Restriction map for
pFN21AB7743
(Circular) (Six-base) MAPSORT of: pFN21AB7743.seq Check: 9351 from: 1 to: 6016
pFN21AB7743 6016 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2457 3359 1937
Size: 520 902 4594
Fragments arranged by size:
4594 902 520
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2504 4079 5463 5731 1632
Size: 872 1575 1384 268 1917
Fragments arranged by size:
1917 1575 1384 872 268
AfeI AGC'GCT
Cuts at: 2417 2417
Size: 6016
AflII C'TTAA_G
Cuts at: 820 1017 2861 820
Size: 197 1844 3975
Fragments arranged by size:
3975 1844 197
AflIII A'CryG_T
Cuts at: 1203 2744 4915 5892 1203
Size: 1541 2171 977 1327
Fragments arranged by size:
2171 1541 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 3124 4745 3124
Size: 1621 4395
AleI CACnn'nnGTG
Cuts at: 1166 2591 2993 1166
Size: 1425 402 4189
Fragments arranged by size:
4189 1425 402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AlwNI CAG_nnn'CTG
Cuts at: 2305 2575 2662 2731 2774 3759 5331 2305
Size: 270 87 69 43 985 1572 2990
Fragments arranged by size:
2990 1572 985 270 87 69 43
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 6016
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 3325 3551 3703 5812 1493
Size: 1832 226 152 2109 1697
Fragments arranged by size:
2109 1832 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2328 2368 3248 3341 1870
Size: 73 120 265 40 880 93 4545
Fragments arranged by size:
4545 880 265 120 93 73 40
BamHI G'GATC_C
Cuts at: 3346 3713 3346
Size: 367 5649
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 2215 3337 4792 618
Size: 325 217 627 374 54 1122 1455 1842
Fragments arranged by size:
1842 1455 1122 627 374 325 217 54
BanII G_rGCy'C
Cuts at: 1391 2023 3106 3335 1391
Size: 632 1083 229 4072
Fragments arranged by size:
4072 1083 632 229
BbeI G_GCGC'C
Cuts at: 2165 2219 2165
Size: 54 5962
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2939 928
Size: 971 1040 4005
Fragments arranged by size:
4005 1040 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3908 5118 3908
Size: 1210 4806
BclI T'GATC_A
Cuts at: 1548 2796 1548
Size: 1248 4768
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2141 4627 136
Size: 107 122 71 631 605 469 2486 1525
Fragments arranged by size:
2486 1525 631 605 469 122 107 71
BglII A'GATC_T
Cuts at: 2853 6011 2853
Size: 3158 2858
BlpI GC'TnA_GC
Cuts at: 2432 3433 2432
Size: 1001 5015
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2900 4077 5233 1790
Size: 233 877 1177 1156 2573
Fragments arranged by size:
2573 1177 1156 877 233
BmgBI CAC'GTC
Cuts at: 1318 2745 1318
Size: 1427 4589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2226 2476 3747
Size: 416 53 83 399 24 809 250 1271
Cuts at: 3747 4705 442
Size: 958 1753
Fragments arranged by size:
1753 1271 958 809 416 399 250 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 3193
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2444 3083 3176 4676 1239
Size: 833 372 639 93 1500 2579
Fragments arranged by size:
2579 1500 833 639 372 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2633 4141 5006 5304 2546
Size: 87 1508 865 298 3258
Fragments arranged by size:
3258 1508 865 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2384 2807 2900 3264 4679 882
Size: 617 885 423 93 364 1415 2219
Fragments arranged by size:
2219 1415 885 617 423 364 93
BsaAI yAC'GTr
Cuts at: 493 1204 2740 3807 493
Size: 711 1536 1067 2702
Fragments arranged by size:
2702 1536 1067 711
BsaBI GATnn'nnATC
Cuts at: 2105 2678 6010 2105
Size: 573 3332 2111
Fragments arranged by size:
3332 2111 573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2162 2216 2598
Size: 53 83 186 936 594 35 54 382
Cuts at: 2598 3823 4205 4840 275
Size: 1225 382 635 1451
Fragments arranged by size:
1451 1225 936 635 594 382 382 186
83 54 53 35
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5791
Fragments arranged by size:
5791 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 2918 2975 2978 3176 3209 3266 1186
Size: 897 835 57 3 198 33 57 3936
Fragments arranged by size:
3936 897 835 198 57 57 33 3
BseYI C'CCAG_C
Cuts at: 2575 2817 3041 5219 5715 2575
Size: 242 224 2178 496 2876
Fragments arranged by size:
2876 2178 496 242 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2510 1163
Size: 573 774 4669
Fragments arranged by size:
4669 774 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2257 2883 3144 3466 4227 5255
Size: 275 186 256 626 261 322 761 1028
Cuts at: 5255 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 626 322 275 261 256
186
BsiHKAI G_wGCw'C
Cuts at: 4077 4162 5233 4077
Size: 85 1071 4860
Fragments arranged by size:
4860 1071 85
BsmI GAATG_Cn'
Cuts at: 3527 3620 3527
Size: 93 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2900 3106 3335 4077 4162 5233
Size: 399 233 877 206 229 742 85 1071
Cuts at: 5233 1391
Size: 2174
Fragments arranged by size:
2174 1071 877 742 399 233 229 206
85
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 3371 844
Size: 1355 1172 3489
Fragments arranged by size:
3489 1355 1172
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 2763 4511 4685 65
Size: 1183 1293 222 1748 174 1396
Fragments arranged by size:
1748 1396 1293 1183 222 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 2069 2140 2150 2171 2263 4660
Size: 110 234 25 71 10 21 92 2397
Cuts at: 4660 4847 1700
Size: 187 2869
Fragments arranged by size:
2869 2397 234 187 110 92 71 25
21 10
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6016
BssHII G'CGCG_C
Cuts at: 1925 2040 2073 1925
Size: 115 33 5868
Fragments arranged by size:
5868 115 33
BssSI C'ACGA_G
Cuts at: 3221 4076 5088 3221
Size: 855 1012 4149
Fragments arranged by size:
4149 1012 855
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2774 794
Size: 1980 4036
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6016
BstYI r'GATC_y
Cuts at: 1972 2853 3037 3346 3713 4098 4115 5567 5653
Size: 881 184 309 367 385 17 1452 86
Cuts at: 5653 5665 6011 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 881 385 367 346 309 184
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2901 3192 2901
Size: 291 5725
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 2240 2829 3108 513
Size: 552 939 153 83 589 279 3421
Fragments arranged by size:
3421 939 589 552 279 153 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 2323 3532 4326 4346 2323
Size: 1209 794 20 3993
Fragments arranged by size:
3993 1209 794 20
ClaI AT'CG_AT
Cuts at: 1554 2883 1554
Size: 1329 4687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3320 3320
Size: 6016
DraIII CAC_nnn'GTG
Cuts at: 2234 2234
Size: 6016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2453 5023 809
Size: 507 1137 2570 1802
Fragments arranged by size:
2570 1802 1137 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2254 2307
Size: 54 1425 50 275 18 183 241 53
Cuts at: 2307 3141 3463 4352 5981 8
Size: 834 322 889 1629 43
Fragments arranged by size:
1629 1425 889 834 322 275 241 183
54 53 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2254 3141 3463 1537
Size: 275 442 887 322 4090
Fragments arranged by size:
4090 887 442 322 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2204 2842 3281 3946 1353
Size: 851 638 439 665 3423
Fragments arranged by size:
3423 851 665 638 439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2104 2619 4603 4977 5123 387
Size: 1064 138 515 515 1984 374 146 1280
Fragments arranged by size:
1984 1280 1064 515 515 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2444 2504 3083 3176 4079 4676
Size: 393 440 372 60 579 93 903 597
Cuts at: 4676 5463 5731 1239
Size: 787 268 1524
Fragments arranged by size:
1524 903 787 597 579 440 393 372
268 93 60
EcoNI CCTnn'n_nnAGG
Cuts at: 3191 3251 3191
Size: 60 5956
EcoO109I rG'GnC_Cy
Cuts at: 2019 2020 2056 2629 2752 3226 3295 2019
Size: 1 36 573 123 474 69 4740
Fragments arranged by size:
4740 573 474 123 69 36 1
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2051 2136 2165 2219 2419 2731 5163 1678
Size: 373 85 29 54 200 312 2432 2531
Fragments arranged by size:
2531 2432 373 312 200 85 54 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3110 3142 4657 4689 4731
Size: 32 135 32 1538 32 1515 32 42
Cuts at: 4731 4763 1373
Size: 32 2626
Fragments arranged by size:
2626 1538 1515 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 2622 3360 3606 669
Size: 988 281 520 164 738 246 3079
Fragments arranged by size:
3079 988 738 520 281 246 164
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6016
HpaI GTT'AAC
Cuts at: 3606 3606
Size: 6016
KasI G'GCGC_C
Cuts at: 2161 2215 2161
Size: 54 5962
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 3615 3615
Size: 6016
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2659 5130 5314 1305
Size: 1354 2471 184 2007
Fragments arranged by size:
2471 2007 1354 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4194
Fragments arranged by size:
4194 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 2993 3974 4333 4492 518
Size: 648 1425 402 981 359 159 2042
Fragments arranged by size:
2042 1425 981 648 402 359 159
MspA1I CmG'CkG
Cuts at: 1606 2159 2242 2641 2662 2695 3076 3133 3432
Size: 553 83 399 21 33 381 57 299
Cuts at: 3432 4109 5257 5502 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 553 399 381 299 245
83 57 33 21
NaeI GCC'GGC
Cuts at: 2046 2071 2142 2173 2046
Size: 25 71 31 5889
Fragments arranged by size:
5889 71 31 25
NarI GG'CG_CC
Cuts at: 2162 2216 2162
Size: 54 5962
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4525
Fragments arranged by size:
4525 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NgoMIV G'CCGG_C
Cuts at: 2044 2069 2140 2171 2044
Size: 25 71 31 5889
Fragments arranged by size:
5889 71 31 25
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 3164 3374 4919 1132
Size: 97 1935 210 1545 2229
Fragments arranged by size:
2229 1935 1545 210 97
PasI CC'CwG_GG
Cuts at: 1348 2927 1348
Size: 1579 4437
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2575 2731 2830 1108
Size: 1467 156 99 4294
Fragments arranged by size:
4294 1467 156 99
PfoI T'CCnGG_A
Cuts at: 2347 2421 3083 3153 2347
Size: 74 662 70 5210
Fragments arranged by size:
5210 662 74 70
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 5598 5630 4085
Size: 32 1481 32 4471
Fragments arranged by size:
4471 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2629 2752 3295 2629
Size: 123 543 5350
Fragments arranged by size:
5350 543 123
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 6016
PspXI vC'TCGA_Gb
Cuts at: 3248 3248
Size: 6016
PstI C_TGCA'G
Cuts at: 830 2646 2779 3368 830
Size: 1816 133 589 3478
Fragments arranged by size:
3478 1816 589 133
PvuI CG_AT'CG
Cuts at: 2001 2883 2001
Size: 882 5134
PvuII CAG'CTG
Cuts at: 2641 2662 2695 2641
Size: 21 33 5962
Fragments arranged by size:
5962 33 21
SacII CC_GC'GG
Cuts at: 2160 2243 2160
Size: 83 5933
SalI G'TCGA_C
Cuts at: 1936 2456 3358 1936
Size: 520 902 4594
Fragments arranged by size:
4594 902 520
SapI GCTCTTCn'nnn_
Cuts at: 2204 2204
Size: 6016
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 2642 2775 3364 4499 5180 5371 826
Size: 219 1597 133 589 1135 681 191 1471
Fragments arranged by size:
1597 1471 1135 681 589 219 191 133
SfoI GGC'GCC
Cuts at: 2163 2217 2163
Size: 54 5962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 2044 2263 1700
Size: 344 219 5453
Fragments arranged by size:
5453 344 219
SmaI CCC'GGG
Cuts at: 1872 2370 3343 1872
Size: 498 973 4545
Fragments arranged by size:
4545 973 498
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2525 2612 2861 3248 4120 5021
Size: 197 926 582 87 249 387 872 901
Cuts at: 5021 5283 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 872 582 387 262 249
197 87
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6016
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 3455 513
Size: 552 939 6 1445 3074
Fragments arranged by size:
3074 1445 939 552 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 4043 4060 4213 4398 4843 325
Size: 1255 665 1798 17 153 185 445 1498
Fragments arranged by size:
1798 1498 1255 665 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2684 4262 5974
Size: 274 80 33 51 1443 707 1578 1712
Cuts at: 5974 96
Size: 138
Fragments arranged by size:
1712 1578 1443 707 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 3022 3054 5976 6008 2343
Size: 32 647 32 2922 32 2351
Fragments arranged by size:
2922 2351 647 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2450 1316
Size: 1134 4882
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2419
Size: 6016
XhoI C'TCGA_G
Cuts at: 1943 3248 1943
Size: 1305 4711
XmaI C'CCGG_G
Cuts at: 1870 2368 3341 1870
Size: 498 973 4545
Fragments arranged by size:
4545 973 498
XmnI GAAnn'nnTTC
Cuts at: 4145 4145
Size: 6016
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PsrI RsrII SacI SanDI
SexAI SfiI SrfI SwaI