Restriction map for
pFN21AB7768
(Circular) (Six-base) MAPSORT of: pFN21AB7768.seq Check: 3126 from: 1 to: 5935
pFN21AB7768 5935 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2303 2303
Size: 5935
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3165 3745 278
Size: 53 83 186 936 1629 580 2468
Fragments arranged by size:
2468 1629 936 580 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 2451 3256 2451
Size: 805 5130
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2070 2124 2295 2318 3998 5382 5650 1632
Size: 438 54 171 23 1680 1384 268 1917
Fragments arranged by size:
1917 1680 1384 438 268 171 54 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 2460 4834 5811 1203
Size: 1257 2374 977 1327
Fragments arranged by size:
2374 1327 1257 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 3120 1166
Size: 1954 3981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2525 2557 2525
Size: 32 5903
AlwNI CAG_nnn'CTG
Cuts at: 2062 2152 2523 3678 5250 2062
Size: 90 371 1155 1572 2747
Fragments arranged by size:
2747 1572 1155 371 90
ApaI G_GGCC'C
Cuts at: 2029 2124 3034 2029
Size: 95 910 4930
Fragments arranged by size:
4930 910 95
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2528 3260 1870
Size: 73 585 732 4545
Fragments arranged by size:
4545 732 585 73
BamHI G'GATC_C
Cuts at: 3265 3632 3265
Size: 367 5568
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2451 3256 4711 618
Size: 325 217 627 664 805 1455 1842
Fragments arranged by size:
1842 1455 805 664 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 2124 2634 2760 3034 3254 1391
Size: 638 95 510 126 274 220 4072
Fragments arranged by size:
4072 638 510 274 220 126 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2058 928
Size: 971 159 4805
Fragments arranged by size:
4805 971 159
BbvCI CC'TCA_GC
Cuts at: 2063 2063
Size: 5935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4126 4160 1903
Size: 34 50 34 2105 34 3678
Fragments arranged by size:
3678 2105 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4546 136
Size: 107 122 71 631 605 2874 1525
Fragments arranged by size:
2874 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 2628 3352 2628
Size: 724 5211
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2124 3034 3196 3996 5152 1790
Size: 239 95 910 162 800 1156 2573
Fragments arranged by size:
2573 1156 910 800 239 162 95
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3666 4624 442
Size: 416 53 83 399 24 2249 958 1753
Fragments arranged by size:
2249 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2591 2774 2887 4595 1239
Size: 1352 183 113 1708 2579
Fragments arranged by size:
2579 1708 1352 183 113
Bpu10I CC'TnA_GC
Cuts at: 2063 2657 2702 2063
Size: 594 45 5296
Fragments arranged by size:
5296 594 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4060 4925 5223 4060
Size: 865 298 4772
Fragments arranged by size:
4772 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2952 3133 4598 882
Size: 617 1453 181 1465 2219
Fragments arranged by size:
2219 1465 1453 617 181
BsaAI yAC'GTr
Cuts at: 493 1204 2359 3726 493
Size: 711 1155 1367 2702
Fragments arranged by size:
2702 1367 1155 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2502 2578 2671 2694
Size: 53 83 186 936 969 76 93 23
Cuts at: 2694 3162 3742 4124 4759 275
Size: 468 580 382 635 1451
Fragments arranged by size:
1451 969 936 635 580 468 382 186
93 83 76 53 23
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 2189 2219 2726
Size: 30 165 30 621 30 135 30 507
Cuts at: 2726 2756 1178
Size: 30 4357
Fragments arranged by size:
4357 621 507 165 135 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2311 1186
Size: 1125 4810
BseYI C'CCAG_C
Cuts at: 3133 5138 5634 3133
Size: 2005 496 3434
Fragments arranged by size:
3434 2005 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2083 2737 2933 1163
Size: 573 347 654 196 4165
Fragments arranged by size:
4165 654 573 347 196
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2608 3385 4146 5174 1540
Size: 275 186 607 777 761 1028 2301
Fragments arranged by size:
2301 1028 777 761 607 275 186
BsiHKAI G_wGCw'C
Cuts at: 2760 3996 4081 5152 2760
Size: 1236 85 1071 3543
Fragments arranged by size:
3543 1236 1071 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 2438 2515 3169 2438
Size: 77 654 5204
Fragments arranged by size:
5204 654 77
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2124 2634 2760 3034 3196 3254
Size: 399 239 95 510 126 274 162 58
Cuts at: 3254 3996 4081 5152 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 274 239 162
126 95 85 58
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2303 3290 844
Size: 1459 987 3489
Fragments arranged by size:
3489 1459 987
BsrBI CCG'CTC
Cuts at: 1394 1823 2575 3822 1394
Size: 429 752 1247 3507
Fragments arranged by size:
3507 1247 752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2745 2947 4579 4766 1700
Size: 110 935 202 1632 187 2869
Fragments arranged by size:
2869 1632 935 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 2623 1925
Size: 698 5237
BssSI C'ACGA_G
Cuts at: 2198 3995 5007 2198
Size: 1797 1012 3126
Fragments arranged by size:
3126 1797 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5935
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 2851 1184
Size: 1667 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 2251 2251
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 3169 3265 3632 4017 4034 5486 5572 5584
Size: 1197 96 367 385 17 1452 86 12
Cuts at: 5584 5930 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1197 385 367 346 96 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3021 3060 3021
Size: 39 5896
BtgI C'CryG_G
Cuts at: 513 1065 2927 513
Size: 552 1862 3521
Fragments arranged by size:
3521 1862 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 2125 3451 4245 4265 2125
Size: 1326 794 20 3795
Fragments arranged by size:
3795 1326 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2340 2375 529
Size: 35 1776 35 4089
Fragments arranged by size:
4089 1776 35 35
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DraIII CAC_nnn'GTG
Cuts at: 2851 2851
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2507 3074 4942 809
Size: 507 1191 567 1868 1802
Fragments arranged by size:
1868 1802 1191 567 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2406 3382 4271
Size: 54 1425 50 275 18 576 976 889
Cuts at: 4271 5900 8
Size: 1629 43
Fragments arranged by size:
1629 1425 976 889 576 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2600 3865 1353
Size: 984 263 1265 3423
Fragments arranged by size:
3423 1265 984 263
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2626 2813 4522 4896 5042 387
Size: 1064 138 1037 187 1709 374 146 1280
Fragments arranged by size:
1709 1280 1064 1037 374 187 146 138
EcoICRI GAG'CTC
Cuts at: 2758 2758
Size: 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 2124 2295 2318 2591 2774 2887
Size: 393 438 54 171 23 273 183 113
Cuts at: 2887 3998 4595 5382 5650 1239
Size: 1111 597 787 268 1524
Fragments arranged by size:
1524 1111 787 597 438 393 273 268
183 171 113 54 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2228 2228
Size: 5935
EcoO109I rG'GnC_Cy
Cuts at: 2025 2026 2121 2286 2737 2989 3031 3083 2025
Size: 1 95 165 451 252 42 52 4877
Fragments arranged by size:
4877 451 252 165 95 52 42 1
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2180 2180
Size: 5935
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 2822 2924 5082 1678
Size: 1144 102 2158 2531
Fragments arranged by size:
2531 2158 1144 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2661 2693 3000
Size: 32 135 32 452 32 605 32 307
Cuts at: 3000 3032 3161 3193 4576 4608 4650 4682 1373
Size: 32 129 32 1383 32 42 32 2626
Fragments arranged by size:
2626 1383 605 452 307 135 129 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 3279 3525 669
Size: 988 281 322 1019 246 3079
Fragments arranged by size:
3079 1019 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 2455 3260 2455
Size: 805 5130
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 2460 5811 2460
Size: 3351 2584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5049 5233 1305
Size: 3744 184 2007
Fragments arranged by size:
3744 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4113
Fragments arranged by size:
4113 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2681 3120 3893 4252 4411 518
Size: 648 1515 439 773 359 159 2042
Fragments arranged by size:
2042 1515 773 648 439 359 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2664 2917 2929 2974 3351 4028 5176
Size: 963 95 253 12 45 377 677 1148
Cuts at: 5176 5421 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 963 677 377 253 245 95
45 12
NaeI GCC'GGC
Cuts at: 2747 2747
Size: 5935
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5383
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5935
NgoMIV G'CCGG_C
Cuts at: 2745 2745
Size: 5935
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NruI TCG'CGA
Cuts at: 3181 3181
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 3293 4838 1132
Size: 97 2064 1545 2229
Fragments arranged by size:
2229 2064 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5935
PfoI T'CCnGG_A
Cuts at: 2598 2730 2598
Size: 132 5803
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PmlI CAC'GTG
Cuts at: 2359 2359
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2989 2989
Size: 5935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PspOMI G'GGCC_C
Cuts at: 2025 2120 3030 2025
Size: 95 910 4930
Fragments arranged by size:
4930 910 95
PstI C_TGCA'G
Cuts at: 830 2979 3287 830
Size: 2149 308 3478
Fragments arranged by size:
3478 2149 308
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2569 2917 2974 2569
Size: 348 57 5530
Fragments arranged by size:
5530 348 57
SacI G_AGCT'C
Cuts at: 2760 2760
Size: 5935
SacII CC_GC'GG
Cuts at: 2930 2930
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SapI GCTCTTCn'nnn_
Cuts at: 2600 2600
Size: 5935
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 2204 4183 5895 2204
Size: 1979 1712 2244
Fragments arranged by size:
2244 1979 1712
SexAI A'CCwGG_T
Cuts at: 2055 2847 2055
Size: 792 5143
SfcI C'TryA_G
Cuts at: 826 1045 2188 2515 2975 3187 3283 4418 5099
Size: 219 1143 327 460 212 96 1135 681
Cuts at: 5099 5290 826
Size: 191 1471
Fragments arranged by size:
1471 1143 1135 681 460 327 219 212
191 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 3262 1872
Size: 1390 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4039 4940 5202 820
Size: 197 926 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 2068 1517
Size: 551 5384
StyI C'CwwG_G
Cuts at: 513 1065 2073 3222 3374 513
Size: 552 1008 1149 152 3074
Fragments arranged by size:
3074 1149 1008 552 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3062 3962 3979 4132 4317 4762 325
Size: 1255 1482 900 17 153 185 445 1498
Fragments arranged by size:
1498 1482 1255 900 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 4181 5893
Size: 274 80 33 51 1443 225 1979 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
1979 1712 1443 274 225 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2189 2221 2726 2758 5895 5927 2189
Size: 32 505 32 3137 32 2197
Fragments arranged by size:
3137 2197 505 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2462 3229 2462
Size: 767 5168
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 3260 1870
Size: 1390 4545
XmnI GAAnn'nnTTC
Cuts at: 4064 4064
Size: 5935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3163 3743 276
Size: 53 83 186 936 1629 580 2468
Fragments arranged by size:
2468 1629 936 580 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I EcoRV FseI FspAI KasI NarI NsiI PacI
PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI