Restriction map for
pFN21AB7764
(Circular) (Six-base) MAPSORT of: pFN21AB7764.seq Check: 5922 from: 1 to: 6208
pFN21AB7764 6208 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3315 3315
Size: 6208
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2450 4271 5655 5923 1632
Size: 677 141 1821 1384 268 1917
Fragments arranged by size:
1917 1821 1384 677 268 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 2920 5039 2920
Size: 2119 4089
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 2522 1166
Size: 1356 4852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 3951 5523 3951
Size: 1572 4636
ApaI G_GGCC'C
Cuts at: 2101 2101
Size: 6208
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 3517 3743 3895 6004 1493
Size: 2024 226 152 2109 1697
Fragments arranged by size:
2109 2024 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2100 2140 2300 2702 3533 1870
Size: 73 119 38 40 160 402 831 4545
Fragments arranged by size:
4545 831 402 160 119 73 40 38
AvrII C'CTAG_G
Cuts at: 2842 2842
Size: 6208
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2114 2179 2225 2553 2584
Size: 325 217 627 327 65 46 328 31
Cuts at: 2584 3529 4984 618
Size: 945 1455 1842
Fragments arranged by size:
1842 1455 945 627 328 327 325 217
65 46 31
BanII G_rGCy'C
Cuts at: 1391 2101 2703 3293 3375 3527 1391
Size: 710 602 590 82 152 4072
Fragments arranged by size:
4072 710 602 590 152 82
BbeI G_GCGC'C
Cuts at: 2183 2229 2557 2588 2183
Size: 46 328 31 5803
Fragments arranged by size:
5803 328 46 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2649 928
Size: 971 750 4487
Fragments arranged by size:
4487 971 750
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2018 4100 5310 2018
Size: 2082 1210 2916
Fragments arranged by size:
2916 2082 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2201 3454 4819
Size: 107 122 71 631 605 529 1253 1365
Cuts at: 4819 136
Size: 1525
Fragments arranged by size:
1525 1365 1253 631 605 529 122 107
71
BglII A'GATC_T
Cuts at: 3048 6203 3048
Size: 3155 3053
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 2101 3450 4269 5425 1790
Size: 311 1349 819 1156 2573
Fragments arranged by size:
2573 1349 1156 819 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2952 2952
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2708 2922 4868 1239
Size: 1469 214 1946 2579
Fragments arranged by size:
2579 1946 1469 214
Bpu10I CC'TnA_GC
Cuts at: 2802 2802
Size: 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 3410 4333 5198 5496 2543
Size: 867 923 865 298 3255
Fragments arranged by size:
3255 923 867 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3123 3202 4871 882
Size: 617 1624 79 1669 2219
Fragments arranged by size:
2219 1669 1624 617 79
BsaAI yAC'GTr
Cuts at: 493 1204 3999 493
Size: 711 2795 2702
Fragments arranged by size:
2795 2702 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2180 2226 2554 2585
Size: 53 83 186 936 647 46 328 31
Cuts at: 2585 3474 4015 4397 5032 275
Size: 889 541 382 635 1451
Fragments arranged by size:
1451 936 889 647 635 541 382 328
186 83 53 46 31
BsaWI w'CCGG_w
Cuts at: 2920 4641 5039 5313 5460 5871 2920
Size: 1721 398 274 147 411 3257
Fragments arranged by size:
3257 1721 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3263 3293 3404 3434 1178
Size: 30 165 30 1860 30 111 30 3952
Fragments arranged by size:
3952 1860 165 111 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 2651 2666 2675 2705 2708 2711 2726
Size: 1435 30 15 9 30 3 3 15
Cuts at: 2726 2729 2774 3490 1186
Size: 3 45 716 3904
Fragments arranged by size:
3904 1435 716 45 30 30 15 15
9 3 3 3
BseYI C'CCAG_C
Cuts at: 2016 2020 2269 2816 3208 3286 3310 5411 5907
Size: 4 249 547 392 78 24 2101 496
Cuts at: 5907 2016
Size: 2317
Fragments arranged by size:
2317 2101 547 496 392 249 78 24
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 1163
Size: 573 591 5044
Fragments arranged by size:
5044 591 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 2205 2319 3138 3168 3239
Size: 275 186 195 9 114 819 30 71
Cuts at: 3239 3658 4419 5447 1540
Size: 419 761 1028 2301
Fragments arranged by size:
2301 1028 819 761 419 275 195 186
114 71 30 9
BsiHKAI G_wGCw'C
Cuts at: 2163 2420 2451 2703 4269 4354 5425 2163
Size: 257 31 252 1566 85 1071 2946
Fragments arranged by size:
2946 1566 1071 257 252 85 31
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
BsmBI CGTCTCn'nnnn_
Cuts at: 2976 2976
Size: 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2163 2420 2451 2703 3293 3375
Size: 399 311 62 257 31 252 590 82
Cuts at: 3375 3450 3527 4269 4354 5425 1391
Size: 75 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 590 399 311 257 252
85 82 77 75 62 31
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3315 3563 844
Size: 2471 248 3489
Fragments arranged by size:
3489 2471 248
BsrBI CCG'CTC
Cuts at: 1394 1823 3096 3185 3277 4095 1394
Size: 429 1273 89 92 818 3507
Fragments arranged by size:
3507 1273 818 429 92 89
BsrDI GCAATG_nn'
Cuts at: 65 1248 4703 4877 65
Size: 1183 3455 174 1396
Fragments arranged by size:
3455 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2550 2910 2920 3234 4852 5039 1700
Size: 110 740 360 10 314 1618 187 2869
Fragments arranged by size:
2869 1618 740 360 314 187 110 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 2274 2546 2825 1925
Size: 349 272 279 5308
Fragments arranged by size:
5308 349 279 272
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6208
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 3048 3538 3905 4290 4307 5759 5845 5857
Size: 1076 490 367 385 17 1452 86 12
Cuts at: 5857 6203 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1076 490 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2190 2244 2319 2958 3327 513
Size: 552 1125 54 75 639 369 3394
Fragments arranged by size:
3394 1125 639 552 369 75 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 3724 4518 4538 3724
Size: 794 20 5394
Fragments arranged by size:
5394 794 20
ClaI AT'CG_AT
Cuts at: 1554 2766 1554
Size: 1212 4996
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2202 2316
Size: 54 1425 50 275 18 363 9 114
Cuts at: 2316 2365 2493 2790 2912 2955 3165 3236 3362
Size: 49 128 297 122 43 210 71 126
Cuts at: 3362 3655 4544 6173 8
Size: 293 889 1629 43
Fragments arranged by size:
1629 1425 889 363 297 293 275 210
128 126 122 114 71 54 50 49
43 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2193 2202 2316 3165 3236 3655 1537
Size: 275 381 9 114 849 71 419 4090
Fragments arranged by size:
4090 849 419 381 275 114 71 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2643 4138 1353
Size: 1290 1495 3423
Fragments arranged by size:
3423 1495 1290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 3019 3136 3396 4795 5169
Size: 1064 138 438 992 117 260 1399 374
Cuts at: 5169 5315 387
Size: 146 1280
Fragments arranged by size:
1399 1280 1064 992 438 374 260 146
138 117
EcoICRI GAG'CTC
Cuts at: 2701 2701
Size: 6208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2450 2708 2922 4271 4868 5655
Size: 393 677 141 258 214 1349 597 787
Cuts at: 5655 5923 1239
Size: 268 1524
Fragments arranged by size:
1524 1349 787 677 597 393 268 258
214 141
EcoNI CCTnn'n_nnAGG
Cuts at: 2519 2561 2519
Size: 42 6166
EcoO109I rG'GnC_Cy
Cuts at: 2097 2145 2383 2734 2852 2097
Size: 48 238 351 118 5453
Fragments arranged by size:
5453 351 238 118 48
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2062 2183 2229 2557 2588 2945 3206 5355
Size: 384 121 46 328 31 357 261 2149
Cuts at: 5355 1678
Size: 2531
Fragments arranged by size:
2531 2149 384 357 328 261 121 46
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4849 4881 4923 4955 1373
Size: 32 135 32 3277 32 42 32 2626
Fragments arranged by size:
3277 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 2179 2225 2553 2584 2179
Size: 46 328 31 5803
Fragments arranged by size:
5803 328 46 31
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 5322 5506 1305
Size: 873 3144 184 2007
Fragments arranged by size:
3144 2007 873 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2367 2495 10
Size: 54 1425 343 535 128 3723
Fragments arranged by size:
3723 1425 535 343 128 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 2522 4166 4525 4684 518
Size: 648 1341 15 1644 359 159 2042
Fragments arranged by size:
2042 1644 1341 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2020 2072 2082 2192 2198 2246 2269 2321
Size: 414 52 10 110 6 48 23 52
Cuts at: 2321 3134 3208 3329 3368 3624 4301 5449 5694
Size: 813 74 121 39 256 677 1148 245
Cuts at: 5694 1606
Size: 2120
Fragments arranged by size:
2120 1148 813 677 414 256 245 121
110 74 52 52 48 39 23 10
6
NaeI GCC'GGC
Cuts at: 2552 2552
Size: 6208
NarI GG'CG_CC
Cuts at: 2180 2226 2554 2585 2180
Size: 46 328 31 5803
Fragments arranged by size:
5803 328 46 31
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5656
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NgoMIV G'CCGG_C
Cuts at: 2550 2550
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 2193 2202 3655 2193
Size: 9 1453 4746
Fragments arranged by size:
4746 1453 9
NspI r_CATG'y
Cuts at: 1132 1229 3566 5111 1132
Size: 97 2337 1545 2229
Fragments arranged by size:
2337 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6208
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3051 1108
Size: 1943 4265
PfoI T'CCnGG_A
Cuts at: 3319 3319
Size: 6208
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2145 2383 2852 2145
Size: 238 469 5501
Fragments arranged by size:
5501 469 238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 2097 2097
Size: 6208
PstI C_TGCA'G
Cuts at: 830 3039 3156 3560 830
Size: 2209 117 404 3478
Fragments arranged by size:
3478 2209 404 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2020 2269 2020
Size: 249 5959
SacI G_AGCT'C
Cuts at: 2703 2703
Size: 6208
SacII CC_GC'GG
Cuts at: 2193 2247 2322 3330 2193
Size: 54 75 1008 5071
Fragments arranged by size:
5071 1008 75 54
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SanDI GG'GwC_CC
Cuts at: 2145 2383 2852 2145
Size: 238 469 5501
Fragments arranged by size:
5501 469 238
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SexAI A'CCwGG_T
Cuts at: 2531 2531
Size: 6208
SfcI C'TryA_G
Cuts at: 826 1045 3035 3152 3394 3556 4691 5372 5563
Size: 219 1990 117 242 162 1135 681 191
Cuts at: 5563 826
Size: 1471
Fragments arranged by size:
1990 1471 1135 681 242 219 191 162
117
SfiI GGCCn_nnn'nGGCC
Cuts at: 2201 2201
Size: 6208
SfoI GGC'GCC
Cuts at: 2181 2227 2555 2586 2181
Size: 46 328 31 5803
Fragments arranged by size:
5803 328 46 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 2550 1700
Size: 850 5358
SmaI CCC'GGG
Cuts at: 1872 2064 2102 2302 3535 1872
Size: 192 38 200 1233 4545
Fragments arranged by size:
4545 1233 200 192 38
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 3425 4312 5213 5475 820
Size: 197 926 615 867 887 901 262 1553
Fragments arranged by size:
1553 926 901 887 867 615 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 2435 2847 1517
Size: 918 412 4878
Fragments arranged by size:
4878 918 412
StyI C'CwwG_G
Cuts at: 513 1065 2215 2472 2842 3402 3647 513
Size: 552 1150 257 370 560 245 3074
Fragments arranged by size:
3074 1150 560 552 370 257 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2102 2534 4235 4252 4405 4590 5035
Size: 1255 522 432 1701 17 153 185 445
Cuts at: 5035 325
Size: 1498
Fragments arranged by size:
1701 1498 1255 522 445 432 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4454 6166 96
Size: 274 80 33 51 1443 2477 1712 138
Fragments arranged by size:
2477 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2907 2939 3263 3295 6168 6200 2907
Size: 32 324 32 2873 32 2915
Fragments arranged by size:
2915 2873 324 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2062 2100 2300 3533 1870
Size: 192 38 200 1233 4545
Fragments arranged by size:
4545 1233 200 192 38
XmnI GAAnn'nnTTC
Cuts at: 4337 4337
Size: 6208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SapI SrfI
SwaI XcmI