Restriction map for
pFN21AB7715
(Circular) (Six-base) MAPSORT of: pFN21AB7715.seq Check: 1997 from: 1 to: 5983
pFN21AB7715 5983 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2678 2788 2678
Size: 110 5873
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4046 5430 5698 1632
Size: 2414 1384 268 1917
Fragments arranged by size:
2414 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 2295 4814 2295
Size: 2519 3464
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 2558 1166
Size: 1392 4591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2576 3059 1184
Size: 1392 483 4108
Fragments arranged by size:
4108 1392 483
AlwNI CAG_nnn'CTG
Cuts at: 2044 3726 5298 2044
Size: 1682 1572 2729
Fragments arranged by size:
2729 1682 1572
ApaI G_GGCC'C
Cuts at: 2736 2736
Size: 5983
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 2735 3185 3308 1870
Size: 73 732 60 450 123 4545
Fragments arranged by size:
4545 732 450 123 73 60
BamHI G'GATC_C
Cuts at: 2115 3313 3680 2115
Size: 1198 367 4418
Fragments arranged by size:
4418 1198 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2717 2820 3304 4759 618
Size: 325 217 627 930 103 484 1455 1842
Fragments arranged by size:
1842 1455 930 627 484 325 217 103
BanII G_rGCy'C
Cuts at: 1391 2273 2492 2736 3302 1391
Size: 882 219 244 566 4072
Fragments arranged by size:
4072 882 566 244 219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2959 3202 928
Size: 971 347 713 243 3709
Fragments arranged by size:
3709 971 713 347 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2082 3875 5085 2082
Size: 1793 1210 2980
Fragments arranged by size:
2980 1793 1210
BclI T'GATC_A
Cuts at: 1548 2442 2478 2622 1548
Size: 894 36 144 4909
Fragments arranged by size:
4909 894 144 36
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2679 2859 4594
Size: 107 122 71 631 605 1007 180 1735
Cuts at: 4594 136
Size: 1525
Fragments arranged by size:
1735 1525 1007 631 605 180 122 107
71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2736 2823 4044 5200 1790
Size: 946 87 1221 1156 2573
Fragments arranged by size:
2573 1221 1156 946 87
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2765 2846 2963 4643 1239
Size: 1526 81 117 1680 2579
Fragments arranged by size:
2579 1680 1526 117 81
Bpu10I CC'TnA_GC
Cuts at: 2696 2696
Size: 5983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 2148 2621 5977 2148
Size: 473 3356 2154
Fragments arranged by size:
3356 2154 473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2772 3790 4172 4807
Size: 53 83 186 936 1239 1018 382 635
Cuts at: 4807 275
Size: 1451
Fragments arranged by size:
1451 1239 1018 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2013 2112 2295 3067 3213 4416 4814 5088 5235
Size: 99 183 772 146 1203 398 274 147
Cuts at: 5235 5646 2013
Size: 411 2350
Fragments arranged by size:
2350 1203 772 411 398 274 183 147
146 99
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5758
Fragments arranged by size:
5758 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 3203 1186
Size: 925 1092 3966
Fragments arranged by size:
3966 1092 925
BseYI C'CCAG_C
Cuts at: 2549 2780 3146 5186 5682 2549
Size: 231 366 2040 496 2850
Fragments arranged by size:
2850 2040 496 366 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2828 3125 1163
Size: 573 1092 297 4021
Fragments arranged by size:
4021 1092 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2422 2769 3433 4194 5222 1540
Size: 275 186 421 347 664 761 1028 2301
Fragments arranged by size:
2301 1028 761 664 421 347 275 186
BsiHKAI G_wGCw'C
Cuts at: 4044 4129 5200 4044
Size: 85 1071 4827
Fragments arranged by size:
4827 1071 85
BsmI GAATG_Cn'
Cuts at: 2606 3494 3587 2606
Size: 888 93 5002
Fragments arranged by size:
5002 888 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 2492 2736 2823 3302 4044 4129
Size: 399 483 219 244 87 479 742 85
Cuts at: 4129 5200 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 483 479 399 244 219
87 85
BspEI T'CCGG_A
Cuts at: 2112 3067 3213 5646 2112
Size: 955 146 2433 2449
Fragments arranged by size:
2449 2433 955 146
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2678 2788 3338 844
Size: 1834 110 550 3489
Fragments arranged by size:
3489 1834 550 110
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2295 2764 2853 3021 3164 4627 4814
Size: 110 485 469 89 168 143 1463 187
Cuts at: 4814 1700
Size: 2869
Fragments arranged by size:
2869 1463 485 469 187 168 143 110
89
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 3101 4043 5055 3101
Size: 942 1012 4029
Fragments arranged by size:
4029 1012 942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2235 2535 2941 2235
Size: 300 406 5277
Fragments arranged by size:
5277 406 300
BstYI r'GATC_y
Cuts at: 1972 2115 3313 3680 4065 4082 5534 5620 5632
Size: 143 1198 367 385 17 1452 86 12
Cuts at: 5632 5978 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1198 385 367 346 143 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2346 2346
Size: 5983
BtgI C'CryG_G
Cuts at: 513 1065 2913 513
Size: 552 1848 3583
Fragments arranged by size:
3583 1848 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 3499 4293 4313 3499
Size: 794 20 5169
Fragments arranged by size:
5169 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 2194 2206 3287 2194
Size: 12 1081 4890
Fragments arranged by size:
4890 1081 12
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2296 4990 809
Size: 507 980 2694 1802
Fragments arranged by size:
2694 1802 980 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2766 3430 4319
Size: 54 1425 50 275 18 936 664 889
Cuts at: 4319 5948 8
Size: 1629 43
Fragments arranged by size:
1629 1425 936 889 664 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2766 3430 1537
Size: 275 954 664 4090
Fragments arranged by size:
4090 954 664 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 2346 3054 3135 3146 3913 1353
Size: 743 250 708 81 11 767 3423
Fragments arranged by size:
3423 767 743 708 250 81 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2765 2846 2963 4046 4643 5430 5698
Size: 393 1133 81 117 1083 597 787 268
Cuts at: 5698 1239
Size: 1524
Fragments arranged by size:
1524 1133 1083 787 597 393 268 117
81
EcoO109I rG'GnC_Cy
Cuts at: 2740 3181 2740
Size: 441 5542
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2508 2508
Size: 5983
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 5130 1678
Size: 3452 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4624 4656 4698 4730 1373
Size: 32 135 32 3052 32 42 32 2626
Fragments arranged by size:
3052 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2492 5097 5281 1305
Size: 1187 2605 184 2007
Fragments arranged by size:
2605 2007 1187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 2558 3101 3941 4300 4459 518
Size: 648 1308 84 543 840 359 159 2042
Fragments arranged by size:
2042 1308 840 648 543 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2566 2904 3118 3399 4076 5224 5469 1606
Size: 960 338 214 281 677 1148 245 2120
Fragments arranged by size:
2120 1148 960 677 338 281 245 214
NaeI GCC'GGC
Cuts at: 2766 2855 3023 3166 2766
Size: 89 168 143 5583
Fragments arranged by size:
5583 168 143 89
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5431
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NgoMIV G'CCGG_C
Cuts at: 2764 2853 3021 3164 2764
Size: 89 168 143 5583
Fragments arranged by size:
5583 168 143 89
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2071 2110 3341 4886 1132
Size: 97 842 39 1231 1545 2229
Fragments arranged by size:
2229 1545 1231 842 97 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PfoI T'CCnGG_A
Cuts at: 2074 2074
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 2148 3553 2148
Size: 1405 4578
PspOMI G'GGCC_C
Cuts at: 2732 2732
Size: 5983
PstI C_TGCA'G
Cuts at: 830 2903 3000 3335 830
Size: 2073 97 335 3478
Fragments arranged by size:
3478 2073 335 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
PvuII CAG'CTG
Cuts at: 3118 3118
Size: 5983
RsrII CG'GwC_CG
Cuts at: 2293 2293
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SapI GCTCTTCn'nnn_
Cuts at: 2096 2346 3146 2096
Size: 250 800 4933
Fragments arranged by size:
4933 800 250
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SexAI A'CCwGG_T
Cuts at: 3204 3204
Size: 5983
SfcI C'TryA_G
Cuts at: 826 1045 2899 2996 3331 4466 5147 5338 826
Size: 219 1854 97 335 1135 681 191 1471
Fragments arranged by size:
1854 1471 1135 681 335 219 191 97
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4087 4988 5250 820
Size: 197 926 2144 901 262 1553
Fragments arranged by size:
2144 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 2180 2507 1517
Size: 663 327 4993
Fragments arranged by size:
4993 663 327
StyI C'CwwG_G
Cuts at: 513 1065 2120 3422 513
Size: 552 1055 1302 3074
Fragments arranged by size:
3074 1302 1055 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4010 4027 4180 4365 4810 325
Size: 1255 2430 17 153 185 445 1498
Fragments arranged by size:
2430 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4229 5941 96
Size: 274 80 33 51 1443 2252 1712 138
Fragments arranged by size:
2252 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3142 3174 5943 5975 3142
Size: 32 2769 32 3150
Fragments arranged by size:
3150 2769 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3206 1316
Size: 1890 4093
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2082 2082
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2813 3063 4112 2813
Size: 250 1049 4684
Fragments arranged by size:
4684 1049 250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspXI PsrI SacI SacII SanDI SfiI SfoI
SrfI SwaI