Restriction map for
pFN21AB7726
(Circular) (Six-base) MAPSORT of: pFN21AB7726.seq Check: 167 from: 1 to: 6145
pFN21AB7726 6145 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3285 3285
Size: 6145
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2124 3955 278
Size: 53 83 186 936 588 1831 2468
Fragments arranged by size:
2468 1831 936 588 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4208 5592 5860 1632
Size: 2576 1384 268 1917
Fragments arranged by size:
2576 1917 1384 268
AfeI AGC'GCT
Cuts at: 2034 2034
Size: 6145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 3416 1166
Size: 2250 3895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2444 1184
Size: 1260 4885
AlwNI CAG_nnn'CTG
Cuts at: 2324 3888 5460 2324
Size: 1564 1572 3009
Fragments arranged by size:
3009 1572 1564
ApaI G_GGCC'C
Cuts at: 3444 3444
Size: 6145
ApaLI G'TGCA_C
Cuts at: 3053 3419 4202 5358 3053
Size: 366 783 1156 3840
Fragments arranged by size:
3840 1156 783 366
ApoI r'AATT_y
Cuts at: 1493 2903 3454 3680 3832 5941 1493
Size: 1410 551 226 152 2109 1697
Fragments arranged by size:
2109 1697 1410 551 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2246 3434 3470 1870
Size: 73 303 1188 36 4545
Fragments arranged by size:
4545 1188 303 73 36
BamHI G'GATC_C
Cuts at: 2109 3475 3842 2109
Size: 1366 367 4412
Fragments arranged by size:
4412 1366 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3466 4921 618
Size: 325 217 627 1679 1455 1842
Fragments arranged by size:
1842 1679 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2815 2884 3097 3444 3464 1391
Size: 1424 69 213 347 20 4072
Fragments arranged by size:
4072 1424 347 213 69 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2985 3003 928
Size: 971 1086 18 4070
Fragments arranged by size:
4070 1086 971 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 136
Size: 107 122 71 631 605 3084 1525
Fragments arranged by size:
3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 3057 3423 3444 4206 5362 1790
Size: 1267 366 21 762 1156 2573
Fragments arranged by size:
2573 1267 1156 762 366 21
BmgBI CAC'GTC
Cuts at: 1318 2074 2233 3171 1318
Size: 756 159 938 4292
Fragments arranged by size:
4292 938 756 159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2544 2682 3876
Size: 416 53 83 399 24 1127 138 1194
Cuts at: 3876 4834 442
Size: 958 1753
Fragments arranged by size:
1753 1194 1127 958 416 399 138 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 2689 2799 4805 1239
Size: 1062 388 110 2006 2579
Fragments arranged by size:
2579 2006 1062 388 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2835 3272 4270 5135 5433 2835
Size: 437 998 865 298 3547
Fragments arranged by size:
3547 998 865 437 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 3214 3936 493
Size: 711 1167 843 722 2702
Fragments arranged by size:
2702 1167 843 722 711
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 3952 4334 4969
Size: 53 83 186 936 588 1831 382 635
Cuts at: 4969 275
Size: 1451
Fragments arranged by size:
1831 1451 936 635 588 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3311 4578 4976 5250 5397 5808 3311
Size: 1267 398 274 147 411 3648
Fragments arranged by size:
3648 1267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2211 2241 2455 2485 2687
Size: 30 165 30 808 30 214 30 202
Cuts at: 2687 2717 1178
Size: 30 4606
Fragments arranged by size:
4606 808 214 202 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2056 2825 1186
Size: 870 769 4506
Fragments arranged by size:
4506 870 769
BseYI C'CCAG_C
Cuts at: 2035 2077 5348 5844 2035
Size: 42 3271 496 2336
Fragments arranged by size:
3271 2336 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2310 2816 1163
Size: 573 574 506 4492
Fragments arranged by size:
4492 574 573 506
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2346 3595 4356 5384 1540
Size: 275 186 345 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 345 275 186
BsiHKAI G_wGCw'C
Cuts at: 2158 2238 2370 2945 3057 3423 4206 4291 5362
Size: 80 132 575 112 366 783 85 1071
Cuts at: 5362 2158
Size: 2941
Fragments arranged by size:
2941 1071 783 575 366 132 112 85
80
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
BsmBI CGTCTCn'nnnn_
Cuts at: 2688 3161 2688
Size: 473 5672
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2238 2370 2815 2884 2945 3057
Size: 399 368 80 132 445 69 61 112
Cuts at: 3057 3097 3423 3444 3464 4206 4291 5362 1391
Size: 40 326 21 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 445 399 368 326 132
112 85 80 69 61 40 21 20
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3263 3285 3500 844
Size: 2419 22 215 3489
Fragments arranged by size:
3489 2419 215 22
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4640 4814 65
Size: 1183 3392 174 1396
Fragments arranged by size:
3392 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3006 3105 4789 4976 1700
Size: 110 1196 99 1684 187 2869
Fragments arranged by size:
2869 1684 1196 187 110 99
BsrGI T'GTAC_A
Cuts at: 96 2292 3187 96
Size: 2196 895 3054
Fragments arranged by size:
3054 2196 895
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6145
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2109 3475 3842 4227 4244 5696 5782 5794
Size: 137 1366 367 385 17 1452 86 12
Cuts at: 5794 6140 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1366 385 367 346 137 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4654
Fragments arranged by size:
4654 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2158 487
Size: 683 56 932 4474
Fragments arranged by size:
4474 932 683 56
BtsI GCAGTG_nn'
Cuts at: 2188 2322 2743 3076 3328 3661 4455 4475 2188
Size: 134 421 333 252 333 794 20 3858
Fragments arranged by size:
3858 794 421 333 333 252 134 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 3107 3592
Size: 54 1425 50 275 18 513 764 485
Cuts at: 3592 4481 6110 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 764 513 485 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2343 3592 1537
Size: 275 531 1249 4090
Fragments arranged by size:
4090 1249 531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4075 1353
Size: 2722 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3260 4732 5106 5252 387
Size: 1064 138 1671 1472 374 146 1280
Fragments arranged by size:
1671 1472 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2689 2799 4208 4805 5592 5860
Size: 393 669 388 110 1409 597 787 268
Cuts at: 5860 1239
Size: 1524
Fragments arranged by size:
1524 1409 787 669 597 393 388 268
110
EcoNI CCTnn'n_nnAGG
Cuts at: 2475 2475
Size: 6145
EcoO109I rG'GnC_Cy
Cuts at: 2252 2543 2747 3440 2252
Size: 291 204 693 4957
Fragments arranged by size:
4957 693 291 204
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2036 2494 5292 1678
Size: 358 458 2798 2531
Fragments arranged by size:
2798 2531 458 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2222 2254 2687 2719 4786
Size: 32 135 32 650 32 433 32 2067
Cuts at: 4786 4818 4860 4892 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2067 650 433 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2663 3368 3489 3735 669
Size: 988 281 725 705 121 246 3079
Fragments arranged by size:
3079 988 725 705 281 246 121
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 2804 5259 5443 1305
Size: 789 710 2455 184 2007
Fragments arranged by size:
2455 2007 789 710 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4323
Fragments arranged by size:
4323 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3416 4103 4462 4621 518
Size: 648 2250 687 359 159 2042
Fragments arranged by size:
2250 2042 687 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2913 3001 3561 4238 5386 5631 1606
Size: 1307 88 560 677 1148 245 2120
Fragments arranged by size:
2120 1307 1148 677 560 245 88
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4654
Fragments arranged by size:
4654 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 2343 3592 2343
Size: 1249 4896
NspI r_CATG'y
Cuts at: 1132 1229 2385 3503 5048 1132
Size: 97 1156 1118 1545 2229
Fragments arranged by size:
2229 1545 1156 1118 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6145
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PmlI CAC'GTG
Cuts at: 2371 3214 2371
Size: 843 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2252 2747 2252
Size: 495 5650
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PspOMI G'GGCC_C
Cuts at: 3440 3440
Size: 6145
PstI C_TGCA'G
Cuts at: 830 2183 2537 3282 3497 830
Size: 1353 354 745 215 3478
Fragments arranged by size:
3478 1353 745 354 215
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2913 3001 2913
Size: 88 6057
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SanDI GG'GwC_CC
Cuts at: 2252 2252
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SexAI A'CCwGG_T
Cuts at: 2476 2476
Size: 6145
SfcI C'TryA_G
Cuts at: 826 1045 2179 2533 3278 3493 4628 5309 5500
Size: 219 1134 354 745 215 1135 681 191
Cuts at: 5500 826
Size: 1471
Fragments arranged by size:
1471 1135 1134 745 681 354 219 215
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 2248 3472 1872
Size: 376 1224 4545
Fragments arranged by size:
4545 1224 376
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2850 3287 4249 5150 5412 820
Size: 197 926 907 437 962 901 262 1553
Fragments arranged by size:
1553 962 926 907 901 437 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 2385 3503 1132
Size: 1253 1118 3774
Fragments arranged by size:
3774 1253 1118
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 2576 2621 2702 1517
Size: 1059 45 81 4960
Fragments arranged by size:
4960 1059 81 45
StyI C'CwwG_G
Cuts at: 513 1065 2004 2571 2961 3584 513
Size: 552 939 567 390 623 3074
Fragments arranged by size:
3074 939 623 567 552 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2520 4172 4189 4342 4527 4972 325
Size: 1255 940 1652 17 153 185 445 1498
Fragments arranged by size:
1652 1498 1255 940 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 2592 2598
Size: 274 80 33 51 1443 315 300 6
Cuts at: 2598 3187 4391 6103 96
Size: 589 1204 1712 138
Fragments arranged by size:
1712 1443 1204 589 315 300 274 138
80 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2211 2243 2319 2351 6105 6137 2211
Size: 32 76 32 3754 32 2219
Fragments arranged by size:
3754 2219 76 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 2246 3470 1870
Size: 376 1224 4545
Fragments arranged by size:
4545 1224 376
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2122 3953 276
Size: 53 83 186 936 588 1831 2468
Fragments arranged by size:
2468 1831 936 588 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspXI PsrI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI XcmI