Restriction map for
pFN21AB7741
(Circular) (Six-base) MAPSORT of: pFN21AB7741.seq Check: 7183 from: 1 to: 6013
pFN21AB7741 6013 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2098 4076 5460 5728 1632
Size: 466 1978 1384 268 1917
Fragments arranged by size:
1978 1917 1384 466 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 4912 5889 1203
Size: 3709 977 1327
Fragments arranged by size:
3709 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AlwNI CAG_nnn'CTG
Cuts at: 3756 5328 3756
Size: 1572 4441
ApaLI G'TGCA_C
Cuts at: 2472 4070 5226 2472
Size: 1598 1156 3259
Fragments arranged by size:
3259 1598 1156
ApoI r'AATT_y
Cuts at: 1493 2727 3322 3548 3700 5809 1493
Size: 1234 595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1234 595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 3338 1870
Size: 73 1395 4545
Fragments arranged by size:
4545 1395 73
BamHI G'GATC_C
Cuts at: 3343 3710 3343
Size: 367 5646
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3334 4789 618
Size: 325 217 627 1547 1455 1842
Fragments arranged by size:
1842 1547 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2374 2998 3305 3332 1391
Size: 983 624 307 27 4072
Fragments arranged by size:
4072 983 624 307 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2326 3073 3157 928
Size: 971 427 747 84 3784
Fragments arranged by size:
3784 971 747 427 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BfrBI ATG'CAT
Cuts at: 2659 2659
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4624 136
Size: 107 122 71 631 605 2952 1525
Fragments arranged by size:
2952 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2825 6008 2825
Size: 3183 2830
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2476 4074 5230 1790
Size: 686 1598 1156 2573
Fragments arranged by size:
2573 1598 1156 686
BmgBI CAC'GTC
Cuts at: 1318 2973 1318
Size: 1655 4358
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2457 2709 3048
Size: 416 53 83 399 24 1040 252 339
Cuts at: 3048 3744 4702 442
Size: 696 958 1753
Fragments arranged by size:
1753 1040 958 696 416 399 339 252
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2945
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2316 2685 2769 2858 3024 3192 4673 1239
Size: 1077 369 84 89 166 168 1481 2579
Fragments arranged by size:
2579 1481 1077 369 168 166 89 84
Bpu10I CC'TnA_GC
Cuts at: 3299 3299
Size: 6013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 5003 5301 4138
Size: 865 298 4850
Fragments arranged by size:
4850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2497 4676 882
Size: 617 998 2179 2219
Fragments arranged by size:
2219 2179 998 617
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 6007 6007
Size: 6013
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2420 3820 4202 4837
Size: 53 83 186 936 887 1400 382 635
Cuts at: 4837 275
Size: 1451
Fragments arranged by size:
1451 1400 936 887 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5788
Fragments arranged by size:
5788 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 1186
Size: 880 5133
BseYI C'CCAG_C
Cuts at: 5216 5712 5216
Size: 496 5517
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3157 1163
Size: 573 1421 4019
Fragments arranged by size:
4019 1421 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2133 3463 4224 5252 1540
Size: 275 186 132 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186 132
BsiHKAI G_wGCw'C
Cuts at: 2374 2476 2998 4074 4159 5230 2374
Size: 102 522 1076 85 1071 3157
Fragments arranged by size:
3157 1076 1071 522 102 85
BsmI GAATG_Cn'
Cuts at: 2519 3524 3617 2519
Size: 1005 93 4915
Fragments arranged by size:
4915 1005 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2411 2411
Size: 6013
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2374 2476 2998 3305 3332 4074 4159
Size: 399 584 102 522 307 27 742 85
Cuts at: 4159 5230 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 584 522 399 307 102
85 27
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3238 3902 5632 3238
Size: 664 1730 3619
Fragments arranged by size:
3619 1730 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 3368 844
Size: 1945 579 3489
Fragments arranged by size:
3489 1945 579
BsrBI CCG'CTC
Cuts at: 1394 1823 3900 1394
Size: 429 2077 3507
Fragments arranged by size:
3507 2077 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4508 4682 65
Size: 1183 3260 174 1396
Fragments arranged by size:
3260 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4844 1700
Size: 110 2847 187 2869
Fragments arranged by size:
2869 2847 187 110
BsrGI T'GTAC_A
Cuts at: 96 3107 96
Size: 3011 3002
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6013
BssSI C'ACGA_G
Cuts at: 4073 5085 4073
Size: 1012 5001
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2523 794
Size: 1729 4284
BstBI TT'CG_AA
Cuts at: 2641 3064 4817 2641
Size: 423 1753 3837
Fragments arranged by size:
3837 1753 423
BstEII G'GTnAC_C
Cuts at: 1184 2801 1184
Size: 1617 4396
BstXI CCAn_nnnn'nTGG
Cuts at: 3275 3275
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2825 3343 3710 4095 4112 5564 5650 5662
Size: 853 518 367 385 17 1452 86 12
Cuts at: 5662 6008 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 853 518 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4522
Fragments arranged by size:
4522 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 4323 4343 3529
Size: 794 20 5199
Fragments arranged by size:
5199 794 20
ClaI AT'CG_AT
Cuts at: 1554 2706 1554
Size: 1152 4861
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5978
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5020 809
Size: 507 3704 1802
Fragments arranged by size:
3704 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3255 3460 4349
Size: 54 1425 50 275 18 1425 205 889
Cuts at: 4349 5978 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 275 205 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3943 1353
Size: 2590 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2372 2996 2372
Size: 624 5389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2316 2685 2769 2858 3024 3192
Size: 393 466 218 369 84 89 166 168
Cuts at: 3192 4076 4673 5460 5728 1239
Size: 884 597 787 268 1524
Fragments arranged by size:
1524 884 787 597 466 393 369 268
218 168 166 89 84
EcoO109I rG'GnC_Cy
Cuts at: 2589 2589
Size: 6013
EcoRI G'AATT_C
Cuts at: 3322 3700 3322
Size: 378 5635
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4654 4686 4728 4760 1373
Size: 32 135 32 3082 32 42 32 2626
Fragments arranged by size:
3082 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3357 3603 669
Size: 988 281 1419 246 3079
Fragments arranged by size:
3079 1419 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 3612 3612
Size: 6013
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2113 5127 5311 1305
Size: 808 3014 184 2007
Fragments arranged by size:
3014 2007 808 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2311 2901 3103 3971 4330 4489 518
Size: 648 1145 590 202 868 359 159 2042
Fragments arranged by size:
2042 1145 868 648 590 359 202 159
MspA1I CmG'CkG
Cuts at: 1606 3429 4106 5254 5499 1606
Size: 1823 677 1148 245 2120
Fragments arranged by size:
2120 1823 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4522
Fragments arranged by size:
4522 939 552
NdeI CA'TA_TG
Cuts at: 387 3100 387
Size: 2713 3300
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NsiI A_TGCA'T
Cuts at: 2661 2661
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 3371 4916 1132
Size: 97 2142 1545 2229
Fragments arranged by size:
2229 2142 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6013
PciI A'CATG_T
Cuts at: 4912 4912
Size: 6013
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2179 1108
Size: 1071 4942
PfoI T'CCnGG_A
Cuts at: 2352 2352
Size: 6013
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2143 4082 4114 5595 5627 2111
Size: 32 1939 32 1481 32 2497
Fragments arranged by size:
2497 1939 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2589 2589
Size: 6013
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PstI C_TGCA'G
Cuts at: 830 3365 830
Size: 2535 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SacI G_AGCT'C
Cuts at: 2374 2998 2374
Size: 624 5389
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 4261 5973 4261
Size: 1712 4301
SfcI C'TryA_G
Cuts at: 826 1045 2089 3361 4496 5177 5368 826
Size: 219 1044 1272 1135 681 191 1471
Fragments arranged by size:
1471 1272 1135 1044 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 3340 1872
Size: 1468 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4117 5018 5280 820
Size: 197 926 2174 901 262 1553
Fragments arranged by size:
2174 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2004 3452 513
Size: 552 939 1448 3074
Fragments arranged by size:
3074 1448 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 2119 3120 4040 4057 4210 4395
Size: 1255 480 59 1001 920 17 153 185
Cuts at: 4395 4840 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1001 920 480 445 185 153
59 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3107 4259 5971
Size: 274 80 33 51 1443 1130 1152 1712
Cuts at: 5971 96
Size: 138
Fragments arranged by size:
1712 1443 1152 1130 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5973 6005 5973
Size: 32 5981
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 3338 1870
Size: 1468 4545
XmnI GAAnn'nnTTC
Cuts at: 2333 4142 2333
Size: 1809 4204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI