Restriction map for
pFN21AB7736
(Circular) (Six-base) MAPSORT of: pFN21AB7736.seq Check: 7265 from: 1 to: 6151
pFN21AB7736 6151 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2342 3494 1937
Size: 405 1152 4594
Fragments arranged by size:
4594 1152 405
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 2289 2925 4278 4651 1563
Size: 726 636 1353 373 3063
Fragments arranged by size:
3063 1353 726 636 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3294 4214 5598 5866 1632
Size: 1662 920 1384 268 1917
Fragments arranged by size:
1917 1662 1384 920 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 2253 5050 6027 1203
Size: 1050 2797 977 1327
Fragments arranged by size:
2797 1327 1050 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 2541 1166
Size: 1375 4776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2291 1184
Size: 1107 5044
AlwNI CAG_nnn'CTG
Cuts at: 2627 3100 3894 5466 2627
Size: 473 794 1572 3312
Fragments arranged by size:
3312 1572 794 473
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2225 2861 3460 3686 3838 5947 1493
Size: 732 636 599 226 152 2109 1697
Fragments arranged by size:
2109 1697 732 636 599 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 3472 4927 618
Size: 325 217 627 234 1451 1455 1842
Fragments arranged by size:
1842 1455 1451 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 3470 1391
Size: 2079 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3204 928
Size: 971 1305 3875
Fragments arranged by size:
3875 1305 971
BbvCI CC'TCA_GC
Cuts at: 2187 2187
Size: 6151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2959 4043 5253 2959
Size: 1084 1210 3857
Fragments arranged by size:
3857 1210 1084
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 2024 4212 5368 1790
Size: 234 2188 1156 2573
Fragments arranged by size:
2573 2188 1156 234
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2637 3882 4840
Size: 416 53 83 399 24 1220 1245 958
Cuts at: 4840 442
Size: 1753
Fragments arranged by size:
1753 1245 1220 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3101 4811 1239
Size: 1862 1710 2579
Fragments arranged by size:
2579 1862 1710
Bpu10I CC'TnA_GC
Cuts at: 2187 2187
Size: 6151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4276 5141 5439 4276
Size: 865 298 4988
Fragments arranged by size:
4988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2147 4814 882
Size: 617 648 2667 2219
Fragments arranged by size:
2667 2219 648 617
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 6145 6145
Size: 6151
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3165 3958 4340 4975
Size: 53 83 186 936 1632 793 382 635
Cuts at: 4975 275
Size: 1451
Fragments arranged by size:
1632 1451 936 793 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3428 3458 1178
Size: 30 165 30 2025 30 3871
Fragments arranged by size:
3871 2025 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 3191 3220 3293 3380 3404 3419 3449
Size: 1018 987 29 73 87 24 15 30
Cuts at: 3449 1186
Size: 3888
Fragments arranged by size:
3888 1018 987 87 73 30 29 24
15
BseYI C'CCAG_C
Cuts at: 2411 2896 5354 5850 2411
Size: 485 2458 496 2712
Fragments arranged by size:
2712 2458 496 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2405 1163
Size: 573 669 4909
Fragments arranged by size:
4909 669 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4212 4297 5368 4212
Size: 85 1071 4995
Fragments arranged by size:
4995 1071 85
BsmI GAATG_Cn'
Cuts at: 3123 3662 3755 3123
Size: 539 93 5519
Fragments arranged by size:
5519 539 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2685 2685
Size: 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 3470 4212 4297 5368 1391
Size: 399 234 1446 742 85 1071 2174
Fragments arranged by size:
2174 1446 1071 742 399 234 85
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 2583 4040 5770 2583
Size: 1457 1730 2964
Fragments arranged by size:
2964 1730 1457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2889 3506 844
Size: 2045 617 3489
Fragments arranged by size:
3489 2045 617
BsrBI CCG'CTC
Cuts at: 1394 1823 2686 4038 1394
Size: 429 863 1352 3507
Fragments arranged by size:
3507 1352 863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4646 4820 65
Size: 1183 3398 174 1396
Fragments arranged by size:
3398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2731 4795 4982 1700
Size: 110 921 2064 187 2869
Fragments arranged by size:
2869 2064 921 187 110
BsrGI T'GTAC_A
Cuts at: 96 2517 96
Size: 2421 3730
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 2857 2749
Size: 108 6043
BstYI r'GATC_y
Cuts at: 1972 2973 3481 3848 4233 4250 5702 5788 5800
Size: 1001 508 367 385 17 1452 86 12
Cuts at: 5800 6146 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 508 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3159 3174 3159
Size: 15 6136
BtgI C'CryG_G
Cuts at: 513 1065 2004 2539 2571 513
Size: 552 939 535 32 4093
Fragments arranged by size:
4093 939 552 535 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2226 2380 3201 487
Size: 683 56 1000 154 821 3437
Fragments arranged by size:
3437 1000 821 683 154 56
BtsI GCAGTG_nn'
Cuts at: 3667 4461 4481 3667
Size: 794 20 5337
Fragments arranged by size:
5337 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2478 3447 3598
Size: 54 1425 50 275 18 648 969 151
Cuts at: 3598 4487 6116 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 969 889 648 275 151 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 3252 3270 3310 3381 4081 1353
Size: 1197 702 18 40 71 700 3423
Fragments arranged by size:
3423 1197 702 700 71 40 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4738 5112 5258 387
Size: 1064 138 3149 374 146 1280
Fragments arranged by size:
3149 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3101 3294 4214 4811 5598 5866 1239
Size: 393 1469 193 920 597 787 268 1524
Fragments arranged by size:
1524 1469 920 787 597 393 268 193
EcoO109I rG'GnC_Cy
Cuts at: 2416 2632 2416
Size: 216 5935
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
EcoRV GAT'ATC
Cuts at: 2470 2644 2470
Size: 174 5977
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 5298 1678
Size: 3620 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2693 2725 2786
Size: 32 135 32 668 32 421 32 61
Cuts at: 2786 2818 4792 4824 4866 4898 1373
Size: 32 1974 32 42 32 2626
Fragments arranged by size:
2626 1974 668 421 135 61 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3495 3741 669
Size: 988 281 739 818 246 3079
Fragments arranged by size:
3079 988 818 739 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 2677 3741 2677
Size: 1064 5087
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2041 2238 3333 5265 5449 1305
Size: 736 197 1095 1932 184 2007
Fragments arranged by size:
2007 1932 1095 736 197 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2480 10
Size: 54 1425 343 648 3681
Fragments arranged by size:
3681 1425 648 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 2399 2541 2588 2604 4109 4468
Size: 648 1045 188 142 47 16 1505 359
Cuts at: 4468 4627 518
Size: 159 2042
Fragments arranged by size:
2042 1505 1045 648 359 188 159 142
47 16
MspA1I CmG'CkG
Cuts at: 1606 2954 3567 4244 5392 5637 1606
Size: 1348 613 677 1148 245 2120
Fragments arranged by size:
2120 1348 1148 677 613 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2539 513
Size: 552 939 535 4125
Fragments arranged by size:
4125 939 552 535
NdeI CA'TA_TG
Cuts at: 387 2944 387
Size: 2557 3594
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2257 3509 5054 1132
Size: 97 1028 1252 1545 2229
Fragments arranged by size:
2229 1545 1252 1028 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 2253 5050 2253
Size: 2797 3354
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2627 1108
Size: 1519 4632
PfoI T'CCnGG_A
Cuts at: 2484 2484
Size: 6151
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 4220 4252 5733 5765 3245
Size: 32 943 32 1481 32 3631
Fragments arranged by size:
3631 1481 943 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 6151
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PstI C_TGCA'G
Cuts at: 830 3503 830
Size: 2673 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
PvuII CAG'CTG
Cuts at: 2954 2954
Size: 6151
RsrII CG'GwC_CG
Cuts at: 2575 2575
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 2615 4399 6111 2615
Size: 1784 1712 2655
Fragments arranged by size:
2655 1784 1712
SfcI C'TryA_G
Cuts at: 826 1045 3499 4634 5315 5506 826
Size: 219 2454 1135 681 191 1471
Fragments arranged by size:
2454 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4255 5156 5418 820
Size: 197 926 2312 901 262 1553
Fragments arranged by size:
2312 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 2561 1517
Size: 1044 5107
StyI C'CwwG_G
Cuts at: 513 1065 2004 2539 3399 3590 513
Size: 552 939 535 860 191 3074
Fragments arranged by size:
3074 939 860 552 535 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2103 4178 4195 4348 4533 4978 325
Size: 1255 523 2075 17 153 185 445 1498
Fragments arranged by size:
2075 1498 1255 523 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 2517 2613
Size: 274 80 33 51 1443 285 255 96
Cuts at: 2613 4397 6109 96
Size: 1784 1712 138
Fragments arranged by size:
1784 1712 1443 285 274 255 138 96
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 6111 6143 3123
Size: 32 2956 32 3131
Fragments arranged by size:
3131 2956 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2579 2590 1316
Size: 1263 11 4877
Fragments arranged by size:
4877 1263 11
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI