Restriction map for
pFN21AB7759
(Circular) (Six-base) MAPSORT of: pFN21AB7759.seq Check: 9648 from: 1 to: 6046
pFN21AB7759 6046 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3389 1937
Size: 1452 4594
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 2552 2561 2894 4109 5493 5761 1632
Size: 576 344 9 333 1215 1384 268 1917
Fragments arranged by size:
1917 1384 1215 576 344 333 268 9
AfeI AGC'GCT
Cuts at: 2762 2762
Size: 6046
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 4945 5922 1203
Size: 3742 977 1327
Fragments arranged by size:
3742 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 3158 1166
Size: 1992 4054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2781 3297 1184
Size: 1597 516 3933
Fragments arranged by size:
3933 1597 516
AlwNI CAG_nnn'CTG
Cuts at: 2115 3789 5361 2115
Size: 1674 1572 2800
Fragments arranged by size:
2800 1674 1572
ApaI G_GGCC'C
Cuts at: 2026 2026
Size: 6046
ApaLI G'TGCA_C
Cuts at: 3338 4103 5259 3338
Size: 765 1156 4125
Fragments arranged by size:
4125 1156 765
ApoI r'AATT_y
Cuts at: 1493 3005 3355 3581 3733 5842 1493
Size: 1512 350 226 152 2109 1697
Fragments arranged by size:
2109 1697 1512 350 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2320 3371 1870
Size: 73 377 1051 4545
Fragments arranged by size:
4545 1051 377 73
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 3367 4822 618
Size: 325 217 627 938 642 1455 1842
Fragments arranged by size:
1842 1455 938 642 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 2193 2220 3365 1391
Size: 635 167 27 1145 4072
Fragments arranged by size:
4072 1145 635 167 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2882 2982 928
Size: 971 983 100 3992
Fragments arranged by size:
3992 983 971 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2412 4657 136
Size: 107 122 71 631 605 740 2245 1525
Fragments arranged by size:
2245 1525 740 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3327 6041 3327
Size: 2714 3332
BlpI GC'TnA_GC
Cuts at: 2621 2846 3463 2621
Size: 225 617 5204
Fragments arranged by size:
5204 617 225
Bme1580I G_kGCm'C
Cuts at: 1790 2026 3342 4107 5263 1790
Size: 236 1316 765 1156 2573
Fragments arranged by size:
2573 1316 1156 765 236
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2645 3777 4735
Size: 416 53 83 399 24 1228 1132 958
Cuts at: 4735 442
Size: 1753
Fragments arranged by size:
1753 1228 1132 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2924 3016 3116 4706 1239
Size: 959 726 92 100 1590 2579
Fragments arranged by size:
2579 1590 959 726 100 92
Bpu10I CC'TnA_GC
Cuts at: 3254 3254
Size: 6046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2936 4171 5036 5334 2936
Size: 1235 865 298 3648
Fragments arranged by size:
3648 1235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4709 882
Size: 617 3210 2219
Fragments arranged by size:
3210 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3837 493
Size: 711 2633 2702
Fragments arranged by size:
2702 2633 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3853 4235 4870 275
Size: 53 83 186 936 2320 382 635 1451
Fragments arranged by size:
2320 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3246 4479 4877 5151 5298 5709 3246
Size: 1233 398 274 147 411 3583
Fragments arranged by size:
3583 1233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2669 2699 2923 2953 1178
Size: 30 165 30 1266 30 224 30 4271
Fragments arranged by size:
4271 1266 224 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2513 2567 1186
Size: 1327 54 4665
Fragments arranged by size:
4665 1327 54
BseYI C'CCAG_C
Cuts at: 2017 2153 2281 2616 2664 5249 5745 2017
Size: 136 128 335 48 2585 496 2318
Fragments arranged by size:
2585 2318 496 335 136 128 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2819 3140 1163
Size: 573 1083 321 4069
Fragments arranged by size:
4069 1083 573 321
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2889 3496 4257 5285 1540
Size: 275 186 888 607 761 1028 2301
Fragments arranged by size:
2301 1028 888 761 607 275 186
BsiHKAI G_wGCw'C
Cuts at: 3342 4107 4192 5263 3342
Size: 765 85 1071 4125
Fragments arranged by size:
4125 1071 765 85
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
BsmBI CGTCTCn'nnnn_
Cuts at: 2797 3187 2797
Size: 390 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2193 2220 3342 3365 4107 4192
Size: 399 236 167 27 1122 23 742 85
Cuts at: 4192 5263 1391
Size: 1071 2174
Fragments arranged by size:
2174 1122 1071 742 399 236 167 85
27 23
BspEI T'CCGG_A
Cuts at: 3246 5709 3246
Size: 2463 3583
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3401 844
Size: 2557 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3081 4541 4715 65
Size: 1183 1833 1460 174 1396
Fragments arranged by size:
1833 1460 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2679 2697 3108 4690 4877 1700
Size: 110 869 18 411 1582 187 2869
Fragments arranged by size:
2869 1582 869 411 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 4106 5118 4106
Size: 1012 5034
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2084 2115 794
Size: 1290 31 4725
Fragments arranged by size:
4725 1290 31
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstXI CCAn_nnnn'nTGG
Cuts at: 2153 2281 2153
Size: 128 5918
BstYI r'GATC_y
Cuts at: 1972 2238 2523 2731 3327 3376 3743 4128 4145
Size: 266 285 208 596 49 367 385 17
Cuts at: 4145 5597 5683 5695 6041 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 596 385 367 346 285 266
208 86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2427 2721 3218 513
Size: 552 1362 294 497 3341
Fragments arranged by size:
3341 1362 552 497 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 2419 3284 3562 4356 4376 2419
Size: 865 278 794 20 4089
Fragments arranged by size:
4089 865 794 278 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2416 2784 3493
Size: 54 1425 50 275 18 586 368 709
Cuts at: 3493 4382 6011 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 709 586 368 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3976 1353
Size: 2623 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2360 2516 4633 5007 5153 387
Size: 1064 138 771 156 2117 374 146 1280
Fragments arranged by size:
2117 1280 1064 771 374 156 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2208 2552 2561 2894 2924 3016
Size: 393 566 10 344 9 333 30 92
Cuts at: 3016 3116 4109 4706 5493 5761 1239
Size: 100 993 597 787 268 1524
Fragments arranged by size:
1524 993 787 597 566 393 344 333
268 100 92 30 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2126 2126
Size: 6046
EcoO109I rG'GnC_Cy
Cuts at: 2022 2023 2022
Size: 1 6045
EcoRI G'AATT_C
Cuts at: 3005 3355 3733 3005
Size: 350 378 5318
Fragments arranged by size:
5318 378 350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 6046
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 2686 2764 5193 1678
Size: 1008 78 2429 2531
Fragments arranged by size:
2531 2429 1008 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 4687 4719 4761
Size: 32 135 32 731 32 2352 32 42
Cuts at: 4761 4793 1373
Size: 32 2626
Fragments arranged by size:
2626 2352 731 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2812 3390 3636 669
Size: 988 281 874 578 246 3079
Fragments arranged by size:
3079 988 874 578 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5160 5344 1305
Size: 3855 184 2007
Fragments arranged by size:
3855 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 10
Size: 54 1425 343 954 3270
Fragments arranged by size:
3270 1425 954 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2334 2438 3156 3158 3198 4004 4363
Size: 648 1168 104 718 2 40 806 359
Cuts at: 4363 4522 518
Size: 159 2042
Fragments arranged by size:
2042 1168 806 718 648 359 159 104
40 2
MspA1I CmG'CkG
Cuts at: 1606 2115 3316 3462 4139 5287 5532 1606
Size: 509 1201 146 677 1148 245 2120
Fragments arranged by size:
2120 1201 1148 677 509 245 146
NcoI C'CATG_G
Cuts at: 513 1065 2427 2721 513
Size: 552 1362 294 3838
Fragments arranged by size:
3838 1362 552 294
NdeI CA'TA_TG
Cuts at: 387 3157 387
Size: 2770 3276
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 2443 2494 3176 3404 4949 1132
Size: 97 1214 51 682 228 1545 2229
Fragments arranged by size:
2229 1545 1214 682 228 97 51
PasI CC'CwG_GG
Cuts at: 1348 2101 2586 2927 1348
Size: 753 485 341 4467
Fragments arranged by size:
4467 753 485 341
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 2967 3616 2967
Size: 649 5397
PspOMI G'GGCC_C
Cuts at: 2022 2022
Size: 6046
PstI C_TGCA'G
Cuts at: 830 2167 2188 2844 3106 3150 3297 3398 830
Size: 1337 21 656 262 44 147 101 3478
Fragments arranged by size:
3478 1337 656 262 147 101 44 21
PvuI CG_AT'CG
Cuts at: 2001 2889 2001
Size: 888 5158
PvuII CAG'CTG
Cuts at: 2115 2115
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2163 2184 2840 3102 3146 3293 3394
Size: 219 1118 21 656 262 44 147 101
Cuts at: 3394 4529 5210 5401 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1118 681 656 262 219 191
147 101 44 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 3373 1872
Size: 1501 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2951 4150 5051 5313 820
Size: 197 926 1008 1199 901 262 1553
Fragments arranged by size:
1553 1199 1008 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 2494 3176 3404 1132
Size: 1362 682 228 3774
Fragments arranged by size:
3774 1362 682 228
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 2318 1517
Size: 801 5245
StyI C'CwwG_G
Cuts at: 513 1065 2427 2714 2721 3485 513
Size: 552 1362 287 7 764 3074
Fragments arranged by size:
3074 1362 764 552 287 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3219 4073 4090 4243 4428 4873 325
Size: 1255 1639 854 17 153 185 445 1498
Fragments arranged by size:
1639 1498 1255 854 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4292 6004 96
Size: 274 80 33 51 1443 2315 1712 138
Fragments arranged by size:
2315 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6006 6038 6006
Size: 32 6014
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 3371 1870
Size: 1501 4545
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI