Restriction map for
pFN21AB7785
(Circular) (Six-base) MAPSORT of: pFN21AB7785.seq Check: 4516 from: 1 to: 6076
pFN21AB7785 6076 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2518 3419 1937
Size: 581 901 4594
Fragments arranged by size:
4594 901 581
Acc65I G'GTAC_C
Cuts at: 3397 3397
Size: 6076
AclI AA'CG_TT
Cuts at: 1563 4203 4576 1563
Size: 2640 373 3063
Fragments arranged by size:
3063 2640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 3169 3253 4139 5523 5791 1632
Size: 425 1112 84 886 1384 268 1917
Fragments arranged by size:
1917 1384 1112 886 425 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5879
AflIII A'CryG_T
Cuts at: 1203 4975 5952 1203
Size: 3772 977 1327
Fragments arranged by size:
3772 1327 977
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 4805 4805
Size: 6076
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6076
AlwNI CAG_nnn'CTG
Cuts at: 3819 5391 3819
Size: 1572 4504
ApaLI G'TGCA_C
Cuts at: 4133 5289 4133
Size: 1156 4920
ApoI r'AATT_y
Cuts at: 1493 2322 2799 3051 3337 3385 3611 3763 5872
Size: 829 477 252 286 48 226 152 2109
Cuts at: 5872 1493
Size: 1697
Fragments arranged by size:
2109 1697 829 477 286 252 226 152
48
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 3401 1870
Size: 73 1458 4545
Fragments arranged by size:
4545 1458 73
BamHI G'GATC_C
Cuts at: 3406 3773 3406
Size: 367 5709
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3397 4852 618
Size: 325 217 627 1610 1455 1842
Fragments arranged by size:
1842 1610 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3281 3395 1391
Size: 1890 114 4072
Fragments arranged by size:
4072 1890 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4267 4301 1903
Size: 34 2330 34 3678
Fragments arranged by size:
3678 2330 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3968 5178 3968
Size: 1210 4866
BclI T'GATC_A
Cuts at: 1548 2310 1548
Size: 762 5314
BfrBI ATG'CAT
Cuts at: 2573 2913 3113 2573
Size: 340 200 5536
Fragments arranged by size:
5536 340 200
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4687 136
Size: 107 122 71 631 605 3015 1525
Fragments arranged by size:
3015 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2224 6071 2224
Size: 3847 2229
BlpI GC'TnA_GC
Cuts at: 3493 3493
Size: 6076
Bme1580I G_kGCm'C
Cuts at: 1790 4137 5293 1790
Size: 2347 1156 2573
Fragments arranged by size:
2573 2347 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2147 2296 2733
Size: 416 53 83 399 24 730 149 437
Cuts at: 2733 3807 4765 442
Size: 1074 958 1753
Fragments arranged by size:
1753 1074 958 730 437 416 399 149
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6076
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3086
Size: 6076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 2913 3081 3165 3249 3295 4736 1239
Size: 1338 336 168 84 84 46 1441 2579
Fragments arranged by size:
2579 1441 1338 336 168 84 84 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4201 5066 5364 4201
Size: 865 298 4913
Fragments arranged by size:
4913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2137 4739 882
Size: 617 638 2602 2219
Fragments arranged by size:
2602 2219 638 617
BsaAI yAC'GTr
Cuts at: 493 1204 3867 493
Size: 711 2663 2702
Fragments arranged by size:
2702 2663 711
BsaBI GATnn'nnATC
Cuts at: 6070 6070
Size: 6076
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3883 4265 4900 275
Size: 53 83 186 936 2350 382 635 1451
Fragments arranged by size:
2350 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4509 4907 5181 5328 5739 4509
Size: 398 274 147 411 4846
Fragments arranged by size:
4846 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5851
Fragments arranged by size:
5851 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5190
BseYI C'CCAG_C
Cuts at: 2200 5279 5775 2200
Size: 3079 496 2501
Fragments arranged by size:
3079 2501 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5503
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3526 4287 5315 1540
Size: 275 186 1525 761 1028 2301
Fragments arranged by size:
2301 1525 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3281 4137 4222 5293 3281
Size: 856 85 1071 4064
Fragments arranged by size:
4064 1071 856 85
BsmI GAATG_Cn'
Cuts at: 2915 3587 3680 2915
Size: 672 93 5311
Fragments arranged by size:
5311 672 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3281 3395 4137 4222 5293 1391
Size: 399 1491 114 742 85 1071 2174
Fragments arranged by size:
2174 1491 1071 742 399 114 85
BspEI T'CCGG_A
Cuts at: 5739 5739
Size: 6076
BspHI T'CATG_A
Cuts at: 3965 5695 3965
Size: 1730 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3431 844
Size: 2587 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3963 1394
Size: 429 2140 3507
Fragments arranged by size:
3507 2140 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4571 4745 65
Size: 1183 3323 174 1396
Fragments arranged by size:
3323 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4720 4907 1700
Size: 110 2910 187 2869
Fragments arranged by size:
2910 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2094 96
Size: 1998 4078
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 4136 5148 4136
Size: 1012 5064
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6076
BstBI TT'CG_AA
Cuts at: 4880 4880
Size: 6076
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6076
BstXI CCAn_nnnn'nTGG
Cuts at: 2501 2501
Size: 6076
BstYI r'GATC_y
Cuts at: 1972 2224 3406 3773 4158 4175 5627 5713 5725
Size: 252 1182 367 385 17 1452 86 12
Cuts at: 5725 6071 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 252 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5524
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5337
Fragments arranged by size:
5337 683 56
BtsI GCAGTG_nn'
Cuts at: 2860 3592 4386 4406 2860
Size: 732 794 20 4530
Fragments arranged by size:
4530 794 732 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 2356 3380 2356
Size: 1024 5052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 809
Size: 507 3767 1802
Fragments arranged by size:
3767 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2122 3523
Size: 54 1425 50 275 18 207 85 1401
Cuts at: 3523 4412 6041 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1401 889 275 207 85 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3523 1537
Size: 275 1711 4090
Fragments arranged by size:
4090 1711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3128 4006 1353
Size: 1775 878 3423
Fragments arranged by size:
3423 1775 878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4663 5037 5183 387
Size: 1064 138 3074 374 146 1280
Fragments arranged by size:
3074 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3279 3279
Size: 6076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2577 2913 3081 3165 3169 3249
Size: 393 425 520 336 168 84 4 80
Cuts at: 3249 3253 3295 4139 4736 5523 5791 1239
Size: 4 42 844 597 787 268 1524
Fragments arranged by size:
1524 844 787 597 520 425 393 336
268 168 84 80 42 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2336 2481 2336
Size: 145 5931
EcoRI G'AATT_C
Cuts at: 2322 2799 3051 3385 3763 2322
Size: 477 252 334 378 4635
Fragments arranged by size:
4635 477 378 334 252
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 5223 1678
Size: 3545 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2314 3295 3327 4717
Size: 32 135 32 710 32 981 32 1390
Cuts at: 4717 4749 4791 4823 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1390 981 710 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2519 3420 3666 669
Size: 988 281 581 901 246 3079
Fragments arranged by size:
3079 988 901 581 281 246
HindIII A'AGCT_T
Cuts at: 748 3012 748
Size: 2264 3812
HpaI GTT'AAC
Cuts at: 3666 3666
Size: 6076
KpnI G_GTAC'C
Cuts at: 3401 3401
Size: 6076
MfeI C'AATT_G
Cuts at: 3675 3675
Size: 6076
MluI A'CGCG_T
Cuts at: 5952 5952
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 2802 5190 5374 1305
Size: 786 711 2388 184 2007
Fragments arranged by size:
2388 2007 786 711 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 2124 10
Size: 54 1425 343 207 85 3962
Fragments arranged by size:
3962 1425 343 207 85 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2499 2572 4034 4393 4552 518
Size: 648 1333 73 1462 359 159 2042
Fragments arranged by size:
2042 1462 1333 648 359 159 73
MspA1I CmG'CkG
Cuts at: 1606 3273 3492 4169 5317 5562 1606
Size: 1667 219 677 1148 245 2120
Fragments arranged by size:
2120 1667 1148 677 245 219
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5524
NdeI CA'TA_TG
Cuts at: 387 2457 2569 387
Size: 2070 112 3894
Fragments arranged by size:
3894 2070 112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6076
NotI GC'GGCC_GC
Cuts at: 3523 3523
Size: 6076
NsiI A_TGCA'T
Cuts at: 2575 2915 3115 2575
Size: 340 200 5536
Fragments arranged by size:
5536 340 200
NspI r_CATG'y
Cuts at: 1132 1229 2577 3113 3434 4979 1132
Size: 97 1348 536 321 1545 2229
Fragments arranged by size:
2229 1545 1348 536 321 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6076
PciI A'CATG_T
Cuts at: 4975 4975
Size: 6076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6076
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 5658 5690 4145
Size: 32 1481 32 4531
Fragments arranged by size:
4531 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6076
PsiI TTA'TAA
Cuts at: 3646 3646
Size: 6076
PstI C_TGCA'G
Cuts at: 830 3428 830
Size: 2598 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6076
PvuII CAG'CTG
Cuts at: 3273 3273
Size: 6076
SacI G_AGCT'C
Cuts at: 3281 3281
Size: 6076
SalI G'TCGA_C
Cuts at: 1936 2517 3418 1936
Size: 581 901 4594
Fragments arranged by size:
4594 901 581
SbfI CC_TGCA'GG
Cuts at: 3428 3428
Size: 6076
ScaI AGT'ACT
Cuts at: 2470 4324 6036 2470
Size: 1854 1712 2510
Fragments arranged by size:
2510 1854 1712
SexAI A'CCwGG_T
Cuts at: 3372 3372
Size: 6076
SfcI C'TryA_G
Cuts at: 826 1045 3424 4559 5240 5431 826
Size: 219 2379 1135 681 191 1471
Fragments arranged by size:
2379 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6076
SmaI CCC'GGG
Cuts at: 1872 3403 1872
Size: 1531 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4180 5081 5343 820
Size: 197 926 2237 901 262 1553
Fragments arranged by size:
2237 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 3113 3434 1132
Size: 1981 321 3774
Fragments arranged by size:
3774 1981 321
SspI AAT'ATT
Cuts at: 5 52 4000 5
Size: 47 3948 2081
Fragments arranged by size:
3948 2081 47
StuI AGG'CCT
Cuts at: 1517 2762 1517
Size: 1245 4831
StyI C'CwwG_G
Cuts at: 513 1065 3515 513
Size: 552 2450 3074
Fragments arranged by size:
3074 2450 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4103 4120 4273 4458 4903 325
Size: 1255 2523 17 153 185 445 1498
Fragments arranged by size:
2523 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 2468 2744
Size: 274 80 33 51 1443 117 374 276
Cuts at: 2744 4322 6034 96
Size: 1578 1712 138
Fragments arranged by size:
1712 1578 1443 374 276 274 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6036 6068 6036
Size: 32 6044
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6076
XbaI T'CTAG_A
Cuts at: 3412 3412
Size: 6076
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6076
XmaI C'CCGG_G
Cuts at: 1870 3401 1870
Size: 1531 4545
XmnI GAAnn'nnTTC
Cuts at: 2322 2868 3014 4205 2322
Size: 546 146 1191 4193
Fragments arranged by size:
4193 1191 546 146
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI