Restriction map for
pFN21AB7723
(Circular) (Six-base) MAPSORT of: pFN21AB7723.seq Check: 7751 from: 1 to: 5995
pFN21AB7723 5995 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2702 2702
Size: 5995
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 3294 4122 4495 1563
Size: 1731 828 373 3063
Fragments arranged by size:
3063 1731 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2702 2740 3225 4058 5442 5710 1632
Size: 1070 38 485 833 1384 268 1917
Fragments arranged by size:
1917 1384 1070 833 485 268 38
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 3066 4894 5871 1203
Size: 1863 1828 977 1327
Fragments arranged by size:
1863 1828 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5995
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 2206 2423 2432 2441 3032 3059 3738 5310 2206
Size: 217 9 9 591 27 679 1572 2891
Fragments arranged by size:
2891 1572 679 591 217 27 9 9
ApaI G_GGCC'C
Cuts at: 2087 2394 2087
Size: 307 5688
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 3304 3530 3682 5791 1493
Size: 1811 226 152 2109 1697
Fragments arranged by size:
2109 1811 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2393 3167 3320 1870
Size: 73 194 256 774 153 4545
Fragments arranged by size:
4545 774 256 194 153 73
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3316 4771 618
Size: 325 217 627 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2318 2394 3090 3314 1391
Size: 696 231 76 696 224 4072
Fragments arranged by size:
4072 696 696 231 224 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BbvCI CC'TCA_GC
Cuts at: 2303 2585 2303
Size: 282 5713
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 4606 136
Size: 107 122 71 631 605 380 2554 1525
Fragments arranged by size:
2554 1525 631 605 380 122 107 71
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 2410 3412 2410
Size: 1002 4993
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2394 4056 5212 1790
Size: 297 307 1662 1156 2573
Fragments arranged by size:
2573 1662 1156 307 297
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2030 3726 4684
Size: 416 53 83 399 24 613 1696 958
Cuts at: 4684 442
Size: 1753
Fragments arranged by size:
1753 1696 958 613 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2019 2552 3081 4655 1239
Size: 780 533 529 1574 2579
Fragments arranged by size:
2579 1574 780 533 529
Bpu10I CC'TnA_GC
Cuts at: 2303 2585 2303
Size: 282 5713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4120 4985 5283 4120
Size: 865 298 4832
Fragments arranged by size:
4832 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2103 2881 4658 882
Size: 617 604 778 1777 2219
Fragments arranged by size:
2219 1777 778 617 604
BsaAI yAC'GTr
Cuts at: 493 1204 3786 493
Size: 711 2582 2702
Fragments arranged by size:
2702 2582 711
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3802 4184 4819 275
Size: 53 83 186 936 2269 382 635 1451
Fragments arranged by size:
2269 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4428 4826 5100 5247 5658 4428
Size: 398 274 147 411 4765
Fragments arranged by size:
4765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5770
Fragments arranged by size:
5770 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2342 2840 2852 2951 1186
Size: 1153 3 498 12 99 4230
Fragments arranged by size:
4230 1153 498 99 12 3
BseYI C'CCAG_C
Cuts at: 2387 5198 5694 2387
Size: 2811 496 2688
Fragments arranged by size:
2811 2688 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5422
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3445 4206 5234 1540
Size: 275 186 1444 761 1028 2301
Fragments arranged by size:
2301 1444 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2318 2693 4056 4141 5212 2318
Size: 375 1363 85 1071 3101
Fragments arranged by size:
3101 1363 1071 375 85
BsmI GAATG_Cn'
Cuts at: 2307 3506 3599 2307
Size: 1199 93 4703
Fragments arranged by size:
4703 1199 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2318 2394 2693 3090 3314 4056
Size: 399 297 231 76 299 397 224 742
Cuts at: 4056 4141 5212 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 299 297 231
224 85 76
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 2574 3884 5614 2574
Size: 1310 1730 2955
Fragments arranged by size:
2955 1730 1310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2589 2702 3350 844
Size: 1745 113 648 3489
Fragments arranged by size:
3489 1745 648 113
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 1394
Size: 429 2059 3507
Fragments arranged by size:
3507 2059 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4490 4664 65
Size: 1183 3242 174 1396
Fragments arranged by size:
3242 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2332 4639 4826 1700
Size: 110 522 2307 187 2869
Fragments arranged by size:
2869 2307 522 187 110
BsrGI T'GTAC_A
Cuts at: 96 3183 96
Size: 3087 2908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2423 2432 794
Size: 1629 9 4357
Fragments arranged by size:
4357 1629 9
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5995
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 2626 2611
Size: 15 5980
BstYI r'GATC_y
Cuts at: 1972 2505 3056 3325 3692 4077 4094 5546 5632
Size: 533 551 269 367 385 17 1452 86
Cuts at: 5632 5644 5990 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 551 533 385 367 346 269
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2610 3191 513
Size: 552 939 606 581 3317
Fragments arranged by size:
3317 939 606 581 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2836 2871 529
Size: 35 2272 35 3653
Fragments arranged by size:
3653 2272 35 35
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2968 5002 809
Size: 507 1652 2034 1802
Fragments arranged by size:
2034 1802 1652 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3442 4331 5960
Size: 54 1425 50 275 18 1612 889 1629
Cuts at: 5960 8
Size: 43
Fragments arranged by size:
1629 1612 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3442 1537
Size: 275 1630 4090
Fragments arranged by size:
4090 1630 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3244 3925 1353
Size: 1891 681 3423
Fragments arranged by size:
3423 1891 681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2121 4582 4956 5102 387
Size: 1064 138 532 2461 374 146 1280
Fragments arranged by size:
2461 1280 1064 532 374 146 138
EcoICRI GAG'CTC
Cuts at: 2316 2316
Size: 5995
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 2552 2702 2740 3081 3225 4058
Size: 393 387 533 150 38 341 144 833
Cuts at: 4058 4655 5442 5710 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 833 787 597 533 393 387 341
268 150 144 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2210
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 2053 2083 2084 2134 2776 2053
Size: 30 1 50 642 5272
Fragments arranged by size:
5272 642 50 30 1
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 2667 2749 5142 1678
Size: 989 82 2393 2531
Fragments arranged by size:
2531 2393 989 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373
Size: 32 135 32 3064 32 42 32 2626
Fragments arranged by size:
3064 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2904 3154 3315 5109 5293 1305
Size: 1599 250 161 1794 184 2007
Fragments arranged by size:
2007 1794 1599 250 184 161
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2672 2864 3953 4312 4471 518
Size: 648 1506 192 1089 359 159 2042
Fragments arranged by size:
2042 1506 1089 648 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2347 2423 2432 2680 3216 3411 4088 5236
Size: 741 76 9 248 536 195 677 1148
Cuts at: 5236 5481 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 741 677 536 248 245 195
76 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 2368 2553 3070 3353 4898 1132
Size: 97 1139 185 517 283 1545 2229
Fragments arranged by size:
2229 1545 1139 517 283 185 97
PasI CC'CwG_GG
Cuts at: 1348 2980 1348
Size: 1632 4363
PciI A'CATG_T
Cuts at: 3066 4894 3066
Size: 1828 4167
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 1108
Size: 1098 4897
PfoI T'CCnGG_A
Cuts at: 2727 2727
Size: 5995
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2776 2134
Size: 642 5353
PshAI GACnn'nnGTC
Cuts at: 1613 2570 1613
Size: 957 5038
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 2083 2390 2083
Size: 307 5688
PstI C_TGCA'G
Cuts at: 830 2178 2428 2437 3028 3347 830
Size: 1348 250 9 591 319 3478
Fragments arranged by size:
3478 1348 591 319 250 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2347 2423 2680 3216 2347
Size: 76 257 536 5126
Fragments arranged by size:
5126 536 257 76
SacI G_AGCT'C
Cuts at: 2318 2318
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SanDI GG'GwC_CC
Cuts at: 2134 2134
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2174 2424 2433 3024 3343 4478 5159
Size: 219 1129 250 9 591 319 1135 681
Cuts at: 5159 5350 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1129 681 591 319 250 219
191 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2052 2052
Size: 5995
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 2139 3322 1872
Size: 267 1183 4545
Fragments arranged by size:
4545 1183 267
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4099 5000 5262 820
Size: 197 926 2156 901 262 1553
Fragments arranged by size:
2156 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5995
StyI C'CwwG_G
Cuts at: 513 1065 2004 2832 3434 513
Size: 552 939 828 602 3074
Fragments arranged by size:
3074 939 828 602 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2993 4022 4039 4192 4377 4822 325
Size: 1255 1413 1029 17 153 185 445 1498
Fragments arranged by size:
1498 1413 1255 1029 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3183 4241 5953
Size: 274 80 33 51 1443 1206 1058 1712
Cuts at: 5953 96
Size: 138
Fragments arranged by size:
1712 1443 1206 1058 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 2137 3320 1870
Size: 267 1183 4545
Fragments arranged by size:
4545 1183 267
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SapI SexAI SfoI
SrfI SwaI XcmI