Restriction map for
pFN21AB7778
(Circular) (Six-base) MAPSORT of: pFN21AB7778.seq Check: 9460 from: 1 to: 6229
pFN21AB7778 6229 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3319 3319
Size: 6229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3194 4039 278
Size: 53 83 186 936 1658 845 2468
Fragments arranged by size:
2468 1658 936 845 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2588 3572 1937
Size: 651 984 4594
Fragments arranged by size:
4594 984 651
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 2470 2813 2993 4292 5676 5944 1632
Size: 686 152 343 180 1299 1384 268 1917
Fragments arranged by size:
1917 1384 1299 686 343 268 180 152
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 2254 4958 2254
Size: 2704 3525
AleI CACnn'nnGTG
Cuts at: 1166 3209 1166
Size: 2043 4186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6229
AlwNI CAG_nnn'CTG
Cuts at: 2554 2924 3972 5544 2554
Size: 370 1048 1572 3239
Fragments arranged by size:
3239 1572 1048 370
ApaI G_GGCC'C
Cuts at: 2163 2227 2346 2163
Size: 64 119 6046
Fragments arranged by size:
6046 119 64
ApaLI G'TGCA_C
Cuts at: 3089 4286 5442 3089
Size: 1197 1156 3876
Fragments arranged by size:
3876 1197 1156
ApoI r'AATT_y
Cuts at: 1493 3538 3764 3916 6025 1493
Size: 2045 226 152 2109 1697
Fragments arranged by size:
2109 2045 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2138 2156 2345 2424 3265 3554 1870
Size: 73 195 18 189 79 841 289 4545
Fragments arranged by size:
4545 841 289 195 189 79 73 18
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2209 2279 2914 3313 3550
Size: 325 217 627 422 70 635 399 237
Cuts at: 3550 5005 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 635 627 422 399 325 237
217 70
BanII G_rGCy'C
Cuts at: 1391 2051 2157 2163 2227 2346 3548 1391
Size: 660 106 6 64 119 1202 4072
Fragments arranged by size:
4072 1202 660 119 106 64 6
BbeI G_GCGC'C
Cuts at: 2213 2283 2213
Size: 70 6159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2426 2504 928
Size: 971 527 78 4653
Fragments arranged by size:
4653 971 527 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 1903
Size: 34 2483 34 3678
Fragments arranged by size:
3678 2483 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 2619 1548
Size: 1071 5158
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 4840 136
Size: 107 122 71 631 605 361 2807 1525
Fragments arranged by size:
2807 1525 631 605 361 122 107 71
BglII A'GATC_T
Cuts at: 2737 6224 2737
Size: 3487 2742
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 2163 2227 2346 3093 3204 4290 5446 1790
Size: 373 64 119 747 111 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 747 373 119 111 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2934 3065 3960
Size: 416 53 83 399 24 1517 131 895
Cuts at: 3960 4918 442
Size: 958 1753
Fragments arranged by size:
1753 1517 958 895 416 399 131 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 3139 4889 1239
Size: 953 947 1750 2579
Fragments arranged by size:
2579 1750 953 947
Bpu10I CC'TnA_GC
Cuts at: 2309 2309
Size: 6229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4354 5219 5517 4354
Size: 865 298 5066
Fragments arranged by size:
5066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2624 2771 4892 882
Size: 617 1125 147 2121 2219
Fragments arranged by size:
2219 2121 1125 617 147
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 2321 6223 2321
Size: 3902 2327
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 2280 2351 3191
Size: 53 83 186 936 677 70 71 840
Cuts at: 3191 3363 4036 4418 5053 275
Size: 172 673 382 635 1451
Fragments arranged by size:
1451 936 840 677 673 635 382 186
172 83 71 70 53
BsaWI w'CCGG_w
Cuts at: 2073 4662 5060 5334 5481 5892 2073
Size: 2589 398 274 147 411 2410
Fragments arranged by size:
2589 2410 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6004
Fragments arranged by size:
6004 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2279 2282 2285 2989 3142 1186
Size: 1093 3 3 704 153 4273
Fragments arranged by size:
4273 1093 704 153 3 3
BseYI C'CCAG_C
Cuts at: 2021 2275 3060 5432 5928 2021
Size: 254 785 2372 496 2322
Fragments arranged by size:
2372 2322 785 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3095 3422 1163
Size: 573 1359 327 3970
Fragments arranged by size:
3970 1359 573 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2093 2167 3679 4440 5468 1540
Size: 275 186 92 74 1512 761 1028 2301
Fragments arranged by size:
2301 1512 1028 761 275 186 92 74
BsiHKAI G_wGCw'C
Cuts at: 3093 4290 4375 5446 3093
Size: 1197 85 1071 3876
Fragments arranged by size:
3876 1197 1071 85
BsmI GAATG_Cn'
Cuts at: 3304 3740 3833 3304
Size: 436 93 5700
Fragments arranged by size:
5700 436 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3117 3117
Size: 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2157 2163 2227 2346 3093 3204
Size: 399 261 106 6 64 119 747 111
Cuts at: 3204 3548 4290 4375 5446 1391
Size: 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 399 344 261 119
111 106 85 64 6
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 2508 3186 4118 5848 2508
Size: 678 932 1730 2889
Fragments arranged by size:
2889 1730 932 678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 2826 3055 3319 3584 844
Size: 1819 163 229 264 265 3489
Fragments arranged by size:
3489 1819 265 264 229 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 2287 2388 2400 4116 1394
Size: 429 353 111 101 12 1716 3507
Fragments arranged by size:
3507 1716 429 353 111 101 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2380 2633 2797 4873 5060 1700
Size: 110 570 253 164 2076 187 2869
Fragments arranged by size:
2869 2076 570 253 187 164 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6229
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 2911 3211 2911
Size: 300 5929
BstYI r'GATC_y
Cuts at: 1972 2737 3405 3559 3926 4311 4328 5780 5866
Size: 765 668 154 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 765 668 385 367 346 154
86 17 12
BstZ17I GTA'TAC
Cuts at: 2589 2589
Size: 6229
BtgI C'CryG_G
Cuts at: 513 1065 2028 2217 513
Size: 552 963 189 4525
Fragments arranged by size:
4525 963 552 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 2776 2959 3060 3745 4539 4559 2776
Size: 183 101 685 794 20 4446
Fragments arranged by size:
4446 794 685 183 101 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2707 2742 529
Size: 35 2143 35 4016
Fragments arranged by size:
4016 2143 35 35
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2090 2164 2361
Size: 54 1425 50 275 18 260 74 197
Cuts at: 2361 2565 3217 3676 4565 6194 8
Size: 204 652 459 889 1629 43
Fragments arranged by size:
1629 1425 889 652 459 275 260 204
197 74 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2090 2164 3676 1537
Size: 275 278 74 1512 4090
Fragments arranged by size:
4090 1512 278 275 74
EarI CTCTTCn'nnn_
Cuts at: 1353 2724 2969 4159 1353
Size: 1371 245 1190 3423
Fragments arranged by size:
3423 1371 1190 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4816 5190 5336 387
Size: 1064 138 3227 374 146 1280
Fragments arranged by size:
3227 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2318 2470 2813 2993 3139 4292
Size: 393 560 126 152 343 180 146 1153
Cuts at: 4292 4889 5676 5944 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1153 787 597 560 393 343 268
180 152 146 126
EcoO109I rG'GnC_Cy
Cuts at: 2134 2223 2224 3007 3067 3097 2134
Size: 89 1 783 60 30 5266
Fragments arranged by size:
5266 783 89 60 30 1
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2213 2283 2476 5376 1678
Size: 535 70 193 2900 2531
Fragments arranged by size:
2900 2531 535 193 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 4870 4902 4944
Size: 32 135 32 668 32 2598 32 42
Cuts at: 4944 4976 1373
Size: 32 2626
Fragments arranged by size:
2626 2598 668 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KasI G'GCGC_C
Cuts at: 2209 2279 2209
Size: 70 6159
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2736 5343 5527 1305
Size: 1431 2607 184 2007
Fragments arranged by size:
2607 2007 1431 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2567 3219 10
Size: 54 1425 343 735 652 3020
Fragments arranged by size:
3020 1425 735 652 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3038 3086 3185 3209 3329 4187 4546
Size: 648 1872 48 99 24 120 858 359
Cuts at: 4546 4705 518
Size: 159 2042
Fragments arranged by size:
2042 1872 858 648 359 159 120 99
48 24
MspA1I CmG'CkG
Cuts at: 1606 2030 2192 2219 2235 2360 2883 2988 3019
Size: 424 162 27 16 125 523 105 31
Cuts at: 3019 3159 3645 4322 5470 5715 1606
Size: 140 486 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 523 486 424 245 162
140 125 105 31 27 16
NaeI GCC'GGC
Cuts at: 2799 2799
Size: 6229
NarI GG'CG_CC
Cuts at: 2210 2280 2210
Size: 70 6159
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5677
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NgoMIV G'CCGG_C
Cuts at: 2797 2797
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3245 3587 5132 1132
Size: 97 2016 342 1545 2229
Fragments arranged by size:
2229 2016 1545 342 97
PasI CC'CwG_GG
Cuts at: 1348 2056 1348
Size: 708 5521
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3436 1108
Size: 2328 3901
PfoI T'CCnGG_A
Cuts at: 3348 3348
Size: 6229
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 3067 2134
Size: 933 5296
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 2159 2223 2342 2159
Size: 64 119 6046
Fragments arranged by size:
6046 119 64
PstI C_TGCA'G
Cuts at: 830 2613 3581 830
Size: 1783 968 3478
Fragments arranged by size:
3478 1783 968
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 3019 3019
Size: 6229
SacII CC_GC'GG
Cuts at: 2031 2220 2031
Size: 189 6040
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SanDI GG'GwC_CC
Cuts at: 2134 2134
Size: 6229
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SexAI A'CCwGG_T
Cuts at: 2748 2748
Size: 6229
SfcI C'TryA_G
Cuts at: 826 1045 2230 2609 3485 3577 4712 5393 5584
Size: 219 1185 379 876 92 1135 681 191
Cuts at: 5584 826
Size: 1471
Fragments arranged by size:
1471 1185 1135 876 681 379 219 191
92
SfoI GGC'GCC
Cuts at: 2211 2281 2211
Size: 70 6159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 2140 2426 3556 1872
Size: 268 286 1130 4545
Fragments arranged by size:
4545 1130 286 268
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4333 5234 5496 820
Size: 197 926 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3245 3587 1132
Size: 2113 342 3774
Fragments arranged by size:
3774 2113 342
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 2519 1517
Size: 1002 5227
StyI C'CwwG_G
Cuts at: 513 1065 2781 3668 513
Size: 552 1716 887 3074
Fragments arranged by size:
3074 1716 887 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3203 3263 4256 4273 4426 4611 5056
Size: 1255 1623 60 993 17 153 185 445
Cuts at: 5056 325
Size: 1498
Fragments arranged by size:
1623 1498 1255 993 445 185 153 60
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2978 4475 6187
Size: 274 80 33 51 1443 1001 1497 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1712 1497 1443 1001 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3268 3356 3268
Size: 88 6141
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 2138 2424 3554 1870
Size: 268 286 1130 4545
Fragments arranged by size:
4545 1130 286 268
XmnI GAAnn'nnTTC
Cuts at: 3302 4358 3302
Size: 1056 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3192 4037 276
Size: 53 83 186 936 1658 845 2468
Fragments arranged by size:
2468 1658 936 845 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SapI SfiI SrfI SwaI