Restriction map for
pFN21AB7746
(Circular) (Six-base) MAPSORT of: pFN21AB7746.seq Check: 3776 from: 1 to: 6166
pFN21AB7746 6166 bp
With 182 enzymes: SgfI *
March 28, 2012 12:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3976 278
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2136 3509 1937
Size: 199 1373 4594
Fragments arranged by size:
4594 1373 199
Acc65I G'GTAC_C
Cuts at: 3487 3487
Size: 6166
AclI AA'CG_TT
Cuts at: 1563 2363 4293 4666 1563
Size: 800 1930 373 3063
Fragments arranged by size:
3063 1930 800 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2679 3255 3320 4229 5613 5881 1632
Size: 1047 576 65 909 1384 268 1917
Fragments arranged by size:
1917 1384 1047 909 576 268 65
AflII C'TTAA_G
Cuts at: 820 1017 2405 3311 820
Size: 197 1388 906 3675
Fragments arranged by size:
3675 1388 906 197
AflIII A'CryG_T
Cuts at: 1203 3081 5065 6042 1203
Size: 1878 1984 977 1327
Fragments arranged by size:
1984 1878 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 2626 4895 2626
Size: 2269 3897
AleI CACnn'nnGTG
Cuts at: 1166 3107 1166
Size: 1941 4225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6166
AlwNI CAG_nnn'CTG
Cuts at: 2830 3909 5481 2830
Size: 1079 1572 3515
Fragments arranged by size:
3515 1572 1079
ApaLI G'TGCA_C
Cuts at: 4223 5379 4223
Size: 1156 5010
ApoI r'AATT_y
Cuts at: 1493 3265 3475 3701 3853 5962 1493
Size: 1772 210 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 210 152
AseI AT'TA_AT
Cuts at: 160 1032 2886 4720 160
Size: 872 1854 1834 1606
Fragments arranged by size:
1854 1834 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 3491 1870
Size: 73 1548 4545
Fragments arranged by size:
4545 1548 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2596 2629 2596
Size: 33 6133
BamHI G'GATC_C
Cuts at: 3496 3863 3496
Size: 367 5799
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3101 3487 4942 618
Size: 325 217 627 1314 386 1455 1842
Fragments arranged by size:
1842 1455 1314 627 386 325 217
BanII G_rGCy'C
Cuts at: 1391 2801 3485 1391
Size: 1410 684 4072
Fragments arranged by size:
4072 1410 684
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5195
BbvCI CC'TCA_GC
Cuts at: 2021 2021
Size: 6166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4357 4391 1903
Size: 34 2420 34 3678
Fragments arranged by size:
3678 2420 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2597 4058 5268 2597
Size: 1461 1210 3495
Fragments arranged by size:
3495 1461 1210
BclI T'GATC_A
Cuts at: 1548 3111 1548
Size: 1563 4603
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2038 3437 4777
Size: 107 122 71 631 605 366 1399 1340
Cuts at: 4777 136
Size: 1525
Fragments arranged by size:
1525 1399 1340 631 605 366 122 107
71
BglII A'GATC_T
Cuts at: 6161 6161
Size: 6166
BlpI GC'TnA_GC
Cuts at: 3583 3583
Size: 6166
Bme1580I G_kGCm'C
Cuts at: 1790 3023 3104 4227 5383 1790
Size: 1233 81 1123 1156 2573
Fragments arranged by size:
2573 1233 1156 1123 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3107 3897 4855
Size: 416 53 83 399 24 1690 790 958
Cuts at: 4855 442
Size: 1753
Fragments arranged by size:
1753 1690 958 790 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2565 4826 1239
Size: 950 376 2261 2579
Fragments arranged by size:
2579 2261 950 376
Bpu10I CC'TnA_GC
Cuts at: 2021 2802 2021
Size: 781 5385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4291 5156 5454 4291
Size: 865 298 5003
Fragments arranged by size:
5003 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 3155 4829 882
Size: 617 651 1005 1674 2219
Fragments arranged by size:
2219 1674 1005 651 617
BsaAI yAC'GTr
Cuts at: 493 1204 3957 493
Size: 711 2753 2702
Fragments arranged by size:
2753 2702 711
BsaBI GATnn'nnATC
Cuts at: 2685 6160 2685
Size: 3475 2691
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3973 4355 4990 275
Size: 53 83 186 936 2440 382 635 1451
Fragments arranged by size:
2440 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4599 4997 5271 5418 5829 4599
Size: 398 274 147 411 4936
Fragments arranged by size:
4936 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2707 2737 1178
Size: 30 165 30 1304 30 4607
Fragments arranged by size:
4607 1304 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2715 1186
Size: 1529 4637
BseYI C'CCAG_C
Cuts at: 2925 5369 5865 2925
Size: 2444 496 3226
Fragments arranged by size:
3226 2444 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5593
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3616 4377 5405 1540
Size: 275 186 1615 761 1028 2301
Fragments arranged by size:
2301 1615 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3075 4227 4312 5383 3075
Size: 1152 85 1071 3858
Fragments arranged by size:
3858 1152 1071 85
BsmI GAATG_Cn'
Cuts at: 3677 3770 3677
Size: 93 6073
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 3442 2003
Size: 1439 4727
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2801 3023 3075 3104 3485 4227 4312
Size: 399 1011 222 52 29 381 742 85
Cuts at: 4312 5383 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1011 742 399 381 222 85
52 29
BspEI T'CCGG_A
Cuts at: 5829 5829
Size: 6166
BspHI T'CATG_A
Cuts at: 2706 4055 5785 2706
Size: 1349 1730 3087
Fragments arranged by size:
3087 1730 1349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2507 3521 844
Size: 1663 1014 3489
Fragments arranged by size:
3489 1663 1014
BsrBI CCG'CTC
Cuts at: 1394 1823 4053 1394
Size: 429 2230 3507
Fragments arranged by size:
3507 2230 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2246 4661 4835 65
Size: 1183 998 2415 174 1396
Fragments arranged by size:
2415 1396 1183 998 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2159 4810 4997 1700
Size: 110 227 122 2651 187 2869
Fragments arranged by size:
2869 2651 227 187 122 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6166
BssSI C'ACGA_G
Cuts at: 4226 5238 4226
Size: 1012 5154
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2498 794
Size: 1704 4462
BstBI TT'CG_AA
Cuts at: 4970 4970
Size: 6166
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6166
BstXI CCAn_nnnn'nTGG
Cuts at: 2653 2751 2653
Size: 98 6068
BstYI r'GATC_y
Cuts at: 1972 3387 3496 3863 4248 4265 5717 5803 5815
Size: 1415 109 367 385 17 1452 86 12
Cuts at: 5815 6161 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1415 385 367 346 109 86
17 12
BstZ17I GTA'TAC
Cuts at: 2137 2137
Size: 6166
BtgI C'CryG_G
Cuts at: 513 1065 2004 2535 2895 3105 513
Size: 552 939 531 360 210 3574
Fragments arranged by size:
3574 939 552 531 360 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5427
Fragments arranged by size:
5427 683 56
BtsI GCAGTG_nn'
Cuts at: 3009 3682 4476 4496 3009
Size: 673 794 20 4679
Fragments arranged by size:
4679 794 673 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 3470 3470
Size: 6166
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5173 809
Size: 507 3857 1802
Fragments arranged by size:
3857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 2538 3613
Size: 54 1425 50 275 18 331 377 1075
Cuts at: 3613 4502 6131 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1075 889 377 331 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3613 1537
Size: 275 1801 4090
Fragments arranged by size:
4090 1801 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2961 3126 3335 3378 3440 4096 1353
Size: 1608 165 209 43 62 656 3423
Fragments arranged by size:
3423 1608 656 209 165 62 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4753 5127 5273 387
Size: 1064 138 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2565 2679 3255 3320 4229 4826
Size: 393 557 376 114 576 65 909 597
Cuts at: 4826 5613 5881 1239
Size: 787 268 1524
Fragments arranged by size:
1524 909 787 597 576 557 393 376
268 114 65
EcoO109I rG'GnC_Cy
Cuts at: 2121 3331 2121
Size: 1210 4956
EcoRI G'AATT_C
Cuts at: 3475 3853 3475
Size: 378 5788
EcoRV GAT'ATC
Cuts at: 2341 2452 2683 2341
Size: 111 231 5824
Fragments arranged by size:
5824 231 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3364 3364
Size: 6166
FspI TGC'GCA
Cuts at: 4672 4672
Size: 6166
HaeII r_GCGC'y
Cuts at: 1678 5313 1678
Size: 3635 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 2738 2770 4807
Size: 32 135 32 1040 32 94 32 2037
Cuts at: 4807 4839 4881 4913 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2037 1040 135 94 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2367 2522 3510 3756 669
Size: 988 281 429 155 988 246 3079
Fragments arranged by size:
3079 988 988 429 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6166
HpaI GTT'AAC
Cuts at: 2367 3756 2367
Size: 1389 4777
KpnI G_GTAC'C
Cuts at: 3491 3491
Size: 6166
MfeI C'AATT_G
Cuts at: 3765 3765
Size: 6166
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 3299 5280 5464 1305
Size: 729 1265 1981 184 2007
Fragments arranged by size:
2007 1981 1265 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2540 10
Size: 54 1425 343 708 3636
Fragments arranged by size:
3636 1425 708 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 2900 3107 4124 4483 4642 518
Size: 648 1485 249 207 1017 359 159 2042
Fragments arranged by size:
2042 1485 1017 648 359 249 207 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2044 2932 3582 4259 5407 5652 1606
Size: 414 24 888 650 677 1148 245 2120
Fragments arranged by size:
2120 1148 888 677 650 414 245 24
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 6166
NcoI C'CATG_G
Cuts at: 513 1065 2004 2535 2895 3105 513
Size: 552 939 531 360 210 3574
Fragments arranged by size:
3574 939 552 531 360 210
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6166
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 6166
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6166
NotI GC'GGCC_GC
Cuts at: 3613 3613
Size: 6166
NspI r_CATG'y
Cuts at: 1132 1229 2492 3524 5069 1132
Size: 97 1263 1032 1545 2229
Fragments arranged by size:
2229 1545 1263 1032 97
PacI TTA_AT'TAA
Cuts at: 2886 2886
Size: 6166
PasI CC'CwG_GG
Cuts at: 1348 2632 1348
Size: 1284 4882
PciI A'CATG_T
Cuts at: 5065 5065
Size: 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3293 1108
Size: 2185 3981
PfoI T'CCnGG_A
Cuts at: 2205 3423 2205
Size: 1218 4948
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2121 3331 2121
Size: 1210 4956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6166
PsiI TTA'TAA
Cuts at: 3736 3736
Size: 6166
PstI C_TGCA'G
Cuts at: 830 2835 3518 830
Size: 2005 683 3478
Fragments arranged by size:
3478 2005 683
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2020 2044 2020
Size: 24 6142
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 2315 4414 6126 2315
Size: 2099 1712 2355
Fragments arranged by size:
2355 2099 1712
SexAI A'CCwGG_T
Cuts at: 3250 3250
Size: 6166
SfcI C'TryA_G
Cuts at: 826 1045 2831 3514 4649 5330 5521 826
Size: 219 1786 683 1135 681 191 1471
Fragments arranged by size:
1786 1471 1135 683 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 3493 1872
Size: 1621 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 3311 4270 5171 5433 820
Size: 197 926 462 906 959 901 262 1553
Fragments arranged by size:
1553 959 926 906 901 462 262 197
SnaBI TAC'GTA
Cuts at: 493 3957 493
Size: 3464 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 3524 1132
Size: 2392 3774
SspI AAT'ATT
Cuts at: 5 52 2267 2391 2467 3197 4090 5
Size: 47 2215 124 76 730 893 2081
Fragments arranged by size:
2215 2081 893 730 124 76 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6166
StyI C'CwwG_G
Cuts at: 513 1065 2004 2535 2895 3105 3605 513
Size: 552 939 531 360 210 500 3074
Fragments arranged by size:
3074 939 552 531 500 360 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2853 2996 4193 4210 4363 4548 4993
Size: 1255 1273 143 1197 17 153 185 445
Cuts at: 4993 325
Size: 1498
Fragments arranged by size:
1498 1273 1255 1197 445 185 153 143
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2313 4412 6124
Size: 274 80 33 51 1443 336 2099 1712
Cuts at: 6124 96
Size: 138
Fragments arranged by size:
2099 1712 1443 336 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6126 6158 6126
Size: 32 6134
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6166
XbaI T'CTAG_A
Cuts at: 3502 3502
Size: 6166
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6166
XmaI C'CCGG_G
Cuts at: 1870 3491 1870
Size: 1621 4545
XmnI GAAnn'nnTTC
Cuts at: 2421 2589 4295 2421
Size: 168 1706 4292
Fragments arranged by size:
4292 1706 168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3974 276
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI Bsu36I DraIII EcoICRI EcoNI FseI FspAI
KasI NarI NruI NsiI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI