Restriction map for
pFN21AB7727
(Circular) (Six-base) MAPSORT of: pFN21AB7727.seq Check: 3149 from: 1 to: 5998
pFN21AB7727 5998 bp
With 182 enzymes: SgfI *
March 28, 2012 12:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2629 3003 3341 1937
Size: 692 374 338 4594
Fragments arranged by size:
4594 692 374 338
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2903 3213 3222 3250 4061 5445 5713 1632
Size: 1271 310 9 28 811 1384 268 1917
Fragments arranged by size:
1917 1384 1271 811 310 268 28 9
AfeI AGC'GCT
Cuts at: 2260 2260
Size: 5998
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 2210 4829 2210
Size: 2619 3379
AhdI GACnn_n'nnGTC
Cuts at: 3247 4727 3247
Size: 1480 4518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AlwNI CAG_nnn'CTG
Cuts at: 2815 2926 3741 5313 2815
Size: 111 815 1572 3500
Fragments arranged by size:
3500 1572 815 111
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 3307 3533 3685 5794 1493
Size: 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2164 3323 1870
Size: 73 221 1159 4545
Fragments arranged by size:
4545 1159 221 73
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3319 4774 618
Size: 325 217 627 381 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2316 3317 1391
Size: 925 1001 4072
Fragments arranged by size:
4072 1001 925
BbeI G_GCGC'C
Cuts at: 2172 2172
Size: 5998
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2826 2860 4189 4223 1903
Size: 34 889 34 1329 34 3678
Fragments arranged by size:
3678 1329 889 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 4609 136
Size: 107 122 71 631 605 487 2450 1525
Fragments arranged by size:
2450 1525 631 605 487 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2501 4059 5215 1790
Size: 711 1558 1156 2573
Fragments arranged by size:
2573 1558 1156 711
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3729 4687 442
Size: 416 53 83 399 24 2312 958 1753
Fragments arranged by size:
2312 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2837 3007 4658 1239
Size: 1091 507 170 1651 2579
Fragments arranged by size:
2579 1651 1091 507 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2886 4123 4988 5286 2357
Size: 529 1237 865 298 3069
Fragments arranged by size:
3069 1237 865 529 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 3805 4187 4822
Size: 53 83 186 936 636 1636 382 635
Cuts at: 4822 275
Size: 1451
Fragments arranged by size:
1636 1451 936 636 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2210 3102 4431 4829 5103 5250 5661 2210
Size: 892 1329 398 274 147 411 2547
Fragments arranged by size:
2547 1329 892 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2353 2383 1178
Size: 30 165 30 950 30 4793
Fragments arranged by size:
4793 950 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2612 1186
Size: 1426 4572
BseYI C'CCAG_C
Cuts at: 2268 2880 5201 5697 2268
Size: 612 2321 496 2569
Fragments arranged by size:
2569 2321 612 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 3139 1163
Size: 573 606 797 4022
Fragments arranged by size:
4022 797 606 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2236 4059 4144 5215 2236
Size: 1823 85 1071 3019
Fragments arranged by size:
3019 1823 1071 85
BsmI GAATG_Cn'
Cuts at: 2348 3509 3602 2348
Size: 1161 93 4744
Fragments arranged by size:
4744 1161 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2236 2316 2501 3317 4059 4144 5215
Size: 399 446 80 185 816 742 85 1071
Cuts at: 5215 1391
Size: 2174
Fragments arranged by size:
2174 1071 816 742 446 399 185 85
80
BspEI T'CCGG_A
Cuts at: 3102 5661 3102
Size: 2559 3439
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2934 3353 844
Size: 2090 419 3489
Fragments arranged by size:
3489 2090 419
BsrBI CCG'CTC
Cuts at: 1394 1823 2887 3885 1394
Size: 429 1064 998 3507
Fragments arranged by size:
3507 1064 998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2450 2802 4493 4667 65
Size: 1183 1202 352 1691 174 1396
Fragments arranged by size:
1691 1396 1202 1183 352 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2210 4642 4829 1700
Size: 110 297 103 2432 187 2869
Fragments arranged by size:
2869 2432 297 187 110 103
BsrGI T'GTAC_A
Cuts at: 96 2491 96
Size: 2395 3603
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 2667 3216 1184
Size: 1483 549 3966
Fragments arranged by size:
3966 1483 549
BstXI CCAn_nnnn'nTGG
Cuts at: 2468 2797 2468
Size: 329 5669
BstYI r'GATC_y
Cuts at: 1972 2395 3099 3328 3695 4080 4097 5549 5635
Size: 423 704 229 367 385 17 1452 86
Cuts at: 5635 5647 5993 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 704 423 385 367 346 229
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3109 3109
Size: 5998
BtgI C'CryG_G
Cuts at: 513 1065 2018 2202 513
Size: 552 953 184 4309
Fragments arranged by size:
4309 953 552 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2833 487
Size: 683 56 1607 3652
Fragments arranged by size:
3652 1607 683 56
BtsI GCAGTG_nn'
Cuts at: 3514 4308 4328 3514
Size: 794 20 5184
Fragments arranged by size:
5184 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2799 3445 4334
Size: 54 1425 50 275 18 969 646 889
Cuts at: 4334 5963 8
Size: 1629 43
Fragments arranged by size:
1629 1425 969 889 646 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2991 3928 1353
Size: 1638 937 3423
Fragments arranged by size:
3423 1638 937
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2330 2837 2903 3007 3213 3222 3250
Size: 393 698 507 66 104 206 9 28
Cuts at: 3250 4061 4658 5445 5713 1239
Size: 811 597 787 268 1524
Fragments arranged by size:
1524 811 787 698 597 507 393 268
206 104 66 28 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 2585
Size: 5998
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2351 2351
Size: 5998
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 2172 2262 3045 5145 1678
Size: 494 90 783 2100 2531
Fragments arranged by size:
2531 2100 783 494 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3056 3088 3233 3265 4639
Size: 32 135 32 1484 32 145 32 1374
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1484 1374 145 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2749 3342 3588 669
Size: 988 281 811 593 246 3079
Fragments arranged by size:
3079 988 811 593 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KasI G'GCGC_C
Cuts at: 2168 2168
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5112 5296 1305
Size: 3807 184 2007
Fragments arranged by size:
3807 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2801 10
Size: 54 1425 343 969 3207
Fragments arranged by size:
3207 1425 969 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2515 2694 3956 4315 4474 518
Size: 648 1349 179 1262 359 159 2042
Fragments arranged by size:
2042 1349 1262 648 359 179 159
MspA1I CmG'CkG
Cuts at: 1606 2020 3414 4091 5239 5484 1606
Size: 414 1394 677 1148 245 2120
Fragments arranged by size:
2120 1394 1148 677 414 245
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5998
NarI GG'CG_CC
Cuts at: 2169 2169
Size: 5998
NcoI C'CATG_G
Cuts at: 513 1065 2202 513
Size: 552 1137 4309
Fragments arranged by size:
4309 1137 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 3158 3356 4901 1132
Size: 97 1929 198 1545 2229
Fragments arranged by size:
2229 1929 1545 198 97
PasI CC'CwG_GG
Cuts at: 1348 2081 1348
Size: 733 5265
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2590 2664 1108
Size: 1482 74 4442
Fragments arranged by size:
4442 1482 74
PfoI T'CCnGG_A
Cuts at: 2541 2541
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2167 2199 4067 4099 5580 5612 2167
Size: 32 1868 32 1481 32 2553
Fragments arranged by size:
2553 1868 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 5966
PstI C_TGCA'G
Cuts at: 830 2046 3350 830
Size: 1216 1304 3478
Fragments arranged by size:
3478 1304 1216
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
SacII CC_GC'GG
Cuts at: 2021 2021
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SexAI A'CCwGG_T
Cuts at: 2793 2793
Size: 5998
SfcI C'TryA_G
Cuts at: 826 1045 2042 2227 3346 4481 5162 5353 826
Size: 219 997 185 1119 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 997 681 219 191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 2159 2159
Size: 5998
SfoI GGC'GCC
Cuts at: 2170 2170
Size: 5998
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 2901 4102 5003 5265 820
Size: 197 926 393 565 1201 901 262 1553
Fragments arranged by size:
1553 1201 926 901 565 393 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 2761 3922 5
Size: 47 2709 1161 2081
Fragments arranged by size:
2709 2081 1161 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2202 2941 3437 513
Size: 552 1137 739 496 3074
Fragments arranged by size:
3074 1137 739 552 496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2289 2439 2954 4025 4042 4195 4380
Size: 1255 709 150 515 1071 17 153 185
Cuts at: 4380 4825 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1071 709 515 445 185 153
150 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2491 4244 5956
Size: 274 80 33 51 1443 514 1753 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
1753 1712 1443 514 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2353 2385 3002 3034 5958 5990 2353
Size: 32 617 32 2924 32 2361
Fragments arranged by size:
2924 2361 617 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 2677 3334 2677
Size: 657 5341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3090 3090
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PsrI PstI PvuI SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PvuII RsrII SacI SanDI SapI SrfI
SwaI