Restriction map for
pFN21AB7774
(Circular) (Six-base) MAPSORT of: pFN21AB7774.seq Check: 6443 from: 1 to: 6223
pFN21AB7774 6223 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3566 1937
Size: 1629 4594
Acc65I G'GTAC_C
Cuts at: 3544 3544
Size: 6223
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2584 3256 4286 5670 5938 1632
Size: 952 672 1030 1384 268 1917
Fragments arranged by size:
1917 1384 1030 952 672 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 5122 6099 1203
Size: 3919 977 1327
Fragments arranged by size:
3919 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 2314 2979 3063 1166
Size: 1148 665 84 4326
Fragments arranged by size:
4326 1148 665 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6223
AlwNI CAG_nnn'CTG
Cuts at: 3966 5538 3966
Size: 1572 4651
ApaLI G'TGCA_C
Cuts at: 3110 4280 5436 3110
Size: 1170 1156 3897
Fragments arranged by size:
3897 1170 1156
ApoI r'AATT_y
Cuts at: 1493 2970 3014 3138 3390 3532 3758 3910 6019
Size: 1477 44 124 252 142 226 152 2109
Cuts at: 6019 1493
Size: 1697
Fragments arranged by size:
2109 1697 1477 252 226 152 142 124
44
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 3548 1870
Size: 73 1605 4545
Fragments arranged by size:
4545 1605 73
BamHI G'GATC_C
Cuts at: 3126 3553 3920 3126
Size: 427 367 5429
Fragments arranged by size:
5429 427 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3544 4999 618
Size: 325 217 627 1757 1455 1842
Fragments arranged by size:
1842 1757 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3542 1391
Size: 2151 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4414 4448 1903
Size: 34 50 34 2393 34 3678
Fragments arranged by size:
3678 2393 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4115 5325 4115
Size: 1210 5013
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BfrBI ATG'CAT
Cuts at: 3226 3226
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4834 136
Size: 107 122 71 631 605 3162 1525
Fragments arranged by size:
3162 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2066 6218 2066
Size: 4152 2071
BlpI GC'TnA_GC
Cuts at: 3640 3640
Size: 6223
Bme1580I G_kGCm'C
Cuts at: 1790 3114 4284 5440 1790
Size: 1324 1170 1156 2573
Fragments arranged by size:
2573 1324 1170 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6223
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3954 4912 442
Size: 416 53 83 399 24 2537 958 1753
Fragments arranged by size:
2537 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 6223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2748 4883 1239
Size: 956 553 2135 2579
Fragments arranged by size:
2579 2135 956 553
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4348 5213 5511 4348
Size: 865 298 5060
Fragments arranged by size:
5060 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 2560 2728 3400 4886 882
Size: 617 672 389 168 672 1486 2219
Fragments arranged by size:
2219 1486 672 672 617 389 168
BsaAI yAC'GTr
Cuts at: 493 1204 4014 493
Size: 711 2810 2702
Fragments arranged by size:
2810 2702 711
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4030 4412 5047 275
Size: 53 83 186 936 2497 382 635 1451
Fragments arranged by size:
2497 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3300 4656 5054 5328 5475 5886 3300
Size: 1356 398 274 147 411 3637
Fragments arranged by size:
3637 1356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5998
Fragments arranged by size:
5998 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2262 2285 2490 1186
Size: 1076 23 205 4919
Fragments arranged by size:
4919 1076 205 23
BseYI C'CCAG_C
Cuts at: 5426 5922 5426
Size: 496 5727
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5650
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3673 4434 5462 1540
Size: 275 186 1672 761 1028 2301
Fragments arranged by size:
2301 1672 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3114 4284 4369 5440 3114
Size: 1170 85 1071 3897
Fragments arranged by size:
3897 1170 1071 85
BsmI GAATG_Cn'
Cuts at: 2582 3226 3734 3827 2582
Size: 644 508 93 4978
Fragments arranged by size:
4978 644 508 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3114 3542 4284 4369 5440 1391
Size: 399 1324 428 742 85 1071 2174
Fragments arranged by size:
2174 1324 1071 742 428 399 85
BspEI T'CCGG_A
Cuts at: 5886 5886
Size: 6223
BspHI T'CATG_A
Cuts at: 2497 4112 5842 2497
Size: 1615 1730 2878
Fragments arranged by size:
2878 1730 1615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3578 844
Size: 2734 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4110 1394
Size: 429 2287 3507
Fragments arranged by size:
3507 2287 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2329 2619 4718 4892 65
Size: 1183 1081 290 2099 174 1396
Fragments arranged by size:
2099 1396 1183 1081 290 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4867 5054 1700
Size: 110 3057 187 2869
Fragments arranged by size:
3057 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3500 96
Size: 3404 2819
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 4283 5295 4283
Size: 1012 5211
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6223
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6223
BstYI r'GATC_y
Cuts at: 1972 2066 2300 3126 3553 3920 4305 4322 5774
Size: 94 234 826 427 367 385 17 1452
Cuts at: 5774 5860 5872 6218 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 826 427 385 367 346 234
94 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2281 2348 2281
Size: 67 6156
BtgI C'CryG_G
Cuts at: 513 1065 2287 513
Size: 552 1222 4449
Fragments arranged by size:
4449 1222 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5484
Fragments arranged by size:
5484 683 56
BtsI GCAGTG_nn'
Cuts at: 2309 3739 4533 4553 2309
Size: 1430 794 20 3979
Fragments arranged by size:
3979 1430 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 3527 3527
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5230 809
Size: 507 3914 1802
Fragments arranged by size:
3914 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 3670 4559
Size: 54 1425 50 275 18 384 1456 889
Cuts at: 4559 6188 8
Size: 1629 43
Fragments arranged by size:
1629 1456 1425 889 384 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2247 4153 1353
Size: 894 1906 3423
Fragments arranged by size:
3423 1906 894
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4810 5184 5330 387
Size: 1064 138 3221 374 146 1280
Fragments arranged by size:
3221 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2584 2748 3256 4286 4883 5670
Size: 393 563 389 164 508 1030 597 787
Cuts at: 5670 5938 1239
Size: 268 1524
Fragments arranged by size:
1524 1030 787 597 563 508 393 389
268 164
EcoO109I rG'GnC_Cy
Cuts at: 2652 2652
Size: 6223
EcoRI G'AATT_C
Cuts at: 2970 3138 3390 3532 3910 2970
Size: 168 252 142 378 5283
Fragments arranged by size:
5283 378 252 168 142
EcoRV GAT'ATC
Cuts at: 2865 2865
Size: 6223
FspI TGC'GCA
Cuts at: 4729 4729
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 5370 1678
Size: 3692 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2261 2293 2601 2633 4864
Size: 32 135 32 689 32 308 32 2231
Cuts at: 4864 4896 4938 4970 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2231 689 308 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3296 3567 3813 669
Size: 988 281 1358 271 246 3079
Fragments arranged by size:
3079 1358 988 281 271 246
HindIII A'AGCT_T
Cuts at: 748 2511 3435 748
Size: 1763 924 3536
Fragments arranged by size:
3536 1763 924
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KpnI G_GTAC'C
Cuts at: 3548 3548
Size: 6223
MfeI C'AATT_G
Cuts at: 3822 3822
Size: 6223
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 5337 5521 1305
Size: 915 3117 184 2007
Fragments arranged by size:
3117 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 10
Size: 54 1425 343 384 4017
Fragments arranged by size:
4017 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2163 2314 2358 2502 2979 3063 4181
Size: 648 997 151 44 144 477 84 1118
Cuts at: 4181 4540 4699 518
Size: 359 159 2042
Fragments arranged by size:
2042 1118 997 648 477 359 159 151
144 84 44
MspA1I CmG'CkG
Cuts at: 1606 3639 4316 5464 5709 1606
Size: 2033 677 1148 245 2120
Fragments arranged by size:
2120 2033 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2287 513
Size: 552 1222 4449
Fragments arranged by size:
4449 1222 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6223
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6223
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NsiI A_TGCA'T
Cuts at: 3228 3228
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 3581 5126 1132
Size: 97 2352 1545 2229
Fragments arranged by size:
2352 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6223
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2015 1108
Size: 907 5316
PfoI T'CCnGG_A
Cuts at: 2050 2050
Size: 6223
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 5805 5837 4292
Size: 32 1481 32 4678
Fragments arranged by size:
4678 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PstI C_TGCA'G
Cuts at: 830 3575 830
Size: 2745 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SapI GCTCTTCn'nnn_
Cuts at: 2247 2247
Size: 6223
SbfI CC_TGCA'GG
Cuts at: 3575 3575
Size: 6223
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SfcI C'TryA_G
Cuts at: 826 1045 2182 2518 3571 4706 5387 5578 826
Size: 219 1137 336 1053 1135 681 191 1471
Fragments arranged by size:
1471 1137 1135 1053 681 336 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 3550 1872
Size: 1678 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4327 5228 5490 820
Size: 197 926 2384 901 262 1553
Fragments arranged by size:
2384 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4014 493
Size: 3521 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6223
StyI C'CwwG_G
Cuts at: 513 1065 2287 3662 513
Size: 552 1222 1375 3074
Fragments arranged by size:
3074 1375 1222 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2485 4250 4267 4420 4605 5050 325
Size: 1255 905 1765 17 153 185 445 1498
Fragments arranged by size:
1765 1498 1255 905 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2747 3500 4469
Size: 274 80 33 51 1443 770 753 969
Cuts at: 4469 6181 96
Size: 1712 138
Fragments arranged by size:
1712 1443 969 770 753 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2688 2720 6183 6215 2688
Size: 32 3463 32 2696
Fragments arranged by size:
3463 2696 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6223
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6223
XmaI C'CCGG_G
Cuts at: 1870 3548 1870
Size: 1678 4545
XmnI GAAnn'nnTTC
Cuts at: 2429 2933 3017 3101 3269 3437 4352 2429
Size: 504 84 84 168 168 915 4300
Fragments arranged by size:
4300 915 504 168 168 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI