Restriction map for
pFN21AB7719
(Circular) (Six-base) MAPSORT of: pFN21AB7719.seq Check: 4632 from: 1 to: 5986
pFN21AB7719 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 3144 2189
Size: 955 5031
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2131 2348 2568 2813 2984 4049 5433 5701
Size: 499 217 220 245 171 1065 1384 268
Cuts at: 5701 1632
Size: 1917
Fragments arranged by size:
1917 1384 1065 499 268 245 220 217
171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 2853 4885 5862 1203
Size: 1650 2032 977 1327
Fragments arranged by size:
2032 1650 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 2854 3079 4715 2854
Size: 225 1636 4125
Fragments arranged by size:
4125 1636 225
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2048 3152 1184
Size: 864 1104 4018
Fragments arranged by size:
4018 1104 864
AlwNI CAG_nnn'CTG
Cuts at: 3729 5301 3729
Size: 1572 4414
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 3295 3521 3673 5782 1493
Size: 1802 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3124 3311 1870
Size: 73 1181 187 4545
Fragments arranged by size:
4545 1181 187 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3307 4762 618
Size: 325 217 627 1520 1455 1842
Fragments arranged by size:
1842 1520 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2940 3305 1391
Size: 1549 365 4072
Fragments arranged by size:
4072 1549 365
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2211 2245 4177 4211 1903
Size: 34 274 34 1932 34 3678
Fragments arranged by size:
3678 1932 274 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4597 136
Size: 107 122 71 631 605 337 2588 1525
Fragments arranged by size:
2588 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3279 5981 3279
Size: 2702 3284
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 2067 4047 5203 1790
Size: 277 1980 1156 2573
Fragments arranged by size:
2573 1980 1156 277
BmgBI CAC'GTC
Cuts at: 1318 2902 1318
Size: 1584 4402
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3167 1056
Size: 2111 3875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2454 2503 2945 4646 1239
Size: 825 390 49 442 1701 2579
Fragments arranged by size:
2579 1701 825 442 390 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4111 4976 5274 4111
Size: 865 298 4823
Fragments arranged by size:
4823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 2754 4649 882
Size: 617 583 672 1895 2219
Fragments arranged by size:
2219 1895 672 617 583
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2224 2415 3048 3793
Size: 53 83 186 936 691 191 633 745
Cuts at: 3793 4175 4810 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 745 691 635 633 382 191
186 83 53
BsaWI w'CCGG_w
Cuts at: 2055 4419 4817 5091 5238 5649 2055
Size: 2364 398 274 147 411 2392
Fragments arranged by size:
2392 2364 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2537 2567 1178
Size: 30 165 30 1134 30 4597
Fragments arranged by size:
4597 1134 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2354 2357 2396 2539 2542 2545 2999 3002
Size: 1168 3 39 143 3 3 454 3
Cuts at: 3002 3203 1186
Size: 201 3969
Fragments arranged by size:
3969 1168 454 201 143 39 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2531 2670 5189 5685 2531
Size: 139 2519 496 2832
Fragments arranged by size:
2832 2519 496 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2324 3062 3253 1163
Size: 573 588 738 191 3896
Fragments arranged by size:
3896 738 588 573 191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3195 3261 4047 4132 5203 3195
Size: 66 786 85 1071 3978
Fragments arranged by size:
3978 1071 786 85 66
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
BsmBI CGTCTCn'nnnn_
Cuts at: 3025 3025
Size: 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2940 3195 3261 3305 4047 4132
Size: 399 277 873 255 66 44 742 85
Cuts at: 4132 5203 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 873 742 399 277 255 85
66 44
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 3144 3180 3341 844
Size: 1345 955 36 161 3489
Fragments arranged by size:
3489 1345 955 161 36
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2019 2397 3280 4481 4655 65
Size: 1183 771 378 883 1201 174 1396
Fragments arranged by size:
1396 1201 1183 883 771 378 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2241 4630 4817 1700
Size: 110 431 2389 187 2869
Fragments arranged by size:
2869 2389 431 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2998 794
Size: 2204 3782
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 2410
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2070 2620 3279 3316 3683 4068 4085 5537
Size: 98 550 659 37 367 385 17 1452
Cuts at: 5537 5623 5635 5981 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 659 550 385 367 346 98
86 37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2844 513
Size: 552 939 840 3655
Fragments arranged by size:
3655 939 840 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 3089 3502 4296 4316 2227
Size: 862 413 794 20 3897
Fragments arranged by size:
3897 862 794 413 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 3006 3433
Size: 54 1425 50 275 18 189 987 427
Cuts at: 3433 4322 5951 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 987 889 427 275 189 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2587 3916 1353
Size: 1234 1329 3423
Fragments arranged by size:
3423 1329 1234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2131 2348 2454 2503 2568 2813
Size: 393 432 67 217 106 49 65 245
Cuts at: 2813 2945 2984 4049 4646 5433 5701 1239
Size: 132 39 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 787 597 432 393 268 245
217 132 106 67 65 49 39
EcoNI CCTnn'n_nnAGG
Cuts at: 3185 3185
Size: 5986
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 5986
FspI TGC'GCA
Cuts at: 3176 4492 3176
Size: 1316 4670
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 2753 2785 2840
Size: 32 135 32 965 32 184 32 55
Cuts at: 2840 2872 3065 3097 4627 4659 4701 4733 1373
Size: 32 193 32 1530 32 42 32 2626
Fragments arranged by size:
2626 1530 965 193 184 135 55 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2472 3108 5100 5284 1305
Size: 1167 636 1992 184 2007
Fragments arranged by size:
2007 1992 1167 636 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3008 10
Size: 54 1425 343 1176 2988
Fragments arranged by size:
2988 1425 1176 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 2267 3944 4303 4462 518
Size: 648 946 155 1677 359 159 2042
Fragments arranged by size:
2042 1677 946 648 359 159 155
MspA1I CmG'CkG
Cuts at: 1606 3108 3210 3402 4079 5227 5472 1606
Size: 1502 102 192 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 245 192 102
NaeI GCC'GGC
Cuts at: 2243 2243
Size: 5986
NcoI C'CATG_G
Cuts at: 513 1065 2004 2844 513
Size: 552 939 840 3655
Fragments arranged by size:
3655 939 840 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NgoMIV G'CCGG_C
Cuts at: 2241 2241
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 3163 1052
Size: 2111 3875
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2857 2996 3344 4889 1132
Size: 97 1628 139 348 1545 2229
Fragments arranged by size:
2229 1628 1545 348 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 2853 4885 2853
Size: 2032 3954
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PfoI T'CCnGG_A
Cuts at: 2858 3069 2858
Size: 211 5775
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 3096 3338 830
Size: 2266 242 3478
Fragments arranged by size:
3478 2266 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SexAI A'CCwGG_T
Cuts at: 2700 2700
Size: 5986
SfcI C'TryA_G
Cuts at: 826 1045 3092 3334 4469 5150 5341 826
Size: 219 2047 242 1135 681 191 1471
Fragments arranged by size:
2047 1471 1135 681 242 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3126 3313 1872
Size: 1254 187 4545
Fragments arranged by size:
4545 1254 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 4991 5253 820
Size: 197 926 2147 901 262 1553
Fragments arranged by size:
2147 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 2237 2348 1517
Size: 720 111 5155
Fragments arranged by size:
5155 720 111
StyI C'CwwG_G
Cuts at: 513 1065 2004 2211 2844 3425 513
Size: 552 939 207 633 581 3074
Fragments arranged by size:
3074 939 633 581 552 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 4013 4030 4183 4368 4813 325
Size: 1255 526 1907 17 153 185 445 1498
Fragments arranged by size:
1907 1498 1255 526 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 2702 1316
Size: 1386 4600
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2726 2011
Size: 715 5271
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3124 3311 1870
Size: 1254 187 4545
Fragments arranged by size:
4545 1254 187
XmnI GAAnn'nnTTC
Cuts at: 2279 4115 2279
Size: 1836 4150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI