Restriction map for
pFN21AB7709
(Circular) (Six-base) MAPSORT of: pFN21AB7709.seq Check: 3862 from: 1 to: 5965
pFN21AB7709 5965 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3105 3308 1937
Size: 1168 203 4594
Fragments arranged by size:
4594 1168 203
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2078 4028 5412 5680 1632
Size: 446 1950 1384 268 1917
Fragments arranged by size:
1950 1917 1384 446 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2929 1184
Size: 1745 4220
AlwNI CAG_nnn'CTG
Cuts at: 2777 3708 5280 2777
Size: 931 1572 3462
Fragments arranged by size:
3462 1572 931
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 2130 3274 3500 3652 5761 1493
Size: 637 1144 226 152 2109 1697
Fragments arranged by size:
2109 1697 1144 637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2498 4519 160
Size: 872 1466 2021 1606
Fragments arranged by size:
2021 1606 1466 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 3290 1870
Size: 73 1347 4545
Fragments arranged by size:
4545 1347 73
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3286 4741 618
Size: 325 217 627 1499 1455 1842
Fragments arranged by size:
1842 1499 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3284 1391
Size: 1893 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2696 928
Size: 971 797 4197
Fragments arranged by size:
4197 971 797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4156 4190 1903
Size: 34 50 34 2135 34 3678
Fragments arranged by size:
3678 2135 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 2164 1548
Size: 616 5349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4576 136
Size: 107 122 71 631 605 2904 1525
Fragments arranged by size:
2904 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2086 2953 5960 2086
Size: 867 3007 2091
Fragments arranged by size:
3007 2091 867
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 4026 5182 1790
Size: 2236 1156 2573
Fragments arranged by size:
2573 2236 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2765 3696 4654
Size: 416 53 83 399 24 1348 931 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1348 958 931 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2845 3068 4625 1239
Size: 1606 223 1557 2579
Fragments arranged by size:
2579 1606 1557 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2705 4090 4955 5253 2705
Size: 1385 865 298 3417
Fragments arranged by size:
3417 1385 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2713 4628 882
Size: 617 1214 1915 2219
Fragments arranged by size:
2219 1915 1214 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3772 4154 4789 275
Size: 53 83 186 936 2239 382 635 1451
Fragments arranged by size:
2239 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4398 4796 5070 5217 5628 4398
Size: 398 274 147 411 4735
Fragments arranged by size:
4735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5740
Fragments arranged by size:
5740 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2518 3034 1186
Size: 1332 516 4117
Fragments arranged by size:
4117 1332 516
BseYI C'CCAG_C
Cuts at: 2242 5168 5664 2242
Size: 2926 496 2543
Fragments arranged by size:
2926 2543 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3089 1163
Size: 573 1353 4039
Fragments arranged by size:
4039 1353 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3415 4176 5204 1540
Size: 275 186 1414 761 1028 2301
Fragments arranged by size:
2301 1414 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4026 4111 5182 4026
Size: 85 1071 4809
Fragments arranged by size:
4809 1071 85
BsmI GAATG_Cn'
Cuts at: 2284 3476 3569 2284
Size: 1192 93 4680
Fragments arranged by size:
4680 1192 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3284 4026 4111 5182 1391
Size: 399 1494 742 85 1071 2174
Fragments arranged by size:
2174 1494 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 844
Size: 2476 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 1394
Size: 429 2029 3507
Fragments arranged by size:
3507 2029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2923 4460 4634 65
Size: 1183 1675 1537 174 1396
Fragments arranged by size:
1675 1537 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4609 4796 1700
Size: 110 2799 187 2869
Fragments arranged by size:
2869 2799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5965
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2576 794
Size: 1782 4183
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 2086 2607 2953 3295 3662 4047 4064 5516
Size: 114 521 346 342 367 385 17 1452
Cuts at: 5516 5602 5614 5960 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 521 385 367 346 346 342
114 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 2521 3481 4275 4295 2521
Size: 960 794 20 4191
Fragments arranged by size:
4191 960 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2811 4972 809
Size: 507 1495 2161 1802
Fragments arranged by size:
2161 1802 1495 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3412 4301 5930
Size: 54 1425 50 275 18 1582 889 1629
Cuts at: 5930 8
Size: 43
Fragments arranged by size:
1629 1582 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 3895 1353
Size: 1104 1438 3423
Fragments arranged by size:
3423 1438 1104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4552 4926 5072 387
Size: 1064 138 2963 374 146 1280
Fragments arranged by size:
2963 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2845 3068 4028 4625 5412 5680
Size: 393 446 767 223 960 597 787 268
Cuts at: 5680 1239
Size: 1524
Fragments arranged by size:
1524 960 787 767 597 446 393 268
223
EcoO109I rG'GnC_Cy
Cuts at: 2467 2743 3250 2467
Size: 276 507 5182
Fragments arranged by size:
5182 507 276
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2561 2561
Size: 5965
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 2706 5112 1678
Size: 1028 2406 2531
Fragments arranged by size:
2531 2406 1028
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 3152 3184 4606
Size: 32 135 32 791 32 757 32 1422
Cuts at: 4606 4638 4680 4712 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1422 791 757 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 3196 3309 3555 669
Size: 988 281 1168 90 113 246 3079
Fragments arranged by size:
3079 1168 988 281 246 113 90
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5965
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2821 5079 5263 1305
Size: 1516 2258 184 2007
Fragments arranged by size:
2258 2007 1516 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4143
Fragments arranged by size:
4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3923 4282 4441 518
Size: 648 2757 359 159 2042
Fragments arranged by size:
2757 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3381 4058 5206 5451 1606
Size: 1775 677 1148 245 2120
Fragments arranged by size:
2120 1775 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5413
NdeI CA'TA_TG
Cuts at: 387 2218 387
Size: 1831 4134
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2143 1108
Size: 1035 4930
PfoI T'CCnGG_A
Cuts at: 3045 3076 3045
Size: 31 5934
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2743 2467
Size: 276 5689
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PstI C_TGCA'G
Cuts at: 830 3317 830
Size: 2487 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3104 3307 1936
Size: 1168 203 4594
Fragments arranged by size:
4594 1168 203
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 2230 4213 5925 2230
Size: 1983 1712 2270
Fragments arranged by size:
2270 1983 1712
SfcI C'TryA_G
Cuts at: 826 1045 2256 2262 3313 4448 5129 5320 826
Size: 219 1211 6 1051 1135 681 191 1471
Fragments arranged by size:
1471 1211 1135 1051 681 219 191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 4069 4970 5232 820
Size: 197 926 741 1385 901 262 1553
Fragments arranged by size:
1553 1385 926 901 741 262 197
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 3031 3889 5
Size: 47 2979 858 2081
Fragments arranged by size:
2979 2081 858 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2142 3404 513
Size: 552 1077 1262 3074
Fragments arranged by size:
3074 1262 1077 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3992 4009 4162 4347 4792 325
Size: 1255 2412 17 153 185 445 1498
Fragments arranged by size:
2412 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2228 4211 5923
Size: 274 80 33 51 1443 251 1983 1712
Cuts at: 5923 96
Size: 138
Fragments arranged by size:
1983 1712 1443 274 251 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 3301 3301
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI