Restriction map for
pFN21AB7710
(Circular) (Six-base) MAPSORT of: pFN21AB7710.seq Check: 514 from: 1 to: 6100
pFN21AB7710 6100 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2724 3268 2724
Size: 544 5556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2401 3443 1937
Size: 464 1042 4594
Fragments arranged by size:
4594 1042 464
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 4163 5547 5815 1632
Size: 413 2118 1384 268 1917
Fragments arranged by size:
2118 1917 1384 413 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 4931 4931
Size: 6100
AhdI GACnn_n'nnGTC
Cuts at: 2944 4829 2944
Size: 1885 4215
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2183 3236 1184
Size: 999 1053 4048
Fragments arranged by size:
4048 1053 999
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3361 3393 3361
Size: 32 6068
AlwNI CAG_nnn'CTG
Cuts at: 2542 3843 5415 2542
Size: 1301 1572 3227
Fragments arranged by size:
3227 1572 1301
ApaI G_GGCC'C
Cuts at: 2149 2234 2149
Size: 85 6015
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3409 3635 3787 5896 1493
Size: 1916 226 152 2109 1697
Fragments arranged by size:
2109 1916 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2155 3028 3208 3425 1870
Size: 73 212 873 180 217 4545
Fragments arranged by size:
4545 873 217 212 180 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3045 3212 3421 4876 618
Size: 325 217 627 1258 167 209 1455 1842
Fragments arranged by size:
1842 1455 1258 627 325 217 209 167
BanII G_rGCy'C
Cuts at: 1391 2149 2234 2659 3312 3419 1391
Size: 758 85 425 653 107 4072
Fragments arranged by size:
4072 758 653 425 107 85
BbeI G_GCGC'C
Cuts at: 3049 3049
Size: 6100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2463 2753 2943 3340 928
Size: 971 564 290 190 397 3688
Fragments arranged by size:
3688 971 564 397 290 190
BbvCI CC'TCA_GC
Cuts at: 2576 2576
Size: 6100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2276 2310 4291 4325 1903
Size: 34 50 34 255 34 1981 34 3678
Fragments arranged by size:
3678 1981 255 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 3171 1548
Size: 1623 4477
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4711 136
Size: 107 122 71 631 605 3039 1525
Fragments arranged by size:
3039 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2271 6095 2271
Size: 3824 2276
BlpI GC'TnA_GC
Cuts at: 3227 3517 3227
Size: 290 5810
Bme1580I G_kGCm'C
Cuts at: 1790 2149 2226 2234 3215 4161 5317 1790
Size: 359 77 8 981 946 1156 2573
Fragments arranged by size:
2573 1156 981 946 359 77 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6100
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3831 4789 442
Size: 416 53 83 399 24 2414 958 1753
Fragments arranged by size:
2414 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2564 2606 2693 4760 1239
Size: 854 471 42 87 2067 2579
Fragments arranged by size:
2579 2067 854 471 87 42
Bpu10I CC'TnA_GC
Cuts at: 2576 2576
Size: 6100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4225 5090 5388 4225
Size: 865 298 4937
Fragments arranged by size:
4937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2635 4763 882
Size: 617 1136 2128 2219
Fragments arranged by size:
2219 2128 1136 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3046 3159 3907 4289
Size: 53 83 186 936 1513 113 748 382
Cuts at: 4289 4924 275
Size: 635 1451
Fragments arranged by size:
1513 1451 936 748 635 382 186 113
83 53
BsaWI w'CCGG_w
Cuts at: 4533 4931 5205 5352 5763 4533
Size: 398 274 147 411 4870
Fragments arranged by size:
4870 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2646 2666 2676 2696 2808
Size: 30 165 30 1243 20 10 20 112
Cuts at: 2808 2838 1178
Size: 30 4440
Fragments arranged by size:
4440 1243 165 112 30 30 30 20
20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2207 2270 2690 3028 3136 1186
Size: 1018 3 63 420 338 108 4150
Fragments arranged by size:
4150 1018 420 338 108 63 3
BseYI C'CCAG_C
Cuts at: 2236 3073 3192 5303 5799 2236
Size: 837 119 2111 496 2537
Fragments arranged by size:
2537 2111 837 496 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3076 1163
Size: 573 1340 4187
Fragments arranged by size:
4187 1340 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2420 4161 4246 5317 2262
Size: 158 1741 85 1071 3045
Fragments arranged by size:
3045 1741 1071 158 85
BsmI GAATG_Cn'
Cuts at: 3611 3704 3611
Size: 93 6007
BsmBI CGTCTCn'nnnn_
Cuts at: 2396 2396
Size: 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2149 2226 2234 2262 2420 2659 3215
Size: 399 359 77 8 28 158 239 556
Cuts at: 3215 3312 3419 4161 4246 5317 1391
Size: 97 107 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 399 359 239 158
107 97 85 77 28 8
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2724 3049 3075 3228 3268 3455 844
Size: 1880 325 26 153 40 187 3489
Fragments arranged by size:
3489 1880 325 187 153 40 26
BsrBI CCG'CTC
Cuts at: 1394 1823 2179 3987 1394
Size: 429 356 1808 3507
Fragments arranged by size:
3507 1808 429 356
BsrDI GCAATG_nn'
Cuts at: 65 1248 4595 4769 65
Size: 1183 3347 174 1396
Fragments arranged by size:
3347 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2112 4744 4931 1700
Size: 110 302 2632 187 2869
Fragments arranged by size:
2869 2632 302 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6100
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 2083 3280 1184
Size: 899 1197 4004
Fragments arranged by size:
4004 1197 899
BstYI r'GATC_y
Cuts at: 1972 2163 2271 2793 3234 3430 3797 4182 4199
Size: 191 108 522 441 196 367 385 17
Cuts at: 4199 5651 5737 5749 6095 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 522 441 385 367 346 196
191 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2763 2763
Size: 6100
BtgI C'CryG_G
Cuts at: 513 1065 2211 2349 513
Size: 552 1146 138 4264
Fragments arranged by size:
4264 1146 552 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2569 487
Size: 683 56 1343 4018
Fragments arranged by size:
4018 1343 683 56
BtsI GCAGTG_nn'
Cuts at: 3194 3324 3616 4410 4430 3194
Size: 130 292 794 20 4864
Fragments arranged by size:
4864 794 292 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DraIII CAC_nnn'GTG
Cuts at: 2350 2350
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2568 3105 3547
Size: 54 1425 50 275 18 738 537 442
Cuts at: 3547 4436 6065 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 738 537 442 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2966 4030 1353
Size: 1613 1064 3423
Fragments arranged by size:
3423 1613 1064
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2093 2564 2606 2693 4163 4760
Size: 393 413 48 471 42 87 1470 597
Cuts at: 4760 5547 5815 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1470 787 597 471 413 393 268
87 48 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2363 2363
Size: 6100
EcoO109I rG'GnC_Cy
Cuts at: 2145 2327 2145
Size: 182 5918
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 6100
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 3049 3150 5247 1678
Size: 1371 101 2097 2531
Fragments arranged by size:
2531 2097 1371 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2646 2678 2930 2962 4741
Size: 32 135 32 1074 32 252 32 1779
Cuts at: 4741 4773 4815 4847 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1779 1074 252 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2402 2725 3064 3444 3690 669
Size: 988 281 464 323 339 380 246 3079
Fragments arranged by size:
3079 988 464 380 339 323 281 246
HindIII A'AGCT_T
Cuts at: 748 2465 748
Size: 1717 4383
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KasI G'GCGC_C
Cuts at: 3045 3045
Size: 6100
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5214 5398 1305
Size: 3909 184 2007
Fragments arranged by size:
3909 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 10
Size: 54 1425 343 738 3540
Fragments arranged by size:
3540 1425 738 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4058 4417 4576 518
Size: 648 2892 359 159 2042
Fragments arranged by size:
2892 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2236 3054 3073 3226 3516 4193 5341
Size: 465 165 818 19 153 290 677 1148
Cuts at: 5341 5586 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 818 677 465 290 245 165
153 19
NaeI GCC'GGC
Cuts at: 2114 2114
Size: 6100
NarI GG'CG_CC
Cuts at: 3046 3046
Size: 6100
NcoI C'CATG_G
Cuts at: 513 1065 2211 513
Size: 552 1146 4402
Fragments arranged by size:
4402 1146 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NgoMIV G'CCGG_C
Cuts at: 2112 2112
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 3458 5003 1132
Size: 97 2229 1545 2229
Fragments arranged by size:
2229 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2227 1348
Size: 879 5221
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6100
PfoI T'CCnGG_A
Cuts at: 2358 2358
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2327 2327
Size: 6100
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspOMI G'GGCC_C
Cuts at: 2145 2230 2145
Size: 85 6015
PstI C_TGCA'G
Cuts at: 830 2321 2535 3072 3452 830
Size: 1491 214 537 380 3478
Fragments arranged by size:
3478 1491 537 380 214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 3073 3073
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 2400 3442 1936
Size: 464 1042 4594
Fragments arranged by size:
4594 1042 464
SanDI GG'GwC_CC
Cuts at: 2327 2327
Size: 6100
SapI GCTCTTCn'nnn_
Cuts at: 2966 2966
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 3452 3452
Size: 6100
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2317 2531 3068 3448 4583 5264 5455
Size: 219 1272 214 537 380 1135 681 191
Cuts at: 5455 826
Size: 1471
Fragments arranged by size:
1471 1272 1135 681 537 380 219 214
191
SfoI GGC'GCC
Cuts at: 3047 3047
Size: 6100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3030 3210 3427 1872
Size: 1158 180 217 4545
Fragments arranged by size:
4545 1158 217 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4204 5105 5367 820
Size: 197 926 2261 901 262 1553
Fragments arranged by size:
2261 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 2211 3539 513
Size: 552 1146 1328 3074
Fragments arranged by size:
3074 1328 1146 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4127 4144 4297 4482 4927 325
Size: 1255 2547 17 153 185 445 1498
Fragments arranged by size:
2547 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4346 6058 96
Size: 274 80 33 51 1443 2369 1712 138
Fragments arranged by size:
2369 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2432 2464 2666 2698 6060 6092 2432
Size: 32 202 32 3362 32 2440
Fragments arranged by size:
3362 2440 202 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2783 3158 1316
Size: 1467 375 4258
Fragments arranged by size:
4258 1467 375
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2920 2920
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 3028 3208 3425 1870
Size: 1158 180 217 4545
Fragments arranged by size:
4545 1158 217 180
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI
SrfI SwaI