Restriction map for
pFN21AB7704
(Circular) (Six-base) MAPSORT of: pFN21AB7704.seq Check: 5277 from: 1 to: 6091
pFN21AB7704 6091 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2109 3434 1937
Size: 172 1325 4594
Fragments arranged by size:
4594 1325 172
Acc65I G'GTAC_C
Cuts at: 3161 3412 3161
Size: 251 5840
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2022 2844 2877 3114 3404 4154 5538 5806
Size: 390 822 33 237 290 750 1384 268
Cuts at: 5806 1632
Size: 1917
Fragments arranged by size:
1917 1384 822 750 390 290 268 237
33
AflII C'TTAA_G
Cuts at: 820 1017 2681 820
Size: 197 1664 4230
Fragments arranged by size:
4230 1664 197
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 2116 4820 2116
Size: 2704 3387
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 2551 4148 5304 2551
Size: 1597 1156 3338
Fragments arranged by size:
3338 1597 1156
ApoI r'AATT_y
Cuts at: 1493 2100 3183 3400 3626 3778 5887 1493
Size: 607 1083 217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1083 607 226 217 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2914 3416 1870
Size: 73 72 899 502 4545
Fragments arranged by size:
4545 899 502 73 72
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 2415 3161 3412 4867
Size: 325 217 627 378 250 746 251 1455
Cuts at: 4867 618
Size: 1842
Fragments arranged by size:
1842 1455 746 627 378 325 251 250
217
BanII G_rGCy'C
Cuts at: 1391 3410 1391
Size: 2019 4072
BbeI G_GCGC'C
Cuts at: 2419 2419
Size: 6091
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3069 928
Size: 971 1170 3950
Fragments arranged by size:
3950 1170 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2413 4702 136
Size: 107 122 71 631 605 741 2289 1525
Fragments arranged by size:
2289 1525 741 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2457 3508 2457
Size: 1051 5040
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2555 4152 5308 1790
Size: 225 540 1597 1156 2573
Fragments arranged by size:
2573 1597 1156 540 225
BmgBI CAC'GTC
Cuts at: 1318 2785 1318
Size: 1467 4624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2565 2763 3329 4751 1239
Size: 1326 198 566 1422 2579
Fragments arranged by size:
2579 1422 1326 566 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3269 3338 4216 5081 5379 3269
Size: 69 878 865 298 3981
Fragments arranged by size:
3981 878 865 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2416 3898 4280 4915
Size: 53 83 186 936 883 1482 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1482 1451 936 883 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2706 4524 4922 5196 5343 5754 2706
Size: 1818 398 274 147 411 3043
Fragments arranged by size:
3043 1818 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3089 3092 1186
Size: 1903 3 4185
Fragments arranged by size:
4185 1903 3
BseYI C'CCAG_C
Cuts at: 5294 5790 5294
Size: 496 5595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2113 3541 4302 5330 1540
Size: 275 186 112 1428 761 1028 2301
Fragments arranged by size:
2301 1428 1028 761 275 186 112
BsiHKAI G_wGCw'C
Cuts at: 2555 4152 4237 5308 2555
Size: 1597 85 1071 3338
Fragments arranged by size:
3338 1597 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2555 3410 4152 4237 5308 1391
Size: 399 225 540 855 742 85 1071 2174
Fragments arranged by size:
2174 1071 855 742 540 399 225 85
BspEI T'CCGG_A
Cuts at: 2706 5754 2706
Size: 3048 3043
BspHI T'CATG_A
Cuts at: 3261 3980 5710 3261
Size: 719 1730 3642
Fragments arranged by size:
3642 1730 719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2148 2850 4586 4760 65
Size: 1183 900 702 1736 174 1396
Fragments arranged by size:
1736 1396 1183 900 702 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 3253 3421 3788 4173 4190 5642 5728 5740
Size: 1281 168 367 385 17 1452 86 12
Cuts at: 5740 6086 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1281 385 367 346 168 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2113 513
Size: 552 939 109 4491
Fragments arranged by size:
4491 939 552 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 2553 3607 4401 4421 2553
Size: 1054 794 20 4223
Fragments arranged by size:
4223 1054 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3139 3538 4427
Size: 54 1425 50 275 18 1309 399 889
Cuts at: 4427 6056 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1309 889 399 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3066 3090 4021 1353
Size: 1713 24 931 3423
Fragments arranged by size:
3423 1713 931 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2565 2763 2844 2877 3114 3329
Size: 393 390 543 198 81 33 237 215
Cuts at: 3329 3404 4154 4751 5538 5806 1239
Size: 75 750 597 787 268 1524
Fragments arranged by size:
1524 787 750 597 543 393 390 268
237 215 198 81 75 33
EcoO109I rG'GnC_Cy
Cuts at: 2233 2725 2233
Size: 492 5599
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
EcoRV GAT'ATC
Cuts at: 2569 2910 2569
Size: 341 5750
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 2145 2217 2419 5238 1678
Size: 467 72 202 2819 2531
Fragments arranged by size:
2819 2531 467 202 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 4732 4764 4806
Size: 32 135 32 530 32 2598 32 42
Cuts at: 4806 4838 1373
Size: 32 2626
Fragments arranged by size:
2626 2598 530 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2110 2467 3435 3681 669
Size: 988 281 172 357 968 246 3079
Fragments arranged by size:
3079 988 968 357 281 246 172
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 2415 2415
Size: 6091
KpnI G_GTAC'C
Cuts at: 3165 3416 3165
Size: 251 5840
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3141 10
Size: 54 1425 343 1309 2960
Fragments arranged by size:
2960 1425 1309 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3266 4049 4408 4567 518
Size: 648 2100 783 359 159 2042
Fragments arranged by size:
2100 2042 783 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2594 3115 3507 4184 5332 5577 1606
Size: 988 521 392 677 1148 245 2120
Fragments arranged by size:
2120 1148 988 677 521 392 245
NarI GG'CG_CC
Cuts at: 2416 2416
Size: 6091
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2336 3449 4994 1132
Size: 97 1107 1113 1545 2229
Fragments arranged by size:
2229 1545 1113 1107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PfoI T'CCnGG_A
Cuts at: 2247 2247
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2725
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 2520 3661 2520
Size: 1141 4950
PstI C_TGCA'G
Cuts at: 830 3120 3443 830
Size: 2290 323 3478
Fragments arranged by size:
3478 2290 323
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2594 3115 2594
Size: 521 5570
SalI G'TCGA_C
Cuts at: 1936 2108 3433 1936
Size: 172 1325 4594
Fragments arranged by size:
4594 1325 172
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 3116 3359 3439 4574 5255 5446 826
Size: 219 2071 243 80 1135 681 191 1471
Fragments arranged by size:
2071 1471 1135 681 243 219 191 80
SfoI GGC'GCC
Cuts at: 2417 2417
Size: 6091
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2681 3284 3317 4195 5096 5358
Size: 197 926 738 603 33 878 901 262
Cuts at: 5358 820
Size: 1553
Fragments arranged by size:
1553 926 901 878 738 603 262 197
33
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2868 3530 513
Size: 552 939 864 662 3074
Fragments arranged by size:
3074 939 864 662 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 4118 4135 4288 4473 4918 325
Size: 1255 603 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 603 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2339 2766 4337
Size: 274 80 33 51 1443 362 427 1571
Cuts at: 4337 6049 96
Size: 1712 138
Fragments arranged by size:
1712 1571 1443 427 362 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2158 2190 6051 6083 2158
Size: 32 3861 32 2166
Fragments arranged by size:
3861 2166 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 2987 3427 2987
Size: 440 5651
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2702 4220 2702
Size: 1518 4573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI