Restriction map for
pFN21AB7782
(Circular) (Six-base) MAPSORT of: pFN21AB7782.seq Check: 9796 from: 1 to: 6235
pFN21AB7782 6235 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3045 3045
Size: 6235
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3578 1937
Size: 1641 4594
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2126 2462 2465 2888 4298 5682 5950
Size: 416 78 336 3 423 1410 1384 268
Cuts at: 5950 1632
Size: 1917
Fragments arranged by size:
1917 1410 1384 423 416 336 268 78
3
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 2957 5066 2957
Size: 2109 4126
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 2960 1166
Size: 1794 4441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3043 1184
Size: 1859 4376
AlwNI CAG_nnn'CTG
Cuts at: 2664 2866 3978 5550 2664
Size: 202 1112 1572 3349
Fragments arranged by size:
3349 1572 1112 202
ApaI G_GGCC'C
Cuts at: 2131 3281 3486 2131
Size: 1150 205 4880
Fragments arranged by size:
4880 1150 205
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 3544 3770 3922 6031 1493
Size: 2051 226 152 2109 1697
Fragments arranged by size:
2109 2051 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 2674 2699 3560 1870
Size: 73 731 25 861 4545
Fragments arranged by size:
4545 861 731 73 25
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3216 3260 3329 3389 3523
Size: 325 217 627 1429 44 69 60 134
Cuts at: 3523 3556 5011 618
Size: 33 1455 1842
Fragments arranged by size:
1842 1455 1429 627 325 217 134 69
60 44 33
BanII G_rGCy'C
Cuts at: 1391 2118 2131 2492 2712 3150 3281 3300 3486
Size: 727 13 361 220 438 131 19 186
Cuts at: 3486 3554 1391
Size: 68 4072
Fragments arranged by size:
4072 727 438 361 220 186 131 68
19 13
BbeI G_GCGC'C
Cuts at: 3264 3393 3264
Size: 129 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2876 3049 3471 928
Size: 971 977 173 422 3692
Fragments arranged by size:
3692 977 971 422 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2239 2716
Size: 107 122 71 631 605 337 230 477
Cuts at: 2716 3258 3363 4846 136
Size: 542 105 1483 1525
Fragments arranged by size:
1525 1483 631 605 542 477 337 230
122 107 105 71
BglII A'GATC_T
Cuts at: 6230 6230
Size: 6235
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2295 3012 3101 3221 3281 3334 3486
Size: 341 164 717 89 120 60 53 152
Cuts at: 3486 4296 5452 1790
Size: 810 1156 2573
Fragments arranged by size:
2573 1156 810 717 341 164 152 120
89 60 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2369 3966 4924
Size: 416 53 83 399 24 952 1597 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1753 1597 958 952 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2524 4895 1239
Size: 878 407 2371 2579
Fragments arranged by size:
2579 2371 878 407
Bpu10I CC'TnA_GC
Cuts at: 2160 3164 2160
Size: 1004 5231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 4360 5225 5523 2369
Size: 1991 865 298 3081
Fragments arranged by size:
3081 1991 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2186 3017 4898 882
Size: 617 687 831 1881 2219
Fragments arranged by size:
2219 1881 831 687 617
BsaAI yAC'GTr
Cuts at: 493 1204 4026 493
Size: 711 2822 2702
Fragments arranged by size:
2822 2702 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2510 3051 3180 3261
Size: 53 83 186 936 977 541 129 81
Cuts at: 3261 3288 3390 4042 4424 5059 275
Size: 27 102 652 382 635 1451
Fragments arranged by size:
1451 977 936 652 635 541 382 186
129 102 83 81 53 27
BsaWI w'CCGG_w
Cuts at: 2532 2957 4668 5066 5340 5487 5898 2532
Size: 425 1711 398 274 147 411 2869
Fragments arranged by size:
2869 1711 425 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2083 2113 2477 2507 2609
Size: 30 165 30 680 30 364 30 102
Cuts at: 2609 2639 2672 2702 1178
Size: 30 33 30 4711
Fragments arranged by size:
4711 680 364 165 102 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3140 3455 3458 3461 3470 3473 1186
Size: 1954 315 3 3 9 3 3948
Fragments arranged by size:
3948 1954 315 9 3 3 3
BseYI C'CCAG_C
Cuts at: 3274 3311 5438 5934 3274
Size: 37 2127 496 3575
Fragments arranged by size:
3575 2127 496 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2591 1163
Size: 573 426 429 4807
Fragments arranged by size:
4807 573 429 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2278 3685 4446 5474 1540
Size: 275 186 277 1407 761 1028 2301
Fragments arranged by size:
2301 1407 1028 761 277 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2337 4296 4381 5452 2118
Size: 219 1959 85 1071 2901
Fragments arranged by size:
2901 1959 1071 219 85
BsmI GAATG_Cn'
Cuts at: 3746 3839 3746
Size: 93 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2131 2295 2337 2492 2712 3012
Size: 399 328 13 164 42 155 220 300
Cuts at: 3012 3101 3150 3221 3281 3300 3334 3486 3554
Size: 89 49 71 60 19 34 152 68
Cuts at: 3554 4296 4381 5452 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 300 220 164
155 152 89 85 71 68 60 49
42 34 19 13
BspEI T'CCGG_A
Cuts at: 2532 5898 2532
Size: 3366 2869
BspHI T'CATG_A
Cuts at: 4124 5854 4124
Size: 1730 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2563 3035 3045 3072 3590 844
Size: 1719 472 10 27 518 3489
Fragments arranged by size:
3489 1719 518 472 27 10
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 4122 1394
Size: 429 1504 795 3507
Fragments arranged by size:
3507 1504 795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4730 4904 65
Size: 1183 3482 174 1396
Fragments arranged by size:
3482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2238 2282 2957 3526 4879 5066 1700
Size: 110 428 44 675 569 1353 187 2869
Fragments arranged by size:
2869 1353 675 569 428 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 2111 3211 3269 4295 5307 2111
Size: 1100 58 1026 1012 3039
Fragments arranged by size:
3039 1100 1026 1012 58
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6235
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 3159 3565 3932 4317 4334 5786 5872 5884
Size: 1187 406 367 385 17 1452 86 12
Cuts at: 5884 6230 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1187 406 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2613 2613
Size: 6235
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4744
Fragments arranged by size:
4744 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2623 487
Size: 683 56 1397 4099
Fragments arranged by size:
4099 1397 683 56
BtsI GCAGTG_nn'
Cuts at: 3047 3751 4545 4565 3047
Size: 704 794 20 4717
Fragments arranged by size:
4717 794 704 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2502 2537 529
Size: 35 1938 35 4227
Fragments arranged by size:
4227 1938 35 35
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5242 809
Size: 507 3926 1802
Fragments arranged by size:
3926 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 3682 4571
Size: 54 1425 50 275 18 933 919 889
Cuts at: 4571 6200 8
Size: 1629 43
Fragments arranged by size:
1629 1425 933 919 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3447 3471 4165 1353
Size: 2094 24 694 3423
Fragments arranged by size:
3423 2094 694 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 2387 4822 5196 5342 387
Size: 1064 138 438 360 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 438 374 360 146 138
EcoICRI GAG'CTC
Cuts at: 2116 2116
Size: 6235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2117 2126 2462 2465 2524 2888
Size: 393 416 69 9 336 3 59 364
Cuts at: 2888 4298 4895 5682 5950 1239
Size: 1410 597 787 268 1524
Fragments arranged by size:
1524 1410 787 597 416 393 364 336
268 69 59 9 3
EcoO109I rG'GnC_Cy
Cuts at: 2127 2128 2312 3278 3482 3483 3517 2127
Size: 1 184 966 204 1 34 4845
Fragments arranged by size:
4845 966 204 184 34 1 1
EcoRI G'AATT_C
Cuts at: 3544 3922 3544
Size: 378 5857
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 2289 3264 3393 3499 5382 1678
Size: 611 975 129 106 1883 2531
Fragments arranged by size:
2531 1883 975 611 129 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3407 3439 4876 4908 4950
Size: 32 135 32 1835 32 1437 32 42
Cuts at: 4950 4982 1373
Size: 32 2626
Fragments arranged by size:
2626 1835 1437 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2653 3319 3579 3825 669
Size: 988 281 715 666 260 246 3079
Fragments arranged by size:
3079 988 715 666 281 260 246
HindIII A'AGCT_T
Cuts at: 748 2477 748
Size: 1729 4506
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KasI G'GCGC_C
Cuts at: 3260 3389 3260
Size: 129 6106
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 5349 5533 1305
Size: 777 3267 184 2007
Fragments arranged by size:
3267 2007 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2642 2960 3092 3269 4193 4552
Size: 648 873 603 318 132 177 924 359
Cuts at: 4552 4711 518
Size: 159 2042
Fragments arranged by size:
2042 924 873 648 603 359 318 177
159 132
MspA1I CmG'CkG
Cuts at: 1606 2137 2305 2607 2839 2986 3246 3501 3651
Size: 531 168 302 232 147 260 255 150
Cuts at: 3651 4328 5476 5721 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 302 260 255 245
232 168 150 147
NaeI GCC'GGC
Cuts at: 2284 3528 2284
Size: 1244 4991
NarI GG'CG_CC
Cuts at: 3261 3390 3261
Size: 129 6106
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4744
Fragments arranged by size:
4744 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NgoMIV G'CCGG_C
Cuts at: 2282 3526 2282
Size: 1244 4991
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3593 5138 1132
Size: 97 2364 1545 2229
Fragments arranged by size:
2364 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3334 1348
Size: 1986 4249
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6235
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PspOMI G'GGCC_C
Cuts at: 2127 3277 3482 2127
Size: 1150 205 4880
Fragments arranged by size:
4880 1150 205
PstI C_TGCA'G
Cuts at: 830 2553 2862 3042 3069 3251 3587 830
Size: 1723 309 180 27 182 336 3478
Fragments arranged by size:
3478 1723 336 309 182 180 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 2137 2607 2839 3246 2137
Size: 470 232 407 5126
Fragments arranged by size:
5126 470 407 232
SacI G_AGCT'C
Cuts at: 2118 2118
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 2348 4483 6195 2348
Size: 2135 1712 2388
Fragments arranged by size:
2388 2135 1712
SexAI A'CCwGG_T
Cuts at: 2826 3198 2826
Size: 372 5863
SfcI C'TryA_G
Cuts at: 826 1045 2152 2549 2858 3038 3065 3247 3583
Size: 219 1107 397 309 180 27 182 336
Cuts at: 3583 4718 5399 5590 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1107 681 397 336 309 219
191 182 180 27
SfoI GGC'GCC
Cuts at: 3262 3391 3262
Size: 129 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 2957 1700
Size: 1257 4978
SmaI CCC'GGG
Cuts at: 1872 2676 2701 3562 1872
Size: 804 25 861 4545
Fragments arranged by size:
4545 861 804 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 4339 5240 5502 820
Size: 197 926 441 1955 901 262 1553
Fragments arranged by size:
1955 1553 926 901 441 262 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 2320 4159 5
Size: 47 2268 1839 2081
Fragments arranged by size:
2268 2081 1839 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 2004 3674 513
Size: 552 939 1670 3074
Fragments arranged by size:
3074 1670 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2756 4262 4279 4432 4617 5062 325
Size: 1255 1176 1506 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 1176 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 4481 6193
Size: 274 80 33 51 1443 369 2135 1712
Cuts at: 6193 96
Size: 138
Fragments arranged by size:
2135 1712 1443 369 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2083 2115 2477 2509 3197 3229 6195 6227 2083
Size: 32 362 32 688 32 2966 32 2091
Fragments arranged by size:
2966 2091 688 362 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3053 1316
Size: 1737 4498
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2302 3271 2302
Size: 969 5266
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 2674 2699 3560 1870
Size: 804 25 861 4545
Fragments arranged by size:
4545 861 804 25
XmnI GAAnn'nnTTC
Cuts at: 4364 4364
Size: 6235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI