Restriction map for
pFN21AB7748
(Circular) (Six-base) MAPSORT of: pFN21AB7748.seq Check: 851 from: 1 to: 6169
pFN21AB7748 6169 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2029 3135 3295 4232 5616 5884 1632
Size: 397 1106 160 937 1384 268 1917
Fragments arranged by size:
1917 1384 1106 937 397 268 160
AfeI AGC'GCT
Cuts at: 3424 3424
Size: 6169
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 2766 5068 6045 1203
Size: 1563 2302 977 1327
Fragments arranged by size:
2302 1563 1327 977
AgeI A'CCGG_T
Cuts at: 2615 5000 2615
Size: 2385 3784
AhdI GACnn_n'nnGTC
Cuts at: 3148 4898 3148
Size: 1750 4419
AleI CACnn'nnGTG
Cuts at: 1166 2294 2490 1166
Size: 1128 196 4845
Fragments arranged by size:
4845 1128 196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3184 3216 3184
Size: 32 6137
AlwNI CAG_nnn'CTG
Cuts at: 2052 2338 3912 5484 2052
Size: 286 1574 1572 2737
Fragments arranged by size:
2737 1574 1572 286
ApaLI G'TGCA_C
Cuts at: 2636 4226 5382 2636
Size: 1590 1156 3423
Fragments arranged by size:
3423 1590 1156
ApoI r'AATT_y
Cuts at: 1493 2528 2558 2740 3478 3704 3856 5965 1493
Size: 1035 30 182 738 226 152 2109 1697
Fragments arranged by size:
2109 1697 1035 738 226 182 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2430 2458 3437 3494 1870
Size: 73 487 28 979 57 4545
Fragments arranged by size:
4545 979 487 73 57 28
BamHI G'GATC_C
Cuts at: 3499 3866 3499
Size: 367 5802
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2302 2438 3242 3380 3490
Size: 325 217 627 515 136 804 138 110
Cuts at: 3490 4945 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 804 627 515 325 217 138
136 110
BanII G_rGCy'C
Cuts at: 1391 2237 3070 3104 3488 1391
Size: 846 833 34 384 4072
Fragments arranged by size:
4072 846 833 384 34
BbeI G_GCGC'C
Cuts at: 2306 2442 3246 3384 2306
Size: 136 804 138 5091
Fragments arranged by size:
5091 804 138 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2187 2221 2687 2721 4360 4394 1903
Size: 34 250 34 466 34 1639 34 3678
Fragments arranged by size:
3678 1639 466 250 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2021 2088 3372
Size: 107 122 71 631 605 349 67 1284
Cuts at: 3372 4780 136
Size: 1408 1525
Fragments arranged by size:
1525 1408 1284 631 605 349 122 107
71 67
BglII A'GATC_T
Cuts at: 2596 6164 2596
Size: 3568 2601
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2640 4230 5386 1790
Size: 850 1590 1156 2573
Fragments arranged by size:
2573 1590 1156 850
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2288 3900 4858
Size: 416 53 83 399 24 871 1612 958
Cuts at: 4858 442
Size: 1753
Fragments arranged by size:
1753 1612 958 871 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2497 2897 4829 1239
Size: 1258 400 1932 2579
Fragments arranged by size:
2579 1932 1258 400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4294 5159 5457 4294
Size: 865 298 5006
Fragments arranged by size:
5006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2905 3960 493
Size: 711 1701 1055 2702
Fragments arranged by size:
2702 1701 1055 711
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2303 2439 3243 3381
Size: 53 83 186 936 770 136 804 138
Cuts at: 3381 3976 4358 4993 275
Size: 595 382 635 1451
Fragments arranged by size:
1451 936 804 770 635 595 382 186
138 136 83 53
BsaWI w'CCGG_w
Cuts at: 2615 4602 5000 5274 5421 5832 2615
Size: 1987 398 274 147 411 2952
Fragments arranged by size:
2952 1987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2870 2900 3184 3214 1178
Size: 30 165 30 1467 30 284 30 4133
Fragments arranged by size:
4133 1467 284 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 3112 1186
Size: 876 1050 4243
Fragments arranged by size:
4243 1050 876
BseYI C'CCAG_C
Cuts at: 2941 3104 3167 3266 5372 5868 2941
Size: 163 63 99 2106 496 3242
Fragments arranged by size:
3242 2106 496 163 99 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 3191 1163
Size: 573 402 1053 4141
Fragments arranged by size:
4141 1053 573 402
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2079 2211 2664 3619 4380
Size: 275 186 60 18 132 453 955 761
Cuts at: 4380 5408 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 955 761 453 275 186 132
60 18
BsiHKAI G_wGCw'C
Cuts at: 2640 2793 4230 4315 5386 2640
Size: 153 1437 85 1071 3423
Fragments arranged by size:
3423 1437 1071 153 85
BsmI GAATG_Cn'
Cuts at: 3680 3773 3680
Size: 93 6076
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2640 2793 3070 3104 3488 4230
Size: 399 447 403 153 277 34 384 742
Cuts at: 4230 4315 5386 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 447 403 399 384 277
153 85 34
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2868 3524 844
Size: 2024 656 3489
Fragments arranged by size:
3489 2024 656
BsrBI CCG'CTC
Cuts at: 1394 1823 2347 3181 4056 1394
Size: 429 524 834 875 3507
Fragments arranged by size:
3507 875 834 524 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2159 2615 2663 2982 4813 5000
Size: 110 268 81 456 48 319 1831 187
Cuts at: 5000 1700
Size: 2869
Fragments arranged by size:
2869 1831 456 319 268 187 110 81
48
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 2842 3453 1925
Size: 917 611 4641
Fragments arranged by size:
4641 917 611
BssSI C'ACGA_G
Cuts at: 4229 5241 4229
Size: 1012 5157
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6169
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 2296 2492 2296
Size: 196 5973
BstYI r'GATC_y
Cuts at: 1972 2170 2273 2596 2686 3499 3866 4251 4268
Size: 198 103 323 90 813 367 385 17
Cuts at: 4268 5720 5806 5818 6164 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 813 385 367 346 323 198
103 90 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5617
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2125 2951 487
Size: 683 56 899 826 3705
Fragments arranged by size:
3705 899 826 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 3685 4479 4499 3268
Size: 417 794 20 4938
Fragments arranged by size:
4938 794 417 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2570 5176 809
Size: 507 1254 2606 1802
Fragments arranged by size:
2606 1802 1254 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2076 2208
Size: 54 1425 50 275 18 228 18 132
Cuts at: 2208 2268 2661 2665 3616 4505 6134 8
Size: 60 393 4 951 889 1629 43
Fragments arranged by size:
1629 1425 951 889 393 275 228 132
60 54 50 43 18 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2076 2208 2661 3616 1537
Size: 275 246 18 132 453 955 4090
Fragments arranged by size:
4090 955 453 275 246 132 18
EarI CTCTTCn'nnn_
Cuts at: 1353 4099 1353
Size: 2746 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2409 2415 3001 4756 5130 5276
Size: 1064 138 820 6 586 1755 374 146
Cuts at: 5276 387
Size: 1280
Fragments arranged by size:
1755 1280 1064 820 586 374 146 138
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2497 2897 3135 3295 4232 4829
Size: 393 397 468 400 238 160 937 597
Cuts at: 4829 5616 5884 1239
Size: 787 268 1524
Fragments arranged by size:
1524 937 787 597 468 400 397 393
268 238 160
EcoO109I rG'GnC_Cy
Cuts at: 2191 2202 2777 3229 2191
Size: 11 575 452 5131
Fragments arranged by size:
5131 575 452 11
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 3473 2149
Size: 1324 4845
FseI GG_CCGG'CC
Cuts at: 2082 2667 2082
Size: 585 5584
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 2306 2442 3246 3321 3384 3426 5316 1678
Size: 628 136 804 75 63 42 1890 2531
Fragments arranged by size:
2531 1890 804 628 136 75 63 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3041 3073 3134 3166 4810
Size: 32 135 32 1469 32 61 32 1644
Cuts at: 4810 4842 4884 4916 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1644 1469 135 61 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3513 3759 669
Size: 988 281 1575 246 3079
Fragments arranged by size:
3079 1575 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KasI G'GCGC_C
Cuts at: 2302 2438 3242 3380 2302
Size: 136 804 138 5091
Fragments arranged by size:
5091 804 138 136
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2064 2880 5283 5467 1305
Size: 759 816 2403 184 2007
Fragments arranged by size:
2403 2007 816 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4347
Fragments arranged by size:
4347 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 2490 4127 4486 4645 518
Size: 648 1128 196 1637 359 159 2042
Fragments arranged by size:
2042 1637 1128 648 359 196 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2929 3285 3585 4262 5410 5655 1606
Size: 729 594 356 300 677 1148 245 2120
Fragments arranged by size:
2120 1148 729 677 594 356 300 245
NaeI GCC'GGC
Cuts at: 2080 2161 2665 2080
Size: 81 504 5584
Fragments arranged by size:
5584 504 81
NarI GG'CG_CC
Cuts at: 2303 2439 3243 3381 2303
Size: 136 804 138 5091
Fragments arranged by size:
5091 804 138 136
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5617
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NgoMIV G'CCGG_C
Cuts at: 2078 2159 2663 2078
Size: 81 504 5584
Fragments arranged by size:
5584 504 81
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3527 5072 1132
Size: 97 2298 1545 2229
Fragments arranged by size:
2298 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3062 1108
Size: 1954 4215
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3184 3216 4238 4270 5751 5783 3184
Size: 32 1022 32 1481 32 3570
Fragments arranged by size:
3570 1481 1022 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2777 2777
Size: 6169
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2448 2557 2655 2865 3521 830
Size: 1618 109 98 210 656 3478
Fragments arranged by size:
3478 1618 656 210 109 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 2929 2929
Size: 6169
RsrII CG'GwC_CG
Cuts at: 2917 2917
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 2444 2553 2651 2861 3517 4652 5333
Size: 219 1399 109 98 210 656 1135 681
Cuts at: 5333 5524 826
Size: 191 1471
Fragments arranged by size:
1471 1399 1135 681 656 219 210 191
109 98
SfoI GGC'GCC
Cuts at: 2304 2440 3244 3382 2304
Size: 136 804 138 5091
Fragments arranged by size:
5091 804 138 136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 2159 1700
Size: 459 5710
SmaI CCC'GGG
Cuts at: 1872 2432 3496 1872
Size: 560 1064 4545
Fragments arranged by size:
4545 1064 560
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4273 5174 5436 820
Size: 197 926 2330 901 262 1553
Fragments arranged by size:
2330 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 2649 2874 1517
Size: 1132 225 4812
Fragments arranged by size:
4812 1132 225
StyI C'CwwG_G
Cuts at: 513 1065 2103 3006 3367 3448 3608 513
Size: 552 1038 903 361 81 160 3074
Fragments arranged by size:
3074 1038 903 552 361 160 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2934 4196 4213 4366 4551 4996 325
Size: 1255 1354 1262 17 153 185 445 1498
Fragments arranged by size:
1498 1354 1262 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4415 6127 96
Size: 274 80 33 51 1443 2438 1712 138
Fragments arranged by size:
2438 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6129 6161 6129
Size: 32 6137
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 2683 3505 2683
Size: 822 5347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2296 3455 2296
Size: 1159 5010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 2430 3494 1870
Size: 560 1064 4545
Fragments arranged by size:
4545 1064 560
XmnI GAAnn'nnTTC
Cuts at: 4298 4298
Size: 6169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI