Restriction map for
pFN21AB7756
(Circular) (Six-base) MAPSORT of: pFN21AB7756.seq Check: 5699 from: 1 to: 6199
pFN21AB7756 6199 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2174 3542 1937
Size: 237 1368 4594
Fragments arranged by size:
4594 1368 237
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2107 2397 2593 2677 2761 2845 3013 3097
Size: 475 290 196 84 84 84 168 84
Cuts at: 3097 3181 3265 4262 5646 5914 1632
Size: 84 84 997 1384 268 1917
Fragments arranged by size:
1917 1384 997 475 290 268 196 168
84 84 84 84 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 5098 6075 1203
Size: 3895 977 1327
Fragments arranged by size:
3895 1327 977
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 3410 1166
Size: 2244 3955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6199
AlwNI CAG_nnn'CTG
Cuts at: 2039 2616 2868 2931 3036 3942 5514 2039
Size: 577 252 63 105 906 1572 2724
Fragments arranged by size:
2724 1572 906 577 252 105 63
ApaI G_GGCC'C
Cuts at: 2106 2106
Size: 6199
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 2895 3275 3508 3734 3886 5995 1493
Size: 1402 380 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1402 380 233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4753 160
Size: 872 3721 1606
Fragments arranged by size:
3721 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 3524 1870
Size: 73 163 1418 4545
Fragments arranged by size:
4545 1418 163 73
BamHI G'GATC_C
Cuts at: 2111 3529 3896 2111
Size: 1418 367 4414
Fragments arranged by size:
4414 1418 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2163 3520 4975 618
Size: 325 217 627 376 1357 1455 1842
Fragments arranged by size:
1842 1455 1357 627 376 325 217
BanII G_rGCy'C
Cuts at: 1391 2106 2705 3293 3377 3518 1391
Size: 715 599 588 84 141 4072
Fragments arranged by size:
4072 715 599 588 141 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2316 928
Size: 971 417 4811
Fragments arranged by size:
4811 971 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4390 4424 1903
Size: 34 50 34 2369 34 3678
Fragments arranged by size:
3678 2369 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2196 4091 5301 2196
Size: 1895 1210 3094
Fragments arranged by size:
3094 1895 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6199
BfrBI ATG'CAT
Cuts at: 2585 2585
Size: 6199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6194 6194
Size: 6199
BlpI GC'TnA_GC
Cuts at: 2872 3292 3616 2872
Size: 420 324 5455
Fragments arranged by size:
5455 420 324
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2496 4260 5416 1790
Size: 316 390 1764 1156 2573
Fragments arranged by size:
2573 1764 1156 390 316
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6199
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2263 3165 3930
Size: 416 53 83 399 24 846 902 765
Cuts at: 3930 4888 442
Size: 958 1753
Fragments arranged by size:
1753 958 902 846 765 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2724 2724
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2313 2321 2673 2757 4859 1239
Size: 791 283 8 352 84 2102 2579
Fragments arranged by size:
2579 2102 791 352 283 84 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 4324 5189 5487 2465
Size: 1859 865 298 3177
Fragments arranged by size:
3177 1859 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2093 2458 2500 4862 882
Size: 617 594 365 42 2362 2219
Fragments arranged by size:
2362 2219 617 594 365 42
BsaAI yAC'GTr
Cuts at: 493 1204 3990 493
Size: 711 2786 2702
Fragments arranged by size:
2786 2702 711
BsaBI GATnn'nnATC
Cuts at: 6193 6193
Size: 6199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4006 4388 5023 275
Size: 53 83 186 936 2473 382 635 1451
Fragments arranged by size:
2473 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2819 2903 3323 3450 4632 5030 5304 5451 5862
Size: 84 420 127 1182 398 274 147 411
Cuts at: 5862 2819
Size: 3156
Fragments arranged by size:
3156 1182 420 411 398 274 147 127
84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 1186
Size: 895 5304
BseYI C'CCAG_C
Cuts at: 2099 2250 5402 5898 2099
Size: 151 3152 496 2400
Fragments arranged by size:
3152 2400 496 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2692 2776 3028 3196 3475 1163
Size: 573 956 84 252 168 279 3887
Fragments arranged by size:
3887 956 573 279 252 168 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3649 4410 5438 1540
Size: 275 186 1648 761 1028 2301
Fragments arranged by size:
2301 1648 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2705 3293 3377 4260 4345 5416 2705
Size: 588 84 883 85 1071 3488
Fragments arranged by size:
3488 1071 883 588 85 84
BsmI GAATG_Cn'
Cuts at: 2791 2927 3431 3710 3803 2791
Size: 136 504 279 93 5187
Fragments arranged by size:
5187 504 279 136 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2496 2705 3293 3377 3518 4260
Size: 399 316 390 209 588 84 141 742
Cuts at: 4260 4345 5416 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 399 390 316 209
141 85 84
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 4088 5818 4088
Size: 1730 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2322 3554 844
Size: 1478 1232 3489
Fragments arranged by size:
3489 1478 1232
BsrBI CCG'CTC
Cuts at: 1394 1823 4086 1394
Size: 429 2263 3507
Fragments arranged by size:
3507 2263 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4694 4868 65
Size: 1183 3446 174 1396
Fragments arranged by size:
3446 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4843 5030 1700
Size: 110 3033 187 2869
Fragments arranged by size:
3033 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6199
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 2168 4259 5271 2168
Size: 2091 1012 3096
Fragments arranged by size:
3096 2091 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6199
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6199
BstYI r'GATC_y
Cuts at: 1972 2111 3529 3896 4281 4298 5750 5836 5848
Size: 139 1418 367 385 17 1452 86 12
Cuts at: 5848 6194 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1418 385 367 346 139 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2180 2428 2496 513
Size: 552 1115 248 68 4216
Fragments arranged by size:
4216 1115 552 248 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5460
Fragments arranged by size:
5460 683 56
BtsI GCAGTG_nn'
Cuts at: 2134 2680 2764 3715 4509 4529 2134
Size: 546 84 951 794 20 3804
Fragments arranged by size:
3804 951 794 546 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 3503 3503
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3646 4535 6164
Size: 54 1425 50 275 18 1816 889 1629
Cuts at: 6164 8
Size: 43
Fragments arranged by size:
1816 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 2636 2764 4129 1353
Size: 1091 192 128 1365 3423
Fragments arranged by size:
3423 1365 1091 192 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4786 5160 5306 387
Size: 1064 138 3197 374 146 1280
Fragments arranged by size:
3197 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2703 3291 3375 2703
Size: 588 84 5527
Fragments arranged by size:
5527 588 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2107 2313 2321 2397 2593 2673
Size: 393 398 77 206 8 76 196 80
Cuts at: 2673 2677 2757 2761 2845 3013 3097 3181 3265
Size: 4 80 4 84 168 84 84 84
Cuts at: 3265 4262 4859 5646 5914 1239
Size: 997 597 787 268 1524
Fragments arranged by size:
1524 997 787 597 398 393 268 206
196 168 84 84 84 84 80 80
77 76 8 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2031 2031
Size: 6199
EcoO109I rG'GnC_Cy
Cuts at: 2036 2103 2036
Size: 67 6132
EcoRI G'AATT_C
Cuts at: 2895 3508 3886 2895
Size: 613 378 5208
Fragments arranged by size:
5208 613 378
FspI TGC'GCA
Cuts at: 3040 4705 3040
Size: 1665 4534
HaeII r_GCGC'y
Cuts at: 1678 5346 1678
Size: 3668 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2457 2475 2489 3196
Size: 32 135 32 871 14 18 14 707
Cuts at: 3196 3228 4840 4872 4914 4946 1373
Size: 32 1612 32 42 32 2626
Fragments arranged by size:
2626 1612 871 707 135 42 32 32
32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3543 3789 669
Size: 988 281 1605 246 3079
Fragments arranged by size:
3079 1605 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2379 748
Size: 1631 4568
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 5313 5497 1305
Size: 948 3060 184 2007
Fragments arranged by size:
3060 2007 948 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4377
Fragments arranged by size:
4377 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2584 3256 3410 4157 4516 4675 518
Size: 648 1418 672 154 747 359 159 2042
Fragments arranged by size:
2042 1418 747 672 648 359 159 154
MspA1I CmG'CkG
Cuts at: 1606 3615 4292 5440 5685 1606
Size: 2009 677 1148 245 2120
Fragments arranged by size:
2120 2009 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5647
NdeI CA'TA_TG
Cuts at: 387 2581 387
Size: 2194 4005
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NsiI A_TGCA'T
Cuts at: 2587 2587
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 2589 3557 5102 1132
Size: 97 1360 968 1545 2229
Fragments arranged by size:
2229 1545 1360 968 97
PasI CC'CwG_GG
Cuts at: 1348 2414 1348
Size: 1066 5133
PciI A'CATG_T
Cuts at: 5098 5098
Size: 6199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6199
PfoI T'CCnGG_A
Cuts at: 2216 2216
Size: 6199
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5781 5813 4268
Size: 32 1481 32 4654
Fragments arranged by size:
4654 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PspOMI G'GGCC_C
Cuts at: 2102 2102
Size: 6199
PstI C_TGCA'G
Cuts at: 830 3551 830
Size: 2721 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6199
SacI G_AGCT'C
Cuts at: 2705 3293 3377 2705
Size: 588 84 5527
Fragments arranged by size:
5527 588 84
SalI G'TCGA_C
Cuts at: 1936 3541 1936
Size: 1605 4594
SapI GCTCTTCn'nnn_
Cuts at: 2444 2636 2764 2444
Size: 192 128 5879
Fragments arranged by size:
5879 192 128
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 2022 4447 6159 2022
Size: 2425 1712 2062
Fragments arranged by size:
2425 2062 1712
SfcI C'TryA_G
Cuts at: 826 1045 2833 3085 3547 4682 5363 5554 826
Size: 219 1788 252 462 1135 681 191 1471
Fragments arranged by size:
1788 1471 1135 681 462 252 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 2108 3526 1872
Size: 236 1418 4545
Fragments arranged by size:
4545 1418 236
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 4303 5204 5466 820
Size: 197 926 501 1859 901 262 1553
Fragments arranged by size:
1859 1553 926 901 501 262 197
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 4123 5
Size: 47 4071 2081
Fragments arranged by size:
4071 2081 47
StuI AGG'CCT
Cuts at: 1517 2690 2942 3026 3110 3194 3446 1517
Size: 1173 252 84 84 84 252 4270
Fragments arranged by size:
4270 1173 252 252 84 84 84
StyI C'CwwG_G
Cuts at: 513 1065 3638 513
Size: 552 2573 3074
Fragments arranged by size:
3074 2573 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4226 4243 4396 4581 5026 325
Size: 1255 2646 17 153 185 445 1498
Fragments arranged by size:
2646 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2020 4445 6157
Size: 274 80 33 51 1443 43 2425 1712
Cuts at: 6157 96
Size: 138
Fragments arranged by size:
2425 1712 1443 274 138 80 51 43
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6159 6191 6159
Size: 32 6167
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6199
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 2106 3524 1870
Size: 236 1418 4545
Fragments arranged by size:
4545 1418 236
XmnI GAAnn'nnTTC
Cuts at: 2453 2690 2858 2942 3026 3110 3194 3278 3446
Size: 237 168 84 84 84 84 84 168
Cuts at: 3446 4328 2453
Size: 882 4324
Fragments arranged by size:
4324 882 237 168 168 84 84 84
84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI