405 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4032 493
Size: 711 2828 2702
Fragments arranged by size:
2828 2702 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2611 4048 4430 5065
Size: 53 83 186 936 1078 1437 382 635
Cuts at: 5065 275
Size: 1451
Fragments arranged by size:
1451 1437 1078 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4674 5072 5346 5493 5904 4674
Size: 398 274 147 411 5011
Fragments arranged by size:
5011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2464 2494 1178
Size: 30 165 30 1061 30 4925
Fragments arranged by size:
4925 1061 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2670 3029 3107 3221 1186
Size: 1484 359 78 114 4206
Fragments arranged by size:
4206 1484 359 114 78
BseYI C'CCAG_C
Cuts at: 5444 5940 5444
Size: 496 5745
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 3270 1163
Size: 573 780 754 4134
Fragments arranged by size:
4134 780 754 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2425 3213 3388 4302 4387 5458 2425
Size: 788 175 914 85 1071 3208
Fragments arranged by size:
3208 1071 914 788 175 85
BsmI GAATG_Cn'
Cuts at: 2647 3752 3845 2647
Size: 1105 93 5043
Fragments arranged by size:
5043 1105 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2284 2425 2855 2979 3213 3230 3312
Size: 399 494 141 430 124 234 17 82
Cuts at: 3312 3381 3388 3560 4302 4387 5458 1391
Size: 69 7 172 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 430 399 234 172
141 124 85 82 69 17 7
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3163 3596 844
Size: 2319 433 3489
Fragments arranged by size:
3489 2319 433
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 1394
Size: 429 2305 3507
Fragments arranged by size:
3507 2305 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4736 4910 65
Size: 1183 3488 174 1396
Fragments arranged by size:
3488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2012 4885 5072 1700
Size: 110 202 2873 187 2869
Fragments arranged by size:
2873 2869 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 3195 4301 5313 3195
Size: 1106 1012 4123
Fragments arranged by size:
4123 1106 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2244 2871 794
Size: 1450 627 4164
Fragments arranged by size:
4164 1450 627
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 3297 3297
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 2106 2206 2307 2623 3169 3571 3938 4323
Size: 134 100 101 316 546 402 367 385
Cuts at: 4323 4340 5792 5878 5890 6236 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 546 402 385 367 346 316
134 101 100 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2889 3012 3182 2889
Size: 123 170 5948
Fragments arranged by size:
5948 170 123
BtgI C'CryG_G
Cuts at: 513 1065 2767 3120 513
Size: 552 1702 353 3634
Fragments arranged by size:
3634 1702 552 353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2149 487
Size: 683 56 923 4579
Fragments arranged by size:
4579 923 683 56
BtsI GCAGTG_nn'
Cuts at: 2990 3002 3757 4551 4571 2990
Size: 12 755 794 20 4660
Fragments arranged by size:
4660 794 755 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2388 2423 529
Size: 35 1824 35 4347
Fragments arranged by size:
4347 1824 35 35
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5248 809
Size: 507 3932 1802
Fragments arranged by size:
3932 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2536 2665 2764
Size: 54 1425 50 275 18 706 129 99
Cuts at: 2764 3688 4577 6206 8
Size: 924 889 1629 43
Fragments arranged by size:
1629 1425 924 889 706 275 129 99
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2142 2449 3216 3246 4171 1353
Size: 789 307 767 30 925 3423
Fragments arranged by size:
3423 925 789 767 307 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 3076 4828 5202 5348 387
Size: 1064 138 555 932 1752 374 146 1280
Fragments arranged by size:
1752 1280 1064 932 555 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2606 2620 3359 3369 3477 4304
Size: 393 518 456 14 739 10 108 827
Cuts at: 4304 4901 5688 5956 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 827 787 739 597 518 456 393
268 108 14 10
EcoO109I rG'GnC_Cy
Cuts at: 2281 3506 2281
Size: 1225 5016
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 2614 3442 5388 1678
Size: 936 828 1946 2531
Fragments arranged by size:
2531 1946 936 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2615 2647 2653 2685 4882
Size: 32 135 32 1043 32 6 32 2197
Cuts at: 4882 4914 4956 4988 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2197 1043 135 42 32 32 32
32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3021 3585 3831 669
Size: 988 281 1083 564 246 3079
Fragments arranged by size:
3079 1083 988 564 281 246
HindIII A'AGCT_T
Cuts at: 748 2919 3422 748
Size: 2171 503 3567
Fragments arranged by size:
3567 2171 503
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KasI G'GCGC_C
Cuts at: 2610 2610
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2507 5355 5539 1305
Size: 1202 2848 184 2007
Fragments arranged by size:
2848 2007 1202 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2538 2667 10
Size: 54 1425 343 706 129 3584
Fragments arranged by size:
3584 1425 706 343 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2995 4199 4558 4717 518
Size: 648 1829 1204 359 159 2042
Fragments arranged by size:
2042 1829 1204 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2600 2769 2871 3530 3657 4334 5482
Size: 507 487 169 102 659 127 677 1148
Cuts at: 5482 5727 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 659 507 487 245 169
127 102
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 6241
NarI GG'CG_CC
Cuts at: 2611 2611
Size: 6241
NcoI C'CATG_G
Cuts at: 513 1065 3120 513
Size: 552 2055 3634
Fragments arranged by size:
3634 2055 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 6241
NheI G'CTAG_C
Cuts at: 1052 2516 1052
Size: 1464 4777
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NsiI A_TGCA'T
Cuts at: 3009 3009
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 3599 5144 1132
Size: 97 2370 1545 2229
Fragments arranged by size:
2370 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2972 3511 1348
Size: 1624 539 4078
Fragments arranged by size:
4078 1624 539
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2762 3056 3517 1108
Size: 1654 294 461 3832
Fragments arranged by size:
3832 1654 461 294
PfoI T'CCnGG_A
Cuts at: 2127 2636 2127
Size: 509 5732
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3506 3506
Size: 6241
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PspOMI G'GGCC_C
Cuts at: 2280 2280
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2142 2997 3045 3593 830
Size: 1312 855 48 548 3478
Fragments arranged by size:
3478 1312 855 548 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2113 2600 2871 3530 2113
Size: 487 271 659 4824
Fragments arranged by size:
4824 659 487 271
SacII CC_GC'GG
Cuts at: 2770 2770
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SfcI C'TryA_G
Cuts at: 826 1045 2017 2138 2830 2993 3041 3589 4724
Size: 219 972 121 692 163 48 548 1135
Cuts at: 4724 5405 5596 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 972 692 681 548 219 191
163 121 48
SfoI GGC'GCC
Cuts at: 2612 2612
Size: 6241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3568 1872
Size: 1696 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2916 3216 4345 5246 5508 820
Size: 197 926 973 300 1129 901 262 1553
Fragments arranged by size:
1553 1129 973 926 901 300 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 2847 3367 1517
Size: 1330 520 4391
Fragments arranged by size:
4391 1330 520
StyI C'CwwG_G
Cuts at: 513 1065 3120 3680 513
Size: 552 2055 560 3074
Fragments arranged by size:
3074 2055 560 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2385 2489 3307 4268 4285 4438 4623
Size: 1255 805 104 818 961 17 153 185
Cuts at: 4623 5068 325
Size: 445 1498
Fragments arranged by size:
1498 1255 961 818 805 445 185 153
104 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4487 6199 96
Size: 274 80 33 51 1443 2510 1712 138
Fragments arranged by size:
2510 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2464 2496 6201 6233 2464
Size: 32 3705 32 2472
Fragments arranged by size:
3705 2472 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6241
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3118 3118
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 3566 1870
Size: 1696 4545
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NruI PacI PmlI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI