Restriction map for
pFN21AB7747
(Circular) (Six-base) MAPSORT of: pFN21AB7747.seq Check: 2668 from: 1 to: 6031
pFN21AB7747 6031 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3374 1937
Size: 1437 4594
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 4094 5478 5746 1632
Size: 728 1734 1384 268 1917
Fragments arranged by size:
1917 1734 1384 728 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 3226 3240 4930 5907 1203
Size: 2023 14 1690 977 1327
Fragments arranged by size:
2023 1690 1327 977 14
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 3121 4760 3121
Size: 1639 4392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6031
AlwNI CAG_nnn'CTG
Cuts at: 2276 2911 3774 5346 2276
Size: 635 863 1572 2961
Fragments arranged by size:
2961 1572 863 635
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 2488 3340 3566 3718 5827 1493
Size: 995 852 226 152 2109 1697
Fragments arranged by size:
2109 1697 995 852 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 3356 1870
Size: 73 1413 4545
Fragments arranged by size:
4545 1413 73
AvrII C'CTAG_G
Cuts at: 2957 2957
Size: 6031
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3352 4807 618
Size: 325 217 627 1565 1455 1842
Fragments arranged by size:
1842 1565 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3350 1391
Size: 1959 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 928
Size: 971 142 4918
Fragments arranged by size:
4918 971 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2282 2316 4222 4256 1903
Size: 34 345 34 1906 34 3678
Fragments arranged by size:
3678 1906 345 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2347 3923 5133 2347
Size: 1576 1210 3245
Fragments arranged by size:
3245 1576 1210
BclI T'GATC_A
Cuts at: 1548 2973 1548
Size: 1425 4606
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4642 136
Size: 107 122 71 631 605 337 2633 1525
Fragments arranged by size:
2633 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 2082 3448 2082
Size: 1366 4665
Bme1580I G_kGCm'C
Cuts at: 1790 4092 5248 1790
Size: 2302 1156 2573
Fragments arranged by size:
2573 2302 1156
BmgBI CAC'GTC
Cuts at: 1318 3227 3241 1318
Size: 1909 14 4108
Fragments arranged by size:
4108 1909 14
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2440 3762 4720
Size: 416 53 83 399 24 1023 1322 958
Cuts at: 4720 442
Size: 1753
Fragments arranged by size:
1753 1322 1023 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 4691 1239
Size: 1349 2103 2579
Fragments arranged by size:
2579 2103 1349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2156 2580 3031 4156 5021 5319 2129
Size: 27 424 451 1125 865 298 2841
Fragments arranged by size:
2841 1125 865 451 424 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2657 4694 882
Size: 617 1158 2037 2219
Fragments arranged by size:
2219 2037 1158 617
BsaAI yAC'GTr
Cuts at: 493 1204 3822 493
Size: 711 2618 2702
Fragments arranged by size:
2702 2618 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3161 3838 4220 4855
Size: 53 83 186 936 1628 677 382 635
Cuts at: 4855 275
Size: 1451
Fragments arranged by size:
1628 1451 936 677 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2510 4464 4862 5136 5283 5694 2510
Size: 1954 398 274 147 411 2847
Fragments arranged by size:
2847 1954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2741 2771 1178
Size: 30 165 30 1338 30 4438
Fragments arranged by size:
4438 1338 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2308 2441 1186
Size: 1122 133 4776
Fragments arranged by size:
4776 1122 133
BseYI C'CCAG_C
Cuts at: 2033 5234 5730 2033
Size: 3201 496 2334
Fragments arranged by size:
3201 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 3071 3294 1163
Size: 573 435 900 223 3900
Fragments arranged by size:
3900 900 573 435 223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3481 4242 5270 1540
Size: 275 186 1480 761 1028 2301
Fragments arranged by size:
2301 1480 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4092 4177 5248 4092
Size: 85 1071 4875
Fragments arranged by size:
4875 1071 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 2390 2390
Size: 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3350 4092 4177 5248 1391
Size: 399 1560 742 85 1071 2174
Fragments arranged by size:
2174 1560 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3386 844
Size: 2542 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2891 3918 1394
Size: 429 1068 1027 3507
Fragments arranged by size:
3507 1068 1027 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2248 4526 4700 65
Size: 1183 1000 2278 174 1396
Fragments arranged by size:
2278 1396 1183 1000 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2022 4675 4862 1700
Size: 110 212 2653 187 2869
Fragments arranged by size:
2869 2653 212 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6031
BssSI C'ACGA_G
Cuts at: 2781 4091 5103 2781
Size: 1310 1012 3709
Fragments arranged by size:
3709 1310 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6031
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstXI CCAn_nnnn'nTGG
Cuts at: 2824 2824
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 2813 3006 3361 3728 4113 4130 5582 5668
Size: 841 193 355 367 385 17 1452 86
Cuts at: 5668 5680 6026 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 841 385 367 355 346 193
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2349 2851 3142 513
Size: 552 939 345 502 291 3402
Fragments arranged by size:
3402 939 552 502 345 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 3547 4341 4361 3547
Size: 794 20 5217
Fragments arranged by size:
5217 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3124 3159 529
Size: 35 2560 35 3401
Fragments arranged by size:
3401 2560 35 35
DraI TTT'AAA
Cuts at: 2950 3335 2950
Size: 385 5646
DraIII CAC_nnn'GTG
Cuts at: 3272 3272
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3145 3478 4367
Size: 54 1425 50 275 18 1315 333 889
Cuts at: 4367 5996 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1315 889 333 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3478 1537
Size: 275 1666 4090
Fragments arranged by size:
4090 1666 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2660 3961 1353
Size: 755 552 1301 3423
Fragments arranged by size:
3423 1301 755 552
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4618 4992 5138 387
Size: 1064 138 3029 374 146 1280
Fragments arranged by size:
3029 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2588 4094 4691 5478 5746 1239
Size: 393 728 228 1506 597 787 268 1524
Fragments arranged by size:
1524 1506 787 728 597 393 268 228
EcoO109I rG'GnC_Cy
Cuts at: 2954 3097 2954
Size: 143 5888
EcoRI G'AATT_C
Cuts at: 2488 3340 3718 2488
Size: 852 378 4801
Fragments arranged by size:
4801 852 378
EcoRV GAT'ATC
Cuts at: 2401 2401
Size: 6031
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 2134 5178 1678
Size: 456 3044 2531
Fragments arranged by size:
3044 2531 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 2805 2837 2980
Size: 32 135 32 854 32 347 32 143
Cuts at: 2980 2998 3012 3030 3107 3139 4672 4704 4746
Size: 18 14 18 77 32 1533 32 42
Cuts at: 4746 4778 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 854 347 143 135 77 42
32 32 32 32 32 32 32 18
18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2807 748
Size: 2059 3972
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2597 2841 5145 5329 1305
Size: 1292 244 2304 184 2007
Fragments arranged by size:
2304 2007 1292 244 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4209
Fragments arranged by size:
4209 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2241 3192 3989 4348 4507 518
Size: 648 1075 951 797 359 159 2042
Fragments arranged by size:
2042 1075 951 797 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2273 2537 3447 4124 5272 5517 1606
Size: 431 236 264 910 677 1148 245 2120
Fragments arranged by size:
2120 1148 910 677 431 264 245 236
NaeI GCC'GGC
Cuts at: 2024 2024
Size: 6031
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4540
Fragments arranged by size:
4540 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NgoMIV G'CCGG_C
Cuts at: 2022 2022
Size: 6031
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 2457 3389 4934 1132
Size: 97 1228 932 1545 2229
Fragments arranged by size:
2229 1545 1228 932 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6031
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6031
PfoI T'CCnGG_A
Cuts at: 2625 2980 2625
Size: 355 5676
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2954 2954
Size: 6031
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PstI C_TGCA'G
Cuts at: 830 3383 830
Size: 2553 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
PvuII CAG'CTG
Cuts at: 2273 2537 2273
Size: 264 5767
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 3379 4514 5195 5386 826
Size: 219 2334 1135 681 191 1471
Fragments arranged by size:
2334 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2135 2595 3046 4135 5036
Size: 197 926 165 27 460 451 1089 901
Cuts at: 5036 5298 820
Size: 262 1553
Fragments arranged by size:
1553 1089 926 901 460 451 262 197
165 27
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 3284 1517
Size: 1767 4264
StyI C'CwwG_G
Cuts at: 513 1065 2004 2189 2214 2957 3470 513
Size: 552 939 185 25 743 513 3074
Fragments arranged by size:
3074 939 743 552 513 185 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3284 4058 4075 4228 4413 4858 325
Size: 1255 1704 774 17 153 185 445 1498
Fragments arranged by size:
1704 1498 1255 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 3277 4277
Size: 274 80 33 51 1443 696 604 1000
Cuts at: 4277 5989 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1000 696 604 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5991 6023 5991
Size: 32 5999
Tth111I GACn'n_nGTC
Cuts at: 1316 2045 1316
Size: 729 5302
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 3173 4160 3173
Size: 987 5044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI